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rhnoggin  (PeproTech)


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    PeproTech rhnoggin
    Rhnoggin, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rhnoggin/hnoggin/pm39716485-64-42-43
    Average 90 stars, based on 1 article reviews
    rhnoggin - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Targeting UBE2T suppresses breast cancer stemness through CBX6-mediated transcriptional repression of SOX2 and NANOG.
    Article Snippet: Breast cancer stem cells (BCSCs) are the main cause of breast cancer recurrence and metastasis.. While the ubiquitin-proteasome system contributes to the regulation of BCSC stemness, the underlying mechanisms remain unclear.. Here, we identified ubiquitin-conjugating enzyme E2T (UBE2T) as a pivotal ubiquitin enzyme regulating BCSC stemness through systemic screening assays, including single-cell RNA sequencing (scRNA-seq) and stemness-index analysis.

    Generated:

    Article Title: Organoid co-culture model of the human endometrium in a fully synthetic extracellular matrix enables the study of epithelial-stromal crosstalk.
    Article Snippet: Some cell cultures were treated with 500 nM synthetic progesterone medroxyprogesterone acetate (MPA, Sigma Aldrich), or the progesterone antagonist mifepristone (RU-486 10 mM). .. Primary endometrial epithelial organoids (EEOs) were generated from the primary epithelial fragments (p0) according to established protocols and maintained in endometrial epithelial organoid expansion medium (EEO medium).10,11 EEO media is modified from the protocols established by Turco et al.11 Briefly, EEO media was defined as the minimal set of components needed to establish organoid cultures expansion and was composed of advanced basal media cocktail11 (ABM, 1X); rhEGF (50 ng/mL, Corning); rhNoggin (100 ng/mL, Peprotech); rhRspondin-1 (200 ng/mL, R&D systems); rhFGF-10 (50 ng/mL, Peprotech); E2 (1nM); Nicotinamide (1mM); Insulin-Transferrin-Selenium (ITS, 1%, Invitrogen); N-acetyl-L-cysteine (1.25 mM); TGFB pathway inhibitor (A83-01, 500 nM, Peprotech); and Rock inhibitor Y-27632, (ROCKi, 10 mM, Tocris). ..

    Modification:

    Article Title: Organoid co-culture model of the human endometrium in a fully synthetic extracellular matrix enables the study of epithelial-stromal crosstalk.
    Article Snippet: Some cell cultures were treated with 500 nM synthetic progesterone medroxyprogesterone acetate (MPA, Sigma Aldrich), or the progesterone antagonist mifepristone (RU-486 10 mM). .. Primary endometrial epithelial organoids (EEOs) were generated from the primary epithelial fragments (p0) according to established protocols and maintained in endometrial epithelial organoid expansion medium (EEO medium).10,11 EEO media is modified from the protocols established by Turco et al.11 Briefly, EEO media was defined as the minimal set of components needed to establish organoid cultures expansion and was composed of advanced basal media cocktail11 (ABM, 1X); rhEGF (50 ng/mL, Corning); rhNoggin (100 ng/mL, Peprotech); rhRspondin-1 (200 ng/mL, R&D systems); rhFGF-10 (50 ng/mL, Peprotech); E2 (1nM); Nicotinamide (1mM); Insulin-Transferrin-Selenium (ITS, 1%, Invitrogen); N-acetyl-L-cysteine (1.25 mM); TGFB pathway inhibitor (A83-01, 500 nM, Peprotech); and Rock inhibitor Y-27632, (ROCKi, 10 mM, Tocris). ..

