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anti serca2  (Novus Biologicals)


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    Structured Review

    Novus Biologicals anti serca2
    Anti Serca2, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/serca2/SERCA2+ATPase+Antibody+(2A7-A1)/pm41896216-54-63-65
    Average 94 stars, based on 10 article reviews
    anti serca2 - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Control:

    Article Title: Ca 2+ tunneling architecture and function are important for secretion
    Article Snippet: Primary antibody incubation was performed at 4°C overnight.Primary antibody incubation was performed at 4°C overnight.. The following primary antibodies were used at a 1:500 dilution: SERCA2 (NB300-581; Novus Biologicals), STIM1 (5668S; Cell Signaling and MAI-19451; Thermo Fisher Scientific), KRAP (14157-1-AP; Thermo Fisher Scientific), NFAT1 (5861S; Cell Signaling), and ANO1 (14476; Cell Signaling).. For IP 3 R1 detection, a custom-made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used ( ).For IP 3 R1 detection, a custom-made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used ( ).

    Article Title: Ca2+ tunneling architecture and function are important for secretion.
    Article Snippet: Primary antibody incubation was performed at 4°C overnight.Primary antibody incubation was performed at 4°C overnight.. The following primary antibodies were used at a 1:500 dilution: SERCA2 (NB300-581; Novus Biologicals), STIM1 (5668S; Cell Signaling and MAI-19451; Thermo Fisher Scientific), KRAP (14157-1-AP; Thermo Fisher Scientific), NFAT1 (5861S; Cell Signaling), and ANO1 (14476; Cell Signaling).. For IP3R1 detection, a custom-made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used (Baker et al., 2021).For IP3R1 detection, a custom-made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used (Baker et al., 2021).

    Article Title: Architecture of Ca 2+ tunneling, a basic Ca 2+ signaling modality important for secretion
    Article Snippet: Primary antibody incubation was performed at 4°C overnight.Primary antibody incubation was performed at 4°C overnight.. The following primary antibodies were used at a 1:500 dilution: SERCA2 (NB300-581, Novus Biologicals), STIM1 (5668S, Cell Signaling and MAI-19451, Fisher), KRAP (14157-1-AP, Fisher), NFAT1 (5861S, Cell Signaling), ANO1 (14476, Cell Signaling).. For IP 3 R1 detection a custom made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used .For IP 3 R1 detection a custom made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used .

    Article Title: Amyloid Beta A4 Precursor Protein-binding Family B Member 1 (FE65) Interactomics Revealed Synaptic Vesicle Glycoprotein 2A (SV2A) and Sarcoplasmic/Endoplasmic Reticulum Calcium ATPase 2 (SERCA2) as New Binding Proteins in the Human Brain
    Article Snippet: In brief, magnetic beads (MB) (Invitrogen, Oslo, Norway) were covalently bound to either anti-mouse-Ig or anti-rabbit-Ig directed against the species-specific Fc part of the primary antibody.In brief, magnetic beads (MB) (Invitrogen, Oslo, Norway) were covalently bound to either anti-mouse-Ig or anti-rabbit-Ig directed against the species-specific Fc part of the primary antibody.. The following primary antibodies were used: FE65 (05–759, Millipore), GFP (sc 9996, Santa Cruz Biotechnology Darmstadt, Germany), SV2A (HPA007863, Sigma), and SERCA2 (NB-100–237, Novus Biologicals).. Cell extracts (500 μg of total protein) and 100 μl of MB were incubated together with a primary antibody (0.7 μg of antibody/50 μl of MB) for 48 h at 4 °C.Cell extracts (500 μg of total protein) and 100 μl of MB were incubated together with a primary antibody (0.7 μg of antibody/50 μl of MB) for 48 h at 4 °C.

    Article Title: Amyloid Beta A4 Precursor Protein-binding Family B Member 1 (FE65) Interactomics Revealed Synaptic Vesicle Glycoprotein 2A (SV2A) and Sarcoplasmic/Endoplasmic Reticulum Calcium ATPase 2 (SERCA2) as New Binding Proteins in the Human Brain
    Article Snippet: In brief, magnetic beads (MB) (Invitrogen, Oslo, Norway) were covalently bound to either anti-mouse-Ig or anti-rabbit-Ig directed against the species-specific Fc part of the primary antibody.In brief, magnetic beads (MB) (Invitrogen, Oslo, Norway) were covalently bound to either anti-mouse-Ig or anti-rabbit-Ig directed against the species-specific Fc part of the primary antibody.. The following primary antibodies were used: FE65 (05–759, Millipore), GFP (sc 9996, Santa Cruz Biotechnology Darmstadt, Germany), SV2A (HPA007863, Sigma), and SERCA2 (NB-100–237, Novus Biologicals).. Cell extracts (500 g of total protein) and 100 l of MB were incubated together with a primary antibody (0.7 g of antibody/50 l of MB) for 48 h at 4 °C.Cell extracts (500 g of total protein) and 100 l of MB were incubated together with a primary antibody (0.7 g of antibody/50 l of MB) for 48 h at 4 °C.

    Protein Binding:

    Article Title: Ca 2+ tunneling architecture and function are important for secretion
    Article Snippet: Primary antibody incubation was performed at 4°C overnight.Primary antibody incubation was performed at 4°C overnight.. The following primary antibodies were used at a 1:500 dilution: SERCA2 (NB300-581; Novus Biologicals), STIM1 (5668S; Cell Signaling and MAI-19451; Thermo Fisher Scientific), KRAP (14157-1-AP; Thermo Fisher Scientific), NFAT1 (5861S; Cell Signaling), and ANO1 (14476; Cell Signaling).. For IP 3 R1 detection, a custom-made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used ( ).For IP 3 R1 detection, a custom-made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used ( ).

    Article Title: Ca2+ tunneling architecture and function are important for secretion.
    Article Snippet: Primary antibody incubation was performed at 4°C overnight.Primary antibody incubation was performed at 4°C overnight.. The following primary antibodies were used at a 1:500 dilution: SERCA2 (NB300-581; Novus Biologicals), STIM1 (5668S; Cell Signaling and MAI-19451; Thermo Fisher Scientific), KRAP (14157-1-AP; Thermo Fisher Scientific), NFAT1 (5861S; Cell Signaling), and ANO1 (14476; Cell Signaling).. For IP3R1 detection, a custom-made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used (Baker et al., 2021).For IP3R1 detection, a custom-made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used (Baker et al., 2021).

