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antibodies slc3a2  (Proteintech)


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    Structured Review

    Proteintech antibodies slc3a2
    Antibodies Slc3a2, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 132 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/slc3a2/CD98%2FSLC3A2+Antibody/10__1007_slash_s13402___026___01299___y-159-10-13
    Average 96 stars, based on 132 article reviews
    antibodies slc3a2 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    other:

    Article Title: Diterpenoids and triterpenoids from the roots of Tripterygium regelii and their inhibitory effects on colorectal cancer cells.
    Article Snippet: An anti-fluorescence quenching agent was then added for coverslip mounting, and images were acquired using an inverted fluorescence microscope (Olympus, Model 1X73) to obtain the assay results.

    Control:

    Article Title: Diterpenoids and triterpenoids from the roots of Tripterygium regelii and their inhibitory effects on colorectal cancer cells
    Article Snippet: An anti-fluorescence quenching agent was then added for coverslip mounting, and images were acquired using an inverted fluorescence microscope (Olympus, Model 1X73) to obtain the assay results. .. Membranes were probed with primary antibodies against SLC7A11 ( AWA00502 , Abiowell), SLC3A2 ( AWA61227 , Abiowell), GPX4 ( AWA11352 , Abiowell), and GAPDH (81640-5-RR, Proteintech), where GAPDH served as a loading control to ensure equal protein loading. .. Proteins were visualized by autoradiography and quantified using ImageJ software.

    Membrane:

    Article Title: Integrated multi-omics analysis reveals GPD1L-mediated dysregulation of glycerophospholipid metabolism facilitates the progression of lung adenocarcinoma.
    Article Snippet: .. And the membrane was incubated with the primary antibodies, including GPD1L (17263-1-AP, proteintech, USA), HIF1A (20960-1-AP, proteintech, USA), Caspase-3/P17/P19 (19677-1-AP, proteintech, USA), GPX4 (67763-1-Ig, proteintech, USA), SLC7A11 (26864-1-AP, proteintech, USA), SLC3A2 (66883-1-Ig, proteintech, USA), pan-AKT (4691T, Cell Signaling Technology, USA), p-AKT (4060S, Cell Signaling Technology, USA), NRF2 (12721, Cell Signaling Technology, USA), HIF1α (ab179483, abcam, USA), Hydroxy-HIF-1 alpha (3434T, Cell Signaling Technology, USA), β-Actin (ab6276, abcam, USA), overnight at 4 °C. .. And the following day, we incubated the second antibody (abcam, UK) for 1-2 hours at ambient temperature.

    Article Title: Integrated multi-omics analysis reveals GPD1L-mediated dysregulation of glycerophospholipid metabolism facilitates the progression of lung adenocarcinoma
    Article Snippet: .. And the membrane was incubated with the primary antibodies, including GPD1L (17,263–1-AP, proteintech, USA), HIF1A (20,960–1-AP, proteintech, USA), Caspase-3/P17/P19 (19,677–1-AP, proteintech, USA), GPX4 (67,763–1-Ig, proteintech, USA), SLC7A11 (26,864–1-AP, proteintech, USA), SLC3A2 (66,883–1-Ig, proteintech, USA), pan-AKT (4691 T, Cell Signaling Technology, USA), p-AKT (4060S, Cell Signaling Technology, USA), NRF2 (12,721, Cell Signaling Technology, USA), HIF1α (ab179483, abcam, USA), Hydroxy-HIF-1 alpha (3434 T, Cell Signaling Technology, USA), β-Actin (ab6276, abcam, USA), overnight at 4 °C. .. And the following day, we incubated the second antibody (abcam, UK) for 1–2 h at ambient temperature.

    Incubation:

    Article Title: Integrated multi-omics analysis reveals GPD1L-mediated dysregulation of glycerophospholipid metabolism facilitates the progression of lung adenocarcinoma.
    Article Snippet: .. And the membrane was incubated with the primary antibodies, including GPD1L (17263-1-AP, proteintech, USA), HIF1A (20960-1-AP, proteintech, USA), Caspase-3/P17/P19 (19677-1-AP, proteintech, USA), GPX4 (67763-1-Ig, proteintech, USA), SLC7A11 (26864-1-AP, proteintech, USA), SLC3A2 (66883-1-Ig, proteintech, USA), pan-AKT (4691T, Cell Signaling Technology, USA), p-AKT (4060S, Cell Signaling Technology, USA), NRF2 (12721, Cell Signaling Technology, USA), HIF1α (ab179483, abcam, USA), Hydroxy-HIF-1 alpha (3434T, Cell Signaling Technology, USA), β-Actin (ab6276, abcam, USA), overnight at 4 °C. .. And the following day, we incubated the second antibody (abcam, UK) for 1-2 hours at ambient temperature.