    Virus:

    Article Title: Pancreatic Cell Fate Determination Relies on Notch Ligand Trafficking by NFIA.
    Article Snippet: .. Cat: 127-025-160; RRID: AB_2338974 Bacterial and Virus Strains Mib1 MISSION shRNA Millipore Sigma Cat: SHCLNG-NM_144860 Mib1 shRNA Dharmacon Clones: V2LHS_35581 and V2LHS_35584 FUGW-NFIA-GFP B. Deneen, Baylor College of Medicine N/A NFIA tm1a (EUCOMM) Hmgu Mouse Genome Informatics MGI: 4458675 AAV-NEXT-V5 L. Alberi, Swiss Integrative Center for Human Health N/A pmaxGFP Lonza/Amaxa N/A Chemicals, Peptides, and Recombinant Proteins Tamoxifen Sigma Cat: T5648 EGF Sigma Cat: E2759-5X15MG FGF1 VWR International Cat: 89508-958 FGF10 R&D Systems Cat: 345-FG Heparin Sigma Cat: H3149-10KU R-Spondin1 R&D Systems Cat: 4645-RS-025 Y-27632 R&D Systems Cat: 1254-10 PMA Sigma Cat: P1585 CHIR-99021 Stemgent Cat: 04-0004-10 rhActivinA Peprotech Cat: 120-14E KGF Peprotech Cat: 100-19 RA Sigma Cat: R2625 SANT-1 Sigma Cat: S4572 rhNoggin Peprotech Cat: 120-10C Critical Commercial Assays TruSeq Stranded mRNA kit Illumina Cat: 20020594 Deposited Data Raw data files from RNA-seq This paper GEO: GSE86909 Experimental Models: Cell Lines H1 human ES cell line (WA01) WiCell Research Institute, Inc. RRID:CVCL_9771 Experimental Models: Organisms/Strains Mouse: Pdx1-cre Jackson Laboratory Stock: 024968 Mouse: Ptf1a-cre Jackson Laboratory Stock: 023329 Mouse: Sox9-creERt2 Jackson Laboratory Stock: 018829 Mouse: NFIA-KO R. Gronostajski, University of Buffalo Jacobs School of Medicine and Biomedical Sciences N/A Mouse: Ngn3-eGFP Mouse Genome Informatics MGI: 2651394 Mouse: CBF1:H2B-Venus Jackson Laboratory Stock: 020942 Oligonucleotides Primers for qPCR, ChIP-qPCR and genotyping PCR This paper see Table S1 Software and Algorithms ImageJ NIH https://imagej.nih.gov/ij/; RRID: SCR_003070 Adobe Photoshop CS5.1 Adobe RRID: SCR_014199 Microsoft Excel 2011 Microsoft RRID: SCR_016137 Prism 5 Graphpad RRID: SCR_002798 (Continued on next page) Cell Reports 25, 3811–3827.e1–e7, December 26, 2018 e2 ..

    shRNA:

    Article Title: Pancreatic Cell Fate Determination Relies on Notch Ligand Trafficking by NFIA.
    Article Snippet: .. Cat: 127-025-160; RRID: AB_2338974 Bacterial and Virus Strains Mib1 MISSION shRNA Millipore Sigma Cat: SHCLNG-NM_144860 Mib1 shRNA Dharmacon Clones: V2LHS_35581 and V2LHS_35584 FUGW-NFIA-GFP B. Deneen, Baylor College of Medicine N/A NFIA tm1a (EUCOMM) Hmgu Mouse Genome Informatics MGI: 4458675 AAV-NEXT-V5 L. Alberi, Swiss Integrative Center for Human Health N/A pmaxGFP Lonza/Amaxa N/A Chemicals, Peptides, and Recombinant Proteins Tamoxifen Sigma Cat: T5648 EGF Sigma Cat: E2759-5X15MG FGF1 VWR International Cat: 89508-958 FGF10 R&D Systems Cat: 345-FG Heparin Sigma Cat: H3149-10KU R-Spondin1 R&D Systems Cat: 4645-RS-025 Y-27632 R&D Systems Cat: 1254-10 PMA Sigma Cat: P1585 CHIR-99021 Stemgent Cat: 04-0004-10 rhActivinA Peprotech Cat: 120-14E KGF Peprotech Cat: 100-19 RA Sigma Cat: R2625 SANT-1 Sigma Cat: S4572 rhNoggin Peprotech Cat: 120-10C Critical Commercial Assays TruSeq Stranded mRNA kit Illumina Cat: 20020594 Deposited Data Raw data files from RNA-seq This paper GEO: GSE86909 Experimental Models: Cell Lines H1 human ES cell line (WA01) WiCell Research Institute, Inc. RRID:CVCL_9771 Experimental Models: Organisms/Strains Mouse: Pdx1-cre Jackson Laboratory Stock: 024968 Mouse: Ptf1a-cre Jackson Laboratory Stock: 023329 Mouse: Sox9-creERt2 Jackson Laboratory Stock: 018829 Mouse: NFIA-KO R. Gronostajski, University of Buffalo Jacobs School of Medicine and Biomedical Sciences N/A Mouse: Ngn3-eGFP Mouse Genome Informatics MGI: 2651394 Mouse: CBF1:H2B-Venus Jackson Laboratory Stock: 020942 Oligonucleotides Primers for qPCR, ChIP-qPCR and genotyping PCR This paper see Table S1 Software and Algorithms ImageJ NIH https://imagej.nih.gov/ij/; RRID: SCR_003070 Adobe Photoshop CS5.1 Adobe RRID: SCR_014199 Microsoft Excel 2011 Microsoft RRID: SCR_016137 Prism 5 Graphpad RRID: SCR_002798 (Continued on next page) Cell Reports 25, 3811–3827.e1–e7, December 26, 2018 e2 ..

