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sirdna  (Spirochrome)


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    Spirochrome sirdna
    Unconstrained mitotic SOX2 activity causes mitotic errors and genomic instability. ( A ) Volcano plot of bulk RNA-seq comparing 3A-SOX2 with WT-SOX2 after 72 h of 1 µg/mL doxycycline (DOX,) induction ( n = 3 replicates). Dashed lines mark P -value = 0.05 and log 2 fold change = 1. ( B ) Gene set enrichment analysis (DNA repair signatures) of the ranked gene list shown in A . ( C ) Time-lapse confocal images of WT-SOX2-GFP and 3A-SOX2-GFP mNSCs stained with <t>SiRDNA</t> (magenta) during mitosis; minutes after nuclear envelope breakdown are indicated. Scale bar, 10 µm. ( D ) Scatter plots (median ± IQR) of the time spent in prometaphase and telophase for WT versus 3A cells (15–20 mitoses; three experiments). ( E , left ) <t>Representative</t> <t>DAPI</t> images of a normal mitosis, lagging chromatin/anaphase bridge, and chromosome compaction defect. ( Right ) Stacked bar chart of event frequencies in parental BL6, WT-SOX2, and 3A-SOX2 lines (≥100 mitoses; three experiments). ( F ) Immunoblots of whole-cell lysates collected 0, 24, and 72 h after DOX induction ( top ) and probed for SOX2, GAPDH, and γH2AX. ( G ) Immunofluorescence for γH2AX (red) in BL6, WT-SOX2, and 3A-SOX2 cells with (DOX + ) or without (DOX − ) induction. Nuclei were stained with DAPI (blue) and SOX2-GFP (green). Scale bar, 10 µm. ( H ) Box and whisker plot quantifying normalized γH2AX intensity per nucleus from images in G (≥150 nuclei; three experiments). ( I ) Immunoblots of BL6 and FLAG-WT-SOX2 or FLAG-3A-SOX2 lines of cells synchronized with 10 µM RO3306 and then released for 0, 45, or 90 min. The dashed line represents where lanes were omitted. Blots were probed for SOX2, phospho-histone H3 (Ser10), γH2AX, and GAPDH.
    Sirdna, supplied by Spirochrome, used in various techniques. Bioz Stars score: 98/100, based on 850 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sirdna/product/Spirochrome
    Average 98 stars, based on 850 article reviews
    sirdna - by Bioz Stars, 2026-02
    98/100 stars

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    1) Product Images from "SOX2 phosphorylation during mitosis limits genomic damage"

