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PeproTech rhfgf-b
Rhfgf B, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rhfgf-b/hfgf+b/pmc10694756__mmc2-418-71-72
Average 90 stars, based on 1 article reviews
rhfgf-b - by Bioz Stars, 2026-10
90/100 stars

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Article Title: Protocol for live-cell imaging of immune synapse formation and activation of CAR T cells against cancer cells.
Article Snippet: Data analysis by using Fiji, a freely available software for image analysis Immune synapse (IS) formation determines T cell antitumor activity.. Here, we present a protocol for characterizing the IS formation between chimeric antigen receptor (CAR) T cells and tumor cells by measuring the IS size and calcium flux by live-cell imaging.. We describe steps for CAR T cell manufacturing, sample preparation, image acquisition, and data analysis.

Article Title: GRP78-CAR T cell effector function against solid and brain tumors is controlled by GRP78 expression on T cells
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies CD3 Miltenyi Biotec Cat#130-093-337 CD28 Miltenyi Biotec Cat#130-093-386 CD19-APC BD Biosciences Cat#555415 CD8-PerCP Biolegend Cat#344708 CD45RA-APC Biolegend Cat#304112 CCR7-FITC Biolegend Cat#353216 CD4-PE/Cy7 Biolegend Cat#344612 PD1-PE Biolegend Cat#329905 TIM3-PE/Cy7 Biolegend Cat#345013 LAG3-FITC Biolegend Cat#369307 HSPA5 Atlas antibodies Cat#HPA038845 Isotype Agilent DAKO Cat#X090302 Biological samples Human peripheral blood mononuclear cells (PBMCs) Deidentified healthy donors St. Jude Chemicals, peptides, and recombinant proteins rhIL-7 Peprotech Cat#200-7 rhIL-15 Peprotech Cat#200-15 rhFGF-b Peprotech Cat#100-18B rhEGF Peprotech Cat#AF-100-15 B27 Thermofisher Cat#12587010 N2 Thermofisher Cat#17502048 GeneJuice Novagen Cat#70967 Heparin Stemcell technologies Cat#7980 Lymphoprep Abbott Laboratories Cat#07811 Retronectin Clontech Cat#T100B Cas9 MacroLab N/A P3 Primary cell buffer Lonza Cat#V4XP-3032 Dasatinib LC Labs Cat#D-3307 LIVE/DEAD Aqua Fisher Scientific Cat#L34957 Celltracker Red CMTPX Invitrogen Cat#C34552 CAL520a.m.

Article Title: Secreted factors from synovial fibroblasts immediately regulate gene expression in articular chondrocytes.
Article Snippet: Accepted Manuscript Secreted factors from synovial fibroblasts immediately regulate gene expression in articular chondrocytes Martin Bonitz, Christiane Schaffer, Michael Amling, Ralf Poertner, Thorsten Schinke, Anke Jeschke PII: S0378-1119(19)30199-4 DOI: https://doi.org/10.1016/j.gene.2019.02.065 Reference: GENE 43646 To appear in: Gene Received date: 21 February 2019 Accepted date: 21 February 2019 Please cite this article as: M. Bonitz, C. Schaffer, M. Amling, et al., Secreted factors from synovial fibroblasts immediately regulate gene expression in articular chondrocytes, Gene, https://doi.org/10.1016/j.gene.2019.02.065 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

Recombinant:

Article Title: Protocol for live-cell imaging of immune synapse formation and activation of CAR T cells against cancer cells.
Article Snippet: Data analysis by using Fiji, a freely available software for image analysis Immune synapse (IS) formation determines T cell antitumor activity.. Here, we present a protocol for characterizing the IS formation between chimeric antigen receptor (CAR) T cells and tumor cells by measuring the IS size and calcium flux by live-cell imaging.. We describe steps for CAR T cell manufacturing, sample preparation, image acquisition, and data analysis.

