sil1  (Proteintech)

Journal: Nutrients

Article Title: Neuroprotective Effects of Desert Milk Exosomes in LPS-Induced Cognitive Decline: Role of Microglial M2 Polarization and AMPK Signaling

doi: 10.3390/nu18020315

Figure Lengend Snippet: Quality assessment of proteome data by label-free quantification. ( A ) Principal component analysis. ( B ) Hierarchical clustering analysis of differential protein expression profiles between D-Exo and ND-Exo. Red = Exosomes proteins with higher expression, green = Exosomes proteins with lower expression. ( C ) Difference expression pattern between D-Exo and ND-Exo. FDR = false discovery rate; FC = fold change. ( D ) The SIL1, FN1 protein with representative image by WB. ( E , F ) Expression of SIL1 and FN1 proteins in D-Exo and ND-Exo by WB method. Data are presented as mean ± SEM ( n = 3 per group). Statistically significant differences were indicated: * p < 0.05, ** p < 0.01, *** p < 0.001, ns > 0.05.

Article Snippet: Following blocking with 5% BSA, the membranes were incubated overnight at 4 °C with the primary antibody for FN1 (cat. #15613-1-AP, Proteintech, Shanghai, China), SIL1 (cat. #24110-1-AP, Proteintech, Shanghai, China), AMPK (cat. #10929-2-AP, Proteintech, Shanghai, China), phospho-AMPK (cat. #80209-6-RR, Proteintech, Shanghai, China), and GAPDH (cat. #10494-1-AP, Proteintech, Shanghai, China).

Techniques: Quantitative Proteomics, Expressing

Journal: Molecular Neurobiology

Article Title: Systematic Phenotyping and Molecular Analysis of the Woozy Mouse: A Preclinical Model of Cerebellar Ataxia

doi: 10.1007/s12035-025-05577-y

Figure Lengend Snippet: Progression of cerebellar damage shown over the weeks. Latency to fall in the overall population ( a ), male ( b ), female ( c ), Sil1 ht ( d ), and Sil1 wz ( e ) mice. Data are represented as scattered dot plots (mean ± SEM, of each group) and expressed as the percentage of the maximum time for the individual animal. Sil1 wz = green circles; Sil1 ht = blue circles. Significant differences are indicated (*p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.001)

Article Snippet: Sil1 homozygous (woozy) mice ( Sil1 wz ) were produced by crossing Sil1 heterozygous mice ( Sil1 ht ) (CXB5/By- Sil1 wz /J, JAX stock #003777), supplied by The Jackson Laboratory (Maine, USA).

Techniques:

Journal: Molecular Neurobiology

Article Title: Systematic Phenotyping and Molecular Analysis of the Woozy Mouse: A Preclinical Model of Cerebellar Ataxia

doi: 10.1007/s12035-025-05577-y

Figure Lengend Snippet: The beam walking test highlights the coordination problems manifested by the Sil1 wz mice. Time to traverse the bar and number of contralateral falls in the overall population ( a , d ), male ( b , e ), and female ( c , f ) mice. Data are represented as scattered dot plots (mean ± SEM, of each group). Sil1 wz = green circles; Sil1 ht = blue circles. Significant differences are indicated (*p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.001)

Article Snippet: Sil1 homozygous (woozy) mice ( Sil1 wz ) were produced by crossing Sil1 heterozygous mice ( Sil1 ht ) (CXB5/By- Sil1 wz /J, JAX stock #003777), supplied by The Jackson Laboratory (Maine, USA).

Techniques:

Journal: Molecular Neurobiology

Article Title: Systematic Phenotyping and Molecular Analysis of the Woozy Mouse: A Preclinical Model of Cerebellar Ataxia

doi: 10.1007/s12035-025-05577-y

Figure Lengend Snippet: Genotype- and sex-dependent impairments in the pole test manifested by the Sil1 wz mice. Turning (T-turn) and total (T-total) time at the 14 th and 16 th week of life in the overall population ( a ), male ( b ), female ( c ), Sil1 ht ( d ), and Sil1 wz ( e ) mice. Data are represented as scattered dot plots (mean ± SEM, of each group). Sil1 wz = green circles; Sil1 ht = blue circles; Males = blue circles; Females = red circles. Significant differences are indicated (*p < 0.05, **p < 0.01, ***p < 0.005)

Article Snippet: Sil1 homozygous (woozy) mice ( Sil1 wz ) were produced by crossing Sil1 heterozygous mice ( Sil1 ht ) (CXB5/By- Sil1 wz /J, JAX stock #003777), supplied by The Jackson Laboratory (Maine, USA).