    Article Title: GBP2 condensates promote ferroptosis to sensitize anti-PD-L1 immunotherapy in melanoma.
    Article Snippet: Then, 30 μg of protein from each group was run on an 10% SDS-PAGE gel and transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, USA). .. The PVDF membranes were blocked with 5% fat-free milk and incubated overnight at 4 °C with the appropriate primary antibodies: GBP2 (11854-1-AP, Proteintech, USA), p-STAT1 (#9167, CST, USA), STAT1 (#14994, CST, USA), SLC7A11 (#98051, CST, USA), SLC3A2 (15193-1-AP, Proteintech, USA), ACSL4 (22401-1-AP, Proteintech, USA), PD-L1 (17952-1-AP, Proteintech, USA), HMGB1 (10829-1-AP, Proteintech, USA), SHP1 (24546-1-AP, Proteintech, USA), and GAPDH (10494-1-AP, Proteintech, USA). .. Finally, enhanced chemiluminescence reagents (Thermo, USA) were applied to detect the bands after incubation with secondary antibody (GB23303, Servicebio, China).

    Article Title: GBP2 condensates promote ferroptosis to sensitize anti-PD-L1 immunotherapy in melanoma
    Article Snippet: Then, 30 μg of protein from each group was run on an 10% SDS-PAGE gel and transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, USA). .. The PVDF membranes were blocked with 5% fat-free milk and incubated overnight at 4 °C with the appropriate primary antibodies: GBP2 (11854-1-AP, Proteintech, USA), p-STAT1 (#9167, CST, USA), STAT1 (#14994, CST, USA), SLC7A11 (#98051, CST, USA), SLC3A2 (15193-1-AP, Proteintech, USA), ACSL4 (22401-1-AP, Proteintech, USA), PD-L1 (17952-1-AP, Proteintech, USA), HMGB1 (10829-1-AP, Proteintech, USA), SHP1 (24546-1-AP, Proteintech, USA), and GAPDH (10494-1-AP, Proteintech, USA). .. Finally, enhanced chemiluminescence reagents (Thermo, USA) were applied to detect the bands after incubation with secondary antibody (GB23303, Servicebio, China).

    Article Title: Integrated multi-omics analysis reveals GPD1L-mediated dysregulation of glycerophospholipid metabolism facilitates the progression of lung adenocarcinoma
    Article Snippet: .. And the membrane was incubated with the primary antibodies, including GPD1L (17,263–1-AP, proteintech, USA), HIF1A (20,960–1-AP, proteintech, USA), Caspase-3/P17/P19 (19,677–1-AP, proteintech, USA), GPX4 (67,763–1-Ig, proteintech, USA), SLC7A11 (26,864–1-AP, proteintech, USA), SLC3A2 (66,883–1-Ig, proteintech, USA), pan-AKT (4691 T, Cell Signaling Technology, USA), p-AKT (4060S, Cell Signaling Technology, USA), NRF2 (12,721, Cell Signaling Technology, USA), HIF1α (ab179483, abcam, USA), Hydroxy-HIF-1 alpha (3434 T, Cell Signaling Technology, USA), β-Actin (ab6276, abcam, USA), overnight at 4 °C. .. And the following day, we incubated the second antibody (abcam, UK) for 1–2 h at ambient temperature.

    FLAG-tag:

    Article Title: Bovine viral diarrhea virus E2 targets SLC3A2 to modulate lipid peroxidation for replication advantage.
    Article Snippet: Pestiviruses, part of the Flaviviridae family, are responsible for major livestock diseases such as bovine viral diarrhea, classical swine fever, and border disease.. These infections lead to substantial economic losses worldwide.. However, their pathogenesis remains incompletely understood, limiting the development of effective control strategies.



