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Pathological examination. All images were obtained using cluster of differentiation <t>34/periodic</t> <t>acid-Schiff</t> immunohistochemical and histochemical double staining. VM is indicated by red arrows and normal blood vessels are indicated by black arrows. (A) Single VM channel with an adjacent normal blood vessel (magnification, ×120). (B) Representative VM structure and adjacent endothelial-lined vessel (magnification, ×110). (C) Multiple VM channels with normal blood vessels in the same field. (D) Clustered VM channels in tumor tissue (magnification, ×75; scale bar, 50 µm). VM, vasculogenic mimicry.
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Pathological examination. All images were obtained using cluster of differentiation <t>34/periodic</t> <t>acid-Schiff</t> immunohistochemical and histochemical double staining. VM is indicated by red arrows and normal blood vessels are indicated by black arrows. (A) Single VM channel with an adjacent normal blood vessel (magnification, ×120). (B) Representative VM structure and adjacent endothelial-lined vessel (magnification, ×110). (C) Multiple VM channels with normal blood vessels in the same field. (D) Clustered VM channels in tumor tissue (magnification, ×75; scale bar, 50 µm). VM, vasculogenic mimicry.
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Pathological examination. All images were obtained using cluster of differentiation <t>34/periodic</t> <t>acid-Schiff</t> immunohistochemical and histochemical double staining. VM is indicated by red arrows and normal blood vessels are indicated by black arrows. (A) Single VM channel with an adjacent normal blood vessel (magnification, ×120). (B) Representative VM structure and adjacent endothelial-lined vessel (magnification, ×110). (C) Multiple VM channels with normal blood vessels in the same field. (D) Clustered VM channels in tumor tissue (magnification, ×75; scale bar, 50 µm). VM, vasculogenic mimicry.
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periodic acid schiff pas reagent  (Servicebio Inc)
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Pathological examination. All images were obtained using cluster of differentiation <t>34/periodic</t> <t>acid-Schiff</t> immunohistochemical and histochemical double staining. VM is indicated by red arrows and normal blood vessels are indicated by black arrows. (A) Single VM channel with an adjacent normal blood vessel (magnification, ×120). (B) Representative VM structure and adjacent endothelial-lined vessel (magnification, ×110). (C) Multiple VM channels with normal blood vessels in the same field. (D) Clustered VM channels in tumor tissue (magnification, ×75; scale bar, 50 µm). VM, vasculogenic mimicry.
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Pathological examination. All images were obtained using cluster of differentiation 34/periodic acid-Schiff immunohistochemical and histochemical double staining. VM is indicated by red arrows and normal blood vessels are indicated by black arrows. (A) Single VM channel with an adjacent normal blood vessel (magnification, ×120). (B) Representative VM structure and adjacent endothelial-lined vessel (magnification, ×110). (C) Multiple VM channels with normal blood vessels in the same field. (D) Clustered VM channels in tumor tissue (magnification, ×75; scale bar, 50 µm). VM, vasculogenic mimicry.

Journal: Oncology Letters

Article Title: Vasculogenic mimicry and breast cancer prognosis: Insights into predictive model development

doi: 10.3892/ol.2026.15600

Figure Lengend Snippet: Pathological examination. All images were obtained using cluster of differentiation 34/periodic acid-Schiff immunohistochemical and histochemical double staining. VM is indicated by red arrows and normal blood vessels are indicated by black arrows. (A) Single VM channel with an adjacent normal blood vessel (magnification, ×120). (B) Representative VM structure and adjacent endothelial-lined vessel (magnification, ×110). (C) Multiple VM channels with normal blood vessels in the same field. (D) Clustered VM channels in tumor tissue (magnification, ×75; scale bar, 50 µm). VM, vasculogenic mimicry.

Article Snippet: Periodic acid-Schiff staining kit , Beyotime Institute of Biotechnology , C0142 , Ready-to-use.

Techniques: Immunohistochemical staining, Double Staining