    Article Title: Architecture of Ca 2+ tunneling, a basic Ca 2+ signaling modality important for secretion
    Article Snippet: Primary antibody incubation was performed at 4°C overnight.Primary antibody incubation was performed at 4°C overnight.. The following primary antibodies were used at a 1:500 dilution: SERCA2 (NB300-581, Novus Biologicals), STIM1 (5668S, Cell Signaling and MAI-19451, Fisher), KRAP (14157-1-AP, Fisher), NFAT1 (5861S, Cell Signaling), ANO1 (14476, Cell Signaling).. For IP 3 R1 detection a custom made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used .For IP 3 R1 detection a custom made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used .

    Article Title: Amyloid Beta A4 Precursor Protein-binding Family B Member 1 (FE65) Interactomics Revealed Synaptic Vesicle Glycoprotein 2A (SV2A) and Sarcoplasmic/Endoplasmic Reticulum Calcium ATPase 2 (SERCA2) as New Binding Proteins in the Human Brain
    Article Snippet: In brief, magnetic beads (MB) (Invitrogen, Oslo, Norway) were covalently bound to either anti-mouse-Ig or anti-rabbit-Ig directed against the species-specific Fc part of the primary antibody.In brief, magnetic beads (MB) (Invitrogen, Oslo, Norway) were covalently bound to either anti-mouse-Ig or anti-rabbit-Ig directed against the species-specific Fc part of the primary antibody.. The following primary antibodies were used: FE65 (05–759, Millipore), GFP (sc 9996, Santa Cruz Biotechnology Darmstadt, Germany), SV2A (HPA007863, Sigma), and SERCA2 (NB-100–237, Novus Biologicals).. Cell extracts (500 μg of total protein) and 100 μl of MB were incubated together with a primary antibody (0.7 μg of antibody/50 μl of MB) for 48 h at 4 °C.Cell extracts (500 μg of total protein) and 100 μl of MB were incubated together with a primary antibody (0.7 μg of antibody/50 μl of MB) for 48 h at 4 °C.

    Article Title: Amyloid Beta A4 Precursor Protein-binding Family B Member 1 (FE65) Interactomics Revealed Synaptic Vesicle Glycoprotein 2A (SV2A) and Sarcoplasmic/Endoplasmic Reticulum Calcium ATPase 2 (SERCA2) as New Binding Proteins in the Human Brain
    Article Snippet: In brief, magnetic beads (MB) (Invitrogen, Oslo, Norway) were covalently bound to either anti-mouse-Ig or anti-rabbit-Ig directed against the species-specific Fc part of the primary antibody.In brief, magnetic beads (MB) (Invitrogen, Oslo, Norway) were covalently bound to either anti-mouse-Ig or anti-rabbit-Ig directed against the species-specific Fc part of the primary antibody.. The following primary antibodies were used: FE65 (05–759, Millipore), GFP (sc 9996, Santa Cruz Biotechnology Darmstadt, Germany), SV2A (HPA007863, Sigma), and SERCA2 (NB-100–237, Novus Biologicals).. Cell extracts (500 g of total protein) and 100 l of MB were incubated together with a primary antibody (0.7 g of antibody/50 l of MB) for 48 h at 4 °C.Cell extracts (500 g of total protein) and 100 l of MB were incubated together with a primary antibody (0.7 g of antibody/50 l of MB) for 48 h at 4 °C.

    Clinical Proteomics:

    Article Title: Ca 2+ tunneling architecture and function are important for secretion
    Article Snippet: Primary antibody incubation was performed at 4°C overnight.Primary antibody incubation was performed at 4°C overnight.. The following primary antibodies were used at a 1:500 dilution: SERCA2 (NB300-581; Novus Biologicals), STIM1 (5668S; Cell Signaling and MAI-19451; Thermo Fisher Scientific), KRAP (14157-1-AP; Thermo Fisher Scientific), NFAT1 (5861S; Cell Signaling), and ANO1 (14476; Cell Signaling).. For IP 3 R1 detection, a custom-made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used ( ).For IP 3 R1 detection, a custom-made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used ( ).

    Article Title: Ca2+ tunneling architecture and function are important for secretion.
    Article Snippet: Primary antibody incubation was performed at 4°C overnight.Primary antibody incubation was performed at 4°C overnight.. The following primary antibodies were used at a 1:500 dilution: SERCA2 (NB300-581; Novus Biologicals), STIM1 (5668S; Cell Signaling and MAI-19451; Thermo Fisher Scientific), KRAP (14157-1-AP; Thermo Fisher Scientific), NFAT1 (5861S; Cell Signaling), and ANO1 (14476; Cell Signaling).. For IP3R1 detection, a custom-made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used (Baker et al., 2021).For IP3R1 detection, a custom-made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used (Baker et al., 2021).

    Article Title: Architecture of Ca 2+ tunneling, a basic Ca 2+ signaling modality important for secretion
    Article Snippet: Primary antibody incubation was performed at 4°C overnight.Primary antibody incubation was performed at 4°C overnight.. The following primary antibodies were used at a 1:500 dilution: SERCA2 (NB300-581, Novus Biologicals), STIM1 (5668S, Cell Signaling and MAI-19451, Fisher), KRAP (14157-1-AP, Fisher), NFAT1 (5861S, Cell Signaling), ANO1 (14476, Cell Signaling).. For IP 3 R1 detection a custom made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used .For IP 3 R1 detection a custom made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used .

    Article Title: Amyloid Beta A4 Precursor Protein-binding Family B Member 1 (FE65) Interactomics Revealed Synaptic Vesicle Glycoprotein 2A (SV2A) and Sarcoplasmic/Endoplasmic Reticulum Calcium ATPase 2 (SERCA2) as New Binding Proteins in the Human Brain
    Article Snippet: In brief, magnetic beads (MB) (Invitrogen, Oslo, Norway) were covalently bound to either anti-mouse-Ig or anti-rabbit-Ig directed against the species-specific Fc part of the primary antibody.In brief, magnetic beads (MB) (Invitrogen, Oslo, Norway) were covalently bound to either anti-mouse-Ig or anti-rabbit-Ig directed against the species-specific Fc part of the primary antibody.. The following primary antibodies were used: FE65 (05–759, Millipore), GFP (sc 9996, Santa Cruz Biotechnology Darmstadt, Germany), SV2A (HPA007863, Sigma), and SERCA2 (NB-100–237, Novus Biologicals).. Cell extracts (500 μg of total protein) and 100 μl of MB were incubated together with a primary antibody (0.7 μg of antibody/50 μl of MB) for 48 h at 4 °C.Cell extracts (500 μg of total protein) and 100 μl of MB were incubated together with a primary antibody (0.7 μg of antibody/50 μl of MB) for 48 h at 4 °C.

