stf62247 (MedChemExpress)
MedChemExpress is a verified supplier 
MedChemExpress manufactures this product 
94
Structured Review
MedChemExpress
stf62247
Stf62247, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stf62247/STF-62247/pm37789342-46-26-34
Average 94 stars, based on 2 article reviews
Stf62247, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stf62247/STF-62247/pm37789342-46-26-34
Average 94 stars, based on 2 article reviews
stf62247 - by Bioz Stars,
2026-10
94/100 stars
Images
1) Product Images from "Autophagy-amplifying nanoparticles evoke immunogenic cell death combined with anti-PD-1/PD-L1 for residual tumors immunotherapy after RFA."
Article Title: Autophagy-amplifying nanoparticles evoke immunogenic cell death combined with anti-PD-1/PD-L1 for residual tumors immunotherapy after RFA.
Journal: Journal of nanobiotechnology
doi: 10.1186/s12951-023-02067-y
Figure Legend Snippet: Fig. 2 Preparation and Characterization of STF-BMS@ZIF-8/PEG-FA. A, B TEM images of ZIF-8 and STF-BMS@ZIF-8/PEG-FA. Scale: 20 nm. C, D TEM test the particle size distribution of ZIF-8 and STF-BMS@ZIF-8/PEG-FA. E Ultraviolet–visible spectroscopy of ZIF-8, STF, STF-BMS@ZIF-8, STF-BMS@ ZIF-8/PEG-FA. F Zeta potentials of ZIF-8, STF-BMS@ZIF-8, and STF-BMS@ZIF-8/PEG-FA (n = 3). G, H pH-responsive release percentage of STF and BMS from STF-BMS@ZIF8/PEG-FA in different buffers at pH 5.5 and 7.4. I XRD of ZIF-8, STF-BMS@ZIF-8, and STF-BMS@ZIF-8/PEG-FA. J FITR of ZIF-8, STF-BMS@ZIF-8, and STF-BMS@ZIF-8/PEG-FA. K Thermogravimetric analysis of ZIF-8 and STF-BMS@ZIF-8/PEG-FA. L XPS spectra of ZIF-8, STF-BMS@ ZIF-8, and STF-BMS@ZIF-8/PEG-FA (C1s, N1s, O1s, S2p). ZIF-8, zeolitic imidazolate framework-8; STF, STF62247; BMS202, BMS; SBZ, STF- BMS@ZIF-8; SBZP, STF- BMS @ZIF-8@PEG-FA; TEM, Transmission emission micrography; FTIR, Fourier transform IR; XRD, X-ray diffraction; XPS, X-ray photoelectron spectroscopy; FITC, Fluorescein Isothiocyanate
Techniques Used: Spectroscopy, Transmission Assay
Figure Legend Snippet: Fig. 4 SZP enhances autophagy in sublethally heated HCC cells. A Diagram illustrating how autophagy was induced by SZP. B The ultrastructure of tumor cells after SZP NPs treatment observed via TEM, with autophagosomes and autolysosomes pointed out by arrows. Scale: 1 µm. C Immunoblotting of LC3B in SMMC7721 and Huh7 cells pre-treated with 3MA under sublethal heat stress caused by SZP. D Immunoblotting of LC3B and P62 in SMMC7721 and Huh7 cells pre-treated with CQ under sublethal heat stress induced by SZP. E–G Representative CLSM images and quantitative analysis of HCC cells transfected with mRFP-eGFP-LC3 plasmid under the influence of different groups (arrow pointing towards green spots, red spots, and yellow spots). Scale: 20 µm. Results were expressed as mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001. SZP, STF62247@ZIF-8/PEG-FA; TEM, Transmission Electron Microscope; 3MA, 3-Methyladenine; CQ, Chloroquine
Techniques Used: Western Blot, Transfection, Plasmid Preparation, Transmission Assay, Microscopy
Figure Legend Snippet: Fig. 5 SZP mediates ACD. A The dose–response curve for CCK8 assay of IC50 of STF62247 in different concentrations treating cells at 37 °C and 47 °C for 24 h (n = 3). B The dose–response curve for CCK8 assay of IC50 of STF62247 and SZP NPs in different concentrations treating HCC cells at 47 °C for 24 h (n = 3). C, F Live/dead cells double staining and quantitative analysis of sublethally heated SMMC7721 and Huh7 cells exposed to different groups (n = 4). Scale: 1000 µm. D Analysis of the proliferation of sublethally heated SMMC7721 and Huh7 cells under influence of different groups by CCK8 (n = 3). E, G Representative flow cytometry plots and quantitative analysis of apoptosis in sublethally heated SMMC7721 and Huh7 cells under influence of different conditions (n = 3). H Immunoblotting of p-mTOR, t-mTOR, P62, and LC3B in sublethally heated SMMC7721 and Huh7 cells under influence of different groups. The results were expressed as mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001. ACD, Autophagy-dependent cell death; IC50, Half maximal inhibitory concentration
