Journal: Journal of Clinical Microbiology

Article Title: Comparative study of plasma microbial cell-free DNA sequencing to culture and polymerase chain reaction in pediatric community-acquired pneumonia with parapneumonic effusion or empyema

doi: 10.1128/jcm.01216-25

Figure Lengend Snippet: Theoretical impact analysis on time to pathogen detection for cCAP cases by testing modality (N = 48). Kaplan-Meier curves of time from hospital admission to pathogen detection based on the solid line: actual blood and pleural fluid culture testing. Dashed line: theoretical plasma microbial cell-free DNA sequencing if collected and sent for testing at the time of actual blood culture collection (or time of cCAP diagnosis if no blood culture was collected) with a turnaround time of either 48 or 72 h, dependent on collection time. Dotted line: theoretical pleural fluid PCRs if run at the time of pleural fluid drainage, with a turnaround time of 6 h for the on-demand S. aureus PCR and 24 h for batched group A Streptococcus and Streptococcus pneumoniae PCRs run daily. Patients were censored at hospital discharge if they had not achieved the outcome of interest (pathogen detected). Censoring is indicated by vertical tick marks.

Article Snippet: Based on typical CHCO and Karius laboratory protocols and timelines, we estimated a turnaround time of 6 h for the on-demand S. aureus PCR, 24 h for batched GAS and Spn PCRs run daily, and either 48 or 72 h for mcfDNA sequencing, dependent on time of collection and standard laboratory shipping times.

Techniques: Clinical Proteomics, DNA Sequencing, Biomarker Discovery

Journal: bioRxiv

Article Title: Second-order regulation: IFN-γ suppresses IL-17A-mediated neutrophilic inflammation

doi: 10.64898/2026.01.05.697792

Figure Lengend Snippet: A) Schematic of experimental model used. B) Spn serotype 3 CFU counts 24 hours post infection (hpi). Dotted line indicates the bacterial load in the challenge inoculum. Ordinary one-way ANOVA. C) Neutrophil numbers in BAL of naive and Spn experienced WT and IFN-γ KO mice 8 hpi. Lognormal ordinary one-way ANOVA. D) Graphical abstract illustrating our proposed working model of second-order cross-regulation between type 1 and type 3 inflammatory cytokines within non-lymphoid tissues. p value: ns≤ 0.1234, *≤ 0.0332, **≤ 0.0021, ***≤ 0.0002, ****≤ 0.0001. All data have n≥3 mice, 2 experiments, mean ± SEM.

Article Snippet: On day 36, the mice were intratracheally challenged with 1 × 10 6 CFU of serotype 3 Spn (Sp3, ATCC 6303), suspended in 100μL of sterile PBS.

Techniques: Infection