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squamous carcinoma siha cells  (ATCC)


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    Structured Review

    ATCC squamous carcinoma siha cells
    Squamous Carcinoma Siha Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 2231 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/siha/SiHa/pm42314419-63-7-14
    Average 98 stars, based on 2231 article reviews
    squamous carcinoma siha cells - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Identification of tumor-promoting functions of the Homeobox family transcription factor MSX1 in cervical cancer.
    Article Snippet: .. METHODS Cell culture, transfections and functional assays HeLa (ATCC ® CCL-2 TM) and SiHa (ATCC ® HTB-35 TM) cells were cultivated in Minimum Essential Medium (MEM) (Biowest) supplemented with 10% fetal bovine serum (Biowest), 1x penicillin/streptomycin (Gibco) at 37 °C with 5% CO2. .. The Sleeping Beauty Vector system was used to stably integrate the MSX1 gene (HeLa, SiHa) (VectorBuilder GmbH) or MSX1 tagged with FKBP12 (HeLa) (VectorBuilder GmbH) into the genome of the cells by the usage of a transposase (Supplementary Table S1).

    Article Title: Harnessing Id1 as a biomarker in a plasmid reporter system for cervical cancer.
    Article Snippet: .. Cell Culture Plasmid transfection and function was characterized in three cervical cancer cell lines: HeLa, CaSki (both provided by Dr. Nilam Sanjib Banerjee), and SiHa (American Type Culture Collection, Manassas, VA, USA). ..

    Transfection:

    Article Title: Identification of tumor-promoting functions of the Homeobox family transcription factor MSX1 in cervical cancer.
    Article Snippet: .. METHODS Cell culture, transfections and functional assays HeLa (ATCC ® CCL-2 TM) and SiHa (ATCC ® HTB-35 TM) cells were cultivated in Minimum Essential Medium (MEM) (Biowest) supplemented with 10% fetal bovine serum (Biowest), 1x penicillin/streptomycin (Gibco) at 37 °C with 5% CO2. .. The Sleeping Beauty Vector system was used to stably integrate the MSX1 gene (HeLa, SiHa) (VectorBuilder GmbH) or MSX1 tagged with FKBP12 (HeLa) (VectorBuilder GmbH) into the genome of the cells by the usage of a transposase (Supplementary Table S1).

    Article Title: Harnessing Id1 as a biomarker in a plasmid reporter system for cervical cancer.
    Article Snippet: .. Cell Culture Plasmid transfection and function was characterized in three cervical cancer cell lines: HeLa, CaSki (both provided by Dr. Nilam Sanjib Banerjee), and SiHa (American Type Culture Collection, Manassas, VA, USA). ..

    Functional Assay:

    Article Title: Identification of tumor-promoting functions of the Homeobox family transcription factor MSX1 in cervical cancer.
    Article Snippet: .. METHODS Cell culture, transfections and functional assays HeLa (ATCC ® CCL-2 TM) and SiHa (ATCC ® HTB-35 TM) cells were cultivated in Minimum Essential Medium (MEM) (Biowest) supplemented with 10% fetal bovine serum (Biowest), 1x penicillin/streptomycin (Gibco) at 37 °C with 5% CO2. .. The Sleeping Beauty Vector system was used to stably integrate the MSX1 gene (HeLa, SiHa) (VectorBuilder GmbH) or MSX1 tagged with FKBP12 (HeLa) (VectorBuilder GmbH) into the genome of the cells by the usage of a transposase (Supplementary Table S1).

    Plasmid Preparation:

    Article Title: Harnessing Id1 as a biomarker in a plasmid reporter system for cervical cancer.
    Article Snippet: .. Cell Culture Plasmid transfection and function was characterized in three cervical cancer cell lines: HeLa, CaSki (both provided by Dr. Nilam Sanjib Banerjee), and SiHa (American Type Culture Collection, Manassas, VA, USA). ..

    Derivative Assay:

    Article Title: Unlocking Biomedical Potential of the [Cu( l ‑isoleucine)(1,10-phenanthroline)(H 2 O)]Cl·2H 2 O Complex: A Comprehensive Study of Synthesis, Spectroscopic Analyses, Antitumor Efficacy, and Computational Insights
    Article Snippet: .. For the cytotoxicity assays, two cell lines were used: SiHa (HTB-35, ATCC, Manassas, VA), derived from human cervical epithelial carcinoma, and GM07492A (CVCL_7467, NIGMS), a nontumor human lung fibroblast cell line used as a normal control. .. SiHa and GM07492A cells were seeded in 96-well plates at densities of 1 × 10 4 cells/well (SiHa) and 2 × 10 4 cells/well (GM07492A) in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, Waltham, MA) supplemented with 10% fetal bovine serum (FBS; Gibco, Paisley, U.K.), amphotericin B (250 μg/mL), and penicillin/streptomycin (10,000 μg/mL).

