sb203580 (Tocris)
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sb203580
Sb203580, supplied by Tocris, used in various techniques. Bioz Stars score: 96/100, based on 1024 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sb203580/SB+203580/pm42854645-45-2-9
Average 96 stars, based on 1024 article reviews
Sb203580, supplied by Tocris, used in various techniques. Bioz Stars score: 96/100, based on 1024 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sb203580/SB+203580/pm42854645-45-2-9
Average 96 stars, based on 1024 article reviews
sb203580 - by Bioz Stars,
2026-11
96/100 stars
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Knock-Out:Article Title: Isolated naive pluripotent stem cells and methods of generating same Article Snippet: Instead of KO-DMEM the following media can also be alternatively used: DMEM-F12 (Biological Industries or Invitrogen), KO-DMEM/F12 (Invitrogenl2660-012), GMEM (Invitrogen 11710) or 1:1 DMEM/F12:Neurobasal mix (Invitrogen 21103-049). .. Following is a non-limiting example of an alternative WIS-NHSM medium (termed “WIS-NHSM-ii”) which was used to culture the naive cells of some embodiments of the invention: 425 ml Knockout DMEM (Invitrogen 10829), 74 ml of knockout serum replacement (Invitrogen 10828-028), 5 ml N2 supplement (Invitrogen 17502048), 10 μg of recombinant human LIF (Millipore, LIF1005), 8 ng/ml recombinant bFGF and 1 ng/ml recombinant TGFP 1 (Peprotech), 1 mM glutamine (Invitrogen), 1% nonessential amino acids (Invitrogen), 0.1 mM 0-mercaptoethanol (Sigma), Penicillin-Streptomycin (Invitrogen) and small molecule inhibitors: PD0325901 (1 μM, ERK1/2i, AXONMEDCHEM); CHIR99021 (3 μM, GSK3bi, AXON MEDCHEM); Article Title: Isolated naive pluripotent stem cells and methods of generating same Article Snippet: .. WIS-NHSM-(i) medium was generated by including: 475 ml Knockout DMEM (Invitrogen 10829), 5 grams AlbuMAX (Invitrogen 11020-021), 5 ml N2 supplement (Invitrogen 17502048), 10 μg (micrograms) of recombinant human LIF (leukemia inhibitory factor) (Peprotech), 8 ng/ml recombinant bFGF (Peprotech) and 1 ng/ml recombinant TGFP 1 (Peprotech), 1 mM glutamine (Invitrogen), 1% nonessential amino acids (Invitrogen), 0.1 mM β-mercaptoethanol (Sigma), Penicillin-Streptomycin (Invitrogen) and small molecule inhibitors: PD0325901 (1 μM, ERK1/2i, Axon Medchem); CHIR99021 (3 μM, GSK3βi, Axon Medchem); SP600125 (10 μM, JNKi, TOCRIS), Article Title: Isolated naive pluripotent stem cells and methods of generating same Article Snippet: Following is a non-limiting example of an alternative WIS-NHSM medium (termed “WIS-NHSM-ii”) which was used to culture the naive cells of some embodiments of the invention: 425 ml Knockout DMEM (Invitrogen 10829), 74 ml of knockout serum replacement (Invitrogen 10828-028), 5 ml N2 supplement (Invitrogen 17502048), 10 μg of recombinant human LIF (Millipore, LIF1005), 8 ng/ml recombinant bFGF and 1 ng/ml recombinant TGFP 1 (Peprotech), 1 mM glutamine (Invitrogen), 1% nonessential amino acids (Invitrogen), 0.1 mM 0-mercaptoethanol (Sigma), Penicillin-Streptomycin (Invitrogen) and small molecule inhibitors: PD0325901 (1 μM, ERK1/2i, AXONMEDCHEM); CHIR99021 (3 μM, GSK3bi, AXON MEDCHEM); SB203580 (5 μM, p38i, TOCRIS); SP600125 (5 μM, JNKi, TOCRIS) with or without Y-27632 (5 μM, ROCKi Axon Medchem). .. Alternative composition of WIS-NHSM medium involved replacing 15% KSR with 1% albumax: 500 ml Knockout DMEM (Invitrogen 10829), 5 gr of AlbuMAX (Invitrogen 11020-021), 5 ml N2 supplement (Invitrogen 17502048), 10 μg of recombinant human LIF (Millipore, LIF1005), 8 ng/ml recombinant bFGF and 1 ng/ml recombinant TGFβ31 (Peprotech), 1 mM glutamine (Invitrogen), 1% nonessential amino acids (Invitrogen), 0.1 mM β-mercaptoethanol (Sigma), Penicillin-Streptomycin (Invitrogen) and small molecule inhibitors: PD0325901 (1 μM, ERK1/2i, AXONMEDCHEM); CHIR99021 (3 μM, GSK3bi, AXON MEDCHEM); Recombinant:Article Title: Isolated naive pluripotent stem cells and methods of generating same Article Snippet: Instead of KO-DMEM the following media can also be alternatively used: DMEM-F12 (Biological Industries or Invitrogen), KO-DMEM/F12 (Invitrogenl2660-012), GMEM (Invitrogen 11710) or 1:1 DMEM/F12:Neurobasal mix (Invitrogen 21103-049). .. Following is a non-limiting example of an alternative WIS-NHSM medium (termed “WIS-NHSM-ii”) which was used to culture the naive cells of some embodiments of the invention: 425 ml Knockout DMEM (Invitrogen 10829), 74 ml of knockout serum replacement (Invitrogen 10828-028), 5 ml N2 supplement (Invitrogen 17502048), 10 μg of recombinant human LIF (Millipore, LIF1005), 8 ng/ml recombinant bFGF and 1 ng/ml recombinant TGFP 1 (Peprotech), 1 mM glutamine (Invitrogen), 1% nonessential amino acids (Invitrogen), 0.1 mM 0-mercaptoethanol (Sigma), Penicillin-Streptomycin (Invitrogen) and small molecule inhibitors: PD0325901 (1 μM, ERK1/2i, AXONMEDCHEM); CHIR99021 (3 μM, GSK3bi, AXON MEDCHEM); Article Title: Isolated naive pluripotent stem cells and methods of generating same Article Snippet: .. WIS-NHSM-(i) medium was generated by including: 475 ml Knockout DMEM (Invitrogen 10829), 5 grams AlbuMAX (Invitrogen 11020-021), 5 ml N2 supplement (Invitrogen 17502048), 10 μg (micrograms) of recombinant human LIF (leukemia inhibitory factor) (Peprotech), 8 ng/ml recombinant bFGF (Peprotech) and 1 ng/ml recombinant TGFP 1 (Peprotech), 1 mM glutamine (Invitrogen), 1% nonessential amino acids (Invitrogen), 0.1 mM β-mercaptoethanol (Sigma), Penicillin-Streptomycin (Invitrogen) and small molecule inhibitors: PD0325901 (1 μM, ERK1/2i, Axon Medchem); CHIR99021 (3 μM, GSK3βi, Axon Medchem); SP600125 (10 μM, JNKi, TOCRIS), Article Title: Isolated naive pluripotent stem cells and methods of generating same Article Snippet: Following is a non-limiting example of an alternative WIS-NHSM medium (termed “WIS-NHSM-ii”) which was used to culture the naive cells of some embodiments of the invention: 425 ml Knockout DMEM (Invitrogen 10829), 74 ml of knockout serum replacement (Invitrogen 10828-028), 5 ml N2 supplement (Invitrogen 17502048), 10 μg of recombinant human LIF (Millipore, LIF1005), 8 ng/ml recombinant bFGF and 1 ng/ml recombinant TGFP 1 (Peprotech), 1 mM glutamine (Invitrogen), 1% nonessential amino acids (Invitrogen), 0.1 mM 0-mercaptoethanol (Sigma), Penicillin-Streptomycin (Invitrogen) and small molecule inhibitors: PD0325901 (1 μM, ERK1/2i, AXONMEDCHEM); CHIR99021 (3 μM, GSK3bi, AXON MEDCHEM); SB203580 (5 μM, p38i, TOCRIS); SP600125 (5 μM, JNKi, TOCRIS) with or without Y-27632 (5 μM, ROCKi Axon