Journal: iScience

Article Title: Taurine ameliorates blood pressure and vascular function in patients with type 2 diabetes: Randomized, double-blind, placebo-controlled trial

doi: 10.1016/j.isci.2025.112719

Figure Lengend Snippet: Effects of high glucose conditions on TRPC6, SERCA2, SERCA3, STIM1, and CaMKII (A and B) The protein expression levels of TRPC6, SERCA2, SERCA3, STIM1, and CaMKII in the presence of high glucose (15, 25, and 35 mmol/L) plus thapsigargin (TG 1 μmol/L) in a dose-dependent (A) and time-dependent (B) manner in platelets from healthy control individuals. The data are presented as the means ± SDs. ∗ p < 0.05,∗∗ p < 0.01, and ∗∗∗ p < 0.001, vs. the control (Cont) without high glucose treatment.

Article Snippet: The primary antibodies used were, including TRPC6 (Alomone, Israel), SERCA2, SERCA3 (Santa Cruz, USA), STIM1 (Abcam), CaMKII (Abcam), and followed by incubated with the secondary antibodies (ZSGB-BIO).

Techniques: Expressing, Control

Journal: iScience

Article Title: Taurine ameliorates blood pressure and vascular function in patients with type 2 diabetes: Randomized, double-blind, placebo-controlled trial

doi: 10.1016/j.isci.2025.112719

Figure Lengend Snippet: Effects of high glucose and taurine on TRPC6, SERCA2, SERCA3, STIM1, and CaMKII (A and B) The protein expression levels of TRPC6, SERCA2, SERCA3, STIM1, and CaMKII in platelets from healthy control individuals treated with high glucose (25 mmol/L) or high glucose plus NaHS (40 mmol/L) were detected via western blotting (A). The data were quantified from bands from three independent immunoblots, and the relative expression levels are shown (B). The data are presented as the means ± SDs. ∗ p < 0.05,∗∗ p < 0.01, vs. high glucose treatment.

Article Snippet: The primary antibodies used were, including TRPC6 (Alomone, Israel), SERCA2, SERCA3 (Santa Cruz, USA), STIM1 (Abcam), CaMKII (Abcam), and followed by incubated with the secondary antibodies (ZSGB-BIO).

Techniques: Expressing, Control, Western Blot

Journal: iScience

Article Title: Taurine ameliorates blood pressure and vascular function in patients with type 2 diabetes: Randomized, double-blind, placebo-controlled trial

doi: 10.1016/j.isci.2025.112719

Figure Lengend Snippet: SERCA2, TRPC6, SERCA3, STIM1, and CaMKII proteins in patients with T2D and healthy control individuals (NT) (A–E) The protein expression levels of STIM1 (A), TRPC6 (B), CaMKII (C), SERCA2 (D),and SERCA3 (E) in patients with T2D and healthy control individuals (NT). (F) The data were quantified from bands from three independent immunoblots, and the relative expression levels are shown. The data are presented as the means ± SDs. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001, compared with the NT group. The data were quantified from bands from three independent immunoblots, and the relative expression levels are shown.

Article Snippet: The primary antibodies used were, including TRPC6 (Alomone, Israel), SERCA2, SERCA3 (Santa Cruz, USA), STIM1 (Abcam), CaMKII (Abcam), and followed by incubated with the secondary antibodies (ZSGB-BIO).

Techniques: Control, Expressing, Western Blot

Journal: The Journal of investigative dermatology

Article Title: Impaired trafficking of the desmoplakins in cultured Darier's disease keratinocytes.

doi: 10.1046/j.1523-1747.2003.12557.x

Figure Lengend Snippet: Figure 6. Dp associates with SERCA2 during di¡erentiation. (A) NHK cells grown in low-calcium medium were pulse-labeled with [35S]methionine and cysteine and then chased in a normal-calcium med- ium for 4 h. Cells were lyzed and immunoprecipitated with the indicated antibody. (B) NHK cells were incubated in normal-calcium medium for 4 h to induce cell di¡erentiation. Cells were lyzed and immunoprecipitated with anti-SERCA2 antibody. SERCA2 immunoprecipitates were analyzed by SDS-PAGE and western blot using an anti-Dp antibody (lane 2). Lane 1, Western blot of total cell extract.

Article Snippet: Mouse monoclonal anti-PG antibody was purchased from Cymbus Bioscience (Southampton, UK), goat polyclonal anti-SERCA2 antibody was from Santa Cruz Biotechnology (Insight Biotechnology, Middlesex, UK), and rabbit polyclonal anti-calnexin antibody was obtained from Calbiochem (CN Biosciences, Nottingham, UK).

Techniques: Labeling, Immunoprecipitation, Incubation, SDS Page, Western Blot

Journal: PLoS ONE

Article Title: Low Density Lipoproteins Promote Unstable Calcium Handling Accompanied by Reduced SERCA2 and Connexin-40 Expression in Cardiomyocytes

doi: 10.1371/journal.pone.0058128

Figure Lengend Snippet: Real-time PCR analysis showing SERCA2 (A), RyR2 (B), IP3-RI (C) and IP3-RIII mRNA expression levels in HL-1 cardiomyocytes exposed to increasing doses of LDL. Data were processed with a specially designed software programme based on Ct value of each sample and normalized to ARBP mRNA. Values significantly different from the level with 0 µg/ml LDL are indicated with asterisks.

Article Snippet: Blots were incubated with monoclonal antibodies against SERCA2 (Novus Biologicals NB100–237), Cx40 (Invitrogen, 36–4900) and Cx43 (Sigma-Aldrich C6219).

Techniques: Real-time Polymerase Chain Reaction, Expressing, Software

Journal: PLoS ONE

Article Title: Low Density Lipoproteins Promote Unstable Calcium Handling Accompanied by Reduced SERCA2 and Connexin-40 Expression in Cardiomyocytes

doi: 10.1371/journal.pone.0058128

Figure Lengend Snippet: Representative Western blot analysis showing SERCA2 bands in HL-1 cells exposed to increasing LDL concentrations (0, 50, 100 and 200 µg/mL) ( A ) or to similar dose (500 µg/ml) of LDL or HDL ( B ) for 24 hours. The bar graph below shows SERCA2 band quantification. Unchanged levels of β-tubulin are shown as loading control. Results are mean±SEM of three independent experiments performed in duplicate. *P<0.05 versus. HL-1 cells incubated in absence of LDL. C Calcium images acquired at rest before (CON) and during a rapid caffeine application. D Representative calcium transients recorded in cultures incubated without lipoproteins (CON), with 500 µg/ml LDL or with 500 µg/ml HDL. The lower panel shows the corresponding time integrals of the calcium transients. E summary of the effect of LDL and HDL on the time integral of the caffeine induced calcium transient. *P<0.05 vs. cells incubated with LDL

Article Snippet: Blots were incubated with monoclonal antibodies against SERCA2 (Novus Biologicals NB100–237), Cx40 (Invitrogen, 36–4900) and Cx43 (Sigma-Aldrich C6219).

Techniques: Western Blot, Control, Incubation