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lps-sm ultrapure  (InvivoGen)


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    InvivoGen lps-sm ultrapure
    Lps Sm Ultrapure, supplied by InvivoGen, used in various techniques. Bioz Stars score: 96/100, based on 22444 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lps-sm ultrapure/product/InvivoGen
    Average 96 stars, based on 22444 article reviews
    lps-sm ultrapure - by Bioz Stars, 2026-06
    96/100 stars

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    ( A-B ). Cell death of THP-1 macrophages upon infection with S. flexneri 2457T ( A ), or electroporation with S. <t>enterica</t> serovar Minnesota LPS ( B ), measured by LDH release, as percentage of Triton X-100-induced cell lysis. N ≥ 4 biological replicates. ( C-D ). NLRP11 is required for the processing of CASP4 and gasdermin D (GSDMD) induced by S. flexneri infection ( C ), or S. enterica LPS electroporation ( D ). CASP4 is required for GSDMD processing induced by S. flexneri infection ( C ), or S. enterica LPS electroporation ( D ). Processing of CASP4 yields a p32 and/or p20 polypeptide, and processing of GSDMD yields a p30 polypeptide. Representative western blots. Molecular weight markers in kDa. DKO, NLRP11 −/− CASP4 −/− double knockout. Mean ± SEM. Two-way ANOVA with Tukey’s post hoc test *, P<0.05; **, P<0.01; ****, P<0.0001.
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    ( A-B ). Cell death of THP-1 macrophages upon infection with S. flexneri 2457T ( A ), or electroporation with S. enterica serovar Minnesota LPS ( B ), measured by LDH release, as percentage of Triton X-100-induced cell lysis. N ≥ 4 biological replicates. ( C-D ). NLRP11 is required for the processing of CASP4 and gasdermin D (GSDMD) induced by S. flexneri infection ( C ), or S. enterica LPS electroporation ( D ). CASP4 is required for GSDMD processing induced by S. flexneri infection ( C ), or S. enterica LPS electroporation ( D ). Processing of CASP4 yields a p32 and/or p20 polypeptide, and processing of GSDMD yields a p30 polypeptide. Representative western blots. Molecular weight markers in kDa. DKO, NLRP11 −/− CASP4 −/− double knockout. Mean ± SEM. Two-way ANOVA with Tukey’s post hoc test *, P<0.05; **, P<0.01; ****, P<0.0001.

    Journal: bioRxiv

    Article Title: NLRP11 promotes non-canonical inflammasome activation in human macrophages by enhancing caspase-4 recognition of cytosolic lipopolysaccharide

    doi: 10.64898/2026.02.02.703338

    Figure Lengend Snippet: ( A-B ). Cell death of THP-1 macrophages upon infection with S. flexneri 2457T ( A ), or electroporation with S. enterica serovar Minnesota LPS ( B ), measured by LDH release, as percentage of Triton X-100-induced cell lysis. N ≥ 4 biological replicates. ( C-D ). NLRP11 is required for the processing of CASP4 and gasdermin D (GSDMD) induced by S. flexneri infection ( C ), or S. enterica LPS electroporation ( D ). CASP4 is required for GSDMD processing induced by S. flexneri infection ( C ), or S. enterica LPS electroporation ( D ). Processing of CASP4 yields a p32 and/or p20 polypeptide, and processing of GSDMD yields a p30 polypeptide. Representative western blots. Molecular weight markers in kDa. DKO, NLRP11 −/− CASP4 −/− double knockout. Mean ± SEM. Two-way ANOVA with Tukey’s post hoc test *, P<0.05; **, P<0.01; ****, P<0.0001.

    Article Snippet: Cells were treated with either 2 μg/mL S. enterica serovar Minnesota R595 LPS (InvivoGen, tlrl-smlps) or 10 μg/mL E. coli O111:B4 LPS (InvivoGen, tlrl-eblps).

    Techniques: Infection, Electroporation, Lysis, Western Blot, Molecular Weight, Double Knockout