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ssrna40 lyovec  (InvivoGen)


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    InvivoGen ssrna40 lyovec
    Ssrna40 Lyovec, supplied by InvivoGen, used in various techniques. Bioz Stars score: 95/100, based on 298 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ssrna40 lyovec/product/InvivoGen
    Average 95 stars, based on 298 article reviews
    ssrna40 lyovec - by Bioz Stars, 2026-02
    95/100 stars

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    (A) PCA plot for R2WT and R2KO macrophage cells and R2WT/R2KO exposed to <t>ssRNA40</t> (WT40/KO40, respectively) of mRNA sequencing. (B) Comparison of the distribution of differentially expressed transcripts (Cut-off: (|Log 2 FC|>1 and padj<0.01) between WT40 vs R2WT and KO40 vs R2KO. Kolmogorov-Smirnov test was used to do the statistical analysis. ****p < 0.0001. (C) Dotplot of KEGG pathway enrichment cluster (qvalue<0.05) of protein-coding genes for WT40, KO40 or R2KO versus R2WT. The genes with cut-off:|Log 2 FC|>2.32 and padj<0.01) were selected. (D, E and F) Go (Gene Ontology) analysis for unique WT40 up-expressed protein-coding genes (IR analysis). Topgo plot (D) of Biological Process (BP) analysis and dotplot of Cellular Component (CC) (E) and molecular function (MF) (F) analysis. The genes with cut-off:|Log 2 FC|>2.32 and padj<0.01) were selected.
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    (A) PCA plot for R2WT and R2KO macrophage cells and R2WT/R2KO exposed to ssRNA40 (WT40/KO40, respectively) of mRNA sequencing. (B) Comparison of the distribution of differentially expressed transcripts (Cut-off: (|Log 2 FC|>1 and padj<0.01) between WT40 vs R2WT and KO40 vs R2KO. Kolmogorov-Smirnov test was used to do the statistical analysis. ****p < 0.0001. (C) Dotplot of KEGG pathway enrichment cluster (qvalue<0.05) of protein-coding genes for WT40, KO40 or R2KO versus R2WT. The genes with cut-off:|Log 2 FC|>2.32 and padj<0.01) were selected. (D, E and F) Go (Gene Ontology) analysis for unique WT40 up-expressed protein-coding genes (IR analysis). Topgo plot (D) of Biological Process (BP) analysis and dotplot of Cellular Component (CC) (E) and molecular function (MF) (F) analysis. The genes with cut-off:|Log 2 FC|>2.32 and padj<0.01) were selected.

    Journal: bioRxiv

    Article Title: Human RNase 2 is essential for macrophage response to viral RNA

    doi: 10.64898/2025.12.03.691791

    Figure Lengend Snippet: (A) PCA plot for R2WT and R2KO macrophage cells and R2WT/R2KO exposed to ssRNA40 (WT40/KO40, respectively) of mRNA sequencing. (B) Comparison of the distribution of differentially expressed transcripts (Cut-off: (|Log 2 FC|>1 and padj<0.01) between WT40 vs R2WT and KO40 vs R2KO. Kolmogorov-Smirnov test was used to do the statistical analysis. ****p < 0.0001. (C) Dotplot of KEGG pathway enrichment cluster (qvalue<0.05) of protein-coding genes for WT40, KO40 or R2KO versus R2WT. The genes with cut-off:|Log 2 FC|>2.32 and padj<0.01) were selected. (D, E and F) Go (Gene Ontology) analysis for unique WT40 up-expressed protein-coding genes (IR analysis). Topgo plot (D) of Biological Process (BP) analysis and dotplot of Cellular Component (CC) (E) and molecular function (MF) (F) analysis. The genes with cut-off:|Log 2 FC|>2.32 and padj<0.01) were selected.

    Article Snippet: The ssRNA40 was complemented with LyoVecTM (InvivoGen) transfection reagent.

    Techniques: Sequencing, Comparison

    Parental tRNAs with significant coverage differences between R2WT and R2KO macrophage cells after 14h ssRNA40 exposure (WT40 and KO40, respectively) are depicted and the identified tDRs are marked in red. The possible cleavage sites were based on the 3′-terminal positions of the five prime fragments (green arrows) and the 5′ terminal positions of three prime fragments (blue arrows) according to the different coverage (Figure S11). The log 2 normalized read count for all the 4 group were plotted under the corresponding tRNA (tRAX output). Statistical analysis was done by ordinary one-way ANOVA with Tukey-test for multiple comparisons. p -value was present between groups. Only sequences with a log 2 FC > 1.0 and padj < 0.05 were included. The detailed of main fragments and their corresponding tDRs types are listed in Table S1.

    Journal: bioRxiv

    Article Title: Human RNase 2 is essential for macrophage response to viral RNA

    doi: 10.64898/2025.12.03.691791

    Figure Lengend Snippet: Parental tRNAs with significant coverage differences between R2WT and R2KO macrophage cells after 14h ssRNA40 exposure (WT40 and KO40, respectively) are depicted and the identified tDRs are marked in red. The possible cleavage sites were based on the 3′-terminal positions of the five prime fragments (green arrows) and the 5′ terminal positions of three prime fragments (blue arrows) according to the different coverage (Figure S11). The log 2 normalized read count for all the 4 group were plotted under the corresponding tRNA (tRAX output). Statistical analysis was done by ordinary one-way ANOVA with Tukey-test for multiple comparisons. p -value was present between groups. Only sequences with a log 2 FC > 1.0 and padj < 0.05 were included. The detailed of main fragments and their corresponding tDRs types are listed in Table S1.

    Article Snippet: The ssRNA40 was complemented with LyoVecTM (InvivoGen) transfection reagent.

    Techniques:

    The most significant changed genes associated to RNase 2 and ssRNA40 exposure by IR analysis are in black (cut-off: log 2 FC>5, padj<0.01) and grey (cut-off: log 2 FC>0, padj<0.01). The pathways where RNase 2 may be involved are indicated by red dashed lines overlapping with standard pathways of immune response to viral RNA exposure.

    Journal: bioRxiv

    Article Title: Human RNase 2 is essential for macrophage response to viral RNA

    doi: 10.64898/2025.12.03.691791

    Figure Lengend Snippet: The most significant changed genes associated to RNase 2 and ssRNA40 exposure by IR analysis are in black (cut-off: log 2 FC>5, padj<0.01) and grey (cut-off: log 2 FC>0, padj<0.01). The pathways where RNase 2 may be involved are indicated by red dashed lines overlapping with standard pathways of immune response to viral RNA exposure.

    Article Snippet: The ssRNA40 was complemented with LyoVecTM (InvivoGen) transfection reagent.

    Techniques: