Review

sox13 (Proteintech)

Proteintech is a verified supplier

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Proteintech sox13

POU3F2 activates expression of <t>SOX13</t> and ADNP to regulate canonical Wnt signalling in NPCs. ( A ) Potential direct effectors of POU3F2 were prioritized if they belonged to the TCF-dependent signalling in response to Wnt Reactome pathway (R-HSA-201681). ( B ) Quantification of ADNP protein expression measured via western blotting in POU3F2 WT and POU3F2 MUT NPCs, mean ± SEM ( n = 4 differentiations). Paired t -test, * P < 0.05. ( C ) Quantification of SOX13 protein expression measured via western blotting in POU3F2 WT and POU3F2 MUT NPCs, mean ± SEM ( n = 5 differentiations). Paired t -test, ** P < 0.01. ( D ) Schematic of experimental design for overexpression of a gene of interest followed by the SUPERTOPFLASH canonical Wnt reporter assay. ( E ) Baseline canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or ADNP, mean ± SEM ( n = 3 differentiations). Mixed-effects model with post hoc Sidak's test, ns = not significant. ( F ) Activated canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or ADNP, mean ± SEM ( n = 3 differentiations). Mixed-effects model with post hoc Sidak's test, * P < 0.05, ** P < 0.01. ( G ) Baseline canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or SOX13, mean ± SEM ( n = 3 differentiations). GFP control data represented here is included in . Mixed-effects model with post hoc Sidak's test, * P < 0.05, ** P < 0.01. ( H ) Activated canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or SOX13, mean ± SEM ( n = 3 differentiations). GFP control data represented here is included in . Mixed-effects model with post hoc Sidak's test, * P < 0.05, *** P < 0.001. ( I ) Genomic structure of SOX13 . Box above gene indicates relative position of gRNA target sequence. Western blot validation of SOX13 knockdown (GAPDH = loading control). ( J ) Gene ontology pathway enrichment results of differential gene expression in SOX13 MUT NPCs, separated by directionality of log 2 fold change. ( K ) GSEA of Wnt-activated targets and G2/M progenitor markers in SOX13 MUT versus SOX13 WT differential expression. ( L ) Model of POU3F2-mediated regulation of canonical Wnt signalling and progenitor expansion via transcription of ADNP and SOX13 . Schematics created in BioRender. Benoit, C. (2025) https://BioRender.com/35q22ly , https://BioRender.com/baxexcu . gRNA = guide RNA; GSEA = gene set enrichment analysis; MUT = mutant; NPC = neural progenitor cell; SEM = standard error of the mean; WT = wild-type.

Sox13, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sox13/SOX13+Antibody/pmc12677912-65-63-64
Average 93 stars, based on 7 article reviews

sox13 - by Bioz Stars, 2026-09

93/100 stars

Images

1) Product Images from "POU3F2 regulates canonical Wnt signalling via SOX13 and ADNP to expand the neural progenitor population"

Article Title: POU3F2 regulates canonical Wnt signalling via SOX13 and ADNP to expand the neural progenitor population

Journal: Brain

doi: 10.1093/brain/awaf221

POU3F2 activates expression of SOX13 and ADNP to regulate canonical Wnt signalling in NPCs. ( A ) Potential direct effectors of POU3F2 were prioritized if they belonged to the TCF-dependent signalling in response to Wnt Reactome pathway (R-HSA-201681). ( B ) Quantification of ADNP protein expression measured via western blotting in POU3F2 WT and POU3F2 MUT NPCs, mean ± SEM ( n = 4 differentiations). Paired t -test, * P < 0.05. ( C ) Quantification of SOX13 protein expression measured via western blotting in POU3F2 WT and POU3F2 MUT NPCs, mean ± SEM ( n = 5 differentiations). Paired t -test, ** P < 0.01. ( D ) Schematic of experimental design for overexpression of a gene of interest followed by the SUPERTOPFLASH canonical Wnt reporter assay. ( E ) Baseline canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or ADNP, mean ± SEM ( n = 3 differentiations). Mixed-effects model with post hoc Sidak's test, ns = not significant. ( F ) Activated canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or ADNP, mean ± SEM ( n = 3 differentiations). Mixed-effects model with post hoc Sidak's test, * P < 0.05, ** P < 0.01. ( G ) Baseline canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or SOX13, mean ± SEM ( n = 3 differentiations). GFP control data represented here is included in . Mixed-effects model with post hoc Sidak's test, * P < 0.05, ** P < 0.01. ( H ) Activated canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or SOX13, mean ± SEM ( n = 3 differentiations). GFP control data represented here is included in . Mixed-effects model with post hoc Sidak's test, * P < 0.05, *** P < 0.001. ( I ) Genomic structure of SOX13 . Box above gene indicates relative position of gRNA target sequence. Western blot validation of SOX13 knockdown (GAPDH = loading control). ( J ) Gene ontology pathway enrichment results of differential gene expression in SOX13 MUT NPCs, separated by directionality of log 2 fold change. ( K ) GSEA of Wnt-activated targets and G2/M progenitor markers in SOX13 MUT versus SOX13 WT differential expression. ( L ) Model of POU3F2-mediated regulation of canonical Wnt signalling and progenitor expansion via transcription of ADNP and SOX13 . Schematics created in BioRender. Benoit, C. (2025) https://BioRender.com/35q22ly , https://BioRender.com/baxexcu . gRNA = guide RNA; GSEA = gene set enrichment analysis; MUT = mutant; NPC = neural progenitor cell; SEM = standard error of the mean; WT = wild-type.

Figure Legend Snippet: POU3F2 activates expression of SOX13 and ADNP to regulate canonical Wnt signalling in NPCs. ( A ) Potential direct effectors of POU3F2 were prioritized if they belonged to the TCF-dependent signalling in response to Wnt Reactome pathway (R-HSA-201681). ( B ) Quantification of ADNP protein expression measured via western blotting in POU3F2 WT and POU3F2 MUT NPCs, mean ± SEM ( n = 4 differentiations). Paired t -test, * P < 0.05. ( C ) Quantification of SOX13 protein expression measured via western blotting in POU3F2 WT and POU3F2 MUT NPCs, mean ± SEM ( n = 5 differentiations). Paired t -test, ** P < 0.01. ( D ) Schematic of experimental design for overexpression of a gene of interest followed by the SUPERTOPFLASH canonical Wnt reporter assay. ( E ) Baseline canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or ADNP, mean ± SEM ( n = 3 differentiations). Mixed-effects model with post hoc Sidak's test, ns = not significant. ( F ) Activated canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or ADNP, mean ± SEM ( n = 3 differentiations). Mixed-effects model with post hoc Sidak's test, * P < 0.05, ** P < 0.01. ( G ) Baseline canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or SOX13, mean ± SEM ( n = 3 differentiations). GFP control data represented here is included in . Mixed-effects model with post hoc Sidak's test, * P < 0.05, ** P < 0.01. ( H ) Activated canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or SOX13, mean ± SEM ( n = 3 differentiations). GFP control data represented here is included in . Mixed-effects model with post hoc Sidak's test, * P < 0.05, *** P < 0.001. ( I ) Genomic structure of SOX13 . Box above gene indicates relative position of gRNA target sequence. Western blot validation of SOX13 knockdown (GAPDH = loading control). ( J ) Gene ontology pathway enrichment results of differential gene expression in SOX13 MUT NPCs, separated by directionality of log 2 fold change. ( K ) GSEA of Wnt-activated targets and G2/M progenitor markers in SOX13 MUT versus SOX13 WT differential expression. ( L ) Model of POU3F2-mediated regulation of canonical Wnt signalling and progenitor expansion via transcription of ADNP and SOX13 . Schematics created in BioRender. Benoit, C. (2025) https://BioRender.com/35q22ly , https://BioRender.com/baxexcu . gRNA = guide RNA; GSEA = gene set enrichment analysis; MUT = mutant; NPC = neural progenitor cell; SEM = standard error of the mean; WT = wild-type.

Techniques Used: Expressing, Western Blot, Over Expression, Reporter Assay, Negative Control, Control, Sequencing, Biomarker Discovery, Knockdown, Gene Expression, Quantitative Proteomics, Mutagenesis

Incubation:

Article Title: POU3F2 regulates canonical Wnt signalling via SOX13 and ADNP to expand the neural progenitor population
Article Snippet: Cells were fixed in 4% paraformaldehyde for 15 min at room temperature then blocked in 2% donkey serum (Jackson ImmunoResearch) and 0.2% Triton-X-100 (Sigma) for 1 h at room temperature. .. Cells were incubated with primary antibody diluted in blocking buffer overnight at 4°C: POU3F2 (CST, Cat. No. 12137S, 1:100), Nestin (Novus Biologicals, Cat. No. MAB1259, 1:500), PAX6 (Biolegend, Cat. No. 901301, 1:400), SOX2 (R&D Systems, Cat. No. AF2018, 1:500), MAP2 (Abcam, Cat. No. ab5392, 1:500), CD44 (Abcam, Cat. No. ab254530, 1:500), ANXA1 (Abcam, Cat. No. 214486, 1:100), BLBP (R&D Systems, Cat. No. AF3166, 1:100), ADNP (Abcam, Cat. No. ab300114, 1:100), SOX13 (Proteintech, Cat. No. 18902-1, 1:100), β-catenin (CST, Cat. No. 8480S, 1:100). ..

Article Title: FUS/circ_002136/miR-138-5p/SOX13 feedback loop regulates angiogenesis in Glioma
Article Snippet: .. The membranes were subsequently incubated with primary antibodies as follows: FUS (1:2000; Proteintech, Chicago, IL, USA), SOX13 (1:500; Proteintech), SPON2 (1:1000; Affinity, Cincinnati, OH, USA), and GAPDH (1:10,000; Proteintech) at 4 °C overnight. .. The membranes were washed and incubated with HRP-conjugated secondary antibodies (Santa Cruz Biotechnology, Santa Cruz, CA, USA), diluted at 1:5000 at room temperature for two hours.

Blocking Assay:

Article Title: POU3F2 regulates canonical Wnt signalling via SOX13 and ADNP to expand the neural progenitor population
Article Snippet: Proteins were transferred to nitrocellulose membranes in 20% methanol Tris-glycine transfer buffer at 400 mA for 2 h, then blocked with Odyssey blocking buffer (LI-COR) for 1 h at room temperature. .. Primary antibodies diluted in blocking buffer were applied overnight at 4°C: POU3F2 (CST, Cat. No. 12137S, 1:1000), GAPDH (Proteintech, Cat. No. 60004-1-Ig, 1:10 000), β-catenin (CST, Cat. No. 8480S, 1:1000), Nestin (Novus Biologicals, Cat. No. MAB1259, 1:1000), TUJ1 (Novus Biologicals, Cat. No. NB100-1612, 1:1000), POU3F2 (Santa Cruz, Cat. No. sc-393324, 1:1000), HA-Tag (CST, Cat. No. C29F4, 1:500), ADNP (Abcam, Cat. No. ab300114, 1:500), SOX13 (Proteintech, Cat. No. 18902-1, 1:1000). .. Blots were incubated with LI-COR secondary antibodies (1:10 000 dilution) for 1 h at room temperature.

Immunohistochemistry:

Article Title: Targeting SOX13 inhibits assembly of respiratory chain supercomplexes to overcome ferroptosis resistance in gastric cancer
Article Snippet: .. Antibody IHC WB CHIP Specificity Company (catalog number) SOX13 1:100 1:1000 1:50 Rabbit polyclonal proteintech (18902-1-AP) COX7A2L/SCAF1 1:100 1:1000 / Rabbit Polyclonal proteintech (11416-1-AP) NADK / 1:1000 / Rabbit polyclonal Abcam (ab233261) NADK2 / 1:1000 / Rabbit monoclonal Abcam (ab181028) ACSL4 / 1:1000 / Rabbit monoclonal Abcam (ab155282) SLC7A11 / 1:1000 / Rabbit monoclonal Abcam (ab175186) GPX4 / 1:1000 / Rabbit monoclonal Abcam (ab125066) E-cadherin / 1:800 / Mouse monoclonal Abcam (ab1416) Vimentin / 1:1000 / Rabbit monoclonal Abcam (ab92547) SDHA / 1:1000 / Mouse monoclonal Abcam (ab14715) MTCO1 / 1:1000 / Mouse monoclonal Abcam (ab14705) NDUFS2 / 1:1000 / Mouse monoclonal Abcam (ab110249) UQCRC2 / 1:1000 / Mouse monoclonal Abcam (ab14745) Trim25 / 1:1000 / Rabbit monoclonal Abcam (ab167154) FBXO28 / 1:800 Rabbit polyclonal Abcam (ab154068) TRAF2 / 1:1000 Rabbit monoclonal Abcam (ab126758) β-Actin / 1:2000 / Rabbit polyclonal Abcam (ab8227) Supplementary Table S5. ..