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    ABclonal Biotechnology antibodies targeting slc3a2
    Derepression of ferroptosis decreases cell proliferation in PSC (A) Western blot showing the knockdown efficiency of <t>SLC3A2</t> and FTL in PLA-801D cells. (B) The cell proliferation of PLA-801D cells measured using CCK-8 assays following the knockdown of SLC3A2 (left) and FTL (right), respectively. (C) The cell proliferation of PLA-801D cells measured using colony formation assays following the knockdown of SLC3A2 (upper) and FTL (bottom), respectively. Following the knockdown of SLC3A2 or FTL , PLA-801D cells were plated and cultured for 14 days. All colonies were subsequently stained with crystal violet and quantified using ImageJ software. (D) The extracellular GSH concentration of PLA-801D cells was tested following the knockdown of SLC3A2 . (E) Following the knockdown of FTL , PLA-801D cells were stained by FerroOrange and quantified using the microplate reader, respectively. (F) The lysates of PLA-801D cells were immunoblotted with the indicated antibodies following the knockdown of FTL . (G) Following the knockdown of SLC3A2 , PLA-801D cells were treated with 10 μM ferrostatin-1 for 24 h. Afterward, the extracellular GSH concentration was tested. (H) Following the knockdown of FTL , PLA-801D cells were treated with 10 μM ferrostatin-1 for 24 h. Then the lysates of PLA-801D cells were immunoblotted with the indicated antibodies. (I) Following the knockdown of SLC3A2 (left) or FTL (right), PLA-801D cells were treated with DMSO or 10 μM ferrostatin-1 for 24 h. Then the cell proliferation of PLA-801D cells was detected by CCK-8 assays, respectively. (J and K) Following the knockdown of SLC3A2 (left) or FTL (right), PLA-801D cells were treated with DMSO or 10 μM ferrostatin-1 for 24 h. Then the fluorescent probes were added into the culture medium to test the relative level of ROS, respectively (J); the cell lysates were collected for bicinchoninic acid assay and the detection of lipid peroxidation MDA (K). (L) The graphic model of the repressed ferroptosis in PSC. NC, negative control; ns, not significant; ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001.
    Antibodies Targeting Slc3a2, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Proteintech 80031 1 rr cd98 slc3a2 rabbit igg n a
    Derepression of ferroptosis decreases cell proliferation in PSC (A) Western blot showing the knockdown efficiency of <t>SLC3A2</t> and FTL in PLA-801D cells. (B) The cell proliferation of PLA-801D cells measured using CCK-8 assays following the knockdown of SLC3A2 (left) and FTL (right), respectively. (C) The cell proliferation of PLA-801D cells measured using colony formation assays following the knockdown of SLC3A2 (upper) and FTL (bottom), respectively. Following the knockdown of SLC3A2 or FTL , PLA-801D cells were plated and cultured for 14 days. All colonies were subsequently stained with crystal violet and quantified using ImageJ software. (D) The extracellular GSH concentration of PLA-801D cells was tested following the knockdown of SLC3A2 . (E) Following the knockdown of FTL , PLA-801D cells were stained by FerroOrange and quantified using the microplate reader, respectively. (F) The lysates of PLA-801D cells were immunoblotted with the indicated antibodies following the knockdown of FTL . (G) Following the knockdown of SLC3A2 , PLA-801D cells were treated with 10 μM ferrostatin-1 for 24 h. Afterward, the extracellular GSH concentration was tested. (H) Following the knockdown of FTL , PLA-801D cells were treated with 10 μM ferrostatin-1 for 24 h. Then the lysates of PLA-801D cells were immunoblotted with the indicated antibodies. (I) Following the knockdown of SLC3A2 (left) or FTL (right), PLA-801D cells were treated with DMSO or 10 μM ferrostatin-1 for 24 h. Then the cell proliferation of PLA-801D cells was detected by CCK-8 assays, respectively. (J and K) Following the knockdown of SLC3A2 (left) or FTL (right), PLA-801D cells were treated with DMSO or 10 μM ferrostatin-1 for 24 h. Then the fluorescent probes were added into the culture medium to test the relative level of ROS, respectively (J); the cell lysates were collected for bicinchoninic acid assay and the detection of lipid peroxidation MDA (K). (L) The graphic model of the repressed ferroptosis in PSC. NC, negative control; ns, not significant; ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001.
    80031 1 Rr Cd98 Slc3a2 Rabbit Igg N A, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Derepression of ferroptosis decreases cell proliferation in PSC (A) Western blot showing the knockdown efficiency of SLC3A2 and FTL in PLA-801D cells. (B) The cell proliferation of PLA-801D cells measured using CCK-8 assays following the knockdown of SLC3A2 (left) and FTL (right), respectively. (C) The cell proliferation of PLA-801D cells measured using colony formation assays following the knockdown of SLC3A2 (upper) and FTL (bottom), respectively. Following the knockdown of SLC3A2 or FTL , PLA-801D cells were plated and cultured for 14 days. All colonies were subsequently stained with crystal violet and quantified using ImageJ software. (D) The extracellular GSH concentration of PLA-801D cells was tested following the knockdown of SLC3A2 . (E) Following the knockdown of FTL , PLA-801D cells were stained by FerroOrange and quantified using the microplate reader, respectively. (F) The lysates of PLA-801D cells were immunoblotted with the indicated antibodies following the knockdown of FTL . (G) Following the knockdown of SLC3A2 , PLA-801D cells were treated with 10 μM ferrostatin-1 for 24 h. Afterward, the extracellular GSH concentration was tested. (H) Following the knockdown of FTL , PLA-801D cells were treated with 10 μM ferrostatin-1 for 24 h. Then the lysates of PLA-801D cells were immunoblotted with the indicated antibodies. (I) Following the knockdown of SLC3A2 (left) or FTL (right), PLA-801D cells were treated with DMSO or 10 μM ferrostatin-1 for 24 h. Then the cell proliferation of PLA-801D cells was detected by CCK-8 assays, respectively. (J and K) Following the knockdown of SLC3A2 (left) or FTL (right), PLA-801D cells were treated with DMSO or 10 μM ferrostatin-1 for 24 h. Then the fluorescent probes were added into the culture medium to test the relative level of ROS, respectively (J); the cell lysates were collected for bicinchoninic acid assay and the detection of lipid peroxidation MDA (K). (L) The graphic model of the repressed ferroptosis in PSC. NC, negative control; ns, not significant; ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001.