Techniques Used: CCK-8 Assay, Double Staining, Flow Cytometry, Western Blot, Concentration Assay
Related Articles
Spectroscopy:Article Title: Autophagy-amplifying nanoparticles evoke immunogenic cell death combined with anti-PD-1/PD-L1 for residual tumors immunotherapy after RFA. Article Snippet: Zn (NO3)2•6H2O and 2-MeIM were purchased from Aladdin Holdings Group Co., Ltd (Shanghai); PEGFA 2000, ICG, and FITC from Rixi Biotechnology Co., Ltd. (Xi’an); BMS202 (HY-19745), Article Title: Autophagy-amplifying nanoparticles evoke immunogenic cell death combined with anti-PD-1/PD-L1 for residual tumors immunotherapy after RFA Article Snippet: Zn (NO3)2•6H2O and 2-MeIM were purchased from Aladdin Holdings Group Co., Ltd (Shanghai); PEG-FA 2000, ICG, and FITC from Rixi Biotechnology Co., Ltd. (Xi’an); BMS202 (HY-19745), Transmission Assay:Article Title: Autophagy-amplifying nanoparticles evoke immunogenic cell death combined with anti-PD-1/PD-L1 for residual tumors immunotherapy after RFA. Article Snippet: Zn (NO3)2•6H2O and 2-MeIM were purchased from Aladdin Holdings Group Co., Ltd (Shanghai); PEGFA 2000, ICG, and FITC from Rixi Biotechnology Co., Ltd. (Xi’an); BMS202 (HY-19745), Article Title: Autophagy-amplifying nanoparticles evoke immunogenic cell death combined with anti-PD-1/PD-L1 for residual tumors immunotherapy after RFA Article Snippet: Zn (NO3)2•6H2O and 2-MeIM were purchased from Aladdin Holdings Group Co., Ltd (Shanghai); PEG-FA 2000, ICG, and FITC from Rixi Biotechnology Co., Ltd. (Xi’an); BMS202 (HY-19745), Western Blot:Article Title: Autophagy-amplifying nanoparticles evoke immunogenic cell death combined with anti-PD-1/PD-L1 for residual tumors immunotherapy after RFA. Article Snippet: Zn (NO3)2•6H2O and 2-MeIM were purchased from Aladdin Holdings Group Co., Ltd (Shanghai); PEGFA 2000, ICG, and FITC from Rixi Biotechnology Co., Ltd. (Xi’an); BMS202 (HY-19745), Article Title: Autophagy-amplifying nanoparticles evoke immunogenic cell death combined with anti-PD-1/PD-L1 for residual tumors immunotherapy after RFA Article Snippet: Zn (NO3)2•6H2O and 2-MeIM were purchased from Aladdin Holdings Group Co., Ltd (Shanghai); PEG-FA 2000, ICG, and FITC from Rixi Biotechnology Co., Ltd. (Xi’an); BMS202 (HY-19745), Transfection:Article Title: Autophagy-amplifying nanoparticles evoke immunogenic cell death combined with anti-PD-1/PD-L1 for residual tumors immunotherapy after RFA. Article Snippet: Zn (NO3)2•6H2O and 2-MeIM were purchased from Aladdin Holdings Group Co., Ltd (Shanghai); PEGFA 2000, ICG, and FITC from Rixi Biotechnology Co., Ltd. (Xi’an); BMS202 (HY-19745), Article Title: Autophagy-amplifying nanoparticles evoke immunogenic cell death combined with anti-PD-1/PD-L1 for residual tumors immunotherapy after RFA Article Snippet: Zn (NO3)2•6H2O and 2-MeIM were purchased from Aladdin Holdings Group Co., Ltd (Shanghai); PEG-FA 2000, ICG, and FITC from Rixi Biotechnology Co., Ltd. (Xi’an); BMS202 (HY-19745), Plasmid Preparation:Article Title: Autophagy-amplifying nanoparticles evoke immunogenic cell death combined with anti-PD-1/PD-L1 for residual tumors immunotherapy after RFA. Article Snippet: Zn (NO3)2•6H2O and 2-MeIM were purchased from Aladdin Holdings Group Co., Ltd (Shanghai); PEGFA 2000, ICG, and FITC from Rixi Biotechnology Co., Ltd. (Xi’an); BMS202 (HY-19745), Article Title: Autophagy-amplifying nanoparticles evoke immunogenic cell death combined with anti-PD-1/PD-L1 for