lentiviral spi1 overexpression virus (Genechem)
86
Structured Review
Genechem
lentiviral spi1 overexpression virus
Lentiviral Spi1 Overexpression Virus, supplied by Genechem, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/spi1/hairpin+rna+short/pmc13276326-9-0-5
Average 86 stars, based on 1 article reviews
Lentiviral Spi1 Overexpression Virus, supplied by Genechem, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/spi1/hairpin+rna+short/pmc13276326-9-0-5
Average 86 stars, based on 1 article reviews
lentiviral spi1 overexpression virus - by Bioz Stars,
2026-09
86/100 stars
Images
1) Product Images from "Hyaluronic acid and folic acid-modified mesoporous silica nanoparticles delivering sulforaphane suppress NSCLC via SPI1/miR-616-5p axis"
Article Title: Hyaluronic acid and folic acid-modified mesoporous silica nanoparticles delivering sulforaphane suppress NSCLC via SPI1/miR-616-5p axis
Journal: iScience
doi: 10.1016/j.isci.2026.116293
Figure Legend Snippet: SPI1 regulates NSCLC cell viability and proliferation (A–C) Western blot analysis of transfection efficiency for SPI1 overexpression and knockdown ( n = 3, n represents biological replicates). (D) CCK-8 assay assessing the effects of SPI1 overexpression and knockdown on NSCLC cell viability ( n = 6, n represents biological replicates). (E) Colony formation assay evaluating the impact of SPI1 overexpression and knockdown on NSCLC cell proliferation ( n = 3, n represents biological replicates). Data are represented as mean ± SD. p values are based on a one-way ANOVA test. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.
Techniques Used: Western Blot, Transfection, Over Expression, Knockdown, CCK-8 Assay, Colony Assay
Figure Legend Snippet: SPI1 regulates NSCLC cell migration, invasion, and EMT (A) Transwell assay assessing the effects of SPI1 on cell migration and invasion ( n = 3, n represents biological replicates), scale bars, 200 μm. (B) Western blot analysis of the impact of SPI1 on the expression levels of EMT-related proteins (N-cadherin, E-cadherin, and vimentin, n = 3, n represents biological replicates). Data are represented as mean ± SD. p values are based on a one-way ANOVA test. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. Ⅰ, N-cadherin. Ⅱ, E-cadherin. Ⅲ, vimentin. Ⅳ, GAPDH.
Techniques Used: Migration, Transwell Assay, Western Blot, Expressing
Figure Legend Snippet: SPI1 promotes NSCLC cell invasion and migration by transcriptionally regulating miR-616-5p (A and B) RT-PCR analysis of miR-616-5p expression following SPI1 overexpression or knockdown ( n = 3, n represents biological replicates). (C–E) Transwell assay evaluating cell migration and invasion ( n = 3, n represents biological replicates), scale bars, 200 μm. Data are represented as mean ± SD. p values are based on a one-way ANOVA test. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.
Techniques Used: Migration, Reverse Transcription Polymerase Chain Reaction, Expressing, Over Expression, Knockdown, Transwell Assay
Figure Legend Snippet: SF downregulates miR-616-5p activity by directly binding to and inhibiting SPI1 (A) Molecular docking analysis of SF with SPI1. (B) SPRi binding curve of SF to SPI1. (C and D) Western blot analysis of SPI1 expression in cells ( n = 3, n represents biological replicates). (E) RT-PCR analysis of miR-616-5p expression in cells ( n = 3, n represents biological replicates). Data are represented as mean ± SD. p values are based on a one-way ANOVA test. ∗ p < 0.05, ∗∗ p < 0.01.
Techniques Used: Activity Assay, Binding Assay, Western Blot, Expressing, Reverse Transcription Polymerase Chain Reaction
Figure Legend Snippet: In vivo targeting and anti-NSCLC efficacy of MSNs@SF-HA-FA (A) In vivo fluorescence imaging analysis ( n = 5, n represents the number of mice). (B) Ex vivo fluorescence imaging of major organs ( n = 5, n represents the number of mice). (C) Images of lung tumor tissues from mice ( n = 5, n represents the number of mice). (D) H&E-stained images and immunohistochemical analysis of SPI1 expression in xenograft tumors ( n = 5, n represents the number of mice), scale bars, 100 μm.
Techniques Used: In Vivo, Fluorescence, Imaging, Ex Vivo, Staining, Immunohistochemical staining, Expressing
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