Western Blot:

Image Search Results

A Combinatorial analysis of genes differentially expressed between parental and ferroptosis-resistant cells and genes that are strongly correlated with sensitivity to ferroptosis inducers in CTRP. Six common genes were identified, among which was SOX13. B qRT–PCR analysis of SOX13 transcript levels in resistant cell lines ( n = 3 independent experiments). RSL3 resis , RSL3 resistance; Erastin resis , Erastin resistance. Data are presented as mean values ± SD. C Box-and-whisker plots show 5st and 95th percentile outlier compounds (red dots) where SOX13 expression levels are correlated with cell line sensitivity to the compounds in CTRP – . Plotted values are Z-scored Pearson’s correlation coefficients. Line, median; box, 10th–90th percentiles. A positive z-score means that high expression is correlated with high resistance to compounds, and vice versa. D SOX13 was determined by immunoblotting assay and normalized to β-actin ( n = 3 independent experiments). E Effect of SOX13 downregulation and SOX13 re-expression on Erastin (2 μM) or RSL3 (0.5 μM) sensitivity in the absence or presence of Z-VAD-FMK (10 μM), NSA (1 μM), or Fer-1 (1 μM) in parental versus resistant cells. The cells were treated with FINs for 24 h ( n = 3 independent experiments). Cell viability was evaluated with a CellTiter-Glo luminescent cell viability assay ( n = 3 independent experiments). Data are presented as mean values ± SD. Statistical significance in ( B ) and ( E ) is determined by two-tailed unpaired t test. Source data are provided as a file.

Journal: Nature Communications

Article Title: Targeting SOX13 inhibits assembly of respiratory chain supercomplexes to overcome ferroptosis resistance in gastric cancer

doi: 10.1038/s41467-024-48307-z

Figure Lengend Snippet: A Combinatorial analysis of genes differentially expressed between parental and ferroptosis-resistant cells and genes that are strongly correlated with sensitivity to ferroptosis inducers in CTRP. Six common genes were identified, among which was SOX13. B qRT–PCR analysis of SOX13 transcript levels in resistant cell lines ( n = 3 independent experiments). RSL3 resis , RSL3 resistance; Erastin resis , Erastin resistance. Data are presented as mean values ± SD. C Box-and-whisker plots show 5st and 95th percentile outlier compounds (red dots) where SOX13 expression levels are correlated with cell line sensitivity to the compounds in CTRP – . Plotted values are Z-scored Pearson’s correlation coefficients. Line, median; box, 10th–90th percentiles. A positive z-score means that high expression is correlated with high resistance to compounds, and vice versa. D SOX13 was determined by immunoblotting assay and normalized to β-actin ( n = 3 independent experiments). E Effect of SOX13 downregulation and SOX13 re-expression on Erastin (2 μM) or RSL3 (0.5 μM) sensitivity in the absence or presence of Z-VAD-FMK (10 μM), NSA (1 μM), or Fer-1 (1 μM) in parental versus resistant cells. The cells were treated with FINs for 24 h ( n = 3 independent experiments). Cell viability was evaluated with a CellTiter-Glo luminescent cell viability assay ( n = 3 independent experiments). Data are presented as mean values ± SD. Statistical significance in ( B ) and ( E ) is determined by two-tailed unpaired t test. Source data are provided as a file.

Article Snippet: Initially, purified SOX13 protein (0.17 mg/mL) was dissolved in PBS and then immobilized onto the CM5 chip (GE Healthcare, USA).

Techniques: Quantitative RT-PCR, Whisker Assay, Expressing, Western Blot, Cell Viability Assay, Two Tailed Test

A , B Effect of SOX13 downregulation and SOX13 re-expression on Erastin (2 μM) or RSL3 (0.5 μM) sensitivity in the absence or presence of Fer-1 (1 μM) in resistant cells. The cells were treated with FINs for 24 h. A Intracellular MDA was assayed with ELISA ( n = 3 independent experiments). Data are presented as mean values ± SD. B Lipid peroxidation was determined with a lipid peroxidation C11-BODIPY assay in SNU-668 Erastin resis cells ( n = 3 independent experiments), and a representative flow cytometry histogram plot is presented. Data are presented as mean values ± SD. Statistical significance in ( A ) and ( B ) is determined by two-tailed unpaired t test. Source data are provided as a file.

Journal: Nature Communications

Article Title: Targeting SOX13 inhibits assembly of respiratory chain supercomplexes to overcome ferroptosis resistance in gastric cancer

doi: 10.1038/s41467-024-48307-z

Figure Lengend Snippet: A , B Effect of SOX13 downregulation and SOX13 re-expression on Erastin (2 μM) or RSL3 (0.5 μM) sensitivity in the absence or presence of Fer-1 (1 μM) in resistant cells. The cells were treated with FINs for 24 h. A Intracellular MDA was assayed with ELISA ( n = 3 independent experiments). Data are presented as mean values ± SD. B Lipid peroxidation was determined with a lipid peroxidation C11-BODIPY assay in SNU-668 Erastin resis cells ( n = 3 independent experiments), and a representative flow cytometry histogram plot is presented. Data are presented as mean values ± SD. Statistical significance in ( A ) and ( B ) is determined by two-tailed unpaired t test. Source data are provided as a file.

Article Snippet: Initially, purified SOX13 protein (0.17 mg/mL) was dissolved in PBS and then immobilized onto the CM5 chip (GE Healthcare, USA).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Two Tailed Test

A , B Effect of exogenous expression of SOX13 on sensitivity of parental SNU-668 cells (Left) or SNU-484 cells (Right) to the ferroptosis inducers RSL3 or Erastin. Cells were treated with lentivirus harboring the human SOX13 sequence or the empty vector, or in combination with various concentrations of Erastin (Left panel) or RSL3 (Right panel) for 24 h. The heatmap shows altered cell viability. B SNU-668 Cells were treated with lentivirus harboring the human SOX13 sequence or the empty vector, or in combination with Erastin (2 μM, Upper panel) or RSL3 (0.5 μM, Lower panel) for 24 h. Lipid peroxidation was determined using a lipid peroxidation C11-BODIPY assay ( n = 3 independent experiments), and representative flow cytometry histogram plot is presented. Data are presented as mean values ± SD. (C-G) SNU-668-SOX13-CDX tumors and SNU-668-NC-CDX tumors were treated with cisplatin and IKE. Representative images of tumors formed ( C ), tumor growth curves ( D ), tumor weights ( E ), T/C ratio ( F ) and PTGS2 ( G ) expression analysis are shown. Treatment with IKE (20 mg/kg, i.p., once daily), cisplatin (4 mg/kg, i.p., once weekly) or PBS (100 µl, i.p., once daily) started on Day 7 and lasted for 3 consecutive weeks ( n = 4 mice per group). T/C% = T RTV /C RTV × 100%; T RTV relative tumor volume after treatment; C RTV relative tumor volume of control group. Data are presented as mean values ± SD. H SOX13/SCAF1 expression in SNU-668 cell-derived xenografts was determined by immunoblotting and normalized to β-actin (Representative plot of experiments repeated in triplicate). C control, P cisplatin. Randomly selected two tumor samples per group are shown. Statistical significance in ( B ) and ( D , E , G ) is determined by two-tailed unpaired t test. Source data are provided as a file.