Medchem). .. Alternative composition of WIS-NHSM medium involved replacing 15% KSR with 1% albumax: 500 ml Knockout DMEM (Invitrogen 10829), 5 gr of AlbuMAX (Invitrogen 11020-021), 5 ml N2 supplement (Invitrogen 17502048), 10 μg of recombinant human LIF (Millipore, LIF1005), 8 ng/ml recombinant bFGF and 1 ng/ml recombinant TGFβ31 (Peprotech), 1 mM glutamine (Invitrogen), 1% nonessential amino acids (Invitrogen), 0.1 mM β-mercaptoethanol (Sigma), Penicillin-Streptomycin (Invitrogen) and small molecule inhibitors: PD0325901 (1 μM, ERK1/2i, AXONMEDCHEM); CHIR99021 (3 μM, GSK3bi, AXON MEDCHEM); Article Title: Isolated naive pluripotent stem cells and methods of generating same Article Snippet: .. Small molecule compounds and cytokines—Small molecules and cytokines were purchased from Tocris, Calbiochem, Stemgent, Peprotech or Sigma, and were supplemented as indicated at the following final concentration: JAK inhibitor (420099 JAKi, 0.6 M—CAL BIOCHEM), Kenopaullone (KP, 5 μM, Sigma Aldrich), PD0325901 (PD, 1 μM, Axon Medchem); CHIR99021 (CH, 3 μM, AXON MEDCHEM), Forskolin (FK, 10 μM, TOCRIS), FGF4-Receptor inhibitors PD173074 (0.1 μM, TOCRIS) and SU5401 (2 μM, TOCRIS); TGFβ/ALK inhibitors A83-01 (1 μM, STEMGENT), PKC inhibitor Go6983 (1 μM,TOCRIS), ALK inhibitor (ALKi: SB431542, 2 μM,TOCRIS); AICAR (0.5 mM); Bix01294 (1 μM); BayK8644 (1 μM, Stemgent); Generated:Article Title: Isolated naive pluripotent stem cells and methods of generating same Article Snippet: .. WIS-NHSM-(i) medium was generated by including: 475 ml Knockout DMEM (Invitrogen 10829), 5 grams AlbuMAX (Invitrogen 11020-021), 5 ml N2 supplement (Invitrogen 17502048), 10 μg (micrograms) of recombinant human LIF (leukemia inhibitory factor) (Peprotech), 8 ng/ml recombinant bFGF (Peprotech) and 1 ng/ml recombinant TGFP 1 (Peprotech), 1 mM glutamine (Invitrogen), 1% nonessential amino acids (Invitrogen), 0.1 mM β-mercaptoethanol (Sigma), Penicillin-Streptomycin (Invitrogen) and small molecule inhibitors: PD0325901 (1 μM, ERK1/2i, Axon Medchem); CHIR99021 (3 μM, GSK3βi, Axon Medchem); SP600125 (10 μM, JNKi, TOCRIS), Incubation:Article Title: Hexavalent Chromium Induces Apoptosis and Autophagy in Human Neurons and Astrocytes via MAPK Pathway Activation. Article Snippet: .. U0126 (MEK1/2 inhibitor; Cell-signaling, USA), Concentration Assay:Article Title: Hexavalent Chromium Induces Apoptosis and Autophagy in Human Neurons and Astrocytes via MAPK Pathway Activation. Article Snippet: .. U0126 (MEK1/2 inhibitor; Cell-signaling, USA), Article Title: Isolated naive pluripotent stem cells and methods of generating same Article Snippet: .. Small molecule compounds and cytokines—Small molecules and cytokines were purchased from Tocris, Calbiochem, Stemgent, Peprotech or Sigma, and were supplemented as indicated at the following final concentration: JAK inhibitor (420099 JAKi, 0.6 M—CAL BIOCHEM), Kenopaullone (KP, 5 μM, Sigma Aldrich), PD0325901 (PD, 1 μM, Axon Medchem); CHIR99021 (CH, 3 μM, AXON MEDCHEM), Forskolin (FK, 10 μM, TOCRIS), FGF4-Receptor inhibitors PD173074 (0.1 μM, TOCRIS) and SU5401 (2 μM, TOCRIS); TGFβ/ALK inhibitors A83-01 (1 μM, STEMGENT), PKC inhibitor Go6983 (1 μM,TOCRIS), ALK inhibitor (ALKi: SB431542, 2 μM,TOCRIS); AICAR (0.5 mM); Bix01294 (1 μM); BayK8644 (1 μM, Stemgent); |