    Clone Assay:

    Article Title: Pancreatic Cell Fate Determination Relies on Notch Ligand Trafficking by NFIA.
    Article Snippet: .. Cat: 127-025-160; RRID: AB_2338974 Bacterial and Virus Strains Mib1 MISSION shRNA Millipore Sigma Cat: SHCLNG-NM_144860 Mib1 shRNA Dharmacon Clones: V2LHS_35581 and V2LHS_35584 FUGW-NFIA-GFP B. Deneen, Baylor College of Medicine N/A NFIA tm1a (EUCOMM) Hmgu Mouse Genome Informatics MGI: 4458675 AAV-NEXT-V5 L. Alberi, Swiss Integrative Center for Human Health N/A pmaxGFP Lonza/Amaxa N/A Chemicals, Peptides, and Recombinant Proteins Tamoxifen Sigma Cat: T5648 EGF Sigma Cat: E2759-5X15MG FGF1 VWR International Cat: 89508-958 FGF10 R&D Systems Cat: 345-FG Heparin Sigma Cat: H3149-10KU R-Spondin1 R&D Systems Cat: 4645-RS-025 Y-27632 R&D Systems Cat: 1254-10 PMA Sigma Cat: P1585 CHIR-99021 Stemgent Cat: 04-0004-10 rhActivinA Peprotech Cat: 120-14E KGF Peprotech Cat: 100-19 RA Sigma Cat: R2625 SANT-1 Sigma Cat: S4572 rhNoggin Peprotech Cat: 120-10C Critical Commercial Assays TruSeq Stranded mRNA kit Illumina Cat: 20020594 Deposited Data Raw data files from RNA-seq This paper GEO: GSE86909 Experimental Models: Cell Lines H1 human ES cell line (WA01) WiCell Research Institute, Inc. RRID:CVCL_9771 Experimental Models: Organisms/Strains Mouse: Pdx1-cre Jackson Laboratory Stock: 024968 Mouse: Ptf1a-cre Jackson Laboratory Stock: 023329 Mouse: Sox9-creERt2 Jackson Laboratory Stock: 018829 Mouse: NFIA-KO R. Gronostajski, University of Buffalo Jacobs School of Medicine and Biomedical Sciences N/A Mouse: Ngn3-eGFP Mouse Genome Informatics MGI: 2651394 Mouse: CBF1:H2B-Venus Jackson Laboratory Stock: 020942 Oligonucleotides Primers for qPCR, ChIP-qPCR and genotyping PCR This paper see Table S1 Software and Algorithms ImageJ NIH https://imagej.nih.gov/ij/; RRID: SCR_003070 Adobe Photoshop CS5.1 Adobe RRID: SCR_014199 Microsoft Excel 2011 Microsoft RRID: SCR_016137 Prism 5 Graphpad RRID: SCR_002798 (Continued on next page) Cell Reports 25, 3811–3827.e1–e7, December 26, 2018 e2 ..

