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random primer (TaKaRa)

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TaKaRa random primer
Random Primer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 6526 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/random/Random+Primer/pmc13652265-197-0-9
Average 96 stars, based on 6526 article reviews

random primer - by Bioz Stars, 2026-11

96/100 stars

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Mutagenesis:

Article Title: Allosteric disulfide control of ligand binding and endocytosis of KIR2DL4, the natural killer cell receptor for HLA-G
Article Snippet: .. Random mutagenesis of the KIR2DL4 sequence spanning amino acids 1–208, consisting of the Ig domains D0 and D2 and a portion of the stem was carried out using the Diversify PCR Random Mutagenesis kit (Clontech, Cat630703) according to the manufacturer’s instructions. ..

Sequencing:

Polymerase Chain Reaction:

cDNA Synthesis:

Article Title: Telomere maintenance mechanisms are activated in ganglioneuroblastoma and ganglioneuroma
Article Snippet: .. Total RNA from human frozen tissue was extracted by ISOGEN II (NIPPON GENE) and cDNA synthesis was performed by ReverTra Ace ® (TOYOBO) and random primers (Takara Bio) according to the manufacturer's instructions. .. RT-qPCR analysis was conducted in triplicate using QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems).

Article Title: CircPRKCA Promotes NSCLC Progression via miR-200b-3p/FRMD6/SNAI2 Axis.
Article Snippet: .. To validate circRNA backspliced junctions, total RNA was treated with the RiboZero rRNA Removal Kit (Epicentre, Madison, WI, USA) to deplete rRNA (per the manufacturer’s instructions), followed by cDNA synthesis from rRNA-depleted and RNase R-digested RNA using random primers (Takara, Dalian, China). .. For mRNA and circRNA quantification, cDNA was synthesized from 500 ng RNA with the PrimeScript RT Master Mix (Takara).

Reverse Transcription:

Article Title: Cucumber Mosaic Virus Coat Protein Sequesters Host CDPK7 ‐Like Into Phase‐Separated Condensates to Promote Viral Infection
Article Snippet: The leaves of the assayed N. benthamiana were used to extract total RNA by RNAiso Plus (TaKaRa). .. Reverse transcription was carried out using gene‐specific primers or random primers and a reverse transcriptase kit (TaKaRa) according to the instructions. .. The 2× Hieff PCR Master Mix (Yeasen) was used to execute PCR. qPCR was conducted using the TB Green Premix Ex Taq II (TaKaRa).

Reverse Transcription Polymerase Chain Reaction:

Article Title: piR-823 and piR-20326 Differentially Regulate Stemness Programs in Ovarian Cancer Stem-like Cells.
Article Snippet: .. Diluted RNAs (50ng/ul) and random primers were used according to the “PrimeScript RT Master Mix Synthesis System for RT-PCR (Takara Bio USA, Inc., San Jose, CA, USA)” (Table S3). .. “SsoAdvanced Universal SYBR Green Supermix (Bio-Rad Laboratories, Hercules, CA, USA)” master mix was used for mRNA expression analysis of genes.