    Control:

    Article Title: Unlocking Biomedical Potential of the [Cu( l ‑isoleucine)(1,10-phenanthroline)(H 2 O)]Cl·2H 2 O Complex: A Comprehensive Study of Synthesis, Spectroscopic Analyses, Antitumor Efficacy, and Computational Insights
    Article Snippet: .. For the cytotoxicity assays, two cell lines were used: SiHa (HTB-35, ATCC, Manassas, VA), derived from human cervical epithelial carcinoma, and GM07492A (CVCL_7467, NIGMS), a nontumor human lung fibroblast cell line used as a normal control. .. SiHa and GM07492A cells were seeded in 96-well plates at densities of 1 × 10 4 cells/well (SiHa) and 2 × 10 4 cells/well (GM07492A) in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, Waltham, MA) supplemented with 10% fetal bovine serum (FBS; Gibco, Paisley, U.K.), amphotericin B (250 μg/mL), and penicillin/streptomycin (10,000 μg/mL).

    Sulforhodamine B Assay:

    Article Title: Isoquinoline derivatives for use as antiviral and antitumour agents
    Article Snippet: Concentrations required for half maximal response of the compound for cell growth inhibition (EC50) is calculated by non-linear regression (log[conc. of inhibitor] versus response (% T/C)) using the analysis software GraphPad Prism®, Prism 5 for windows, version 5.01 (GraphPad Software Inc., CA). .. TABLE 8 SRB Assay Solutions SRB ASSAY SOLUTIONS SRB01 Fixing solution: trichloroacetic acid (TCA) 50% w/v in dH2O SRB02 Staining solution: Sulphorhodamine B (SRB) 0.4% w/v in SRB03 washing solution; SRB03 Washing solution: acetic acid 1% in dH2O SRB04 Developer solution: Tris-Base 10 mM TABLE 9 EC50 Measurements of cervical cancer cells in the presence of OC246 EC50 for OC246 was assessed for the HPV E7 expressing cervical cancer cell lines Hela (ATCC CCL-2), SiHa (ATCC HTB-35), and CaSki (ATCC CRM-CRL-1550) and compared to peripheral blood mononuclear cells (PBMC) in a SRB assay. ..

    Staining:

    Article Title: Isoquinoline derivatives for use as antiviral and antitumour agents
    Article Snippet: Concentrations required for half maximal response of the compound for cell growth inhibition (EC50) is calculated by non-linear regression (log[conc. of inhibitor] versus response (% T/C)) using the analysis software GraphPad Prism®, Prism 5 for windows, version 5.01 (GraphPad Software Inc., CA). .. TABLE 8 SRB Assay Solutions SRB ASSAY SOLUTIONS SRB01 Fixing solution: trichloroacetic acid (TCA) 50% w/v in dH2O SRB02 Staining solution: Sulphorhodamine B (SRB) 0.4% w/v in SRB03 washing solution; SRB03 Washing solution: acetic acid 1% in dH2O SRB04 Developer solution: Tris-Base 10 mM TABLE 9 EC50 Measurements of cervical cancer cells in the presence of OC246 EC50 for OC246 was assessed for the HPV E7 expressing cervical cancer cell lines Hela (ATCC CCL-2), SiHa (ATCC HTB-35), and CaSki (ATCC CRM-CRL-1550) and compared to peripheral blood mononuclear cells (PBMC) in a SRB assay. ..

    Expressing:

    Article Title: Isoquinoline derivatives for use as antiviral and antitumour agents
    Article Snippet: Concentrations required for half maximal response of the compound for cell growth inhibition (EC50) is calculated by non-linear regression (log[conc. of inhibitor] versus response (% T/C)) using the analysis software GraphPad Prism®, Prism 5 for windows, version 5.01 (GraphPad Software Inc., CA). .. TABLE 8 SRB Assay Solutions SRB ASSAY SOLUTIONS SRB01 Fixing solution: trichloroacetic acid (TCA) 50% w/v in dH2O SRB02 Staining solution: Sulphorhodamine B (SRB) 0.4% w/v in SRB03 washing solution; SRB03 Washing solution: acetic acid 1% in dH2O SRB04 Developer solution: Tris-Base 10 mM TABLE 9 EC50 Measurements of cervical cancer cells in the presence of OC246 EC50 for OC246 was assessed for the HPV E7 expressing cervical cancer cell lines Hela (ATCC CCL-2), SiHa (ATCC HTB-35), and CaSki (ATCC CRM-CRL-1550) and compared to peripheral blood mononuclear cells (PBMC) in a SRB assay. ..

    Fluorescence:

    Article Title: Development of a Telehealth-Enabled Portable Optical Endomicroscopy System with Targeted Peptides: A Preclinical Feasibility Study for Cervical Cancer Detection
    Article Snippet: .. To evaluate peptide-associated fluorescence, the following cell lines were used: (1) A549 (human lung carcinoma; American Type Culture Collection [ATCC] CCL-185 ), (2) SiHa (human squamous cell carcinoma of the cervix; ATCC HTB-35), and (3) CaSki (Carcinoma of the Sloan-Kettering Institute; ATCC CRL-1550 ). ..



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