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Image Search Results
Journal: bioRxiv
Article Title: Altered X-chromosome inactivation predisposes to autoimmune manifestations in mice
doi: 10.1101/2023.04.20.537662
Figure Lengend Snippet: a. Spleen weight of wild-type (WT) and Ftx KO females at 3-months, 1-year and 2-years of age. Median values are shown. ( t-test , * p -values < 0.05). Underneath, representative images of WT and Ftx KO spleens from 3-month, 1-year and 2-year-old females. b. Representative images of hematoxylin-eosin staining on sections of spleens from 1-year-old WT and Ftx KO females. Scale bar; 100 μm. c. Representative flow cytometry analysis of splenic myeloid dendritic cells (m-DC) in WT and Ftx KO 1-year-old females. On the right, percentages of splenic m-DC (CD11b + CD11c + ) and splenic plasmacytoid dendritic cells (p-DC) (CD11c + B220 + SiglecH + ) in leucocytes. Each triangle represents a mouse. Median values are shown. ( t-test , * p -values < 0.05). d. Representative flow cytometry analysis of spontaneously activated B cells (B220 + CD69 + ) (upper panels) or of spontaneously activated T cells (CD4 + CD69 + ) (lower panels) in spleen from 1-year-old WT and Ftx KO females. Percentages in leucocytes are shown on the graphs beneath. Each triangle represents a mouse. Median values are shown. ( t-test , * p -values < 0.05). e. IgG2a natural antibody levels in sera of 1-year- and 2-year-old WT or Ftx KO females measured by ELISA. Each triangle represents a mouse. Median values are shown. ( t-test , * p -values < 0.05). f. Representative flow cytometry analysis of (B220 + CD138 + ) plasma cells in the spleen of 1-year-old WT and Ftx KO females. Percentages in leucocytes are shown on the graphs beneath. Each triangle represents a mouse. Median values are shown. ( t-test , * p -values < 0.05). g. Representative flow cytometry analysis of (CD19 + B220 lo CD5 + ) natural antibody producing B1a in the peritoneal cavity (PerC) of 1-year-old WT and Ftx KO females. Percentages in leucocytes are shown on the graphs beneath. Each triangle represents a mouse. Median values are shown. ( t-test , * p -values < 0.05). h. Cytokines levels in the blood analysed with CBA assays on sera from 3-month-, 1-year-, or 2-year-old WT and Ftx KO females. Each triangle represents a mouse. Median values are shown. ( t-test , * p -values < 0.05). i. Representative flow cytometry analysis of monocyte populations including non-classical (CD11b hi CD43 lo Ly6C + ) scavenger monocytes in the spleen of 1-year-old WT and Ftx KO females. Percentages in leucocytes are shown on the graphs beneath. Each triangle represents a mouse. Median values are shown. ( t-test , * p -values < 0.05).
Article Snippet: Bone marrow, spleen, blood and peritoneal cavity cells were stained using the following antibodies: CD3 PerCP-Vio770 (130-119-656, Miltenyi Biotec), CD4-APC (130-123-207, Miltenyi Biotec), CD5-APC-Vio770 (130-120-165, Miltenyi Biotec), CD8-FITC (130-118-468, Miltenyi Biotec), CD11b APC (553312, BD Pharmingen), CD11c PE-Vio770 (130-110-840, Miltenyi Biotec), CD19-FITC (557398, BD Pharmingen), CD21-APC-Vio770 (130-111-733, Miltenyi Biotec), CD23-PE-Vio770 (130-118-764, Miltenyi Biotec), CD38-PE (130-123-571, Miltenyi Biotec),
Techniques: Staining, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Clinical Proteomics
Journal: Cell
Article Title: Spatial proteogenomics reveals distinct and evolutionarily conserved hepatic macrophage niches
doi: 10.1016/j.cell.2021.12.018
Figure Lengend Snippet:
Article Snippet:
Techniques: Purification, Recombinant, Staining, cDNA Synthesis, Gene Expression, Software, Microscopy
Journal: Cell reports
Article Title: SOX17 integrates HOXA and arterial programs in hemogenic endothelium to drive definitive lympho-myeloid hematopoiesis
doi: 10.1016/j.celrep.2021.108758
Figure Lengend Snippet: (A) Schematic diagram of hematopoietic development and in defined conditions. D, day of differentiation. (B) HB-CFC potential of wild-type or SOX17 −/− H9 cells (means ± SDs, for 2 independent experiments performed in duplicate). **p < 0.01, t test. (C) Flow cytometric analysis of day 4 HE. (D and E) Flow cytometric analysis of day 5 HE. Graphs show the percentages and total number of cells generated from 10 4 hESCs (means ± SDs, n = 3 experiments). **p < 0.01 and ***p < 0.001, t test. (F) Schematic diagram of experiments. Wild and SOX17 −/− cells were purified using CD31 magnetic-activated cell sorting (MACS) on day 4 and plated on OP9 or OP9-DLL4 for 5 days. (G) Hematopoietic colony-forming potential of day 4 HE after 5 days of culture on OP9 or OP9-DLL4 (means ± SDs, n = 2 experiments). ****p < 0.0001, 2-way ANOVA, Tukey’s multiple comparisons test. (H) Flow cytometric analysis of T cell differentiation. (I) Graphs show the total number of T cells generated from 10 4 CD43 + cells (means ± SDs, n = 3 experiments). ****p < 0.0001, 2-way ANOVA, Tukey’s multiple comparisons test. See also .
Article Snippet:
Techniques: Generated, Purification, FACS, Cell Differentiation
Journal: Cell reports
Article Title: SOX17 integrates HOXA and arterial programs in hemogenic endothelium to drive definitive lympho-myeloid hematopoiesis
doi: 10.1016/j.celrep.2021.108758
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet:
Techniques: Recombinant, Chromatin Immunoprecipitation, Control, Expressing, Plasmid Preparation, Software