Journal: Nature Communications

Article Title: Targeting SOX13 inhibits assembly of respiratory chain supercomplexes to overcome ferroptosis resistance in gastric cancer

doi: 10.1038/s41467-024-48307-z

Figure Lengend Snippet: A , B Effect of exogenous expression of SOX13 on sensitivity of parental SNU-668 cells (Left) or SNU-484 cells (Right) to the ferroptosis inducers RSL3 or Erastin. Cells were treated with lentivirus harboring the human SOX13 sequence or the empty vector, or in combination with various concentrations of Erastin (Left panel) or RSL3 (Right panel) for 24 h. The heatmap shows altered cell viability. B SNU-668 Cells were treated with lentivirus harboring the human SOX13 sequence or the empty vector, or in combination with Erastin (2 μM, Upper panel) or RSL3 (0.5 μM, Lower panel) for 24 h. Lipid peroxidation was determined using a lipid peroxidation C11-BODIPY assay ( n = 3 independent experiments), and representative flow cytometry histogram plot is presented. Data are presented as mean values ± SD. (C-G) SNU-668-SOX13-CDX tumors and SNU-668-NC-CDX tumors were treated with cisplatin and IKE. Representative images of tumors formed ( C ), tumor growth curves ( D ), tumor weights ( E ), T/C ratio ( F ) and PTGS2 ( G ) expression analysis are shown. Treatment with IKE (20 mg/kg, i.p., once daily), cisplatin (4 mg/kg, i.p., once weekly) or PBS (100 µl, i.p., once daily) started on Day 7 and lasted for 3 consecutive weeks ( n = 4 mice per group). T/C% = T RTV /C RTV × 100%; T RTV relative tumor volume after treatment; C RTV relative tumor volume of control group. Data are presented as mean values ± SD. H SOX13/SCAF1 expression in SNU-668 cell-derived xenografts was determined by immunoblotting and normalized to β-actin (Representative plot of experiments repeated in triplicate). C control, P cisplatin. Randomly selected two tumor samples per group are shown. Statistical significance in ( B ) and ( D , E , G ) is determined by two-tailed unpaired t test. Source data are provided as a file.

Article Snippet: Initially, purified SOX13 protein (0.17 mg/mL) was dissolved in PBS and then immobilized onto the CM5 chip (GE Healthcare, USA).

Techniques: Expressing, Sequencing, Plasmid Preparation, Flow Cytometry, Derivative Assay, Western Blot, Two Tailed Test

A Combinatorial analysis of genes differentially expressed between ferroptosis-sensitive and ferroptosis-resistant cells, genes dysregulated in response to SOX13 downregulation in Erastin resis SNU-668 cells, and the genes with SOX13-bound transcription sites characterized by ChIP-seq. The core common gene SCAF1 was identified. B Metabolite set enrichment analysis (MSEA) was used to determine the top ten metabolic pathways enriched in SOX13-downregulated Erastin resis SNU-668 cells. C mRNA and protein levels of SCAF1 in resistant and parental SNU-668 and SNU-484 cell lines ( n = 3 independent experiments). Data are presented as mean values ± SD. D Effect of SOX13 knockdown on SCAF1 protein abundance in resistant cells (Representative blot of three experimental replicates, quantification shows three independent experiments). Data are presented as mean values ± SD. E Effect of SOX13 overexpression on SCAF1 protein abundance in parental cells ( n = 3 independent experiments). Data are presented as mean values ± SD. F Luciferase assay of GC cells cotransfected with firefly luciferase constructs containing the SCAF1 promoter and pCMV-SOX13 ( n = 3 independent experiments). Data are presented as mean values ± SD. G Luciferase assay of GC cells cotransfected with firefly luciferase constructs containing a series of SOX13 promoter deletion mutants and pCMV-SOX13 ( n = 3 independent experiments). Data are presented as mean values ± SD. H ChIP-qPCR analysis further verified that SOX13 directly accumulates at SCAF1 promoter regions in GC cells with SOX13 upregulation and in Erastin resis SNU-668 cells ( n = 3 independent experiments). Data are presented as mean values ± SD. Statistical significance in ( B – H ) is determined by two-tailed unpaired t -test. Source data are provided as a file.

Journal: Nature Communications

Article Title: Targeting SOX13 inhibits assembly of respiratory chain supercomplexes to overcome ferroptosis resistance in gastric cancer

doi: 10.1038/s41467-024-48307-z

Figure Lengend Snippet: A Combinatorial analysis of genes differentially expressed between ferroptosis-sensitive and ferroptosis-resistant cells, genes dysregulated in response to SOX13 downregulation in Erastin resis SNU-668 cells, and the genes with SOX13-bound transcription sites characterized by ChIP-seq. The core common gene SCAF1 was identified. B Metabolite set enrichment analysis (MSEA) was used to determine the top ten metabolic pathways enriched in SOX13-downregulated Erastin resis SNU-668 cells. C mRNA and protein levels of SCAF1 in resistant and parental SNU-668 and SNU-484 cell lines ( n = 3 independent experiments). Data are presented as mean values ± SD. D Effect of SOX13 knockdown on SCAF1 protein abundance in resistant cells (Representative blot of three experimental replicates, quantification shows three independent experiments). Data are presented as mean values ± SD. E Effect of SOX13 overexpression on SCAF1 protein abundance in parental cells ( n = 3 independent experiments). Data are presented as mean values ± SD. F Luciferase assay of GC cells cotransfected with firefly luciferase constructs containing the SCAF1 promoter and pCMV-SOX13 ( n = 3 independent experiments). Data are presented as mean values ± SD. G Luciferase assay of GC cells cotransfected with firefly luciferase constructs containing a series of SOX13 promoter deletion mutants and pCMV-SOX13 ( n = 3 independent experiments). Data are presented as mean values ± SD. H ChIP-qPCR analysis further verified that SOX13 directly accumulates at SCAF1 promoter regions in GC cells with SOX13 upregulation and in Erastin resis SNU-668 cells ( n = 3 independent experiments). Data are presented as mean values ± SD. Statistical significance in ( B – H ) is determined by two-tailed unpaired t -test. Source data are provided as a file.