Article Title: GRP78-CAR T cell effector function against solid and brain tumors is controlled by GRP78 expression on T cells
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies CD3 Miltenyi Biotec Cat#130-093-337 CD28 Miltenyi Biotec Cat#130-093-386 CD19-APC BD Biosciences Cat#555415 CD8-PerCP Biolegend Cat#344708 CD45RA-APC Biolegend Cat#304112 CCR7-FITC Biolegend Cat#353216 CD4-PE/Cy7 Biolegend Cat#344612 PD1-PE Biolegend Cat#329905 TIM3-PE/Cy7 Biolegend Cat#345013 LAG3-FITC Biolegend Cat#369307 HSPA5 Atlas antibodies Cat#HPA038845 Isotype Agilent DAKO Cat#X090302 Biological samples Human peripheral blood mononuclear cells (PBMCs) Deidentified healthy donors St. Jude Chemicals, peptides, and recombinant proteins rhIL-7 Peprotech Cat#200-7 rhIL-15 Peprotech Cat#200-15 rhFGF-b Peprotech Cat#100-18B rhEGF Peprotech Cat#AF-100-15 B27 Thermofisher Cat#12587010 N2 Thermofisher Cat#17502048 GeneJuice Novagen Cat#70967 Heparin Stemcell technologies Cat#7980 Lymphoprep Abbott Laboratories Cat#07811 Retronectin Clontech Cat#T100B Cas9 MacroLab N/A P3 Primary cell buffer Lonza Cat#V4XP-3032 Dasatinib LC Labs Cat#D-3307 LIVE/DEAD Aqua Fisher Scientific Cat#L34957 Celltracker Red CMTPX Invitrogen Cat#C34552 CAL520a.m.

Article Title: Secreted factors from synovial fibroblasts immediately regulate gene expression in articular chondrocytes.
Article Snippet: Accepted Manuscript Secreted factors from synovial fibroblasts immediately regulate gene expression in articular chondrocytes Martin Bonitz, Christiane Schaffer, Michael Amling, Ralf Poertner, Thorsten Schinke, Anke Jeschke PII: S0378-1119(19)30199-4 DOI: https://doi.org/10.1016/j.gene.2019.02.065 Reference: GENE 43646 To appear in: Gene Received date: 21 February 2019 Accepted date: 21 February 2019 Please cite this article as: M. Bonitz, C. Schaffer, M. Amling, et al., Secreted factors from synovial fibroblasts immediately regulate gene expression in articular chondrocytes, Gene, https://doi.org/10.1016/j.gene.2019.02.065 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

Software:

Article Title: Protocol for live-cell imaging of immune synapse formation and activation of CAR T cells against cancer cells.
Article Snippet: Data analysis by using Fiji, a freely available software for image analysis Immune synapse (IS) formation determines T cell antitumor activity.. Here, we present a protocol for characterizing the IS formation between chimeric antigen receptor (CAR) T cells and tumor cells by measuring the IS size and calcium flux by live-cell imaging.. We describe steps for CAR T cell manufacturing, sample preparation, image acquisition, and data analysis.

Article Title: GRP78-CAR T cell effector function against solid and brain tumors is controlled by GRP78 expression on T cells
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies CD3 Miltenyi Biotec Cat#130-093-337 CD28 Miltenyi Biotec Cat#130-093-386 CD19-APC BD Biosciences Cat#555415 CD8-PerCP Biolegend Cat#344708 CD45RA-APC Biolegend Cat#304112 CCR7-FITC Biolegend Cat#353216 CD4-PE/Cy7 Biolegend Cat#344612 PD1-PE Biolegend Cat#329905 TIM3-PE/Cy7 Biolegend Cat#345013 LAG3-FITC Biolegend Cat#369307 HSPA5 Atlas antibodies Cat#HPA038845 Isotype Agilent DAKO Cat#X090302 Biological samples Human peripheral blood mononuclear cells (PBMCs) Deidentified healthy donors St. Jude Chemicals, peptides, and recombinant proteins rhIL-7 Peprotech Cat#200-7 rhIL-15 Peprotech Cat#200-15 rhFGF-b Peprotech Cat#100-18B rhEGF Peprotech Cat#AF-100-15 B27 Thermofisher Cat#12587010 N2 Thermofisher Cat#17502048 GeneJuice Novagen Cat#70967 Heparin Stemcell technologies Cat#7980 Lymphoprep Abbott Laboratories Cat#07811 Retronectin Clontech Cat#T100B Cas9 MacroLab N/A P3 Primary cell buffer Lonza Cat#V4XP-3032 Dasatinib LC Labs Cat#D-3307 LIVE/DEAD Aqua Fisher Scientific Cat#L34957 Celltracker Red CMTPX Invitrogen Cat#C34552 CAL520a.m.

