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stem121  (TaKaRa)


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  • 96

    Structured Review

    TaKaRa stem121
    Stem121, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stem121/STEM123/pm40897897-566-5-7
    Average 96 stars, based on 46 article reviews
    stem121 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Immunohistochemical staining:

    Article Title: High‐Purity Functional Corneal Endothelial Cells From Human Induced Pluripotent Stem Cells via a Novel Wash‐Out Method
    Article Snippet: .. Immunohistochemical analysis was performed by incubation with primary antibodies against: N‐CADHERIN (1:200; Cell Signaling), ZO‐1 (1:150), STEM121 (1:500; Takara), CD4 (1:1000; Novus), CD11b (1:100; Abcam), and CD8 (1:1000; Abcam). .. The secondary antibodies included the corresponding Alexa‐488 or ‐Cy3‐labeled antibodies (1:500; Abcam).

    Article Title: High‐Purity Functional Corneal Endothelial Cells From Human Induced Pluripotent Stem Cells via a Novel Wash‐Out Method
    Article Snippet: .. Immunohistochemical analysis was performed by incubation with primary antibodies against: N-CADHERIN (1:200; Cell Signaling), ZO1 (1:150), STEM121 (1:500; Takara), CD4 (1:1000; Novus), CD11b (1:100; Abcam), and CD8 (1:1000; Abcam). .. The secondary antibodies included the corresponding Alexa-488 or -Cy3-labeled antibodies (1:500; Abcam).

    Article Title: Self-Organized Vascularized Cardiac Microtissues Derived from Human iPS Cells Promote Myocardial Repair through Functional Host–Graft Vascular Integration
    Article Snippet: Visualization was carried out using the conventional HRP/DAB method with Avidin-biotin-peroxidase complex (ABC-Elite, Vector Laboratories) (1:100). .. Immunohistochemical fluorescence staining for STEM121 and von Willebrand factor (vWF) were conducted, using a mouse monoclonal STEM121 antibody specific for human cytoplasmic marker (#Y40410; Takara Bio Inc., Kusatsu, Japan) (1:400) and a rabbit polyclonal anti-vWF antibody (#ab6994; Abcam) (1:1000) was used as the primary antibody, followed by an Alexa Fluor 546-conjugated anti-mouse secondary antibody (Thermo Fisher; 1:400) and an Alexa Fluor 488-conjugated anti-rabbit secondary antibody (Thermo Fisher; 1:400). ..

    Incubation:

    Article Title: High‐Purity Functional Corneal Endothelial Cells From Human Induced Pluripotent Stem Cells via a Novel Wash‐Out Method
    Article Snippet: .. Immunohistochemical analysis was performed by incubation with primary antibodies against: N‐CADHERIN (1:200; Cell Signaling), ZO‐1 (1:150), STEM121 (1:500; Takara), CD4 (1:1000; Novus), CD11b (1:100; Abcam), and CD8 (1:1000; Abcam). .. The secondary antibodies included the corresponding Alexa‐488 or ‐Cy3‐labeled antibodies (1:500; Abcam).

    Article Title: High‐Purity Functional Corneal Endothelial Cells From Human Induced Pluripotent Stem Cells via a Novel Wash‐Out Method
    Article Snippet: .. Immunohistochemical analysis was performed by incubation with primary antibodies against: N-CADHERIN (1:200; Cell Signaling), ZO1 (1:150), STEM121 (1:500; Takara), CD4 (1:1000; Novus), CD11b (1:100; Abcam), and CD8 (1:1000; Abcam). .. The secondary antibodies included the corresponding Alexa-488 or -Cy3-labeled antibodies (1:500; Abcam).

    Cell-Signaling:

    Article Title: Fluid shear stress activates a targetable mechano-metastatic cascade to promote medulloblastoma metastasis.
    Article Snippet: Biofluid flow generates fluid shear stress (FSS), a mechanical force widely present in the tissue microenvironment.. How brain tumour growth alters the conduit of biofluid and impacts FSS-regulated cancer progression is unknown.. Dissemination of medulloblastoma (MB) cells into the cerebrospinal fluid initiates metastasis within the central nervous system.

    Fluorescence:

    Article Title: Self-Organized Vascularized Cardiac Microtissues Derived from Human iPS Cells Promote Myocardial Repair through Functional Host–Graft Vascular Integration
    Article Snippet: Visualization was carried out using the conventional HRP/DAB method with Avidin-biotin-peroxidase complex (ABC-Elite, Vector Laboratories) (1:100). .. Immunohistochemical fluorescence staining for STEM121 and von Willebrand factor (vWF) were conducted, using a mouse monoclonal STEM121 antibody specific for human cytoplasmic marker (#Y40410; Takara Bio Inc., Kusatsu, Japan) (1:400) and a rabbit polyclonal anti-vWF antibody (#ab6994; Abcam) (1:1000) was used as the primary antibody, followed by an Alexa Fluor 546-conjugated anti-mouse secondary antibody (Thermo Fisher; 1:400) and an Alexa Fluor 488-conjugated anti-rabbit secondary antibody (Thermo Fisher; 1:400). ..