Article Snippet: Initially, purified SOX13 protein (0.17 mg/mL) was dissolved in PBS and then immobilized onto the CM5 chip (GE Healthcare, USA).

Techniques: ChIP-sequencing, Over Expression, Luciferase, Construct, Two Tailed Test

A Effect of SCAF1 knockdown and SCAF1 re-expression on Erastin (2 μM) or RSL3 (0.5 μM) sensitivity in the absence or presence of Z-VAD-FMK (10 μM), NSA (1 μM), or Fer-1 (1 μM) in parental versus resistant cells. The cells were treated for 24 h. Cell viability was evaluated with a CellTiter-Glo luminescent cell viability assay ( n = 3 independent experiments). Data are presented as mean values ± SD. B SNU-668 Erastin resis cells were treated with lentiviruses encoding SCAF1 shRNA-1 or shRNA-2 or a scrambled shRNA alone and/or resistant SOX13 cDNA (SOX13 resis ), or in combination with Erastin (2 μM) or RSL3 (0.5 μM) for 24 h. Lipid peroxidation was determined with a lipid peroxidation C11-BODIPY assay in SNU-668 Erastin resis cells ( n = 3 independent experiments), and a representative flow cytometry histogram plot is presented. Data are presented as mean values ± SD. C Effect of SOX13 overexpression (Lv-SOX13) alone, SCAF1 KO (sg-SCAF1) alone, or the two in combination on Erastin or RSL3 sensitivity in parental SNU-668 and SNU-484 cells. The cells were treated for 24 h ( n = 3 independent experiments). Data are presented as mean values ± SD. Statistical significance in ( A – C ) is determined by two-tailed unpaired t test. Source data are provided as a file.

Journal: Nature Communications

Article Title: Targeting SOX13 inhibits assembly of respiratory chain supercomplexes to overcome ferroptosis resistance in gastric cancer

doi: 10.1038/s41467-024-48307-z

Figure Lengend Snippet: A Effect of SCAF1 knockdown and SCAF1 re-expression on Erastin (2 μM) or RSL3 (0.5 μM) sensitivity in the absence or presence of Z-VAD-FMK (10 μM), NSA (1 μM), or Fer-1 (1 μM) in parental versus resistant cells. The cells were treated for 24 h. Cell viability was evaluated with a CellTiter-Glo luminescent cell viability assay ( n = 3 independent experiments). Data are presented as mean values ± SD. B SNU-668 Erastin resis cells were treated with lentiviruses encoding SCAF1 shRNA-1 or shRNA-2 or a scrambled shRNA alone and/or resistant SOX13 cDNA (SOX13 resis ), or in combination with Erastin (2 μM) or RSL3 (0.5 μM) for 24 h. Lipid peroxidation was determined with a lipid peroxidation C11-BODIPY assay in SNU-668 Erastin resis cells ( n = 3 independent experiments), and a representative flow cytometry histogram plot is presented. Data are presented as mean values ± SD. C Effect of SOX13 overexpression (Lv-SOX13) alone, SCAF1 KO (sg-SCAF1) alone, or the two in combination on Erastin or RSL3 sensitivity in parental SNU-668 and SNU-484 cells. The cells were treated for 24 h ( n = 3 independent experiments). Data are presented as mean values ± SD. Statistical significance in ( A – C ) is determined by two-tailed unpaired t test. Source data are provided as a file.

Article Snippet: Initially, purified SOX13 protein (0.17 mg/mL) was dissolved in PBS and then immobilized onto the CM5 chip (GE Healthcare, USA).

Techniques: Expressing, Cell Viability Assay, shRNA, Flow Cytometry, Over Expression, Two Tailed Test

A Kaplan–Meier survival curves and log-rank test analysis of DFS (left) and OS (right) rates in GC cancer patients who underwent cisplatin-based adjuvant chemotherapy. The GC patients were grouped according to the median coexpression value within their tumors. B Immunochemistry scoring of 4-hydroxy-2-nonenal (4-HNE) staining of matched GC samples before and after cisplatin-based chemotherapy from 52 GC patients. Error bars are means ± SD, n = 3 randomly selected magnification fields. C Representative images of 4-HNE, SOX13 and SCAF1 immunohistochemical staining of matched GC samples from the same patients before or after chemotherapy. Scale bar: 50 μm. Two-tailed paired t test ( B ) or two-sided log-rank test ( A ). Source data are provided as a file.

Journal: Nature Communications

Article Title: Targeting SOX13 inhibits assembly of respiratory chain supercomplexes to overcome ferroptosis resistance in gastric cancer

doi: 10.1038/s41467-024-48307-z

Figure Lengend Snippet: A Kaplan–Meier survival curves and log-rank test analysis of DFS (left) and OS (right) rates in GC cancer patients who underwent cisplatin-based adjuvant chemotherapy. The GC patients were grouped according to the median coexpression value within their tumors. B Immunochemistry scoring of 4-hydroxy-2-nonenal (4-HNE) staining of matched GC samples before and after cisplatin-based chemotherapy from 52 GC patients. Error bars are means ± SD, n = 3 randomly selected magnification fields. C Representative images of 4-HNE, SOX13 and SCAF1 immunohistochemical staining of matched GC samples from the same patients before or after chemotherapy. Scale bar: 50 μm. Two-tailed paired t test ( B ) or two-sided log-rank test ( A ). Source data are provided as a file.

Article Snippet: Initially, purified SOX13 protein (0.17 mg/mL) was dissolved in PBS and then immobilized onto the CM5 chip (GE Healthcare, USA).

Techniques: Adjuvant, Staining, Immunohistochemical staining, Two Tailed Test

Parental and resistant cells. Oxygen consumption analysis was performed in ( A ) intact cells or ( B ) isolated mitochondria using pyruvate-malate or succinate as specific substrates, respectively ( n = 3 independent experiments). Data are presented as mean values ± SD. C Mitochondrial enzymatic activities of complex I (CI), complex IV (CIV), complex II (CII), CI+complex III (CIII), and CII + III normalized to citrate synthase (CS) levels in parental and resistant SNU-668 cells ( n = 3 independent experiments). Data are presented as mean values ± SD. D Western blot analysis of the indicated proteins, including CI, CIII, CIV, and CII, after blue native PAGE (BN-PAGE) of digitonin-solubilized mitochondria from parental and resistant SNU-668 cells (Representative plot of experiments repeated in triplicate). E Oxygen consumption rates (OCR) of isolated mitochondria from parental SNU-668 cells with SOX13 overexpression (Lv-SOX13) alone, SCAF1 KO (sg-SCAF1) alone or the two in combination ( n = 3 independent experiments). Data are presented as mean values ± SD. F SC levels in parental SNU-668 cells with SOX13 overexpression (Lv-SOX13) alone, SCAF1 KO (sg-SCAF1) alone or the two in combination (Representative plot of experiments repeated in triplicate). G Schematic illustrating the metabolic consequences from loss of supercomplex formation in GC cells. Intact supercomplexes (left) and loss of supercomplexes (right) are demonstrated using the respirasome (complexes I, III 2 , and IV). H NADPH levels and NADPH/NADP + in parental and resistant cells ( n = 3 independent experiments). Data are presented as mean values ± SD. I Abundance of NADPH in the blood of cisplatin treatment nonresponsive patients (TRG ≥ 3, n = 13) compared to patients sensitive to cisplatin (TRG ≤ 2, n = 12) from cohort 2. Data are presented as mean values ± SD. Two-tailed unpaired t -test ( A – C , E , H ) or two-tailed Mann–Whitney test ( I ). Source data are provided as a file.

