Journal: Scientific Reports

Article Title: RO8191, a new compound for initiating embryo implantation in mice

doi: 10.1038/s41598-025-18471-3

Figure Lengend Snippet: RO8191 induces embryo implantation in delayed implantation (DI) model mice. ( A ) The experimental procedure for the artificial DI mouse. Ovariectomy (OVX) and subcutaneous administration of siliconized medroxyprogesterone acetate (MPA) were performed on D3. A single injection of oil or RO8191 was performed on D7. Females were euthanized and dissected on D10. ( B ) Representative images of the gross uterine morphology. Blastocysts were recovered from oil-treated mice (white arrowheads). Implantation sites (yellow arrowheads on right panel) were visible in DI mice after RO8191 treatment. Scale bars: 1 cm for uterine images and 200 μm for blastocysts. ( C , D ) Implantation rate ( C ) and the number of implantation sites ( D ) in the oil and RO8191-treated DI mice. *Significantly different ( p < 0.0001). ( E ) Representative histological image of uterine cross-sections of implantation sites on D10 after RO8191 treatment in DI mice. Right panels are higher magnification of left panels indicated by yellow line. Lower panels indicated abnormal embryo development and decidual reaction. Scale bars: 100 μm. dec: decidua; em: embryo.

Article Snippet: The RO8191 ( T22142 , TargetMol, Boston, MA, USA, or SML1200, Sigma-Aldrich, St. Louis, MO, USA) was dissolved in sesame oil (196-15385, FujiFilm Wako Pure Chemicals, or S3547, Sigma-Aldrich), and a single intraperitoneal (i.p.) injection of RO8191 (400 μg/head) or sesame oil was performed at 1300 h on D7.

Techniques: Injection

Journal: Scientific Reports

Article Title: RO8191, a new compound for initiating embryo implantation in mice

doi: 10.1038/s41598-025-18471-3

Figure Lengend Snippet: RO8191 induces decidual reaction in mice with genetic implantation failure. ( A ) The experimental procedure for the genetically modified mice. For control, a single oil injection was performed on D4. For cKOs, a single injection of oil or RO8191 was performed on D4. Females were euthanized and dissected on D7. ( B – D ) Uterine morphology of cKOs mice after the injection of oil or RO8191. The white arrowheads indicate the presumptive implantation sites, including uterine swelling. Scale bars: 1 cm. ( E ) The implantation rate in cKOs mice after oil or RO8191 administration. ( F ) The number of implantation sites in cKOs mice after the injection of oil or RO8191. ( G ) The size of implantation sites in cKOs mice after oil or RO8191 administration. *Significantly different ( p < 0.05).

Article Snippet: The RO8191 ( T22142 , TargetMol, Boston, MA, USA, or SML1200, Sigma-Aldrich, St. Louis, MO, USA) was dissolved in sesame oil (196-15385, FujiFilm Wako Pure Chemicals, or S3547, Sigma-Aldrich), and a single intraperitoneal (i.p.) injection of RO8191 (400 μg/head) or sesame oil was performed at 1300 h on D7.

Techniques: Genetically Modified, Control, Injection

Journal: Scientific Reports

Article Title: RO8191, a new compound for initiating embryo implantation in mice

doi: 10.1038/s41598-025-18471-3

Figure Lengend Snippet: RO8191 activates STAT3, but not STAT1, in the endometrium. ( A ) Western blot analysis of STAT3 and phospho-STAT3 (p-STAT3) proteins after RO8191 treatment. The predicted band sizes are 89 kDa (STAT3α), 69 kDa (STAT3β), and 66 kDa (p-STAT3). ( B ) Western blot analysis of STAT1 and p-STAT1 after RO8191 treatment. A549 cells treated with IFN-γ were loaded as a control. The predicted band sizes are 91 kDa (STAT1α and p-STAT1α), 84 kDa (STAT1β and p-STAT1β). Blot images were obtained from one gel, and dividing lines indicate their separate origins. LE and ST protein lysates were pooled from at least three different individuals. ( C ) Representative immunohistochemical images of p-STAT3 expression in the uterus after RO8191 treatment. E2-treated uterus is shown as a control. Specimens were prepared at least three different individuals (two individuals in the case of E2). Scale bar: 100 μm. le: luminal epithelium; st: stroma.

Article Snippet: The RO8191 ( T22142 , TargetMol, Boston, MA, USA, or SML1200, Sigma-Aldrich, St. Louis, MO, USA) was dissolved in sesame oil (196-15385, FujiFilm Wako Pure Chemicals, or S3547, Sigma-Aldrich), and a single intraperitoneal (i.p.) injection of RO8191 (400 μg/head) or sesame oil was performed at 1300 h on D7.

Techniques: Western Blot, Control, Immunohistochemical staining, Expressing

Journal: Scientific Reports

Article Title: RO8191, a new compound for initiating embryo implantation in mice

doi: 10.1038/s41598-025-18471-3

Figure Lengend Snippet: Effect of RO8191 on the STAT3 signaling pathway in knockout mice. ( A ) Histological characterization of uterine cross-sections from presumed implantation sites on D7. Images a’-d’ are higher magnifications of images a-d, with further magnification in the yellow frames in b’ and c’. Scale bars: 100 μm. dec: decidua; le: luminal epithelium; em: embryo; bv: blood vessels; hem: hemorrhage; li: leukocyte infiltration. ( B ) Representative images of alkaline phosphatase (ALPL) expression. Scale bar: 100 μm. em: embryo; le: luminal epithelium; st: stroma. ( C ) The experimental procedure for maintaining pregnancy in Lifr cKO mice. A single injection of RO8191 was performed on D4, and females underwent cesarean section (CS) on D20-22. A single injection of RO8191 was sufficient to establish pregnancy in Lifr cKO mice. Representative images show an Lifr cKO female on D15 and the offspring obtained by CS. ( D ) Representative images of p-STAT3 in Gp130 cKO and Lifr cKO uterus 6 h after RO8191 treatment (D4 1600 h). Scale bar: 100 μm. le: luminal epithelium; st: stroma.

Article Snippet: The RO8191 ( T22142 , TargetMol, Boston, MA, USA, or SML1200, Sigma-Aldrich, St. Louis, MO, USA) was dissolved in sesame oil (196-15385, FujiFilm Wako Pure Chemicals, or S3547, Sigma-Aldrich), and a single intraperitoneal (i.p.) injection of RO8191 (400 μg/head) or sesame oil was performed at 1300 h on D7.

Techniques: Knock-Out, Expressing, Injection