Techniques:

Journal: Molecular Neurobiology

Article Title: Systematic Phenotyping and Molecular Analysis of the Woozy Mouse: A Preclinical Model of Cerebellar Ataxia

doi: 10.1007/s12035-025-05577-y

Figure Lengend Snippet: Muscular coordination impairment highlighted by the increased immobility in the Sil1 wz mice. Latency to fall, number of episodes, and immobility time for the individual parameter scored in the overall population ( a , d , g ), male ( b , e , h ), and female ( c , f , i ) mice. Data are represented as scattered dot plots (mean ± SEM, of each group). Sil1 wz = green circles; Sil1 ht = blue circles. Significant differences are indicated (*p < 0.05, ***p < 0.005)

Article Snippet: Sil1 homozygous (woozy) mice ( Sil1 wz ) were produced by crossing Sil1 heterozygous mice ( Sil1 ht ) (CXB5/By- Sil1 wz /J, JAX stock #003777), supplied by The Jackson Laboratory (Maine, USA).

Techniques:

Journal: Molecular Neurobiology

Article Title: Systematic Phenotyping and Molecular Analysis of the Woozy Mouse: A Preclinical Model of Cerebellar Ataxia

doi: 10.1007/s12035-025-05577-y

Figure Lengend Snippet: Genotype-specific correlation trend in the motor and cognitive tests. Spearman’s correlation analysis between the individual values of motor (Rotarod, Beam walking, Inverted screen, Pole) and cognitive (Nesting) tests considering the overall population ( a ), Sil1 ht ( b ), and Sil1 wz ( c ) mice. Cells filled in green to red gradient of the heat maps (upper part) represent Spearman’s r; cells filled in yellow to red gradient (lower part) represent p values (empty cells stand for p values greater than 0.05). The stars show the missed correlation between two parameters in the same test (blue) or in different tests (green) comparing the two experimental groups. BW = Beam Walking; IS = Inverted screen test; PT = Pole test; NB = Nesting building test

Article Snippet: Sil1 homozygous (woozy) mice ( Sil1 wz ) were produced by crossing Sil1 heterozygous mice ( Sil1 ht ) (CXB5/By- Sil1 wz /J, JAX stock #003777), supplied by The Jackson Laboratory (Maine, USA).

Techniques:

Journal: Molecular Neurobiology

Article Title: Systematic Phenotyping and Molecular Analysis of the Woozy Mouse: A Preclinical Model of Cerebellar Ataxia

doi: 10.1007/s12035-025-05577-y

Figure Lengend Snippet: Cross sectional area differences in the glycolytic but not in the oxidative muscles between the Sil1 wz and Sil1 ht mice. Hematoxylin & Eosin staining of gastrocnemius ( a and b ) and soleus ( d and e ) cross sections. Cross-sectional area (CSA—µm 2 ) of gastrocnemius ( c ) and soleus ( f ) muscular fibres measured in 26-week-old Sil1 ht and Sil1 wz mice. Data are represented as scattered dot plots (mean ± SEM, of each group). Sil1 wz = green circles; Sil1 ht = blue circles. Significant differences are indicated (****p < 0.001). Scale bar corresponds to 100 µm

Article Snippet: Sil1 homozygous (woozy) mice ( Sil1 wz ) were produced by crossing Sil1 heterozygous mice ( Sil1 ht ) (CXB5/By- Sil1 wz /J, JAX stock #003777), supplied by The Jackson Laboratory (Maine, USA).

Techniques: Muscles, Staining

Journal: Molecular Neurobiology

Article Title: Systematic Phenotyping and Molecular Analysis of the Woozy Mouse: A Preclinical Model of Cerebellar Ataxia

doi: 10.1007/s12035-025-05577-y

Figure Lengend Snippet: Increased levels of proteins associated with the unfolded protein response and involved in the proteolysis process in the Sil1 wz mice. Western Blot analysis performed in 26-week-old Sil1 ht and Sil1 wz mice ( a ). Protein fold change (relative to Gapdh) observed in the quadriceps ( b ) and soleus ( c ) of Sil1 ht and Sil1 wz mice. Significant differences are indicated (*p < 0.005, **p < 0.01)

Article Snippet: Sil1 homozygous (woozy) mice ( Sil1 wz ) were produced by crossing Sil1 heterozygous mice ( Sil1 ht ) (CXB5/By- Sil1 wz /J, JAX stock #003777), supplied by The Jackson Laboratory (Maine, USA).

Techniques: Western Blot