Article Title: Secreted factors from synovial fibroblasts immediately regulate gene expression in articular chondrocytes.
Article Snippet: Accepted Manuscript Secreted factors from synovial fibroblasts immediately regulate gene expression in articular chondrocytes Martin Bonitz, Christiane Schaffer, Michael Amling, Ralf Poertner, Thorsten Schinke, Anke Jeschke PII: S0378-1119(19)30199-4 DOI: https://doi.org/10.1016/j.gene.2019.02.065 Reference: GENE 43646 To appear in: Gene Received date: 21 February 2019 Accepted date: 21 February 2019 Please cite this article as: M. Bonitz, C. Schaffer, M. Amling, et al., Secreted factors from synovial fibroblasts immediately regulate gene expression in articular chondrocytes, Gene, https://doi.org/10.1016/j.gene.2019.02.065 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

Sterility:

Article Title: Protocol for live-cell imaging of immune synapse formation and activation of CAR T cells against cancer cells.
Article Snippet: Data analysis by using Fiji, a freely available software for image analysis Immune synapse (IS) formation determines T cell antitumor activity.. Here, we present a protocol for characterizing the IS formation between chimeric antigen receptor (CAR) T cells and tumor cells by measuring the IS size and calcium flux by live-cell imaging.. We describe steps for CAR T cell manufacturing, sample preparation, image acquisition, and data analysis.

Article Title: GRP78-CAR T cell effector function against solid and brain tumors is controlled by GRP78 expression on T cells
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies CD3 Miltenyi Biotec Cat#130-093-337 CD28 Miltenyi Biotec Cat#130-093-386 CD19-APC BD Biosciences Cat#555415 CD8-PerCP Biolegend Cat#344708 CD45RA-APC Biolegend Cat#304112 CCR7-FITC Biolegend Cat#353216 CD4-PE/Cy7 Biolegend Cat#344612 PD1-PE Biolegend Cat#329905 TIM3-PE/Cy7 Biolegend Cat#345013 LAG3-FITC Biolegend Cat#369307 HSPA5 Atlas antibodies Cat#HPA038845 Isotype Agilent DAKO Cat#X090302 Biological samples Human peripheral blood mononuclear cells (PBMCs) Deidentified healthy donors St. Jude Chemicals, peptides, and recombinant proteins rhIL-7 Peprotech Cat#200-7 rhIL-15 Peprotech Cat#200-15 rhFGF-b Peprotech Cat#100-18B rhEGF Peprotech Cat#AF-100-15 B27 Thermofisher Cat#12587010 N2 Thermofisher Cat#17502048 GeneJuice Novagen Cat#70967 Heparin Stemcell technologies Cat#7980 Lymphoprep Abbott Laboratories Cat#07811 Retronectin Clontech Cat#T100B Cas9 MacroLab N/A P3 Primary cell buffer Lonza Cat#V4XP-3032 Dasatinib LC Labs Cat#D-3307 LIVE/DEAD Aqua Fisher Scientific Cat#L34957 Celltracker Red CMTPX Invitrogen Cat#C34552 CAL520a.m.

Article Title: Secreted factors from synovial fibroblasts immediately regulate gene expression in articular chondrocytes.
Article Snippet: Accepted Manuscript Secreted factors from synovial fibroblasts immediately regulate gene expression in articular chondrocytes Martin Bonitz, Christiane Schaffer, Michael Amling, Ralf Poertner, Thorsten Schinke, Anke Jeschke PII: S0378-1119(19)30199-4 DOI: https://doi.org/10.1016/j.gene.2019.02.065 Reference: GENE 43646 To appear in: Gene Received date: 21 February 2019 Accepted date: 21 February 2019 Please cite this article as: M. Bonitz, C. Schaffer, M. Amling, et al., Secreted factors from synovial fibroblasts immediately regulate gene expression in articular chondrocytes, Gene, https://doi.org/10.1016/j.gene.2019.02.065 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.



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