residual tumors immunotherapy after RFA Article Snippet: Zn (NO3)2•6H2O and 2-MeIM were purchased from Aladdin Holdings Group Co., Ltd (Shanghai); PEG-FA 2000, ICG, and FITC from Rixi Biotechnology Co., Ltd. (Xi’an); BMS202 (HY-19745), Microscopy:Article Title: Autophagy-amplifying nanoparticles evoke immunogenic cell death combined with anti-PD-1/PD-L1 for residual tumors immunotherapy after RFA. Article Snippet: Zn (NO3)2•6H2O and 2-MeIM were purchased from Aladdin Holdings Group Co., Ltd (Shanghai); PEGFA 2000, ICG, and FITC from Rixi Biotechnology Co., Ltd. (Xi’an); BMS202 (HY-19745), Article Title: Autophagy-amplifying nanoparticles evoke immunogenic cell death combined with anti-PD-1/PD-L1 for residual tumors immunotherapy after RFA Article Snippet: Zn (NO3)2•6H2O and 2-MeIM were purchased from Aladdin Holdings Group Co., Ltd (Shanghai); PEG-FA 2000, ICG, and FITC from Rixi Biotechnology Co., Ltd. (Xi’an); BMS202 (HY-19745), CCK-8 Assay:Article Title: Autophagy-amplifying nanoparticles evoke immunogenic cell death combined with anti-PD-1/PD-L1 for residual tumors immunotherapy after RFA. Article Snippet: Zn (NO3)2•6H2O and 2-MeIM were purchased from Aladdin Holdings Group Co., Ltd (Shanghai); PEGFA 2000, ICG, and FITC from Rixi Biotechnology Co., Ltd. (Xi’an); BMS202 (HY-19745), Article Title: Autophagy-amplifying nanoparticles evoke immunogenic cell death combined with anti-PD-1/PD-L1 for residual tumors immunotherapy after RFA Article Snippet: Zn (NO3)2•6H2O and 2-MeIM were purchased from Aladdin Holdings Group Co., Ltd (Shanghai); PEG-FA 2000, ICG, and FITC from Rixi Biotechnology Co., Ltd. (Xi’an); BMS202 (HY-19745), Double Staining:Article Title: Autophagy-amplifying nanoparticles evoke immunogenic cell death combined with anti-PD-1/PD-L1 for residual tumors immunotherapy after RFA. Article Snippet: Zn (NO3)2•6H2O and 2-MeIM were purchased from Aladdin Holdings Group Co., Ltd (Shanghai); PEGFA 2000, ICG, and FITC from Rixi Biotechnology Co., Ltd. (Xi’an); BMS202 (HY-19745), Article Title: Autophagy-amplifying nanoparticles evoke immunogenic cell death combined with anti-PD-1/PD-L1 for residual tumors immunotherapy after RFA Article Snippet: Zn (NO3)2•6H2O and 2-MeIM were purchased from Aladdin Holdings Group Co., Ltd (Shanghai); PEG-FA 2000, ICG, and FITC from Rixi Biotechnology Co., Ltd. (Xi’an); BMS202 (HY-19745), Flow Cytometry:Article Title: Autophagy-amplifying nanoparticles evoke immunogenic cell death combined with anti-PD-1/PD-L1 for residual tumors immunotherapy after RFA. Article Snippet: Zn (NO3)2•6H2O and 2-MeIM were purchased from Aladdin Holdings Group Co., Ltd (Shanghai); PEGFA 2000, ICG, and FITC from Rixi Biotechnology Co., Ltd. (Xi’an); BMS202 (HY-19745), Article Title: Autophagy-amplifying nanoparticles evoke immunogenic cell death combined with anti-PD-1/PD-L1 for residual tumors immunotherapy after RFA Article Snippet: Zn (NO3)2•6H2O and 2-MeIM were purchased from Aladdin Holdings Group Co., Ltd (Shanghai); PEG-FA 2000, ICG, and FITC from Rixi Biotechnology Co., Ltd. (Xi’an); BMS202 (HY-19745), Concentration Assay:Article Title: Autophagy-amplifying nanoparticles evoke immunogenic cell death combined with anti-PD-1/PD-L1 for residual tumors immunotherapy after RFA. Article Snippet: Zn (NO3)2•6H2O and 2-MeIM were purchased from Aladdin Holdings Group Co., Ltd (Shanghai); PEGFA 2000, ICG, and FITC from Rixi Biotechnology Co., Ltd. (Xi’an); BMS202 (HY-19745), Article Title: Autophagy-amplifying nanoparticles evoke immunogenic cell death combined with anti-PD-1/PD-L1 for residual tumors immunotherapy after RFA Article Snippet: Zn (NO3)2•6H2O and 2-MeIM were purchased from Aladdin Holdings Group Co., Ltd (Shanghai); PEG-FA 2000, ICG, and FITC from Rixi Biotechnology Co., Ltd. (Xi’an); BMS202 (HY-19745), |