    Bioprocessing:

    Article Title: Pancreatic Cell Fate Determination Relies on Notch Ligand Trafficking by NFIA.
    Article Snippet: .. Cat: 127-025-160; RRID: AB_2338974 Bacterial and Virus Strains Mib1 MISSION shRNA Millipore Sigma Cat: SHCLNG-NM_144860 Mib1 shRNA Dharmacon Clones: V2LHS_35581 and V2LHS_35584 FUGW-NFIA-GFP B. Deneen, Baylor College of Medicine N/A NFIA tm1a (EUCOMM) Hmgu Mouse Genome Informatics MGI: 4458675 AAV-NEXT-V5 L. Alberi, Swiss Integrative Center for Human Health N/A pmaxGFP Lonza/Amaxa N/A Chemicals, Peptides, and Recombinant Proteins Tamoxifen Sigma Cat: T5648 EGF Sigma Cat: E2759-5X15MG FGF1 VWR International Cat: 89508-958 FGF10 R&D Systems Cat: 345-FG Heparin Sigma Cat: H3149-10KU R-Spondin1 R&D Systems Cat: 4645-RS-025 Y-27632 R&D Systems Cat: 1254-10 PMA Sigma Cat: P1585 CHIR-99021 Stemgent Cat: 04-0004-10 rhActivinA Peprotech Cat: 120-14E KGF Peprotech Cat: 100-19 RA Sigma Cat: R2625 SANT-1 Sigma Cat: S4572 rhNoggin Peprotech Cat: 120-10C Critical Commercial Assays TruSeq Stranded mRNA kit Illumina Cat: 20020594 Deposited Data Raw data files from RNA-seq This paper GEO: GSE86909 Experimental Models: Cell Lines H1 human ES cell line (WA01) WiCell Research Institute, Inc. RRID:CVCL_9771 Experimental Models: Organisms/Strains Mouse: Pdx1-cre Jackson Laboratory Stock: 024968 Mouse: Ptf1a-cre Jackson Laboratory Stock: 023329 Mouse: Sox9-creERt2 Jackson Laboratory Stock: 018829 Mouse: NFIA-KO R. Gronostajski, University of Buffalo Jacobs School of Medicine and Biomedical Sciences N/A Mouse: Ngn3-eGFP Mouse Genome Informatics MGI: 2651394 Mouse: CBF1:H2B-Venus Jackson Laboratory Stock: 020942 Oligonucleotides Primers for qPCR, ChIP-qPCR and genotyping PCR This paper see Table S1 Software and Algorithms ImageJ NIH https://imagej.nih.gov/ij/; RRID: SCR_003070 Adobe Photoshop CS5.1 Adobe RRID: SCR_014199 Microsoft Excel 2011 Microsoft RRID: SCR_016137 Prism 5 Graphpad RRID: SCR_002798 (Continued on next page) Cell Reports 25, 3811–3827.e1–e7, December 26, 2018 e2 ..

    Recombinant:

    Article Title: Pancreatic Cell Fate Determination Relies on Notch Ligand Trafficking by NFIA.
    Article Snippet: .. Cat: 127-025-160; RRID: AB_2338974 Bacterial and Virus Strains Mib1 MISSION shRNA Millipore Sigma Cat: SHCLNG-NM_144860 Mib1 shRNA Dharmacon Clones: V2LHS_35581 and V2LHS_35584 FUGW-NFIA-GFP B. Deneen, Baylor College of Medicine N/A NFIA tm1a (EUCOMM) Hmgu Mouse Genome Informatics MGI: 4458675 AAV-NEXT-V5 L. Alberi, Swiss Integrative Center for Human Health N/A pmaxGFP Lonza/Amaxa N/A Chemicals, Peptides, and Recombinant Proteins Tamoxifen Sigma Cat: T5648 EGF Sigma Cat: E2759-5X15MG FGF1 VWR International Cat: 89508-958 FGF10 R&D Systems Cat: 345-FG Heparin Sigma Cat: H3149-10KU R-Spondin1 R&D Systems Cat: 4645-RS-025 Y-27632 R&D Systems Cat: 1254-10 PMA Sigma Cat: P1585 CHIR-99021 Stemgent Cat: 04-0004-10 rhActivinA Peprotech Cat: 120-14E KGF Peprotech Cat: 100-19 RA Sigma Cat: R2625 SANT-1 Sigma Cat: S4572 rhNoggin Peprotech Cat: 120-10C Critical Commercial Assays TruSeq Stranded mRNA kit Illumina Cat: 20020594 Deposited Data Raw data files from RNA-seq This paper GEO: GSE86909 Experimental Models: Cell Lines H1 human ES cell line (WA01) WiCell Research Institute, Inc. RRID:CVCL_9771 Experimental Models: Organisms/Strains Mouse: Pdx1-cre Jackson Laboratory Stock: 024968 Mouse: Ptf1a-cre Jackson Laboratory Stock: 023329 Mouse: Sox9-creERt2 Jackson Laboratory Stock: 018829 Mouse: NFIA-KO R. Gronostajski, University of Buffalo Jacobs School of Medicine and Biomedical Sciences N/A Mouse: Ngn3-eGFP Mouse Genome Informatics MGI: 2651394 Mouse: CBF1:H2B-Venus Jackson Laboratory Stock: 020942 Oligonucleotides Primers for qPCR, ChIP-qPCR and genotyping PCR This paper see Table S1 Software and Algorithms ImageJ NIH https://imagej.nih.gov/ij/; RRID: SCR_003070 Adobe Photoshop CS5.1 Adobe RRID: SCR_014199 Microsoft Excel 2011 Microsoft RRID: SCR_016137 Prism 5 Graphpad RRID: SCR_002798 (Continued on next page) Cell Reports 25, 3811–3827.e1–e7, December 26, 2018 e2 ..

    RNA Sequencing:

    Article Title: Pancreatic Cell Fate Determination Relies on Notch Ligand Trafficking by NFIA.
    Article Snippet: .. Cat: 127-025-160; RRID: AB_2338974 Bacterial and Virus Strains Mib1 MISSION shRNA Millipore Sigma Cat: SHCLNG-NM_144860 Mib1 shRNA Dharmacon Clones: V2LHS_35581 and V2LHS_35584 FUGW-NFIA-GFP B. Deneen, Baylor College of Medicine N/A NFIA tm1a (EUCOMM) Hmgu Mouse Genome Informatics MGI: 4458675 AAV-NEXT-V5 L. Alberi, Swiss Integrative Center for Human Health N/A pmaxGFP Lonza/Amaxa N/A Chemicals, Peptides, and Recombinant Proteins Tamoxifen Sigma Cat: T5648 EGF Sigma Cat: E2759-5X15MG FGF1 VWR International Cat: 89508-958 FGF10 R&D Systems Cat: 345-FG Heparin Sigma Cat: H3149-10KU R-Spondin1 R&D Systems Cat: 4645-RS-025 Y-27632 R&D Systems Cat: 1254-10 PMA Sigma Cat: P1585 CHIR-99021 Stemgent Cat: 04-0004-10 rhActivinA Peprotech Cat: 120-14E KGF Peprotech Cat: 100-19 RA Sigma Cat: R2625 SANT-1 Sigma Cat: S4572 rhNoggin Peprotech Cat: 120-10C Critical Commercial Assays TruSeq Stranded mRNA kit Illumina Cat: 20020594 Deposited Data Raw data files from RNA-seq This paper GEO: GSE86909 Experimental Models: Cell Lines H1 human ES cell line (WA01) WiCell Research Institute, Inc. RRID:CVCL_9771 Experimental Models: Organisms/Strains Mouse: Pdx1-cre Jackson Laboratory Stock: 024968 Mouse: Ptf1a-cre Jackson Laboratory Stock: 023329 Mouse: Sox9-creERt2 Jackson Laboratory Stock: 018829 Mouse: NFIA-KO R. Gronostajski, University of Buffalo Jacobs School of Medicine and Biomedical Sciences N/A Mouse: Ngn3-eGFP Mouse Genome Informatics MGI: 2651394 Mouse: CBF1:H2B-Venus Jackson Laboratory Stock: 020942 Oligonucleotides Primers for qPCR, ChIP-qPCR and genotyping PCR This paper see Table S1 Software and Algorithms ImageJ NIH https://imagej.nih.gov/ij/; RRID: SCR_003070 Adobe Photoshop CS5.1 Adobe RRID: SCR_014199 Microsoft Excel 2011 Microsoft RRID: SCR_016137 Prism 5 Graphpad RRID: SCR_002798 (Continued on next page) Cell Reports 25, 3811–3827.e1–e7, December 26, 2018 e2 ..

