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sag  (Tocris)


Bioz Verified Symbol Tocris is a verified supplier
Bioz Manufacturer Symbol Tocris manufactures this product  
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    Structured Review

    Tocris sag
    Sag, supplied by Tocris, used in various techniques. Bioz Stars score: 95/100, based on 73 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sag/SAG/bio_rxiv__64898__2026__04__22__719920-92-24-26
    Average 95 stars, based on 73 article reviews
    sag - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Recombinant:

    Article Title: Hedgehog signaling drives glial cell plasticity and oncogenic reprogramming in gastroenteropancreatic neuroendocrine neoplasms
    Article Snippet: .. Drug compounds used in the studies include: human recombinant SHH N-terminal peptide (R&D Systems, Cat# 1845-GMP), SAG (Tocris, Cat# 4366), vismodegib (Tocris, Cat# 7710), sonidegib (Tocris, Cat# 7826), GANT61 (Tocris, Cat# 3191), and itraconazole (Tocris, Cat# 5981). .. Men1 knockdown was accomplished using ON-TARGETplus Mouse Men1 siRNA (Horizon Discovery, Cat# L-042675-01-0005, Layfayette, CO).

    Article Title: Vascular-Perfusable Human 3D Brain-on-Chip
    Article Snippet: Media was composed of EGM-2 MV from Lonza (CC-3202) supplemented with 50 ng/mL VEGF-165 (human recombinant protein, PeproTech, 100-20) for one week, reduced to 10 ng/mL thereafter. .. BBB-Chip media additionally included 10 ng/mL of PDGF-BB (human recombinant protein, PeproTech, AF-100-14B) while NVU-Chip additionally included 1 nM of SAG (potent Smoothened receptor agonist, Tocris, 4366). .. Brain-Chip media was composed of EGM-2 MV with all supplements except FBS, in addition to VEGF-165 as described above, 1% v/v AGS (ScienCell, 1852), 2% v/v B-27 (Gibco, 17504044), 1 μM cAMP (N6,2′-O-Dibutyryladenosine 3′,5′-cyclic monophosphate sodium salt, Millipore Sigma, D0627), 10 ng/mL NT-3 (Peprotech, 450-03), 1 nM SAG (Tocris, 4366), and 25 ng/mL M-CSF (human recombinant protein, PeproTech, 300-25).

    Article Title: Hedgehog signaling drives glial cell plasticity and oncogenic reprogramming in gastroenteropancreatic neuroendocrine neoplasms.
    Article Snippet: .. Drug compounds used in the studies include: human recombinant SHH N-terminal peptide (R&D Systems, Cat# 1845-GMP), SAG (Tocris, Cat# 4366), vismodegib (Tocris, Cat# 7710), sonidegib (Tocris, Cat# 7826), GANT61 (Tocris, Cat# 3191), and itraconazole (Tocris, Cat# 5981). .. Drug compounds used in the studies include: human recombinant SHH N-terminal peptide (R&D Systems, Cat# 1845-GMP), SAG (Tocris, Cat# 4366), vismodegib (Tocris, Cat# 7710), sonidegib (Tocris, Cat# 7826), GANT61 (Tocris, Cat# 3191), and itraconazole (Tocris, Cat# 5981).

    other:

    Article Title: Hierarchical TBX6-FOXC Regulatory Logic Controls Human Trunk Mesoderm Diversification
    Article Snippet: The following day (Day 0), cells were incubated in Nutristem with 6μM CHIR (ABCR, AB253776) and 100 nM PIK90 (Tocris, 7902).

    Article Title: KMT2D-deficiency destabilizes lineage progression in immature neural progenitors
    Article Snippet: Day-7 NPCs were passaged using Dispase (StemCell Technologies, #07923) and replated at a density of 1-2 × 105 cells per well in a 24-well plate (Corning, #3524) with warmed OPC differentiation medium (DMEM/F12 (Gibco, #11965-092), 1% N2 supplement (Gibco, #17502048), 1% B27 supplement (Gibco, #17504044), bFGF (StemCell Technologies, #PHG0266, 20 ng/mL), SAG (Tocris Bioscience, #4366, 1 μM), and PDGF-AA (R&D Systems, #221-AA-010, 10 ng/mL).

    Cell Culture:

    Article Title: Engineered 3D immuno-glial-neurovascular human miBrain model
    Article Snippet: .. Validated iPSC-differentiated cells were co-cultured in miBrains at the following ratios: 5 M/mL BMECs, 0.2 M/mL pericytes, 1.5 M/mL astrocytes, 0.75 M/mL OPCs, 5 M/mL neurons, and 0.6 M/mL iMG, unless stated otherwise. miBrains were cultured in miBrain cell culture medium consisting of Human Endothelial Serum-Free Medium (ThermoFisher Scientific) with 1% pen/strep (Sigma-Aldrich), 1% NEAA (ThermoFisher Scientific), 1% CD lipids 20 (ThermoFisher Scientific), 1% Astrocyte Growth Supplement (ScienCell), 2% B-27 (ThermoFisher Scientific), 10 μL/ mL Insulin (ThermoFisher Scientific), 1 μM db-cAMP (Sigma Aldrich), 50 μg/ mL Ascorbic Acid (Sigma Aldrich), 10 ng/ mL NT-3 (PeproTech), 10 ng/ mL IGF-I (PeproTech), 100 ng/ mL Biotin (Sigma Aldrich), 60 ng/mL T3 (Sigma Aldrich), 4 μg/ mL Folic Acid (Sigma Aldrich) supplemented, 1 nM SAG (Tocris), and 50 ng/ mL VEGF-A (PeproTech). .. Upon the incorporation of iMG, 25 ng/mL M-CSF 25 (Sigma Aldrich) was added and maintained in the media and after week 2, VEGF-A concentration was reduced to 10 ng/mL.

    Article Title: A human iPSC-derived motor neuron-myogenic cell coculture platform to evaluate neuromuscular junction innervation after axon injury and in Spinal Muscular Atrophy
    Article Snippet: hiPSCs were differentiated to motor neurons for 14 days in N2B27 medium (1⁄2 DMEM/F12 (ThermoFisher, #11320082) and 1⁄2 Neurobasal-A media (Life technologies, #10888-022) supplemented with 1x N2 (Gibco, #17-502-048), 1x B27 (Gibco, #17-504-044), 1x GlutaMAX and 100□μM non-essential amino-acids. .. During the first 7 days, cells were cultured in N2B27 medium containing 10 μM SB431542 (Cayman Chemical, #13031), 100 nM LDN-193189 (Sigma, #SML0550), 1 μM Retinoic acid (Sigma, #R2625), 1 μM SAG (Tocris, #4366). .. For the subsequent 7 days, cells were maintained in N2B27 medium with 1 μM Retinoic acid, 1 μM SAG, 4 μM SU5402 (Sigma, #SML0443), and 5 μM DAPT (Tocris, #2634).



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