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recombinant human trail rhtrail cat# 310-04  (PeproTech)


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    Structured Review

    PeproTech recombinant human trail rhtrail cat# 310-04
    Recombinant Human Trail Rhtrail Cat# 310 04, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rhtrail/m+csf+cat+310+01+1mg/pm40247082-48-0-14
    Average 90 stars, based on 1 article reviews
    recombinant human trail rhtrail cat# 310-04 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: MSC-Delivered Soluble TRAIL and Paclitaxel as Novel Combinatory Treatment for Pancreatic Adenocarcinoma.
    Article Snippet: In the MTS assay (CellTiter 96 AQueous One Solution Cell Proliferation Assay, Promega Corporation, Madison, WI, USA), for each tested sample and condition, 5000 cells were seeded in a 96-wells plate. .. The following day, medium was replaced with sTRAIL-containing supernatant or AD-MSC EMPTY conditioned medium containing the appropriate concentration of rhTRAIL/Apo2 Ligand (rhTRAIL, Peprotech Inc., Rocky Hill, NJ, USA) (10, 20, 50, 100, 200, 500, 1000 and 10,000 ng/mL). .. After 24 h, the plate was analysed with Multiskan FC Microplate Photometer (Thermo Fisher Scientific Inc.) according to the manufacturer’s recommendations.

    Staining:

    Article Title: TRAIL responses are enhanced by nuclear export inhibition in osteosarcoma.
    Article Snippet: .. 96-well plates were seeded with 2.0 104 cells/well (MG63 & U2-OS) or 4.0 104 cells/well (Saos-2) and allowed to settle overnight prior to treatment with 0e50 ng/mL rhTRAIL (PeproTech EC Ltd, London, UK) or 0e50 ng/mL anti-DR5 monoclonal antibody (Mab 631, R&D Systems, Abingdon, UK) and/or 0e20 nM Leptomycin B (Enzo Life Sciences, Exeter, UK) for 24 h. Induction of apoptosis was assessed by staining cells with 10 mg/mL Hoechst 33342 and 10 mg/mL Propidium Iodide (PI; Sigma, Poole, UK) at 37 C for 30min. .. Cultures were visualised using an Olympus IX81 inverted fluorescent microscope and images captured using Cell-F software (Olympus, Tokyo, Japan).

    Article Title: Soluble TRAIL Armed Human MSC As Gene Therapy For Pancreatic Cancer
    Article Snippet: .. Activity of sTRAIL produced by AD-MSC on PDAC cells was evaluated after 24 hours by FACS using propidium iodide (PI; 50 μg/ml; Sigma Aldrich) supravital staining. rhTRAIL (1 μg/ml; Peprotech Inc.) was used as positive control. ..

    Activity Assay:

    Article Title: Soluble TRAIL Armed Human MSC As Gene Therapy For Pancreatic Cancer
    Article Snippet: .. Activity of sTRAIL produced by AD-MSC on PDAC cells was evaluated after 24 hours by FACS using propidium iodide (PI; 50 μg/ml; Sigma Aldrich) supravital staining. rhTRAIL (1 μg/ml; Peprotech Inc.) was used as positive control. ..

    Produced:

    Article Title: Soluble TRAIL Armed Human MSC As Gene Therapy For Pancreatic Cancer
    Article Snippet: .. Activity of sTRAIL produced by AD-MSC on PDAC cells was evaluated after 24 hours by FACS using propidium iodide (PI; 50 μg/ml; Sigma Aldrich) supravital staining. rhTRAIL (1 μg/ml; Peprotech Inc.) was used as positive control. ..

    FACS:

    Article Title: Soluble TRAIL Armed Human MSC As Gene Therapy For Pancreatic Cancer
    Article Snippet: .. Activity of sTRAIL produced by AD-MSC on PDAC cells was evaluated after 24 hours by FACS using propidium iodide (PI; 50 μg/ml; Sigma Aldrich) supravital staining. rhTRAIL (1 μg/ml; Peprotech Inc.) was used as positive control. ..

    Positive Control:

    Article Title: Soluble TRAIL Armed Human MSC As Gene Therapy For Pancreatic Cancer
    Article Snippet: .. Activity of sTRAIL produced by AD-MSC on PDAC cells was evaluated after 24 hours by FACS using propidium iodide (PI; 50 μg/ml; Sigma Aldrich) supravital staining. rhTRAIL (1 μg/ml; Peprotech Inc.) was used as positive control. ..