    Real-time Polymerase Chain Reaction:

    Article Title: Pancreatic Cell Fate Determination Relies on Notch Ligand Trafficking by NFIA.
    Article Snippet: .. Cat: 127-025-160; RRID: AB_2338974 Bacterial and Virus Strains Mib1 MISSION shRNA Millipore Sigma Cat: SHCLNG-NM_144860 Mib1 shRNA Dharmacon Clones: V2LHS_35581 and V2LHS_35584 FUGW-NFIA-GFP B. Deneen, Baylor College of Medicine N/A NFIA tm1a (EUCOMM) Hmgu Mouse Genome Informatics MGI: 4458675 AAV-NEXT-V5 L. Alberi, Swiss Integrative Center for Human Health N/A pmaxGFP Lonza/Amaxa N/A Chemicals, Peptides, and Recombinant Proteins Tamoxifen Sigma Cat: T5648 EGF Sigma Cat: E2759-5X15MG FGF1 VWR International Cat: 89508-958 FGF10 R&D Systems Cat: 345-FG Heparin Sigma Cat: H3149-10KU R-Spondin1 R&D Systems Cat: 4645-RS-025 Y-27632 R&D Systems Cat: 1254-10 PMA Sigma Cat: P1585 CHIR-99021 Stemgent Cat: 04-0004-10 rhActivinA Peprotech Cat: 120-14E KGF Peprotech Cat: 100-19 RA Sigma Cat: R2625 SANT-1 Sigma Cat: S4572 rhNoggin Peprotech Cat: 120-10C Critical Commercial Assays TruSeq Stranded mRNA kit Illumina Cat: 20020594 Deposited Data Raw data files from RNA-seq This paper GEO: GSE86909 Experimental Models: Cell Lines H1 human ES cell line (WA01) WiCell Research Institute, Inc. RRID:CVCL_9771 Experimental Models: Organisms/Strains Mouse: Pdx1-cre Jackson Laboratory Stock: 024968 Mouse: Ptf1a-cre Jackson Laboratory Stock: 023329 Mouse: Sox9-creERt2 Jackson Laboratory Stock: 018829 Mouse: NFIA-KO R. Gronostajski, University of Buffalo Jacobs School of Medicine and Biomedical Sciences N/A Mouse: Ngn3-eGFP Mouse Genome Informatics MGI: 2651394 Mouse: CBF1:H2B-Venus Jackson Laboratory Stock: 020942 Oligonucleotides Primers for qPCR, ChIP-qPCR and genotyping PCR This paper see Table S1 Software and Algorithms ImageJ NIH https://imagej.nih.gov/ij/; RRID: SCR_003070 Adobe Photoshop CS5.1 Adobe RRID: SCR_014199 Microsoft Excel 2011 Microsoft RRID: SCR_016137 Prism 5 Graphpad RRID: SCR_002798 (Continued on next page) Cell Reports 25, 3811–3827.e1–e7, December 26, 2018 e2 ..