Journal: Nature Communications

Article Title: Targeting SOX13 inhibits assembly of respiratory chain supercomplexes to overcome ferroptosis resistance in gastric cancer

doi: 10.1038/s41467-024-48307-z

Figure Lengend Snippet: Parental and resistant cells. Oxygen consumption analysis was performed in ( A ) intact cells or ( B ) isolated mitochondria using pyruvate-malate or succinate as specific substrates, respectively ( n = 3 independent experiments). Data are presented as mean values ± SD. C Mitochondrial enzymatic activities of complex I (CI), complex IV (CIV), complex II (CII), CI+complex III (CIII), and CII + III normalized to citrate synthase (CS) levels in parental and resistant SNU-668 cells ( n = 3 independent experiments). Data are presented as mean values ± SD. D Western blot analysis of the indicated proteins, including CI, CIII, CIV, and CII, after blue native PAGE (BN-PAGE) of digitonin-solubilized mitochondria from parental and resistant SNU-668 cells (Representative plot of experiments repeated in triplicate). E Oxygen consumption rates (OCR) of isolated mitochondria from parental SNU-668 cells with SOX13 overexpression (Lv-SOX13) alone, SCAF1 KO (sg-SCAF1) alone or the two in combination ( n = 3 independent experiments). Data are presented as mean values ± SD. F SC levels in parental SNU-668 cells with SOX13 overexpression (Lv-SOX13) alone, SCAF1 KO (sg-SCAF1) alone or the two in combination (Representative plot of experiments repeated in triplicate). G Schematic illustrating the metabolic consequences from loss of supercomplex formation in GC cells. Intact supercomplexes (left) and loss of supercomplexes (right) are demonstrated using the respirasome (complexes I, III 2 , and IV). H NADPH levels and NADPH/NADP + in parental and resistant cells ( n = 3 independent experiments). Data are presented as mean values ± SD. I Abundance of NADPH in the blood of cisplatin treatment nonresponsive patients (TRG ≥ 3, n = 13) compared to patients sensitive to cisplatin (TRG ≤ 2, n = 12) from cohort 2. Data are presented as mean values ± SD. Two-tailed unpaired t -test ( A – C , E , H ) or two-tailed Mann–Whitney test ( I ). Source data are provided as a file.

Article Snippet: Initially, purified SOX13 protein (0.17 mg/mL) was dissolved in PBS and then immobilized onto the CM5 chip (GE Healthcare, USA).

Techniques: Isolation, Western Blot, Blue Native PAGE, Over Expression, Two Tailed Test, MANN-WHITNEY

A SOX13-KO (sg-SOX13), SCAF1-overexpressing (Lv-SCAF1) or control YTH16 (m) cells were grown as xenografts. Antibody to mouse PD1 treatment or lipoxstatin alone or in combination started on Day 7 after injection of C57BL/6 mice with cells to form xenografts. Tumor growth ( B ) and weight change ( C ) are shown ( n = 4 mice per group). Data are presented as mean values ± SD. D Flow cytometry analysis of BODIPY fluorescence in CD45 + and CD45 − tumor cells isolated from control YTH16 (m) derived xenografts treated with antibody to mouse PD1. E Representative flow cytometry analysis and quantification of BODIPY fluorescence in CD45 − tumor cells from YTH16 (m)-derived xenografts with the treatment described above (data from randomly selected three tumors from each group). Data are presented as mean values ± SD. Statistical significance in (B,C,E) is determined by two-tailed unpaired t -test. Source data are provided as a file.

Journal: Nature Communications

Article Title: Targeting SOX13 inhibits assembly of respiratory chain supercomplexes to overcome ferroptosis resistance in gastric cancer

doi: 10.1038/s41467-024-48307-z

Figure Lengend Snippet: A SOX13-KO (sg-SOX13), SCAF1-overexpressing (Lv-SCAF1) or control YTH16 (m) cells were grown as xenografts. Antibody to mouse PD1 treatment or lipoxstatin alone or in combination started on Day 7 after injection of C57BL/6 mice with cells to form xenografts. Tumor growth ( B ) and weight change ( C ) are shown ( n = 4 mice per group). Data are presented as mean values ± SD. D Flow cytometry analysis of BODIPY fluorescence in CD45 + and CD45 − tumor cells isolated from control YTH16 (m) derived xenografts treated with antibody to mouse PD1. E Representative flow cytometry analysis and quantification of BODIPY fluorescence in CD45 − tumor cells from YTH16 (m)-derived xenografts with the treatment described above (data from randomly selected three tumors from each group). Data are presented as mean values ± SD. Statistical significance in (B,C,E) is determined by two-tailed unpaired t -test. Source data are provided as a file.

Article Snippet: Initially, purified SOX13 protein (0.17 mg/mL) was dissolved in PBS and then immobilized onto the CM5 chip (GE Healthcare, USA).

Techniques: Injection, Flow Cytometry, Fluorescence, Isolation, Derivative Assay, Two Tailed Test

A A schematic diagram demonstrating the screening strategy for SOX13-targeting compounds. B Effect of the top 40 candidate compounds (10 μM) on RSL3 (0.5 μM) sensitivity in Erastin resis SNU-668 cells. The cells were pretreated with candidate compounds (10 μM) for 12 h prior to being exposed to RSL3 (0.5 μM) for 24 h. Cell viability was examined ( n = 3 independent experiments). Data are presented as mean values ± SD. C SPR analysis of the interaction between zanamivir and SOX13 protein. D The Erastin resis SNU-668 cells cells were pretreated with zanamivir (0 μM, 5 μM, 10 μM) for 12 h and then exposed to Erastin (2 μM) or RSL3 (0.5 μM) for 24 h. Lipid peroxidation was determined using a lipid peroxidation C11-BODIPY assay ( n = 3 independent experiments), and representative flow cytometry histogram plot is presented. Data are presented as mean values ± SD. Tumors from Erastin resis SNU-668 cells transfected with desired vector were treated with zanamivir, cisplatin and IKE. Representative images of tumors formed ( E ), tumor growth curves ( F ) and tumor weights ( G ) are shown. Treatment with cisplatin (4 mg/kg, i.p., once weekly), IKE (20 mg/kg, i.p., once daily), zanamivir (5 mg/kg, i.p., once daily) or PBS (100 µl, i.p., once daily) started on Day 7 and lasted for 3 consecutive weeks ( n = 4 mice per group). Data are presented as mean values ± SD. Statistical significance in ( D , F , G ) is determined by two-tailed unpaired t test. Source data are provided as a file.