    Article Title: Amyloid Beta A4 Precursor Protein-binding Family B Member 1 (FE65) Interactomics Revealed Synaptic Vesicle Glycoprotein 2A (SV2A) and Sarcoplasmic/Endoplasmic Reticulum Calcium ATPase 2 (SERCA2) as New Binding Proteins in the Human Brain
    Article Snippet: In brief, magnetic beads (MB) (Invitrogen, Oslo, Norway) were covalently bound to either anti-mouse-Ig or anti-rabbit-Ig directed against the species-specific Fc part of the primary antibody.In brief, magnetic beads (MB) (Invitrogen, Oslo, Norway) were covalently bound to either anti-mouse-Ig or anti-rabbit-Ig directed against the species-specific Fc part of the primary antibody.. The following primary antibodies were used: FE65 (05–759, Millipore), GFP (sc 9996, Santa Cruz Biotechnology Darmstadt, Germany), SV2A (HPA007863, Sigma), and SERCA2 (NB-100–237, Novus Biologicals).. Cell extracts (500 g of total protein) and 100 l of MB were incubated together with a primary antibody (0.7 g of antibody/50 l of MB) for 48 h at 4 °C.Cell extracts (500 g of total protein) and 100 l of MB were incubated together with a primary antibody (0.7 g of antibody/50 l of MB) for 48 h at 4 °C.

    Membrane:

    Article Title: Ca 2+ tunneling architecture and function are important for secretion
    Article Snippet: Primary antibody incubation was performed at 4°C overnight.Primary antibody incubation was performed at 4°C overnight.. The following primary antibodies were used at a 1:500 dilution: SERCA2 (NB300-581; Novus Biologicals), STIM1 (5668S; Cell Signaling and MAI-19451; Thermo Fisher Scientific), KRAP (14157-1-AP; Thermo Fisher Scientific), NFAT1 (5861S; Cell Signaling), and ANO1 (14476; Cell Signaling).. For IP 3 R1 detection, a custom-made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used ( ).For IP 3 R1 detection, a custom-made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used ( ).

    Article Title: Ca2+ tunneling architecture and function are important for secretion.
    Article Snippet: Primary antibody incubation was performed at 4°C overnight.Primary antibody incubation was performed at 4°C overnight.. The following primary antibodies were used at a 1:500 dilution: SERCA2 (NB300-581; Novus Biologicals), STIM1 (5668S; Cell Signaling and MAI-19451; Thermo Fisher Scientific), KRAP (14157-1-AP; Thermo Fisher Scientific), NFAT1 (5861S; Cell Signaling), and ANO1 (14476; Cell Signaling).. For IP3R1 detection, a custom-made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used (Baker et al., 2021).For IP3R1 detection, a custom-made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used (Baker et al., 2021).

    Article Title: Architecture of Ca 2+ tunneling, a basic Ca 2+ signaling modality important for secretion
    Article Snippet: Primary antibody incubation was performed at 4°C overnight.Primary antibody incubation was performed at 4°C overnight.. The following primary antibodies were used at a 1:500 dilution: SERCA2 (NB300-581, Novus Biologicals), STIM1 (5668S, Cell Signaling and MAI-19451, Fisher), KRAP (14157-1-AP, Fisher), NFAT1 (5861S, Cell Signaling), ANO1 (14476, Cell Signaling).. For IP 3 R1 detection a custom made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used .For IP 3 R1 detection a custom made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used .

    Article Title: Amyloid Beta A4 Precursor Protein-binding Family B Member 1 (FE65) Interactomics Revealed Synaptic Vesicle Glycoprotein 2A (SV2A) and Sarcoplasmic/Endoplasmic Reticulum Calcium ATPase 2 (SERCA2) as New Binding Proteins in the Human Brain
    Article Snippet: In brief, magnetic beads (MB) (Invitrogen, Oslo, Norway) were covalently bound to either anti-mouse-Ig or anti-rabbit-Ig directed against the species-specific Fc part of the primary antibody.In brief, magnetic beads (MB) (Invitrogen, Oslo, Norway) were covalently bound to either anti-mouse-Ig or anti-rabbit-Ig directed against the species-specific Fc part of the primary antibody.. The following primary antibodies were used: FE65 (05–759, Millipore), GFP (sc 9996, Santa Cruz Biotechnology Darmstadt, Germany), SV2A (HPA007863, Sigma), and SERCA2 (NB-100–237, Novus Biologicals).. Cell extracts (500 μg of total protein) and 100 μl of MB were incubated together with a primary antibody (0.7 μg of antibody/50 μl of MB) for 48 h at 4 °C.Cell extracts (500 μg of total protein) and 100 μl of MB were incubated together with a primary antibody (0.7 μg of antibody/50 μl of MB) for 48 h at 4 °C.

    Article Title: Amyloid Beta A4 Precursor Protein-binding Family B Member 1 (FE65) Interactomics Revealed Synaptic Vesicle Glycoprotein 2A (SV2A) and Sarcoplasmic/Endoplasmic Reticulum Calcium ATPase 2 (SERCA2) as New Binding Proteins in the Human Brain
    Article Snippet: In brief, magnetic beads (MB) (Invitrogen, Oslo, Norway) were covalently bound to either anti-mouse-Ig or anti-rabbit-Ig directed against the species-specific Fc part of the primary antibody.In brief, magnetic beads (MB) (Invitrogen, Oslo, Norway) were covalently bound to either anti-mouse-Ig or anti-rabbit-Ig directed against the species-specific Fc part of the primary antibody.. The following primary antibodies were used: FE65 (05–759, Millipore), GFP (sc 9996, Santa Cruz Biotechnology Darmstadt, Germany), SV2A (HPA007863, Sigma), and SERCA2 (NB-100–237, Novus Biologicals).. Cell extracts (500 g of total protein) and 100 l of MB were incubated together with a primary antibody (0.7 g of antibody/50 l of MB) for 48 h at 4 °C.Cell extracts (500 g of total protein) and 100 l of MB were incubated together with a primary antibody (0.7 g of antibody/50 l of MB) for 48 h at 4 °C.

    Coagulation:

    Article Title: Ca 2+ tunneling architecture and function are important for secretion
    Article Snippet: Primary antibody incubation was performed at 4°C overnight.Primary antibody incubation was performed at 4°C overnight.. The following primary antibodies were used at a 1:500 dilution: SERCA2 (NB300-581; Novus Biologicals), STIM1 (5668S; Cell Signaling and MAI-19451; Thermo Fisher Scientific), KRAP (14157-1-AP; Thermo Fisher Scientific), NFAT1 (5861S; Cell Signaling), and ANO1 (14476; Cell Signaling).. For IP 3 R1 detection, a custom-made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used ( ).For IP 3 R1 detection, a custom-made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used ( ).