    Article Title: SOX2 phosphorylation during mitosis limits genomic damage

    Journal: Genes & Development

    doi: 10.1101/gad.352664.125

    Unconstrained mitotic SOX2 activity causes mitotic errors and genomic instability. ( A ) Volcano plot of bulk RNA-seq comparing 3A-SOX2 with WT-SOX2 after 72 h of 1 µg/mL doxycycline (DOX,) induction ( n = 3 replicates). Dashed lines mark P -value = 0.05 and log 2 fold change = 1. ( B ) Gene set enrichment analysis (DNA repair signatures) of the ranked gene list shown in A . ( C ) Time-lapse confocal images of WT-SOX2-GFP and 3A-SOX2-GFP mNSCs stained with SiRDNA (magenta) during mitosis; minutes after nuclear envelope breakdown are indicated. Scale bar, 10 µm. ( D ) Scatter plots (median ± IQR) of the time spent in prometaphase and telophase for WT versus 3A cells (15–20 mitoses; three experiments). ( E , left ) Representative DAPI images of a normal mitosis, lagging chromatin/anaphase bridge, and chromosome compaction defect. ( Right ) Stacked bar chart of event frequencies in parental BL6, WT-SOX2, and 3A-SOX2 lines (≥100 mitoses; three experiments). ( F ) Immunoblots of whole-cell lysates collected 0, 24, and 72 h after DOX induction ( top ) and probed for SOX2, GAPDH, and γH2AX. ( G ) Immunofluorescence for γH2AX (red) in BL6, WT-SOX2, and 3A-SOX2 cells with (DOX + ) or without (DOX − ) induction. Nuclei were stained with DAPI (blue) and SOX2-GFP (green). Scale bar, 10 µm. ( H ) Box and whisker plot quantifying normalized γH2AX intensity per nucleus from images in G (≥150 nuclei; three experiments). ( I ) Immunoblots of BL6 and FLAG-WT-SOX2 or FLAG-3A-SOX2 lines of cells synchronized with 10 µM RO3306 and then released for 0, 45, or 90 min. The dashed line represents where lanes were omitted. Blots were probed for SOX2, phospho-histone H3 (Ser10), γH2AX, and GAPDH.
    Figure Legend Snippet: Unconstrained mitotic SOX2 activity causes mitotic errors and genomic instability. ( A ) Volcano plot of bulk RNA-seq comparing 3A-SOX2 with WT-SOX2 after 72 h of 1 µg/mL doxycycline (DOX,) induction ( n = 3 replicates). Dashed lines mark P -value = 0.05 and log 2 fold change = 1. ( B ) Gene set enrichment analysis (DNA repair signatures) of the ranked gene list shown in A . ( C ) Time-lapse confocal images of WT-SOX2-GFP and 3A-SOX2-GFP mNSCs stained with SiRDNA (magenta) during mitosis; minutes after nuclear envelope breakdown are indicated. Scale bar, 10 µm. ( D ) Scatter plots (median ± IQR) of the time spent in prometaphase and telophase for WT versus 3A cells (15–20 mitoses; three experiments). ( E , left ) Representative DAPI images of a normal mitosis, lagging chromatin/anaphase bridge, and chromosome compaction defect. ( Right ) Stacked bar chart of event frequencies in parental BL6, WT-SOX2, and 3A-SOX2 lines (≥100 mitoses; three experiments). ( F ) Immunoblots of whole-cell lysates collected 0, 24, and 72 h after DOX induction ( top ) and probed for SOX2, GAPDH, and γH2AX. ( G ) Immunofluorescence for γH2AX (red) in BL6, WT-SOX2, and 3A-SOX2 cells with (DOX + ) or without (DOX − ) induction. Nuclei were stained with DAPI (blue) and SOX2-GFP (green). Scale bar, 10 µm. ( H ) Box and whisker plot quantifying normalized γH2AX intensity per nucleus from images in G (≥150 nuclei; three experiments). ( I ) Immunoblots of BL6 and FLAG-WT-SOX2 or FLAG-3A-SOX2 lines of cells synchronized with 10 µM RO3306 and then released for 0, 45, or 90 min. The dashed line represents where lanes were omitted. Blots were probed for SOX2, phospho-histone H3 (Ser10), γH2AX, and GAPDH.

    Techniques Used: Activity Assay, RNA Sequencing, Staining, Western Blot, Immunofluorescence, Whisker Assay



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    Unconstrained mitotic SOX2 activity causes mitotic errors and genomic instability. ( A ) Volcano plot of bulk RNA-seq comparing 3A-SOX2 with WT-SOX2 after 72 h of 1 µg/mL doxycycline (DOX,) induction ( n = 3 replicates). Dashed lines mark P -value = 0.05 and log 2 fold change = 1. ( B ) Gene set enrichment analysis (DNA repair signatures) of the ranked gene list shown in A . ( C ) Time-lapse confocal images of WT-SOX2-GFP and 3A-SOX2-GFP mNSCs stained with <t>SiRDNA</t> (magenta) during mitosis; minutes after nuclear envelope breakdown are indicated. Scale bar, 10 µm. ( D ) Scatter plots (median ± IQR) of the time spent in prometaphase and telophase for WT versus 3A cells (15–20 mitoses; three experiments). ( E , left ) <t>Representative</t> <t>DAPI</t> images of a normal mitosis, lagging chromatin/anaphase bridge, and chromosome compaction defect. ( Right ) Stacked bar chart of event frequencies in parental BL6, WT-SOX2, and 3A-SOX2 lines (≥100 mitoses; three experiments). ( F ) Immunoblots of whole-cell lysates collected 0, 24, and 72 h after DOX induction ( top ) and probed for SOX2, GAPDH, and γH2AX. ( G ) Immunofluorescence for γH2AX (red) in BL6, WT-SOX2, and 3A-SOX2 cells with (DOX + ) or without (DOX − ) induction. Nuclei were stained with DAPI (blue) and SOX2-GFP (green). Scale bar, 10 µm. ( H ) Box and whisker plot quantifying normalized γH2AX intensity per nucleus from images in G (≥150 nuclei; three experiments). ( I ) Immunoblots of BL6 and FLAG-WT-SOX2 or FLAG-3A-SOX2 lines of cells synchronized with 10 µM RO3306 and then released for 0, 45, or 90 min. The dashed line represents where lanes were omitted. Blots were probed for SOX2, phospho-histone H3 (Ser10), γH2AX, and GAPDH.
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    Image Search Results