Journal: Nature Communications

Article Title: Targeting SOX13 inhibits assembly of respiratory chain supercomplexes to overcome ferroptosis resistance in gastric cancer

doi: 10.1038/s41467-024-48307-z

Figure Lengend Snippet: A A schematic diagram demonstrating the screening strategy for SOX13-targeting compounds. B Effect of the top 40 candidate compounds (10 μM) on RSL3 (0.5 μM) sensitivity in Erastin resis SNU-668 cells. The cells were pretreated with candidate compounds (10 μM) for 12 h prior to being exposed to RSL3 (0.5 μM) for 24 h. Cell viability was examined ( n = 3 independent experiments). Data are presented as mean values ± SD. C SPR analysis of the interaction between zanamivir and SOX13 protein. D The Erastin resis SNU-668 cells cells were pretreated with zanamivir (0 μM, 5 μM, 10 μM) for 12 h and then exposed to Erastin (2 μM) or RSL3 (0.5 μM) for 24 h. Lipid peroxidation was determined using a lipid peroxidation C11-BODIPY assay ( n = 3 independent experiments), and representative flow cytometry histogram plot is presented. Data are presented as mean values ± SD. Tumors from Erastin resis SNU-668 cells transfected with desired vector were treated with zanamivir, cisplatin and IKE. Representative images of tumors formed ( E ), tumor growth curves ( F ) and tumor weights ( G ) are shown. Treatment with cisplatin (4 mg/kg, i.p., once weekly), IKE (20 mg/kg, i.p., once daily), zanamivir (5 mg/kg, i.p., once daily) or PBS (100 µl, i.p., once daily) started on Day 7 and lasted for 3 consecutive weeks ( n = 4 mice per group). Data are presented as mean values ± SD. Statistical significance in ( D , F , G ) is determined by two-tailed unpaired t test. Source data are provided as a file.

Article Snippet: Initially, purified SOX13 protein (0.17 mg/mL) was dissolved in PBS and then immobilized onto the CM5 chip (GE Healthcare, USA).

Techniques: Flow Cytometry, Transfection, Plasmid Preparation, Two Tailed Test

A The Erastin resis SNU-668 cells or RSL3 resis SNU-484 cells were pretreated with zanamivir (0 μM, 5 μM, 10 μM, 20 μM) for 36 h. Western blot analysis of SOX13, and SCAF1 protein expression levels (Representative plot of experiments repeated in triplicate). B The Erastin resis SNU-668 cells or RSL3 resis SNU-484 cells were treated with cycloheximide (100 μg/mL) as indicated in the presence or absence of zanamivir (10 μM) ( n = 3 independent experiments). Data are presented as mean values ± SD. C The SNU-668 cells or SNU-484 cells were transfected with the desired plasmids, 36 h after transfection, they were treated with zanamivir (10 μM) for 24 h, and then subjected to IP using anti-Flag antibody followed by Western blot analysis (Representative plot of experiments repeated in triplicate). D Depletion of TRIM25 increased while forced expression of TRIM25 decreased the protein level of SOX13 in Erastin resis SNU-668 cells or RSL3 resis SNU-484 cells (Representative plot of experiments repeated in triplicate). E Western blot analysis of SOX13 and TRIM25 protein levels in Erastin resis SNU-668 cells overexpressing TRIM25-WT, TRIM25-2EA or vector control (Representative plot of experiments repeated in triplicate). F Co-IP assay was used to determine the binding of SOX13 with TRIM25 in the presence or absence of zanamivir (Representative plot of experiments repeated in triplicate). G Ferroptosis-sensitization mechanism of SOX13-targeting compound zanamivir. Zanamivir targets SOX13 protein and thus downregulates SCAF1, which leads to decreased supercomplexes (SCs) assembly, mitochondrial respiration, mitochondrial energetics, and increased ferroptosis sensitivity. Statistical significance in ( B ) is determined by two-tailed unpaired t test. Source data are provided as a file.

Journal: Nature Communications

Article Title: Targeting SOX13 inhibits assembly of respiratory chain supercomplexes to overcome ferroptosis resistance in gastric cancer

doi: 10.1038/s41467-024-48307-z

Figure Lengend Snippet: A The Erastin resis SNU-668 cells or RSL3 resis SNU-484 cells were pretreated with zanamivir (0 μM, 5 μM, 10 μM, 20 μM) for 36 h. Western blot analysis of SOX13, and SCAF1 protein expression levels (Representative plot of experiments repeated in triplicate). B The Erastin resis SNU-668 cells or RSL3 resis SNU-484 cells were treated with cycloheximide (100 μg/mL) as indicated in the presence or absence of zanamivir (10 μM) ( n = 3 independent experiments). Data are presented as mean values ± SD. C The SNU-668 cells or SNU-484 cells were transfected with the desired plasmids, 36 h after transfection, they were treated with zanamivir (10 μM) for 24 h, and then subjected to IP using anti-Flag antibody followed by Western blot analysis (Representative plot of experiments repeated in triplicate). D Depletion of TRIM25 increased while forced expression of TRIM25 decreased the protein level of SOX13 in Erastin resis SNU-668 cells or RSL3 resis SNU-484 cells (Representative plot of experiments repeated in triplicate). E Western blot analysis of SOX13 and TRIM25 protein levels in Erastin resis SNU-668 cells overexpressing TRIM25-WT, TRIM25-2EA or vector control (Representative plot of experiments repeated in triplicate). F Co-IP assay was used to determine the binding of SOX13 with TRIM25 in the presence or absence of zanamivir (Representative plot of experiments repeated in triplicate). G Ferroptosis-sensitization mechanism of SOX13-targeting compound zanamivir. Zanamivir targets SOX13 protein and thus downregulates SCAF1, which leads to decreased supercomplexes (SCs) assembly, mitochondrial respiration, mitochondrial energetics, and increased ferroptosis sensitivity. Statistical significance in ( B ) is determined by two-tailed unpaired t test. Source data are provided as a file.