    Article Title: Ca2+ tunneling architecture and function are important for secretion.
    Article Snippet: Primary antibody incubation was performed at 4°C overnight.Primary antibody incubation was performed at 4°C overnight.. The following primary antibodies were used at a 1:500 dilution: SERCA2 (NB300-581; Novus Biologicals), STIM1 (5668S; Cell Signaling and MAI-19451; Thermo Fisher Scientific), KRAP (14157-1-AP; Thermo Fisher Scientific), NFAT1 (5861S; Cell Signaling), and ANO1 (14476; Cell Signaling).. For IP3R1 detection, a custom-made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used (Baker et al., 2021).For IP3R1 detection, a custom-made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used (Baker et al., 2021).

    Article Title: Architecture of Ca 2+ tunneling, a basic Ca 2+ signaling modality important for secretion
    Article Snippet: Primary antibody incubation was performed at 4°C overnight.Primary antibody incubation was performed at 4°C overnight.. The following primary antibodies were used at a 1:500 dilution: SERCA2 (NB300-581, Novus Biologicals), STIM1 (5668S, Cell Signaling and MAI-19451, Fisher), KRAP (14157-1-AP, Fisher), NFAT1 (5861S, Cell Signaling), ANO1 (14476, Cell Signaling).. For IP 3 R1 detection a custom made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used .For IP 3 R1 detection a custom made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used .

    Article Title: Amyloid Beta A4 Precursor Protein-binding Family B Member 1 (FE65) Interactomics Revealed Synaptic Vesicle Glycoprotein 2A (SV2A) and Sarcoplasmic/Endoplasmic Reticulum Calcium ATPase 2 (SERCA2) as New Binding Proteins in the Human Brain
    Article Snippet: In brief, magnetic beads (MB) (Invitrogen, Oslo, Norway) were covalently bound to either anti-mouse-Ig or anti-rabbit-Ig directed against the species-specific Fc part of the primary antibody.In brief, magnetic beads (MB) (Invitrogen, Oslo, Norway) were covalently bound to either anti-mouse-Ig or anti-rabbit-Ig directed against the species-specific Fc part of the primary antibody.. The following primary antibodies were used: FE65 (05–759, Millipore), GFP (sc 9996, Santa Cruz Biotechnology Darmstadt, Germany), SV2A (HPA007863, Sigma), and SERCA2 (NB-100–237, Novus Biologicals).. Cell extracts (500 μg of total protein) and 100 μl of MB were incubated together with a primary antibody (0.7 μg of antibody/50 μl of MB) for 48 h at 4 °C.Cell extracts (500 μg of total protein) and 100 μl of MB were incubated together with a primary antibody (0.7 μg of antibody/50 μl of MB) for 48 h at 4 °C.

    Article Title: Amyloid Beta A4 Precursor Protein-binding Family B Member 1 (FE65) Interactomics Revealed Synaptic Vesicle Glycoprotein 2A (SV2A) and Sarcoplasmic/Endoplasmic Reticulum Calcium ATPase 2 (SERCA2) as New Binding Proteins in the Human Brain
    Article Snippet: In brief, magnetic beads (MB) (Invitrogen, Oslo, Norway) were covalently bound to either anti-mouse-Ig or anti-rabbit-Ig directed against the species-specific Fc part of the primary antibody.In brief, magnetic beads (MB) (Invitrogen, Oslo, Norway) were covalently bound to either anti-mouse-Ig or anti-rabbit-Ig directed against the species-specific Fc part of the primary antibody.. The following primary antibodies were used: FE65 (05–759, Millipore), GFP (sc 9996, Santa Cruz Biotechnology Darmstadt, Germany), SV2A (HPA007863, Sigma), and SERCA2 (NB-100–237, Novus Biologicals).. Cell extracts (500 g of total protein) and 100 l of MB were incubated together with a primary antibody (0.7 g of antibody/50 l of MB) for 48 h at 4 °C.Cell extracts (500 g of total protein) and 100 l of MB were incubated together with a primary antibody (0.7 g of antibody/50 l of MB) for 48 h at 4 °C.

    Biomarker Discovery:

    Article Title: Ca 2+ tunneling architecture and function are important for secretion
    Article Snippet: Primary antibody incubation was performed at 4°C overnight.Primary antibody incubation was performed at 4°C overnight.. The following primary antibodies were used at a 1:500 dilution: SERCA2 (NB300-581; Novus Biologicals), STIM1 (5668S; Cell Signaling and MAI-19451; Thermo Fisher Scientific), KRAP (14157-1-AP; Thermo Fisher Scientific), NFAT1 (5861S; Cell Signaling), and ANO1 (14476; Cell Signaling).. For IP 3 R1 detection, a custom-made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used ( ).For IP 3 R1 detection, a custom-made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used ( ).

    Article Title: Ca2+ tunneling architecture and function are important for secretion.
    Article Snippet: Primary antibody incubation was performed at 4°C overnight.Primary antibody incubation was performed at 4°C overnight.. The following primary antibodies were used at a 1:500 dilution: SERCA2 (NB300-581; Novus Biologicals), STIM1 (5668S; Cell Signaling and MAI-19451; Thermo Fisher Scientific), KRAP (14157-1-AP; Thermo Fisher Scientific), NFAT1 (5861S; Cell Signaling), and ANO1 (14476; Cell Signaling).. For IP3R1 detection, a custom-made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used (Baker et al., 2021).For IP3R1 detection, a custom-made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used (Baker et al., 2021).

    Article Title: Architecture of Ca 2+ tunneling, a basic Ca 2+ signaling modality important for secretion
    Article Snippet: Primary antibody incubation was performed at 4°C overnight.Primary antibody incubation was performed at 4°C overnight.. The following primary antibodies were used at a 1:500 dilution: SERCA2 (NB300-581, Novus Biologicals), STIM1 (5668S, Cell Signaling and MAI-19451, Fisher), KRAP (14157-1-AP, Fisher), NFAT1 (5861S, Cell Signaling), ANO1 (14476, Cell Signaling).. For IP 3 R1 detection a custom made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used .For IP 3 R1 detection a custom made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used .

