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thermal cycling  (Bio-Rad)


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    Structured Review

    Bio-Rad thermal cycling
    Thermal Cycling, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/s1000/S1000+Firmware+Update+for+Serial+Numbers+SC010000+or+Higher/pmc12994450-216-0-2
    Average 99 stars, based on 28 article reviews
    thermal cycling - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    other:

    Article Title: Physical crowding in 3D niche regulates neural stem cell differentiation via Cx43-mediated gap junction communication
    Article Snippet: S1000 Touch Thermal Cycler , Bio Rad , S1000.

    Article Title: Effects of grassland degradation on diversity of arbuscular mycorrhizal fungi of a pioneer plant.
    Article Snippet: Arbuscular mycorrhizal fungi (AMF) are obligate symbionts that engage in crucial interactions with plants, playing a vital role in grassland ecology.. Our study focuses on the pioneer plant Agropyron cristatum, and we collected soil samples from four degraded grasslands in Yudaokou to investigate the response of community composition to the succession of degraded grasslands.. We measured the vegetation status, soil physical and chemical properties, AMF colonization, and spore density in different degraded grasslands.

    cDNA Synthesis:

    Article Title: Substrate Stiffness Regulates the Osteogenesis of PDLSCs Via ERK-Mediated YAP Nuclear Translocation
    Article Snippet: .. Complementary DNA (cDNA) synthesis was then synthesized with the PrimeScript RT kit (Takara, Tokyo, Japan) and a reverse transcription instrument, the S1000 (Bio-Rad, Hercules, CA). .. The transcriptional levels of the genes Runx2 and OCN were determined using the SYBR Premix Ex Taq II kit (Takara) on a 7500 sequence detection system (Applied Biosystems, Foster City, CA), according to the manufacturer's protocol.

    Article Title: Substrate Stiffness Regulates the Osteogenesis of PDLSCs Via ERK-Mediated YAP Nuclear Translocation.
    Article Snippet: .. Complementary DNA (cDNA) synthesis was then synthesized with the PrimeScript RT kit (Takara, Tokyo, Japan) and a reverse transcription instrument, the S1000 (Bio-Rad, Hercules, CA). .. The transcriptional levels of the genes Runx2 and OCN were determined using the SYBR Premix Ex Taq II kit (Takara) on a 7500 sequence detection system (Applied Biosystems, Foster City, CA), according to the manufacturer’s protocol.

    Synthesized:

    Article Title: Substrate Stiffness Regulates the Osteogenesis of PDLSCs Via ERK-Mediated YAP Nuclear Translocation
    Article Snippet: .. Complementary DNA (cDNA) synthesis was then synthesized with the PrimeScript RT kit (Takara, Tokyo, Japan) and a reverse transcription instrument, the S1000 (Bio-Rad, Hercules, CA). .. The transcriptional levels of the genes Runx2 and OCN were determined using the SYBR Premix Ex Taq II kit (Takara) on a 7500 sequence detection system (Applied Biosystems, Foster City, CA), according to the manufacturer's protocol.

    Article Title: Substrate Stiffness Regulates the Osteogenesis of PDLSCs Via ERK-Mediated YAP Nuclear Translocation.
    Article Snippet: .. Complementary DNA (cDNA) synthesis was then synthesized with the PrimeScript RT kit (Takara, Tokyo, Japan) and a reverse transcription instrument, the S1000 (Bio-Rad, Hercules, CA). .. The transcriptional levels of the genes Runx2 and OCN were determined using the SYBR Premix Ex Taq II kit (Takara) on a 7500 sequence detection system (Applied Biosystems, Foster City, CA), according to the manufacturer’s protocol.

    Reverse Transcription:

    Article Title: Substrate Stiffness Regulates the Osteogenesis of PDLSCs Via ERK-Mediated YAP Nuclear Translocation
    Article Snippet: .. Complementary DNA (cDNA) synthesis was then synthesized with the PrimeScript RT kit (Takara, Tokyo, Japan) and a reverse transcription instrument, the S1000 (Bio-Rad, Hercules, CA). .. The transcriptional levels of the genes Runx2 and OCN were determined using the SYBR Premix Ex Taq II kit (Takara) on a 7500 sequence detection system (Applied Biosystems, Foster City, CA), according to the manufacturer's protocol.