Article Snippet: Initially, purified SOX13 protein (0.17 mg/mL) was dissolved in PBS and then immobilized onto the CM5 chip (GE Healthcare, USA).

Techniques: Western Blot, Expressing, Transfection, Plasmid Preparation, Co-Immunoprecipitation Assay, Binding Assay, Two Tailed Test

Journal: Brain

Article Title: POU3F2 regulates canonical Wnt signalling via SOX13 and ADNP to expand the neural progenitor population

doi: 10.1093/brain/awaf221

Figure Lengend Snippet: POU3F2 activates expression of SOX13 and ADNP to regulate canonical Wnt signalling in NPCs. ( A ) Potential direct effectors of POU3F2 were prioritized if they belonged to the TCF-dependent signalling in response to Wnt Reactome pathway (R-HSA-201681). ( B ) Quantification of ADNP protein expression measured via western blotting in POU3F2 WT and POU3F2 MUT NPCs, mean ± SEM ( n = 4 differentiations). Paired t -test, * P < 0.05. ( C ) Quantification of SOX13 protein expression measured via western blotting in POU3F2 WT and POU3F2 MUT NPCs, mean ± SEM ( n = 5 differentiations). Paired t -test, ** P < 0.01. ( D ) Schematic of experimental design for overexpression of a gene of interest followed by the SUPERTOPFLASH canonical Wnt reporter assay. ( E ) Baseline canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or ADNP, mean ± SEM ( n = 3 differentiations). Mixed-effects model with post hoc Sidak's test, ns = not significant. ( F ) Activated canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or ADNP, mean ± SEM ( n = 3 differentiations). Mixed-effects model with post hoc Sidak's test, * P < 0.05, ** P < 0.01. ( G ) Baseline canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or SOX13, mean ± SEM ( n = 3 differentiations). GFP control data represented here is included in . Mixed-effects model with post hoc Sidak's test, * P < 0.05, ** P < 0.01. ( H ) Activated canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or SOX13, mean ± SEM ( n = 3 differentiations). GFP control data represented here is included in . Mixed-effects model with post hoc Sidak's test, * P < 0.05, *** P < 0.001. ( I ) Genomic structure of SOX13 . Box above gene indicates relative position of gRNA target sequence. Western blot validation of SOX13 knockdown (GAPDH = loading control). ( J ) Gene ontology pathway enrichment results of differential gene expression in SOX13 MUT NPCs, separated by directionality of log 2 fold change. ( K ) GSEA of Wnt-activated targets and G2/M progenitor markers in SOX13 MUT versus SOX13 WT differential expression. ( L ) Model of POU3F2-mediated regulation of canonical Wnt signalling and progenitor expansion via transcription of ADNP and SOX13 . Schematics created in BioRender. Benoit, C. (2025) https://BioRender.com/35q22ly , https://BioRender.com/baxexcu . gRNA = guide RNA; GSEA = gene set enrichment analysis; MUT = mutant; NPC = neural progenitor cell; SEM = standard error of the mean; WT = wild-type.

Article Snippet: Primary antibodies diluted in blocking buffer were applied overnight at 4°C: POU3F2 (CST, Cat. No. 12137S, 1:1000), GAPDH (Proteintech, Cat. No. 60004-1-Ig, 1:10 000), β-catenin (CST, Cat. No. 8480S, 1:1000), Nestin (Novus Biologicals, Cat. No. MAB1259, 1:1000), TUJ1 (Novus Biologicals, Cat. No. NB100-1612, 1:1000), POU3F2 (Santa Cruz, Cat. No. sc-393324, 1:1000), HA-Tag (CST, Cat. No. C29F4, 1:500), ADNP (Abcam, Cat. No. ab300114, 1:500), SOX13 (Proteintech, Cat. No. 18902-1, 1:1000).

Techniques: Expressing, Western Blot, Over Expression, Reporter Assay, Negative Control, Control, Sequencing, Biomarker Discovery, Knockdown, Gene Expression, Quantitative Proteomics, Mutagenesis

Descriptive statistics of the analysed immunity parameters and piglet survival traits

Journal: Veterinary Research

Article Title: Insights into genetic determinants of piglet survival during a PRRSV outbreak

doi: 10.1186/s13567-024-01421-8

Figure Lengend Snippet: Descriptive statistics of the analysed immunity parameters and piglet survival traits

Article Snippet: SOX13 primer pair (F-5′-AAGCCAAAGACGTCAAAGGGA-3′ and R-5′-TCCCGAAGGGTGGACAGTT-3′) was designed using PrimerExpress 2.0 software (Applied Biosystems).

Techniques:

Kaplan–Meier survival functions stratified by each immunity trait. A IgG levels were categorized as: (IgG3 = IgG < 3.0, IgG4 = 3.0 < IgG < 4.5, IgG5 = 4.5 < IgG < 6.0, IgG6 = IgG > 6.0). B SOX13 mRNA expression levels were categorized as: (SOX2 = SOX13 < 3.0, SOX4 = 3.0 < SOX13 < 5.0, SOX6 = 5.0 < SOX13 < 7.0, SOX8 = 7.0 < SOX13 < 9.0, SOX10 = SOX13 > 9.0).

Journal: Veterinary Research

Article Title: Insights into genetic determinants of piglet survival during a PRRSV outbreak

doi: 10.1186/s13567-024-01421-8

Figure Lengend Snippet: Kaplan–Meier survival functions stratified by each immunity trait. A IgG levels were categorized as: (IgG3 = IgG < 3.0, IgG4 = 3.0 < IgG < 4.5, IgG5 = 4.5 < IgG < 6.0, IgG6 = IgG > 6.0). B SOX13 mRNA expression levels were categorized as: (SOX2 = SOX13 < 3.0, SOX4 = 3.0 < SOX13 < 5.0, SOX6 = 5.0 < SOX13 < 7.0, SOX8 = 7.0 < SOX13 < 9.0, SOX10 = SOX13 > 9.0).

Article Snippet: SOX13 primer pair (F-5′-AAGCCAAAGACGTCAAAGGGA-3′ and R-5′-TCCCGAAGGGTGGACAGTT-3′) was designed using PrimerExpress 2.0 software (Applied Biosystems).

Techniques: Expressing

Review

sox13 (Proteintech)

Structured Review

Images

1) Product Images from "POU3F2 regulates canonical Wnt signalling via SOX13 and ADNP to expand the neural progenitor population"

Related Articles

Incubation:

Blocking Assay:

Immunohistochemistry:

Western Blot:

Similar Products

Image Search Results