    Article Title: Amyloid Beta A4 Precursor Protein-binding Family B Member 1 (FE65) Interactomics Revealed Synaptic Vesicle Glycoprotein 2A (SV2A) and Sarcoplasmic/Endoplasmic Reticulum Calcium ATPase 2 (SERCA2) as New Binding Proteins in the Human Brain
    Article Snippet: In brief, magnetic beads (MB) (Invitrogen, Oslo, Norway) were covalently bound to either anti-mouse-Ig or anti-rabbit-Ig directed against the species-specific Fc part of the primary antibody.In brief, magnetic beads (MB) (Invitrogen, Oslo, Norway) were covalently bound to either anti-mouse-Ig or anti-rabbit-Ig directed against the species-specific Fc part of the primary antibody.. The following primary antibodies were used: FE65 (05–759, Millipore), GFP (sc 9996, Santa Cruz Biotechnology Darmstadt, Germany), SV2A (HPA007863, Sigma), and SERCA2 (NB-100–237, Novus Biologicals).. Cell extracts (500 μg of total protein) and 100 μl of MB were incubated together with a primary antibody (0.7 μg of antibody/50 μl of MB) for 48 h at 4 °C.Cell extracts (500 μg of total protein) and 100 μl of MB were incubated together with a primary antibody (0.7 μg of antibody/50 μl of MB) for 48 h at 4 °C.

    Article Title: Amyloid Beta A4 Precursor Protein-binding Family B Member 1 (FE65) Interactomics Revealed Synaptic Vesicle Glycoprotein 2A (SV2A) and Sarcoplasmic/Endoplasmic Reticulum Calcium ATPase 2 (SERCA2) as New Binding Proteins in the Human Brain
    Article Snippet: In brief, magnetic beads (MB) (Invitrogen, Oslo, Norway) were covalently bound to either anti-mouse-Ig or anti-rabbit-Ig directed against the species-specific Fc part of the primary antibody.In brief, magnetic beads (MB) (Invitrogen, Oslo, Norway) were covalently bound to either anti-mouse-Ig or anti-rabbit-Ig directed against the species-specific Fc part of the primary antibody.. The following primary antibodies were used: FE65 (05–759, Millipore), GFP (sc 9996, Santa Cruz Biotechnology Darmstadt, Germany), SV2A (HPA007863, Sigma), and SERCA2 (NB-100–237, Novus Biologicals).. Cell extracts (500 g of total protein) and 100 l of MB were incubated together with a primary antibody (0.7 g of antibody/50 l of MB) for 48 h at 4 °C.Cell extracts (500 g of total protein) and 100 l of MB were incubated together with a primary antibody (0.7 g of antibody/50 l of MB) for 48 h at 4 °C.

    Western Blot:

    Article Title: Ca 2+ tunneling architecture and function are important for secretion
    Article Snippet: Primary antibody incubation was performed at 4°C overnight.Primary antibody incubation was performed at 4°C overnight.. The following primary antibodies were used at a 1:500 dilution: SERCA2 (NB300-581; Novus Biologicals), STIM1 (5668S; Cell Signaling and MAI-19451; Thermo Fisher Scientific), KRAP (14157-1-AP; Thermo Fisher Scientific), NFAT1 (5861S; Cell Signaling), and ANO1 (14476; Cell Signaling).. For IP 3 R1 detection, a custom-made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used ( ).For IP 3 R1 detection, a custom-made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used ( ).

    Article Title: Ca2+ tunneling architecture and function are important for secretion.
    Article Snippet: Primary antibody incubation was performed at 4°C overnight.Primary antibody incubation was performed at 4°C overnight.. The following primary antibodies were used at a 1:500 dilution: SERCA2 (NB300-581; Novus Biologicals), STIM1 (5668S; Cell Signaling and MAI-19451; Thermo Fisher Scientific), KRAP (14157-1-AP; Thermo Fisher Scientific), NFAT1 (5861S; Cell Signaling), and ANO1 (14476; Cell Signaling).. For IP3R1 detection, a custom-made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used (Baker et al., 2021).For IP3R1 detection, a custom-made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used (Baker et al., 2021).

    Article Title: Architecture of Ca 2+ tunneling, a basic Ca 2+ signaling modality important for secretion
    Article Snippet: Primary antibody incubation was performed at 4°C overnight.Primary antibody incubation was performed at 4°C overnight.. The following primary antibodies were used at a 1:500 dilution: SERCA2 (NB300-581, Novus Biologicals), STIM1 (5668S, Cell Signaling and MAI-19451, Fisher), KRAP (14157-1-AP, Fisher), NFAT1 (5861S, Cell Signaling), ANO1 (14476, Cell Signaling).. For IP 3 R1 detection a custom made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used .For IP 3 R1 detection a custom made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used .

    Article Title: Amyloid Beta A4 Precursor Protein-binding Family B Member 1 (FE65) Interactomics Revealed Synaptic Vesicle Glycoprotein 2A (SV2A) and Sarcoplasmic/Endoplasmic Reticulum Calcium ATPase 2 (SERCA2) as New Binding Proteins in the Human Brain
    Article Snippet: In brief, magnetic beads (MB) (Invitrogen, Oslo, Norway) were covalently bound to either anti-mouse-Ig or anti-rabbit-Ig directed against the species-specific Fc part of the primary antibody.In brief, magnetic beads (MB) (Invitrogen, Oslo, Norway) were covalently bound to either anti-mouse-Ig or anti-rabbit-Ig directed against the species-specific Fc part of the primary antibody.. The following primary antibodies were used: FE65 (05–759, Millipore), GFP (sc 9996, Santa Cruz Biotechnology Darmstadt, Germany), SV2A (HPA007863, Sigma), and SERCA2 (NB-100–237, Novus Biologicals).. Cell extracts (500 μg of total protein) and 100 μl of MB were incubated together with a primary antibody (0.7 μg of antibody/50 μl of MB) for 48 h at 4 °C.Cell extracts (500 μg of total protein) and 100 μl of MB were incubated together with a primary antibody (0.7 μg of antibody/50 μl of MB) for 48 h at 4 °C.

    Article Title: Amyloid Beta A4 Precursor Protein-binding Family B Member 1 (FE65) Interactomics Revealed Synaptic Vesicle Glycoprotein 2A (SV2A) and Sarcoplasmic/Endoplasmic Reticulum Calcium ATPase 2 (SERCA2) as New Binding Proteins in the Human Brain
    Article Snippet: In brief, magnetic beads (MB) (Invitrogen, Oslo, Norway) were covalently bound to either anti-mouse-Ig or anti-rabbit-Ig directed against the species-specific Fc part of the primary antibody.In brief, magnetic beads (MB) (Invitrogen, Oslo, Norway) were covalently bound to either anti-mouse-Ig or anti-rabbit-Ig directed against the species-specific Fc part of the primary antibody.. The following primary antibodies were used: FE65 (05–759, Millipore), GFP (sc 9996, Santa Cruz Biotechnology Darmstadt, Germany), SV2A (HPA007863, Sigma), and SERCA2 (NB-100–237, Novus Biologicals).. Cell extracts (500 g of total protein) and 100 l of MB were incubated together with a primary antibody (0.7 g of antibody/50 l of MB) for 48 h at 4 °C.Cell extracts (500 g of total protein) and 100 l of MB were incubated together with a primary antibody (0.7 g of antibody/50 l of MB) for 48 h at 4 °C.