    Unconstrained mitotic SOX2 activity causes mitotic errors and genomic instability. ( A ) Volcano plot of bulk RNA-seq comparing 3A-SOX2 with WT-SOX2 after 72 h of 1 µg/mL doxycycline (DOX,) induction ( n = 3 replicates). Dashed lines mark P -value = 0.05 and log 2 fold change = 1. ( B ) Gene set enrichment analysis (DNA repair signatures) of the ranked gene list shown in A . ( C ) Time-lapse confocal images of WT-SOX2-GFP and 3A-SOX2-GFP mNSCs stained with SiRDNA (magenta) during mitosis; minutes after nuclear envelope breakdown are indicated. Scale bar, 10 µm. ( D ) Scatter plots (median ± IQR) of the time spent in prometaphase and telophase for WT versus 3A cells (15–20 mitoses; three experiments). ( E , left ) Representative DAPI images of a normal mitosis, lagging chromatin/anaphase bridge, and chromosome compaction defect. ( Right ) Stacked bar chart of event frequencies in parental BL6, WT-SOX2, and 3A-SOX2 lines (≥100 mitoses; three experiments). ( F ) Immunoblots of whole-cell lysates collected 0, 24, and 72 h after DOX induction ( top ) and probed for SOX2, GAPDH, and γH2AX. ( G ) Immunofluorescence for γH2AX (red) in BL6, WT-SOX2, and 3A-SOX2 cells with (DOX + ) or without (DOX − ) induction. Nuclei were stained with DAPI (blue) and SOX2-GFP (green). Scale bar, 10 µm. ( H ) Box and whisker plot quantifying normalized γH2AX intensity per nucleus from images in G (≥150 nuclei; three experiments). ( I ) Immunoblots of BL6 and FLAG-WT-SOX2 or FLAG-3A-SOX2 lines of cells synchronized with 10 µM RO3306 and then released for 0, 45, or 90 min. The dashed line represents where lanes were omitted. Blots were probed for SOX2, phospho-histone H3 (Ser10), γH2AX, and GAPDH.

    Journal: Genes & Development

    Article Title: SOX2 phosphorylation during mitosis limits genomic damage

    doi: 10.1101/gad.352664.125

    Figure Lengend Snippet: Unconstrained mitotic SOX2 activity causes mitotic errors and genomic instability. ( A ) Volcano plot of bulk RNA-seq comparing 3A-SOX2 with WT-SOX2 after 72 h of 1 µg/mL doxycycline (DOX,) induction ( n = 3 replicates). Dashed lines mark P -value = 0.05 and log 2 fold change = 1. ( B ) Gene set enrichment analysis (DNA repair signatures) of the ranked gene list shown in A . ( C ) Time-lapse confocal images of WT-SOX2-GFP and 3A-SOX2-GFP mNSCs stained with SiRDNA (magenta) during mitosis; minutes after nuclear envelope breakdown are indicated. Scale bar, 10 µm. ( D ) Scatter plots (median ± IQR) of the time spent in prometaphase and telophase for WT versus 3A cells (15–20 mitoses; three experiments). ( E , left ) Representative DAPI images of a normal mitosis, lagging chromatin/anaphase bridge, and chromosome compaction defect. ( Right ) Stacked bar chart of event frequencies in parental BL6, WT-SOX2, and 3A-SOX2 lines (≥100 mitoses; three experiments). ( F ) Immunoblots of whole-cell lysates collected 0, 24, and 72 h after DOX induction ( top ) and probed for SOX2, GAPDH, and γH2AX. ( G ) Immunofluorescence for γH2AX (red) in BL6, WT-SOX2, and 3A-SOX2 cells with (DOX + ) or without (DOX − ) induction. Nuclei were stained with DAPI (blue) and SOX2-GFP (green). Scale bar, 10 µm. ( H ) Box and whisker plot quantifying normalized γH2AX intensity per nucleus from images in G (≥150 nuclei; three experiments). ( I ) Immunoblots of BL6 and FLAG-WT-SOX2 or FLAG-3A-SOX2 lines of cells synchronized with 10 µM RO3306 and then released for 0, 45, or 90 min. The dashed line represents where lanes were omitted. Blots were probed for SOX2, phospho-histone H3 (Ser10), γH2AX, and GAPDH.

    Article Snippet: Nuclei were counterstained with 1 μg/mL 4′,6′-diamidino-2-phenylindole (DAPI) or SiRDNA (Spirochrome) for 5 min.

    Techniques: Activity Assay, RNA Sequencing, Staining, Western Blot, Immunofluorescence, Whisker Assay