    Article Title: Substrate Stiffness Regulates the Osteogenesis of PDLSCs Via ERK-Mediated YAP Nuclear Translocation.
    Article Snippet: .. Complementary DNA (cDNA) synthesis was then synthesized with the PrimeScript RT kit (Takara, Tokyo, Japan) and a reverse transcription instrument, the S1000 (Bio-Rad, Hercules, CA). .. The transcriptional levels of the genes Runx2 and OCN were determined using the SYBR Premix Ex Taq II kit (Takara) on a 7500 sequence detection system (Applied Biosystems, Foster City, CA), according to the manufacturer’s protocol.

    Polymerase Chain Reaction:

    Article Title: Endophytic bacteria diversity in different tissues of Polygala sibirica Linnaeus and Polygala crotalarioides Buchanan-Hamilton ex Candolle
    Article Snippet: The purity and concentration of the genomic DNA were assessed by using a NanodropTM One (Thermo Fisher Scienti c, MA, USA). .. Using genomic DNA as the template, PCR ampli cation was performed, the hypervariable region V5-V7 of the bacterial 16S rRNA gene were ampli ed with primer pairs 799F (5’-AACMGGATTAGATACCCKG-3’) and 1193R (5’-ACGTCATCCCCACCTTCC-3’) by S1000 (BioRad, USA). .. The PCR mixtures contained 2 × Premix Taq buffer 25 μL, forward primer (10 μM) 1 μL, reverse primer (10 μM) 1 μL, template DNA 50 ng, nally ddH2O up to 50 μL (EX Taq Version 2.0 plus dye, Takara Biotechnology Dalian Co. Ltd., China).

    Article Title: Changes in the Arbuscular Mycorrhizal Fungal Community in the Roots of Eucalyptus grandis Plantations at Different Ages in Southern Jiangxi, China
    Article Snippet: PCR reactions, containing 25 μL 2x Premix Taq, 1 μL each primer (10 mM), and 3 μL DNA (20 ng/μL) template in a volume of 50 μL, were amplified by thermocycling: 5 min at 94 °C for initialization; 30 cycles of 30 s denaturation at 94 °C, 30 s annealing at 52 °C, and 30 s extension at 72 °C; followed by 10 min final elongation at 72 °C. .. Three replicates per sample and each PCR product of the same sample were mixed, with the PCR instrument BioRad S1000. ..

    Article Title: A one-pot loop-mediated isothermal amplification platform using fluorescent gold nanoclusters for rapid and naked-eye pathogen detection.
    Article Snippet: Loop-mediated isothermal amplification (LAMP) is a rapid and sensitive nucleic acid testing method for pathogen detection, yet the absence of a straightforward readout strategy remains challenging.. We've successfully designed polyethyleneimine-stabilized gold nanoclusters (PEI-AuNCs) as a cationic AuNCs indicator tailored for distinguishing LAMP results, enabling direct visual inspection under UV light.. Positive LAMP reactions with PEIAuNCs, in combination with magnesium pyrophosphate crystals, yield red-fluorescent bulk precipitates visible to the naked eye.

    Nucleic Acid Electrophoresis:

    Article Title: A one-pot loop-mediated isothermal amplification platform using fluorescent gold nanoclusters for rapid and naked-eye pathogen detection.
    Article Snippet: Loop-mediated isothermal amplification (LAMP) is a rapid and sensitive nucleic acid testing method for pathogen detection, yet the absence of a straightforward readout strategy remains challenging.. We've successfully designed polyethyleneimine-stabilized gold nanoclusters (PEI-AuNCs) as a cationic AuNCs indicator tailored for distinguishing LAMP results, enabling direct visual inspection under UV light.. Positive LAMP reactions with PEIAuNCs, in combination with magnesium pyrophosphate crystals, yield red-fluorescent bulk precipitates visible to the naked eye.



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