    Knockdown:

    Article Title: Ca 2+ tunneling architecture and function are important for secretion
    Article Snippet: Primary antibody incubation was performed at 4°C overnight.Primary antibody incubation was performed at 4°C overnight.. The following primary antibodies were used at a 1:500 dilution: SERCA2 (NB300-581; Novus Biologicals), STIM1 (5668S; Cell Signaling and MAI-19451; Thermo Fisher Scientific), KRAP (14157-1-AP; Thermo Fisher Scientific), NFAT1 (5861S; Cell Signaling), and ANO1 (14476; Cell Signaling).. For IP 3 R1 detection, a custom-made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used ( ).For IP 3 R1 detection, a custom-made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used ( ).

    Article Title: Ca2+ tunneling architecture and function are important for secretion.
    Article Snippet: Primary antibody incubation was performed at 4°C overnight.Primary antibody incubation was performed at 4°C overnight.. The following primary antibodies were used at a 1:500 dilution: SERCA2 (NB300-581; Novus Biologicals), STIM1 (5668S; Cell Signaling and MAI-19451; Thermo Fisher Scientific), KRAP (14157-1-AP; Thermo Fisher Scientific), NFAT1 (5861S; Cell Signaling), and ANO1 (14476; Cell Signaling).. For IP3R1 detection, a custom-made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used (Baker et al., 2021).For IP3R1 detection, a custom-made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used (Baker et al., 2021).

    Article Title: Architecture of Ca 2+ tunneling, a basic Ca 2+ signaling modality important for secretion
    Article Snippet: Primary antibody incubation was performed at 4°C overnight.Primary antibody incubation was performed at 4°C overnight.. The following primary antibodies were used at a 1:500 dilution: SERCA2 (NB300-581, Novus Biologicals), STIM1 (5668S, Cell Signaling and MAI-19451, Fisher), KRAP (14157-1-AP, Fisher), NFAT1 (5861S, Cell Signaling), ANO1 (14476, Cell Signaling).. For IP 3 R1 detection a custom made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used .For IP 3 R1 detection a custom made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used .

    Article Title: Amyloid Beta A4 Precursor Protein-binding Family B Member 1 (FE65) Interactomics Revealed Synaptic Vesicle Glycoprotein 2A (SV2A) and Sarcoplasmic/Endoplasmic Reticulum Calcium ATPase 2 (SERCA2) as New Binding Proteins in the Human Brain
    Article Snippet: In brief, magnetic beads (MB) (Invitrogen, Oslo, Norway) were covalently bound to either anti-mouse-Ig or anti-rabbit-Ig directed against the species-specific Fc part of the primary antibody.In brief, magnetic beads (MB) (Invitrogen, Oslo, Norway) were covalently bound to either anti-mouse-Ig or anti-rabbit-Ig directed against the species-specific Fc part of the primary antibody.. The following primary antibodies were used: FE65 (05–759, Millipore), GFP (sc 9996, Santa Cruz Biotechnology Darmstadt, Germany), SV2A (HPA007863, Sigma), and SERCA2 (NB-100–237, Novus Biologicals).. Cell extracts (500 μg of total protein) and 100 μl of MB were incubated together with a primary antibody (0.7 μg of antibody/50 μl of MB) for 48 h at 4 °C.Cell extracts (500 μg of total protein) and 100 μl of MB were incubated together with a primary antibody (0.7 μg of antibody/50 μl of MB) for 48 h at 4 °C.

    Article Title: Amyloid Beta A4 Precursor Protein-binding Family B Member 1 (FE65) Interactomics Revealed Synaptic Vesicle Glycoprotein 2A (SV2A) and Sarcoplasmic/Endoplasmic Reticulum Calcium ATPase 2 (SERCA2) as New Binding Proteins in the Human Brain
    Article Snippet: In brief, magnetic beads (MB) (Invitrogen, Oslo, Norway) were covalently bound to either anti-mouse-Ig or anti-rabbit-Ig directed against the species-specific Fc part of the primary antibody.In brief, magnetic beads (MB) (Invitrogen, Oslo, Norway) were covalently bound to either anti-mouse-Ig or anti-rabbit-Ig directed against the species-specific Fc part of the primary antibody.. The following primary antibodies were used: FE65 (05–759, Millipore), GFP (sc 9996, Santa Cruz Biotechnology Darmstadt, Germany), SV2A (HPA007863, Sigma), and SERCA2 (NB-100–237, Novus Biologicals).. Cell extracts (500 g of total protein) and 100 l of MB were incubated together with a primary antibody (0.7 g of antibody/50 l of MB) for 48 h at 4 °C.Cell extracts (500 g of total protein) and 100 l of MB were incubated together with a primary antibody (0.7 g of antibody/50 l of MB) for 48 h at 4 °C.

    Immunoprecipitation:

    Article Title: Ca 2+ tunneling architecture and function are important for secretion
    Article Snippet: Primary antibody incubation was performed at 4°C overnight.Primary antibody incubation was performed at 4°C overnight.. The following primary antibodies were used at a 1:500 dilution: SERCA2 (NB300-581; Novus Biologicals), STIM1 (5668S; Cell Signaling and MAI-19451; Thermo Fisher Scientific), KRAP (14157-1-AP; Thermo Fisher Scientific), NFAT1 (5861S; Cell Signaling), and ANO1 (14476; Cell Signaling).. For IP 3 R1 detection, a custom-made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used ( ).For IP 3 R1 detection, a custom-made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used ( ).

    Article Title: Ca2+ tunneling architecture and function are important for secretion.
    Article Snippet: Primary antibody incubation was performed at 4°C overnight.Primary antibody incubation was performed at 4°C overnight.. The following primary antibodies were used at a 1:500 dilution: SERCA2 (NB300-581; Novus Biologicals), STIM1 (5668S; Cell Signaling and MAI-19451; Thermo Fisher Scientific), KRAP (14157-1-AP; Thermo Fisher Scientific), NFAT1 (5861S; Cell Signaling), and ANO1 (14476; Cell Signaling).. For IP3R1 detection, a custom-made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used (Baker et al., 2021).For IP3R1 detection, a custom-made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used (Baker et al., 2021).

