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rmif  (ProSpec)

 
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    ProSpec rmif
    Rmif, supplied by ProSpec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rmif/product/ProSpec
    Average 90 stars, based on 1 article reviews
    rmif - by Bioz Stars, 2026-04
    90/100 stars

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    RAW264.7 cells pretreated with ISO-1 (10-50μmol/L) and stimulated with LPS/IFN-γ for 24h. A , Western blot images and quantification of MIF, iNOS and IL-18 in RAW264.7 cells. B , Representative flow cytometry charts of RAW264.7 cells in each group and quantification of <t>M1</t> <t>macrophages</t> (CD86+CD206-). C , Cytotoxic effects of <t>rMIF</t> (0-1000ng/ml) on RAW264.7 cells assessed by CCK8 assay. D , Administration of 0-500 ng/ml rMIF stimulated RAW264.7 cells for 15min-24h. Western blot was used to analyze the expression of iNOS and IL-18 proteins, and the protein expression of each group was quantified using the 0ng/ml group as a control.
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    RAW264.7 cells pretreated with ISO-1 (10-50μmol/L) and stimulated with LPS/IFN-γ for 24h. A , Western blot images and quantification of MIF, iNOS and IL-18 in RAW264.7 cells. B , Representative flow cytometry charts of RAW264.7 cells in each group and quantification of <t>M1</t> <t>macrophages</t> (CD86+CD206-). C , Cytotoxic effects of <t>rMIF</t> (0-1000ng/ml) on RAW264.7 cells assessed by CCK8 assay. D , Administration of 0-500 ng/ml rMIF stimulated RAW264.7 cells for 15min-24h. Western blot was used to analyze the expression of iNOS and IL-18 proteins, and the protein expression of each group was quantified using the 0ng/ml group as a control.
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    RAW264.7 cells pretreated with ISO-1 (10-50μmol/L) and stimulated with LPS/IFN-γ for 24h. A , Western blot images and quantification of MIF, iNOS and IL-18 in RAW264.7 cells. B , Representative flow cytometry charts of RAW264.7 cells in each group and quantification of <t>M1</t> <t>macrophages</t> (CD86+CD206-). C , Cytotoxic effects of <t>rMIF</t> (0-1000ng/ml) on RAW264.7 cells assessed by CCK8 assay. D , Administration of 0-500 ng/ml rMIF stimulated RAW264.7 cells for 15min-24h. Western blot was used to analyze the expression of iNOS and IL-18 proteins, and the protein expression of each group was quantified using the 0ng/ml group as a control.
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    Image Search Results


    RAW264.7 cells pretreated with ISO-1 (10-50μmol/L) and stimulated with LPS/IFN-γ for 24h. A , Western blot images and quantification of MIF, iNOS and IL-18 in RAW264.7 cells. B , Representative flow cytometry charts of RAW264.7 cells in each group and quantification of M1 macrophages (CD86+CD206-). C , Cytotoxic effects of rMIF (0-1000ng/ml) on RAW264.7 cells assessed by CCK8 assay. D , Administration of 0-500 ng/ml rMIF stimulated RAW264.7 cells for 15min-24h. Western blot was used to analyze the expression of iNOS and IL-18 proteins, and the protein expression of each group was quantified using the 0ng/ml group as a control.

    Journal: bioRxiv

    Article Title: MIF Regulates M1 Macrophage Polarization via CD74/CXCR2/JNK Pathway and Mediates Aortic Dissection in Mice

    doi: 10.1101/2023.10.26.564292

    Figure Lengend Snippet: RAW264.7 cells pretreated with ISO-1 (10-50μmol/L) and stimulated with LPS/IFN-γ for 24h. A , Western blot images and quantification of MIF, iNOS and IL-18 in RAW264.7 cells. B , Representative flow cytometry charts of RAW264.7 cells in each group and quantification of M1 macrophages (CD86+CD206-). C , Cytotoxic effects of rMIF (0-1000ng/ml) on RAW264.7 cells assessed by CCK8 assay. D , Administration of 0-500 ng/ml rMIF stimulated RAW264.7 cells for 15min-24h. Western blot was used to analyze the expression of iNOS and IL-18 proteins, and the protein expression of each group was quantified using the 0ng/ml group as a control.

    Article Snippet: Macrophages were stimulated with rMIF (MCE, USA) at concentrations of 10, 50, 100 and 500 ng/ml for 15 min, 30 min, 1h, 6h, 12h and 24h, and cells were collected for relevant experiments.

    Techniques: Western Blot, Flow Cytometry, CCK-8 Assay, Expressing

    A , Western blot images and quantification of iNOS and IL-18 in RAW264.7 cells treated by 50ng rMIF for 15min-24h. B , RT-qPCR of iNOS, TNF-α, IL-6 and Arg-1 in RAW264.7 cells treated by 0-500 ng/ml rMIF. C , Representative photographs and quantification of immunofluorescence staining of iNOS (green) and DAPI (blue) in RAW264.7 cells treated with MIF or LPS/IFN-γ. D , Representative flow cytometry charts of RAW264.7 cells treated with MIF or LPS/IFN-γ and quantification of M1 macrophages (CD86+CD206-).

    Journal: bioRxiv

    Article Title: MIF Regulates M1 Macrophage Polarization via CD74/CXCR2/JNK Pathway and Mediates Aortic Dissection in Mice

    doi: 10.1101/2023.10.26.564292

    Figure Lengend Snippet: A , Western blot images and quantification of iNOS and IL-18 in RAW264.7 cells treated by 50ng rMIF for 15min-24h. B , RT-qPCR of iNOS, TNF-α, IL-6 and Arg-1 in RAW264.7 cells treated by 0-500 ng/ml rMIF. C , Representative photographs and quantification of immunofluorescence staining of iNOS (green) and DAPI (blue) in RAW264.7 cells treated with MIF or LPS/IFN-γ. D , Representative flow cytometry charts of RAW264.7 cells treated with MIF or LPS/IFN-γ and quantification of M1 macrophages (CD86+CD206-).

    Article Snippet: Macrophages were stimulated with rMIF (MCE, USA) at concentrations of 10, 50, 100 and 500 ng/ml for 15 min, 30 min, 1h, 6h, 12h and 24h, and cells were collected for relevant experiments.

    Techniques: Western Blot, Quantitative RT-PCR, Immunofluorescence, Staining, Flow Cytometry