    Recombinant:

    Article Title: Combined effects of XAF1 and TRAIL on the apoptosis of lung adenocarcinoma cells
    Article Snippet: .. Reagents Recombinant virus Ad5/F35-XAF1 and controlled virus Ad5/F35-Null were constructed by Shanghai R&S Biotechnology Co., Ltd. (Shanghai, China) and stored in a laboratory (Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China). rhTRAIL was bought from Peprotech, Inc. (Rocky Hill, NJ, USA). .. Reverse transcription system was provided by Promega Corp. (Madison, WI, USA).

    Article Title: Combined effects of XAF1 and TRAIL on the apoptosis of lung adenocarcinoma cells
    Article Snippet: .. Recombinant virus Ad5/F35-XAF1 and controlled virus Ad5/F35-Null were constructed by Shanghai R&S Biotechnology Co., Ltd. (Shanghai, China) and stored in a laboratory (Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China). rhTRAIL was bought from Peprotech, Inc. (Rocky Hill, NJ, USA). .. Reverse transcription system was provided by Promega Corp. (Madison, WI, USA).

    Virus:

    Article Title: Combined effects of XAF1 and TRAIL on the apoptosis of lung adenocarcinoma cells
    Article Snippet: .. Reagents Recombinant virus Ad5/F35-XAF1 and controlled virus Ad5/F35-Null were constructed by Shanghai R&S Biotechnology Co., Ltd. (Shanghai, China) and stored in a laboratory (Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China). rhTRAIL was bought from Peprotech, Inc. (Rocky Hill, NJ, USA). .. Reverse transcription system was provided by Promega Corp. (Madison, WI, USA).

    Article Title: Combined effects of XAF1 and TRAIL on the apoptosis of lung adenocarcinoma cells
    Article Snippet: .. Recombinant virus Ad5/F35-XAF1 and controlled virus Ad5/F35-Null were constructed by Shanghai R&S Biotechnology Co., Ltd. (Shanghai, China) and stored in a laboratory (Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China). rhTRAIL was bought from Peprotech, Inc. (Rocky Hill, NJ, USA). .. Reverse transcription system was provided by Promega Corp. (Madison, WI, USA).

    Construct:

    Article Title: Combined effects of XAF1 and TRAIL on the apoptosis of lung adenocarcinoma cells
    Article Snippet: .. Reagents Recombinant virus Ad5/F35-XAF1 and controlled virus Ad5/F35-Null were constructed by Shanghai R&S Biotechnology Co., Ltd. (Shanghai, China) and stored in a laboratory (Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China). rhTRAIL was bought from Peprotech, Inc. (Rocky Hill, NJ, USA). .. Reverse transcription system was provided by Promega Corp. (Madison, WI, USA).

    Article Title: Combined effects of XAF1 and TRAIL on the apoptosis of lung adenocarcinoma cells
    Article Snippet: .. Recombinant virus Ad5/F35-XAF1 and controlled virus Ad5/F35-Null were constructed by Shanghai R&S Biotechnology Co., Ltd. (Shanghai, China) and stored in a laboratory (Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China). rhTRAIL was bought from Peprotech, Inc. (Rocky Hill, NJ, USA). .. Reverse transcription system was provided by Promega Corp. (Madison, WI, USA).



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    Figure 1. <t>rhTRAIL</t> exposure effect alone and in combination with WT HSV-1 virus on HNSCC cell lines and the normal cell line, HaCaT. Treatment was performed with rhTRAIL at 100 ng/mL and in combination with WT HSV-1 virus at MOI 0f. 0.2 and 1.0. Cell viability analysis after 48 (A) and 72 h (B) of exposure. Results are expressed by mean percentage ± standard deviation of cell viability relative to PBS 1X (considering 100% viability). Data represent the average of three independent assays performed in triplicate. The asterisks indicate statistical significance (*P < 0.05; **P < 0.01; ***P < 0.001) in the comparison with the experimental groups by non-normal distribution Kruskal–Wallis test.
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    Image Search Results


    Figure 1. rhTRAIL exposure effect alone and in combination with WT HSV-1 virus on HNSCC cell lines and the normal cell line, HaCaT. Treatment was performed with rhTRAIL at 100 ng/mL and in combination with WT HSV-1 virus at MOI 0f. 0.2 and 1.0. Cell viability analysis after 48 (A) and 72 h (B) of exposure. Results are expressed by mean percentage ± standard deviation of cell viability relative to PBS 1X (considering 100% viability). Data represent the average of three independent assays performed in triplicate. The asterisks indicate statistical significance (*P < 0.05; **P < 0.01; ***P < 0.001) in the comparison with the experimental groups by non-normal distribution Kruskal–Wallis test.