    Article Title: Architecture of Ca 2+ tunneling, a basic Ca 2+ signaling modality important for secretion
    Article Snippet: Primary antibody incubation was performed at 4°C overnight.Primary antibody incubation was performed at 4°C overnight.. The following primary antibodies were used at a 1:500 dilution: SERCA2 (NB300-581, Novus Biologicals), STIM1 (5668S, Cell Signaling and MAI-19451, Fisher), KRAP (14157-1-AP, Fisher), NFAT1 (5861S, Cell Signaling), ANO1 (14476, Cell Signaling).. For IP 3 R1 detection a custom made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used .For IP 3 R1 detection a custom made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used .

    Article Title: Amyloid Beta A4 Precursor Protein-binding Family B Member 1 (FE65) Interactomics Revealed Synaptic Vesicle Glycoprotein 2A (SV2A) and Sarcoplasmic/Endoplasmic Reticulum Calcium ATPase 2 (SERCA2) as New Binding Proteins in the Human Brain
    Article Snippet: In brief, magnetic beads (MB) (Invitrogen, Oslo, Norway) were covalently bound to either anti-mouse-Ig or anti-rabbit-Ig directed against the species-specific Fc part of the primary antibody.In brief, magnetic beads (MB) (Invitrogen, Oslo, Norway) were covalently bound to either anti-mouse-Ig or anti-rabbit-Ig directed against the species-specific Fc part of the primary antibody.. The following primary antibodies were used: FE65 (05–759, Millipore), GFP (sc 9996, Santa Cruz Biotechnology Darmstadt, Germany), SV2A (HPA007863, Sigma), and SERCA2 (NB-100–237, Novus Biologicals).. Cell extracts (500 μg of total protein) and 100 μl of MB were incubated together with a primary antibody (0.7 μg of antibody/50 μl of MB) for 48 h at 4 °C.Cell extracts (500 μg of total protein) and 100 μl of MB were incubated together with a primary antibody (0.7 μg of antibody/50 μl of MB) for 48 h at 4 °C.

    Article Title: Amyloid Beta A4 Precursor Protein-binding Family B Member 1 (FE65) Interactomics Revealed Synaptic Vesicle Glycoprotein 2A (SV2A) and Sarcoplasmic/Endoplasmic Reticulum Calcium ATPase 2 (SERCA2) as New Binding Proteins in the Human Brain
    Article Snippet: In brief, magnetic beads (MB) (Invitrogen, Oslo, Norway) were covalently bound to either anti-mouse-Ig or anti-rabbit-Ig directed against the species-specific Fc part of the primary antibody.In brief, magnetic beads (MB) (Invitrogen, Oslo, Norway) were covalently bound to either anti-mouse-Ig or anti-rabbit-Ig directed against the species-specific Fc part of the primary antibody.. The following primary antibodies were used: FE65 (05–759, Millipore), GFP (sc 9996, Santa Cruz Biotechnology Darmstadt, Germany), SV2A (HPA007863, Sigma), and SERCA2 (NB-100–237, Novus Biologicals).. Cell extracts (500 g of total protein) and 100 l of MB were incubated together with a primary antibody (0.7 g of antibody/50 l of MB) for 48 h at 4 °C.Cell extracts (500 g of total protein) and 100 l of MB were incubated together with a primary antibody (0.7 g of antibody/50 l of MB) for 48 h at 4 °C.

    Labeling:

    Article Title: Ca 2+ tunneling architecture and function are important for secretion
    Article Snippet: Primary antibody incubation was performed at 4°C overnight.Primary antibody incubation was performed at 4°C overnight.. The following primary antibodies were used at a 1:500 dilution: SERCA2 (NB300-581; Novus Biologicals), STIM1 (5668S; Cell Signaling and MAI-19451; Thermo Fisher Scientific), KRAP (14157-1-AP; Thermo Fisher Scientific), NFAT1 (5861S; Cell Signaling), and ANO1 (14476; Cell Signaling).. For IP 3 R1 detection, a custom-made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used ( ).For IP 3 R1 detection, a custom-made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used ( ).

    Article Title: Ca2+ tunneling architecture and function are important for secretion.
    Article Snippet: Primary antibody incubation was performed at 4°C overnight.Primary antibody incubation was performed at 4°C overnight.. The following primary antibodies were used at a 1:500 dilution: SERCA2 (NB300-581; Novus Biologicals), STIM1 (5668S; Cell Signaling and MAI-19451; Thermo Fisher Scientific), KRAP (14157-1-AP; Thermo Fisher Scientific), NFAT1 (5861S; Cell Signaling), and ANO1 (14476; Cell Signaling).. For IP3R1 detection, a custom-made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used (Baker et al., 2021).For IP3R1 detection, a custom-made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used (Baker et al., 2021).

    Article Title: Architecture of Ca 2+ tunneling, a basic Ca 2+ signaling modality important for secretion
    Article Snippet: Primary antibody incubation was performed at 4°C overnight.Primary antibody incubation was performed at 4°C overnight.. The following primary antibodies were used at a 1:500 dilution: SERCA2 (NB300-581, Novus Biologicals), STIM1 (5668S, Cell Signaling and MAI-19451, Fisher), KRAP (14157-1-AP, Fisher), NFAT1 (5861S, Cell Signaling), ANO1 (14476, Cell Signaling).. For IP 3 R1 detection a custom made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used .For IP 3 R1 detection a custom made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used .

    Article Title: Amyloid Beta A4 Precursor Protein-binding Family B Member 1 (FE65) Interactomics Revealed Synaptic Vesicle Glycoprotein 2A (SV2A) and Sarcoplasmic/Endoplasmic Reticulum Calcium ATPase 2 (SERCA2) as New Binding Proteins in the Human Brain
    Article Snippet: In brief, magnetic beads (MB) (Invitrogen, Oslo, Norway) were covalently bound to either anti-mouse-Ig or anti-rabbit-Ig directed against the species-specific Fc part of the primary antibody.In brief, magnetic beads (MB) (Invitrogen, Oslo, Norway) were covalently bound to either anti-mouse-Ig or anti-rabbit-Ig directed against the species-specific Fc part of the primary antibody.. The following primary antibodies were used: FE65 (05–759, Millipore), GFP (sc 9996, Santa Cruz Biotechnology Darmstadt, Germany), SV2A (HPA007863, Sigma), and SERCA2 (NB-100–237, Novus Biologicals).. Cell extracts (500 μg of total protein) and 100 μl of MB were incubated together with a primary antibody (0.7 μg of antibody/50 μl of MB) for 48 h at 4 °C.Cell extracts (500 μg of total protein) and 100 μl of MB were incubated together with a primary antibody (0.7 μg of antibody/50 μl of MB) for 48 h at 4 °C.

