Journal: bioRxiv
Article Title: Creating bacterial genomic diversity through large-scale reconfigurations reveals phenotype robustness to organizational genome change
doi: 10.64898/2026.01.06.698047
Figure Lengend Snippet: ( A ) Engineering design of V. natriegens strain Vn14 chromosome 1. The outer circle indicates the genomic positions (Mbp) of loxPsym integrations, the chromosome replication origin ( oriC ), terminus ( ter ), and selection marker. The central circle shows the 14 loxPsym integrations (I to XIV) interspacing 14 chromosome segments represented as pentagons (1 to 14). Each chromosome segment is uniquely colored, and the direction of the pentagon represents the orientation. The inner circle indicates the size (Mbp) of each chromosomal segment. ( B ) Growth comparison of wild-type V. natriegens (Vn) and strain Vn14. Generation time (GT) results are presented as percentage of variation (%ΔGT) of the median with 95% CI relative to Vn (n = 12). ( C ) Visualization of Vn and Vn14 bacteria. ( D ) Fitness testing of Vn and Vn14 on LB3 agar. Serial 10-fold dilutions at 24 h.
Article Snippet: Wild-type V. natriegens Rif R (ATCC 14048) was used as the base strain in this study.
Techniques: Selection, Marker, Comparison, Bacteria