    Journal: Cell Reports Medicine

    Article Title: Multi-omics profiling unveils biological and clinical insights into pulmonary sarcomatoid carcinoma

    doi: 10.1016/j.xcrm.2026.102887

    Figure Lengend Snippet: Derepression of ferroptosis decreases cell proliferation in PSC (A) Western blot showing the knockdown efficiency of SLC3A2 and FTL in PLA-801D cells. (B) The cell proliferation of PLA-801D cells measured using CCK-8 assays following the knockdown of SLC3A2 (left) and FTL (right), respectively. (C) The cell proliferation of PLA-801D cells measured using colony formation assays following the knockdown of SLC3A2 (upper) and FTL (bottom), respectively. Following the knockdown of SLC3A2 or FTL , PLA-801D cells were plated and cultured for 14 days. All colonies were subsequently stained with crystal violet and quantified using ImageJ software. (D) The extracellular GSH concentration of PLA-801D cells was tested following the knockdown of SLC3A2 . (E) Following the knockdown of FTL , PLA-801D cells were stained by FerroOrange and quantified using the microplate reader, respectively. (F) The lysates of PLA-801D cells were immunoblotted with the indicated antibodies following the knockdown of FTL . (G) Following the knockdown of SLC3A2 , PLA-801D cells were treated with 10 μM ferrostatin-1 for 24 h. Afterward, the extracellular GSH concentration was tested. (H) Following the knockdown of FTL , PLA-801D cells were treated with 10 μM ferrostatin-1 for 24 h. Then the lysates of PLA-801D cells were immunoblotted with the indicated antibodies. (I) Following the knockdown of SLC3A2 (left) or FTL (right), PLA-801D cells were treated with DMSO or 10 μM ferrostatin-1 for 24 h. Then the cell proliferation of PLA-801D cells was detected by CCK-8 assays, respectively. (J and K) Following the knockdown of SLC3A2 (left) or FTL (right), PLA-801D cells were treated with DMSO or 10 μM ferrostatin-1 for 24 h. Then the fluorescent probes were added into the culture medium to test the relative level of ROS, respectively (J); the cell lysates were collected for bicinchoninic acid assay and the detection of lipid peroxidation MDA (K). (L) The graphic model of the repressed ferroptosis in PSC. NC, negative control; ns, not significant; ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001.

    Article Snippet: Cells were lysed with 1% Triton X-100 buffer (Beyotime, ST1723) and then boiled in 5× loading buffer for 10 min. Each sample was electrophoresed on 10% PAGE gels, then transferred to PVDF membranes and immunoblotted with the antibodies targeting SLC3A2 (Abclonal, A24375), FTL (Abclonal, A11241), GPX4 (Abclonal, A1933) and β-Actin (Abclonal, AC004).

    Techniques: Western Blot, Knockdown, CCK-8 Assay, Cell Culture, Staining, Software, Concentration Assay, Acid Assay, Negative Control