    Staining:

    Article Title: Self-Organized Vascularized Cardiac Microtissues Derived from Human iPS Cells Promote Myocardial Repair through Functional Host–Graft Vascular Integration
    Article Snippet: Visualization was carried out using the conventional HRP/DAB method with Avidin-biotin-peroxidase complex (ABC-Elite, Vector Laboratories) (1:100). .. Immunohistochemical fluorescence staining for STEM121 and von Willebrand factor (vWF) were conducted, using a mouse monoclonal STEM121 antibody specific for human cytoplasmic marker (#Y40410; Takara Bio Inc., Kusatsu, Japan) (1:400) and a rabbit polyclonal anti-vWF antibody (#ab6994; Abcam) (1:1000) was used as the primary antibody, followed by an Alexa Fluor 546-conjugated anti-mouse secondary antibody (Thermo Fisher; 1:400) and an Alexa Fluor 488-conjugated anti-rabbit secondary antibody (Thermo Fisher; 1:400). ..

    Marker:

    Article Title: Self-Organized Vascularized Cardiac Microtissues Derived from Human iPS Cells Promote Myocardial Repair through Functional Host–Graft Vascular Integration
    Article Snippet: Visualization was carried out using the conventional HRP/DAB method with Avidin-biotin-peroxidase complex (ABC-Elite, Vector Laboratories) (1:100). .. Immunohistochemical fluorescence staining for STEM121 and von Willebrand factor (vWF) were conducted, using a mouse monoclonal STEM121 antibody specific for human cytoplasmic marker (#Y40410; Takara Bio Inc., Kusatsu, Japan) (1:400) and a rabbit polyclonal anti-vWF antibody (#ab6994; Abcam) (1:1000) was used as the primary antibody, followed by an Alexa Fluor 546-conjugated anti-mouse secondary antibody (Thermo Fisher; 1:400) and an Alexa Fluor 488-conjugated anti-rabbit secondary antibody (Thermo Fisher; 1:400). ..

    Immunolabeling:

    Article Title: Transplantation of human neural stem cells repairs neural circuits and restores neurological function in the stroke-injured brain
    Article Snippet: Their brains were fixed in 2% paraformaldehyde with 3% saccharose for 30 minutes, dehydrated with 30% saccharose overnight, embedded in cryomatrix, and cut into 15-μm coronal slices using a microtome (CM-1950, Leica, Heidelberg, Germany). .. To identify whether the transplanted hNSCs survived after 12 weeks and to determine their differentiation status within the brain, the brain slices (every eighth sections) were immunolabeled using the following primary antibodies: Stem121 (mouse monoclonal antibody, 1:500, Takara, Kyoto, Japan, Cat# Y40410, RRID: AB_2801314) for human cell labeling to assess grafted human cell bodies and double-labeling for neural cell markers, including Tuj-1 (rabbit polyclonal antibody, 1:200, Abcam, Cat# ab18207, RRID: AB_444319) for immature and mature neurons; microtubule associated protein 2 (MAP2; chicken polyclonal antibody, 1:200, Abcam, Cat# ab5392, RRID: AB_2138153) for mature neurons; NG2 (rabbit polyclonal antibody, 1:300, Abcam, Cat# ab83178, RRID: AB_10672215) to label oligodendrocytes, and GFAP (rabbit monoclonal antibody, 1:200, Abcam, Cat# ab33922, RRID: AB_732571) to label astrocytes. .. Choline acetyltransferase (ChAT; rabbit monoclonal antibody, 1:200, Abcam, Cat# ab181023, RRID: AB_2687983) was used for staining mature excitatory motor neurons, and gamma-aminobutyric acid (GABA; rabbit polyclonal antibody, 1:200, Sigma-Aldrich, Cat# A2052, RRID: AB_477652) for staining GABAergic inhibitory neurons.

    Labeling:

    Article Title: Transplantation of human neural stem cells repairs neural circuits and restores neurological function in the stroke-injured brain
    Article Snippet: Their brains were fixed in 2% paraformaldehyde with 3% saccharose for 30 minutes, dehydrated with 30% saccharose overnight, embedded in cryomatrix, and cut into 15-μm coronal slices using a microtome (CM-1950, Leica, Heidelberg, Germany). .. To identify whether the transplanted hNSCs survived after 12 weeks and to determine their differentiation status within the brain, the brain slices (every eighth sections) were immunolabeled using the following primary antibodies: Stem121 (mouse monoclonal antibody, 1:500, Takara, Kyoto, Japan, Cat# Y40410, RRID: AB_2801314) for human cell labeling to assess grafted human cell bodies and double-labeling for neural cell markers, including Tuj-1 (rabbit polyclonal antibody, 1:200, Abcam, Cat# ab18207, RRID: AB_444319) for immature and mature neurons; microtubule associated protein 2 (MAP2; chicken polyclonal antibody, 1:200, Abcam, Cat# ab5392, RRID: AB_2138153) for mature neurons; NG2 (rabbit polyclonal antibody, 1:300, Abcam, Cat# ab83178, RRID: AB_10672215) to label oligodendrocytes, and GFAP (rabbit monoclonal antibody, 1:200, Abcam, Cat# ab33922, RRID: AB_732571) to label astrocytes. .. Choline acetyltransferase (ChAT; rabbit monoclonal antibody, 1:200, Abcam, Cat# ab181023, RRID: AB_2687983) was used for staining mature excitatory motor neurons, and gamma-aminobutyric acid (GABA; rabbit polyclonal antibody, 1:200, Sigma-Aldrich, Cat# A2052, RRID: AB_477652) for staining GABAergic inhibitory neurons.



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