    Journal: Scientific reports

    Article Title: Combined effect of the pro-apoptotic rhTRAIL protein and HSV-1 virus in head and neck cancer cell lines.

    doi: 10.1038/s41598-023-44888-9

    Figure Lengend Snippet: Figure 1. rhTRAIL exposure effect alone and in combination with WT HSV-1 virus on HNSCC cell lines and the normal cell line, HaCaT. Treatment was performed with rhTRAIL at 100 ng/mL and in combination with WT HSV-1 virus at MOI 0f. 0.2 and 1.0. Cell viability analysis after 48 (A) and 72 h (B) of exposure. Results are expressed by mean percentage ± standard deviation of cell viability relative to PBS 1X (considering 100% viability). Data represent the average of three independent assays performed in triplicate. The asterisks indicate statistical significance (*P < 0.05; **P < 0.01; ***P < 0.001) in the comparison with the experimental groups by non-normal distribution Kruskal–Wallis test.

    Article Snippet: :(0123456789) Scientific Reports | (2023) 13:18023 | https://doi.org/10.1038/s41598-023-44888-9 treated with rhTRAIL (#375-TL, R&D Systems, Minneapolis, MN, EUA) at 100 ng/mL, HSV-1 MOI 0.2 and/ or HSV-1 MOI 1.

    Techniques: Virus, Standard Deviation, Comparison

    Figure 2. Expression analysis of apoptosis-associated proteins in the HCB289 cell line after 24 h of exposure with rhTRAIL ligand, WT HSV-1 and in combination. (A) and (B) detection and quantification of proteins after treatment. Results are expressed as mean percentage ± standard deviation of relative expression of cleaved protein relative to control (considering 100% expression) and normalized by the portion of total protein (non- cleaved). Data represent the average of three independent assays. The asterisks indicate statistical significance (*P < 0.05; **P < 0.01; ***P < 0.001) in the comparison with the experimental groups by one-way ANOVA. Raw blots are presented in Supplementary Data 1.

    Journal: Scientific reports

    Article Title: Combined effect of the pro-apoptotic rhTRAIL protein and HSV-1 virus in head and neck cancer cell lines.

    doi: 10.1038/s41598-023-44888-9

    Figure Lengend Snippet: Figure 2. Expression analysis of apoptosis-associated proteins in the HCB289 cell line after 24 h of exposure with rhTRAIL ligand, WT HSV-1 and in combination. (A) and (B) detection and quantification of proteins after treatment. Results are expressed as mean percentage ± standard deviation of relative expression of cleaved protein relative to control (considering 100% expression) and normalized by the portion of total protein (non- cleaved). Data represent the average of three independent assays. The asterisks indicate statistical significance (*P < 0.05; **P < 0.01; ***P < 0.001) in the comparison with the experimental groups by one-way ANOVA. Raw blots are presented in Supplementary Data 1.

    Article Snippet: :(0123456789) Scientific Reports | (2023) 13:18023 | https://doi.org/10.1038/s41598-023-44888-9 treated with rhTRAIL (#375-TL, R&D Systems, Minneapolis, MN, EUA) at 100 ng/mL, HSV-1 MOI 0.2 and/ or HSV-1 MOI 1.

    Techniques: Expressing, Standard Deviation, Control, Comparison

    Figure 3. Expression analysis of apoptosis-associated proteins in the UD-SCC-2 cell line after 24 h of exposure with rhTRAIL ligand, WT HSV-1 and in combination. (A) and (B) detection and quantification of proteins after treatment. Results are expressed as mean percentage ± standard deviation of relative expression of cleaved protein relative to control (considering 100% expression) and normalized by the portion of total protein (non- cleaved). Data represent the average of three independent assays. The asterisks indicate statistical significance (*P < 0.05; **P < 0.01; ***P < 0.001) in the comparison with the experimental groups by one-way ANOVA. Raw blots are presented in Supplementary Data 2.

    Journal: Scientific reports

    Article Title: Combined effect of the pro-apoptotic rhTRAIL protein and HSV-1 virus in head and neck cancer cell lines.

    doi: 10.1038/s41598-023-44888-9

    Figure Lengend Snippet: Figure 3. Expression analysis of apoptosis-associated proteins in the UD-SCC-2 cell line after 24 h of exposure with rhTRAIL ligand, WT HSV-1 and in combination. (A) and (B) detection and quantification of proteins after treatment. Results are expressed as mean percentage ± standard deviation of relative expression of cleaved protein relative to control (considering 100% expression) and normalized by the portion of total protein (non- cleaved). Data represent the average of three independent assays. The asterisks indicate statistical significance (*P < 0.05; **P < 0.01; ***P < 0.001) in the comparison with the experimental groups by one-way ANOVA. Raw blots are presented in Supplementary Data 2.