    Article Title: Amyloid Beta A4 Precursor Protein-binding Family B Member 1 (FE65) Interactomics Revealed Synaptic Vesicle Glycoprotein 2A (SV2A) and Sarcoplasmic/Endoplasmic Reticulum Calcium ATPase 2 (SERCA2) as New Binding Proteins in the Human Brain
    Article Snippet: In brief, magnetic beads (MB) (Invitrogen, Oslo, Norway) were covalently bound to either anti-mouse-Ig or anti-rabbit-Ig directed against the species-specific Fc part of the primary antibody.In brief, magnetic beads (MB) (Invitrogen, Oslo, Norway) were covalently bound to either anti-mouse-Ig or anti-rabbit-Ig directed against the species-specific Fc part of the primary antibody.. The following primary antibodies were used: FE65 (05–759, Millipore), GFP (sc 9996, Santa Cruz Biotechnology Darmstadt, Germany), SV2A (HPA007863, Sigma), and SERCA2 (NB-100–237, Novus Biologicals).. Cell extracts (500 g of total protein) and 100 l of MB were incubated together with a primary antibody (0.7 g of antibody/50 l of MB) for 48 h at 4 °C.Cell extracts (500 g of total protein) and 100 l of MB were incubated together with a primary antibody (0.7 g of antibody/50 l of MB) for 48 h at 4 °C.

    Expressing:

    Article Title: Ca 2+ tunneling architecture and function are important for secretion
    Article Snippet: Primary antibody incubation was performed at 4°C overnight.Primary antibody incubation was performed at 4°C overnight.. The following primary antibodies were used at a 1:500 dilution: SERCA2 (NB300-581; Novus Biologicals), STIM1 (5668S; Cell Signaling and MAI-19451; Thermo Fisher Scientific), KRAP (14157-1-AP; Thermo Fisher Scientific), NFAT1 (5861S; Cell Signaling), and ANO1 (14476; Cell Signaling).. For IP 3 R1 detection, a custom-made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used ( ).For IP 3 R1 detection, a custom-made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used ( ).

    Article Title: Ca2+ tunneling architecture and function are important for secretion.
    Article Snippet: Primary antibody incubation was performed at 4°C overnight.Primary antibody incubation was performed at 4°C overnight.. The following primary antibodies were used at a 1:500 dilution: SERCA2 (NB300-581; Novus Biologicals), STIM1 (5668S; Cell Signaling and MAI-19451; Thermo Fisher Scientific), KRAP (14157-1-AP; Thermo Fisher Scientific), NFAT1 (5861S; Cell Signaling), and ANO1 (14476; Cell Signaling).. For IP3R1 detection, a custom-made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used (Baker et al., 2021).For IP3R1 detection, a custom-made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used (Baker et al., 2021).

    Article Title: Architecture of Ca 2+ tunneling, a basic Ca 2+ signaling modality important for secretion
    Article Snippet: Primary antibody incubation was performed at 4°C overnight.Primary antibody incubation was performed at 4°C overnight.. The following primary antibodies were used at a 1:500 dilution: SERCA2 (NB300-581, Novus Biologicals), STIM1 (5668S, Cell Signaling and MAI-19451, Fisher), KRAP (14157-1-AP, Fisher), NFAT1 (5861S, Cell Signaling), ANO1 (14476, Cell Signaling).. For IP 3 R1 detection a custom made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used .For IP 3 R1 detection a custom made monoclonal antibody targeting the following peptide: RIGLLGHPPHMNVNPQQPA (ProSci) was used .

    Article Title: Amyloid Beta A4 Precursor Protein-binding Family B Member 1 (FE65) Interactomics Revealed Synaptic Vesicle Glycoprotein 2A (SV2A) and Sarcoplasmic/Endoplasmic Reticulum Calcium ATPase 2 (SERCA2) as New Binding Proteins in the Human Brain
    Article Snippet: In brief, magnetic beads (MB) (Invitrogen, Oslo, Norway) were covalently bound to either anti-mouse-Ig or anti-rabbit-Ig directed against the species-specific Fc part of the primary antibody.In brief, magnetic beads (MB) (Invitrogen, Oslo, Norway) were covalently bound to either anti-mouse-Ig or anti-rabbit-Ig directed against the species-specific Fc part of the primary antibody.. The following primary antibodies were used: FE65 (05–759, Millipore), GFP (sc 9996, Santa Cruz Biotechnology Darmstadt, Germany), SV2A (HPA007863, Sigma), and SERCA2 (NB-100–237, Novus Biologicals).. Cell extracts (500 μg of total protein) and 100 μl of MB were incubated together with a primary antibody (0.7 μg of antibody/50 μl of MB) for 48 h at 4 °C.Cell extracts (500 μg of total protein) and 100 μl of MB were incubated together with a primary antibody (0.7 μg of antibody/50 μl of MB) for 48 h at 4 °C.

    Article Title: Amyloid Beta A4 Precursor Protein-binding Family B Member 1 (FE65) Interactomics Revealed Synaptic Vesicle Glycoprotein 2A (SV2A) and Sarcoplasmic/Endoplasmic Reticulum Calcium ATPase 2 (SERCA2) as New Binding Proteins in the Human Brain
    Article Snippet: In brief, magnetic beads (MB) (Invitrogen, Oslo, Norway) were covalently bound to either anti-mouse-Ig or anti-rabbit-Ig directed against the species-specific Fc part of the primary antibody.In brief, magnetic beads (MB) (Invitrogen, Oslo, Norway) were covalently bound to either anti-mouse-Ig or anti-rabbit-Ig directed against the species-specific Fc part of the primary antibody.. The following primary antibodies were used: FE65 (05–759, Millipore), GFP (sc 9996, Santa Cruz Biotechnology Darmstadt, Germany), SV2A (HPA007863, Sigma), and SERCA2 (NB-100–237, Novus Biologicals).. Cell extracts (500 g of total protein) and 100 l of MB were incubated together with a primary antibody (0.7 g of antibody/50 l of MB) for 48 h at 4 °C.Cell extracts (500 g of total protein) and 100 l of MB were incubated together with a primary antibody (0.7 g of antibody/50 l of MB) for 48 h at 4 °C.



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