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Image Search Results
Journal: bioRxiv
Article Title: A Hotspot Phosphorylation Site on SHP2 Drives Oncoprotein Activation and Drug Resistance
doi: 10.1101/2025.06.11.659120
Figure Lengend Snippet: (A) Kinase Library prediction heatmap based on the peptide primary sequence of the SHP2 Y62 phosphosite plotted on human kinome evolutionary tree. ( B) SFK multi-kinase and selective inhibitors with targets shown, tested here. ( C-D ) Immunoblot analysis of ( C ) U-2 OS cells and ( D ) HCC827 cells treated with dasatinib and SU6656 at indicated concentrations, and DMSO. ( E-F ) Immunoblot analysis of HCC827 cells treated with ( E ) saracatinib, CH6953755, and PP2, and ( F ) double and triple combinations at indicated concentrations, and DMSO. ( G ) Immunoblot analysis of SYF knock out and wildtype MEFs. ( H ) In vitro kinase assays with recombinant SRC, YES1, and FYN kinases and recombinant SHP2 WT and Y62F. The SHP2 pY542 antibody demonstrated nonspecific binding in the control conditions.
Article Snippet:
Techniques: Sequencing, Phospho-proteomics, Western Blot, Knock-Out, In Vitro, Recombinant, Binding Assay, Control