    Article Snippet: :(0123456789) Scientific Reports | (2023) 13:18023 | https://doi.org/10.1038/s41598-023-44888-9 treated with rhTRAIL (#375-TL, R&D Systems, Minneapolis, MN, EUA) at 100 ng/mL, HSV-1 MOI 0.2 and/ or HSV-1 MOI 1.

    Techniques: Expressing, Standard Deviation, Control, Comparison

    Figure 4. Expression analysis of apoptosis-associated proteins in the HCB289 cell line after 48 h of exposure with rhTRAIL ligand, WT HSV-1 and in combination. (A) and (B) detection and quantification of proteins after treatment. Results are expressed as mean percentage ± standard deviation of relative expression of cleaved protein relative to control (considering 100% expression) and normalized by the portion of total protein (non- cleaved). Data represent the average of two independent assays. The asterisks indicate statistical significance (*P < 0.05) in the comparison with the experimental groups by one-way ANOVA. Raw blots are presented in Supplementary Data 3.

    Journal: Scientific reports

    Article Title: Combined effect of the pro-apoptotic rhTRAIL protein and HSV-1 virus in head and neck cancer cell lines.

    doi: 10.1038/s41598-023-44888-9

    Figure Lengend Snippet: Figure 4. Expression analysis of apoptosis-associated proteins in the HCB289 cell line after 48 h of exposure with rhTRAIL ligand, WT HSV-1 and in combination. (A) and (B) detection and quantification of proteins after treatment. Results are expressed as mean percentage ± standard deviation of relative expression of cleaved protein relative to control (considering 100% expression) and normalized by the portion of total protein (non- cleaved). Data represent the average of two independent assays. The asterisks indicate statistical significance (*P < 0.05) in the comparison with the experimental groups by one-way ANOVA. Raw blots are presented in Supplementary Data 3.

    Article Snippet: :(0123456789) Scientific Reports | (2023) 13:18023 | https://doi.org/10.1038/s41598-023-44888-9 treated with rhTRAIL (#375-TL, R&D Systems, Minneapolis, MN, EUA) at 100 ng/mL, HSV-1 MOI 0.2 and/ or HSV-1 MOI 1.

    Techniques: Expressing, Standard Deviation, Control, Comparison

    Figure 5. Expression analysis of apoptosis-associated proteins in the UD-SCC-2 cell line after 48 h of exposure with rhTRAIL ligand, WT HSV-1 and in combination. (A) and (B) detection and quantification of proteins after treatment. Results are expressed as mean percentage ± standard deviation of relative expression of cleaved protein relative to control (considering 100% expression) and normalized by the portion of total protein (non- cleaved). Data represent the average of two independent assays. The asterisks indicate statistical significance (*P < 0.05) in the comparison with the experimental groups by one-way ANOVA. Raw blots are presented in Supplementary Data 4.

    Journal: Scientific reports

    Article Title: Combined effect of the pro-apoptotic rhTRAIL protein and HSV-1 virus in head and neck cancer cell lines.

    doi: 10.1038/s41598-023-44888-9

    Figure Lengend Snippet: Figure 5. Expression analysis of apoptosis-associated proteins in the UD-SCC-2 cell line after 48 h of exposure with rhTRAIL ligand, WT HSV-1 and in combination. (A) and (B) detection and quantification of proteins after treatment. Results are expressed as mean percentage ± standard deviation of relative expression of cleaved protein relative to control (considering 100% expression) and normalized by the portion of total protein (non- cleaved). Data represent the average of two independent assays. The asterisks indicate statistical significance (*P < 0.05) in the comparison with the experimental groups by one-way ANOVA. Raw blots are presented in Supplementary Data 4.

    Article Snippet: :(0123456789) Scientific Reports | (2023) 13:18023 | https://doi.org/10.1038/s41598-023-44888-9 treated with rhTRAIL (#375-TL, R&D Systems, Minneapolis, MN, EUA) at 100 ng/mL, HSV-1 MOI 0.2 and/ or HSV-1 MOI 1.

    Techniques: Expressing, Standard Deviation, Control, Comparison

    Figure 6. Flow cytometry apoptosis analysis after treatment with rhTRAIL ligand, WT HSV-1 and in combination. A and B) Analysis of HCB289 cell line after 24 and 48 h of treatment exposure. C and D) Analysis of UD-SCC-2 cell line after 24 and 48 h of treatment exposure. Results are expressed as mean percentage ± standard deviation of the cell death rate. Data represent the average of three independent assays. The asterisks indicate statistical significance (*P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.001) in the comparison with the experimental groups by one-way ANOVA.