    ChIP-qPCR:

    Article Title: Pancreatic Cell Fate Determination Relies on Notch Ligand Trafficking by NFIA.
    Article Snippet: .. Cat: 127-025-160; RRID: AB_2338974 Bacterial and Virus Strains Mib1 MISSION shRNA Millipore Sigma Cat: SHCLNG-NM_144860 Mib1 shRNA Dharmacon Clones: V2LHS_35581 and V2LHS_35584 FUGW-NFIA-GFP B. Deneen, Baylor College of Medicine N/A NFIA tm1a (EUCOMM) Hmgu Mouse Genome Informatics MGI: 4458675 AAV-NEXT-V5 L. Alberi, Swiss Integrative Center for Human Health N/A pmaxGFP Lonza/Amaxa N/A Chemicals, Peptides, and Recombinant Proteins Tamoxifen Sigma Cat: T5648 EGF Sigma Cat: E2759-5X15MG FGF1 VWR International Cat: 89508-958 FGF10 R&D Systems Cat: 345-FG Heparin Sigma Cat: H3149-10KU R-Spondin1 R&D Systems Cat: 4645-RS-025 Y-27632 R&D Systems Cat: 1254-10 PMA Sigma Cat: P1585 CHIR-99021 Stemgent Cat: 04-0004-10 rhActivinA Peprotech Cat: 120-14E KGF Peprotech Cat: 100-19 RA Sigma Cat: R2625 SANT-1 Sigma Cat: S4572 rhNoggin Peprotech Cat: 120-10C Critical Commercial Assays TruSeq Stranded mRNA kit Illumina Cat: 20020594 Deposited Data Raw data files from RNA-seq This paper GEO: GSE86909 Experimental Models: Cell Lines H1 human ES cell line (WA01) WiCell Research Institute, Inc. RRID:CVCL_9771 Experimental Models: Organisms/Strains Mouse: Pdx1-cre Jackson Laboratory Stock: 024968 Mouse: Ptf1a-cre Jackson Laboratory Stock: 023329 Mouse: Sox9-creERt2 Jackson Laboratory Stock: 018829 Mouse: NFIA-KO R. Gronostajski, University of Buffalo Jacobs School of Medicine and Biomedical Sciences N/A Mouse: Ngn3-eGFP Mouse Genome Informatics MGI: 2651394 Mouse: CBF1:H2B-Venus Jackson Laboratory Stock: 020942 Oligonucleotides Primers for qPCR, ChIP-qPCR and genotyping PCR This paper see Table S1 Software and Algorithms ImageJ NIH https://imagej.nih.gov/ij/; RRID: SCR_003070 Adobe Photoshop CS5.1 Adobe RRID: SCR_014199 Microsoft Excel 2011 Microsoft RRID: SCR_016137 Prism 5 Graphpad RRID: SCR_002798 (Continued on next page) Cell Reports 25, 3811–3827.e1–e7, December 26, 2018 e2 ..

    Polymerase Chain Reaction:

    Article Title: Pancreatic Cell Fate Determination Relies on Notch Ligand Trafficking by NFIA.
    Article Snippet: .. Cat: 127-025-160; RRID: AB_2338974 Bacterial and Virus Strains Mib1 MISSION shRNA Millipore Sigma Cat: SHCLNG-NM_144860 Mib1 shRNA Dharmacon Clones: V2LHS_35581 and V2LHS_35584 FUGW-NFIA-GFP B. Deneen, Baylor College of Medicine N/A NFIA tm1a (EUCOMM) Hmgu Mouse Genome Informatics MGI: 4458675 AAV-NEXT-V5 L. Alberi, Swiss Integrative Center for Human Health N/A pmaxGFP Lonza/Amaxa N/A Chemicals, Peptides, and Recombinant Proteins Tamoxifen Sigma Cat: T5648 EGF Sigma Cat: E2759-5X15MG FGF1 VWR International Cat: 89508-958 FGF10 R&D Systems Cat: 345-FG Heparin Sigma Cat: H3149-10KU R-Spondin1 R&D Systems Cat: 4645-RS-025 Y-27632 R&D Systems Cat: 1254-10 PMA Sigma Cat: P1585 CHIR-99021 Stemgent Cat: 04-0004-10 rhActivinA Peprotech Cat: 120-14E KGF Peprotech Cat: 100-19 RA Sigma Cat: R2625 SANT-1 Sigma Cat: S4572 rhNoggin Peprotech Cat: 120-10C Critical Commercial Assays TruSeq Stranded mRNA kit Illumina Cat: 20020594 Deposited Data Raw data files from RNA-seq This paper GEO: GSE86909 Experimental Models: Cell Lines H1 human ES cell line (WA01) WiCell Research Institute, Inc. RRID:CVCL_9771 Experimental Models: Organisms/Strains Mouse: Pdx1-cre Jackson Laboratory Stock: 024968 Mouse: Ptf1a-cre Jackson Laboratory Stock: 023329 Mouse: Sox9-creERt2 Jackson Laboratory Stock: 018829 Mouse: NFIA-KO R. Gronostajski, University of Buffalo Jacobs School of Medicine and Biomedical Sciences N/A Mouse: Ngn3-eGFP Mouse Genome Informatics MGI: 2651394 Mouse: CBF1:H2B-Venus Jackson Laboratory Stock: 020942 Oligonucleotides Primers for qPCR, ChIP-qPCR and genotyping PCR This paper see Table S1 Software and Algorithms ImageJ NIH https://imagej.nih.gov/ij/; RRID: SCR_003070 Adobe Photoshop CS5.1 Adobe RRID: SCR_014199 Microsoft Excel 2011 Microsoft RRID: SCR_016137 Prism 5 Graphpad RRID: SCR_002798 (Continued on next page) Cell Reports 25, 3811–3827.e1–e7, December 26, 2018 e2 ..

    Software:

    Article Title: Pancreatic Cell Fate Determination Relies on Notch Ligand Trafficking by NFIA.
    Article Snippet: .. Cat: 127-025-160; RRID: AB_2338974 Bacterial and Virus Strains Mib1 MISSION shRNA Millipore Sigma Cat: SHCLNG-NM_144860 Mib1 shRNA Dharmacon Clones: V2LHS_35581 and V2LHS_35584 FUGW-NFIA-GFP B. Deneen, Baylor College of Medicine N/A NFIA tm1a (EUCOMM) Hmgu Mouse Genome Informatics MGI: 4458675 AAV-NEXT-V5 L. Alberi, Swiss Integrative Center for Human Health N/A pmaxGFP Lonza/Amaxa N/A Chemicals, Peptides, and Recombinant Proteins Tamoxifen Sigma Cat: T5648 EGF Sigma Cat: E2759-5X15MG FGF1 VWR International Cat: 89508-958 FGF10 R&D Systems Cat: 345-FG Heparin Sigma Cat: H3149-10KU R-Spondin1 R&D Systems Cat: 4645-RS-025 Y-27632 R&D Systems Cat: 1254-10 PMA Sigma Cat: P1585 CHIR-99021 Stemgent Cat: 04-0004-10 rhActivinA Peprotech Cat: 120-14E KGF Peprotech Cat: 100-19 RA Sigma Cat: R2625 SANT-1 Sigma Cat: S4572 rhNoggin Peprotech Cat: 120-10C Critical Commercial Assays TruSeq Stranded mRNA kit Illumina Cat: 20020594 Deposited Data Raw data files from RNA-seq This paper GEO: GSE86909 Experimental Models: Cell Lines H1 human ES cell line (WA01) WiCell Research Institute, Inc. RRID:CVCL_9771 Experimental Models: Organisms/Strains Mouse: Pdx1-cre Jackson Laboratory Stock: 024968 Mouse: Ptf1a-cre Jackson Laboratory Stock: 023329 Mouse: Sox9-creERt2 Jackson Laboratory Stock: 018829 Mouse: NFIA-KO R. Gronostajski, University of Buffalo Jacobs School of Medicine and Biomedical Sciences N/A Mouse: Ngn3-eGFP Mouse Genome Informatics MGI: 2651394 Mouse: CBF1:H2B-Venus Jackson Laboratory Stock: 020942 Oligonucleotides Primers for qPCR, ChIP-qPCR and genotyping PCR This paper see Table S1 Software and Algorithms ImageJ NIH https://imagej.nih.gov/ij/; RRID: SCR_003070 Adobe Photoshop CS5.1 Adobe RRID: SCR_014199 Microsoft Excel 2011 Microsoft RRID: SCR_016137 Prism 5 Graphpad RRID: SCR_002798 (Continued on next page) Cell Reports 25, 3811–3827.e1–e7, December 26, 2018 e2 ..



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