    Journal: Scientific reports

    Article Title: Combined effect of the pro-apoptotic rhTRAIL protein and HSV-1 virus in head and neck cancer cell lines.

    doi: 10.1038/s41598-023-44888-9

    Figure Lengend Snippet: Figure 6. Flow cytometry apoptosis analysis after treatment with rhTRAIL ligand, WT HSV-1 and in combination. A and B) Analysis of HCB289 cell line after 24 and 48 h of treatment exposure. C and D) Analysis of UD-SCC-2 cell line after 24 and 48 h of treatment exposure. Results are expressed as mean percentage ± standard deviation of the cell death rate. Data represent the average of three independent assays. The asterisks indicate statistical significance (*P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.001) in the comparison with the experimental groups by one-way ANOVA.

    Article Snippet: :(0123456789) Scientific Reports | (2023) 13:18023 | https://doi.org/10.1038/s41598-023-44888-9 treated with rhTRAIL (#375-TL, R&D Systems, Minneapolis, MN, EUA) at 100 ng/mL, HSV-1 MOI 0.2 and/ or HSV-1 MOI 1.

    Techniques: Flow Cytometry, Standard Deviation, Comparison

    Figure 7. DR-5 expression analysis in the HCB289 cell line after treatment with rhTRAIL ligand, WT-HSV-1 and combination. (A) and (B) Detection and quantification of the receptor after 24 h of exposure treatment. (C) and (D) Detection and quantification of the receptor after 48 h of exposure treatment. Results are expressed as mean percentage ± standard deviation of relative expression of cleaved protein relative to control (considering 100% expression) and normalized by the protein α-tubulin. Data represent the average of two independent. Raw blots are presented in Supplementary Data 5.

    Journal: Scientific reports

    Article Title: Combined effect of the pro-apoptotic rhTRAIL protein and HSV-1 virus in head and neck cancer cell lines.

    doi: 10.1038/s41598-023-44888-9

    Figure Lengend Snippet: Figure 7. DR-5 expression analysis in the HCB289 cell line after treatment with rhTRAIL ligand, WT-HSV-1 and combination. (A) and (B) Detection and quantification of the receptor after 24 h of exposure treatment. (C) and (D) Detection and quantification of the receptor after 48 h of exposure treatment. Results are expressed as mean percentage ± standard deviation of relative expression of cleaved protein relative to control (considering 100% expression) and normalized by the protein α-tubulin. Data represent the average of two independent. Raw blots are presented in Supplementary Data 5.

    Article Snippet: :(0123456789) Scientific Reports | (2023) 13:18023 | https://doi.org/10.1038/s41598-023-44888-9 treated with rhTRAIL (#375-TL, R&D Systems, Minneapolis, MN, EUA) at 100 ng/mL, HSV-1 MOI 0.2 and/ or HSV-1 MOI 1.

    Techniques: Expressing, Standard Deviation, Control

    Figure 8. DR-5 expression analysis in the UD-SCC-2 cell line after treatment with rhTRAIL ligand, WT-HSV-1 and combination. (A) and (B) Detection and quantification of the receptor after 24 h of exposure treatment. (C) and (D) Detection and quantification of the receptor after 48 h of exposure treatment. Results are expressed as mean percentage ± standard deviation of relative expression of cleaved protein relative to control (considering 100% expression) and normalized by the protein α-tubulin. Data represent the average of two independent assays. Raw blots are presented in Supplementary Data 6.

    Journal: Scientific reports

    Article Title: Combined effect of the pro-apoptotic rhTRAIL protein and HSV-1 virus in head and neck cancer cell lines.

    doi: 10.1038/s41598-023-44888-9

    Figure Lengend Snippet: Figure 8. DR-5 expression analysis in the UD-SCC-2 cell line after treatment with rhTRAIL ligand, WT-HSV-1 and combination. (A) and (B) Detection and quantification of the receptor after 24 h of exposure treatment. (C) and (D) Detection and quantification of the receptor after 48 h of exposure treatment. Results are expressed as mean percentage ± standard deviation of relative expression of cleaved protein relative to control (considering 100% expression) and normalized by the protein α-tubulin. Data represent the average of two independent assays. Raw blots are presented in Supplementary Data 6.

    Article Snippet: :(0123456789) Scientific Reports | (2023) 13:18023 | https://doi.org/10.1038/s41598-023-44888-9 treated with rhTRAIL (#375-TL, R&D Systems, Minneapolis, MN, EUA) at 100 ng/mL, HSV-1 MOI 0.2 and/ or HSV-1 MOI 1.

    Techniques: Expressing, Standard Deviation, Control