10953 δ radd strains  (ATCC)

Journal: bioRxiv

Article Title: RadD from Fusobacterium nucleatum Engages NKp46 to Promote Antitumor Cytotoxicity

doi: 10.1101/2025.07.26.666929

Figure Lengend Snippet: The figure shows histograms of FITC-labeled F. nucleatum subsp. nucleatum ATCC 23726 (FN23726) (upper histograms) and ATCC 10953 (lower histograms) incubated with 2 μg of NKp46 Ig, D1 domain of NKp46 (D1 Ig), Ncr-1 Ig, and CD16 Ig fusion proteins. Representative staining from one of two independent experiments is shown.

Article Snippet: Intriguingly, infection with either the wild-type ATCC 10953 or ATCC 10953 Δ RadD strains was not able to affect tumor progression in NCR-1 KO mice.

Techniques: Labeling, Incubation, Staining

Journal: bioRxiv

Article Title: RadD from Fusobacterium nucleatum Engages NKp46 to Promote Antitumor Cytotoxicity

doi: 10.1101/2025.07.26.666929

Figure Lengend Snippet: A-B. Density plot of FITC labeled ATCC 10953 (A) and its ΔfadI mutant derivative ATCC 10953 ΔFad-I (B) stained with the various fusion proteins (listed in the X axis). C. Schematic representation of ATCC 10953 wild type (WT) strain and RadD surface expression (Left) compared to ATCC 10953 ΔFad-I (Right). D. Density plot of the FITC-labeled ΔRadD mutant strain of ATCC 10953 stained with various fusion proteins (listed in the X axis). The figure shows data from one representative experiment out of three to five independent experiments. E. Fold change quantification of FITC-labeled bacteria binding to the fusion proteins Ccm1-Ig, NKp46 Ig and Ncr-1 Ig in ATCC 10953 (left) and ATCC 23726 (right). Summary of three to five independent experiments. The mean value ±SD of the experiments is presented. * P < 0.05, ** P ≤ 0.01, *** P ≤ 0.001 and **** P ≤ 0.0001.

Article Snippet: Intriguingly, infection with either the wild-type ATCC 10953 or ATCC 10953 Δ RadD strains was not able to affect tumor progression in NCR-1 KO mice.

Techniques: Labeling, Mutagenesis, Staining, Expressing, Bacteria, Binding Assay

Journal: bioRxiv

Article Title: RadD from Fusobacterium nucleatum Engages NKp46 to Promote Antitumor Cytotoxicity

doi: 10.1101/2025.07.26.666929

Figure Lengend Snippet: A. Quantification of Median Fluorescent Intensity (MFI) of FITC-labeled ATCC 10953 binding to Ccm1-Ig, NKp46-Ig, and Ncr-1 Ig, without or with 5 and 10 mM of L-Arginine. Data combined from three to four independent experiments are presented. B. NKp46-Ig (2 µg) was pre-incubated with 1 µg of a control anti-PVR antibody and NKp46 monoclonal antibodies (9E2, 461-G1, and 02) to evaluate the blocking of ATCC 10953 interaction with the NKp46 receptor. C. Shows the quantification results of histograms depicted in A. The mean value ±SD of the experiments is presented. * P < 0.05, ** P ≤ 0.01, *** P ≤ 0.001 and **** P ≤ 0.0001.

Article Snippet: Intriguingly, infection with either the wild-type ATCC 10953 or ATCC 10953 Δ RadD strains was not able to affect tumor progression in NCR-1 KO mice.

Techniques: Labeling, Binding Assay, Incubation, Control, Bioprocessing, Blocking Assay

Journal: bioRxiv

Article Title: RadD from Fusobacterium nucleatum Engages NKp46 to Promote Antitumor Cytotoxicity

doi: 10.1101/2025.07.26.666929

Figure Lengend Snippet: A. Schematic diagram showing the design of the NK cells cytotoxicity assay against breast cancer cell lines T47D and MCF7. 1. Tumor cells were stained with Calcein-AM dye and then incubated either with tumor cells (T47D or MCF7) only, tumor + NK, tumor + bacteria ( ATCC 10953 WT and ATCC 10953 ΔRadD ) + NK with/without preincubation with 02 antibody. 2. Killing assays were performed in a 37°C incubator for 4 hours. 3. The fluorescence intensity of Calcein was measured to determine cell viability using a spectrophotometer (Tecan Spark). Summary of NK cytotoxicity against T47D (B) and MCF7 (C) breast cancer cell lines. Combined results from five independent experiments. D. C57BL\6 or NCR1-KO mice were shaved and AT3 cells (1 x 10 ^6 cells in 100 μl PBS) were injected one day later into the mammary fat pad. When tumors reached a size of about 500 mm 3 , mice were inoculated intravenously with 5 × 10 7 ATCC 10953 WT and 7.5 × 10 7 ATCC 10953 Δ RadD bacteria. Eight days later, mice were sacrificed and tumor weight was determined. E. The tumor weight of C57BL or NCR1-KO (F) mice. The figure shows the combination of 4-5 experiments performed. The mean value ±SD of the experiments is presented. * P < 0.05, ** P ≤ 0.01, *** P ≤ 0.001 and **** P ≤ 0.0001.

Article Snippet: Intriguingly, infection with either the wild-type ATCC 10953 or ATCC 10953 Δ RadD strains was not able to affect tumor progression in NCR-1 KO mice.

Techniques: Cytotoxicity Assay, Staining, Incubation, Bacteria, Fluorescence, Spectrophotometry, Injection

Journal: bioRxiv

Article Title: RadD from Fusobacterium nucleatum Engages NKp46 to Promote Antitumor Cytotoxicity

doi: 10.1101/2025.07.26.666929

Figure Lengend Snippet: A-B. Density plot of FITC labeled ATCC 10953 (A) and its ΔfadI mutant derivative ATCC 10953 ΔFad-I (B) stained with the various fusion proteins (listed in the X axis). C. Schematic representation of ATCC 10953 wild type (WT) strain and RadD surface expression (Left) compared to ATCC 10953 ΔFad-I (Right). D. Density plot of the FITC-labeled ΔRadD mutant strain of ATCC 10953 stained with various fusion proteins (listed in the X axis). The figure shows data from one representative experiment out of three to five independent experiments. E. Fold change quantification of FITC-labeled bacteria binding to the fusion proteins Ccm1-Ig, NKp46 Ig and Ncr-1 Ig in ATCC 10953 (left) and ATCC 23726 (right). Summary of three to five independent experiments. The mean value ±SD of the experiments is presented. * P < 0.05, ** P ≤ 0.01, *** P ≤ 0.001 and **** P ≤ 0.0001.

Article Snippet: Since we showed previously that the absence of FadI results in overexpression of RadD ( and ( )), we incubated Ccm-1 Ig, NKp46 Ig and Ncr-1 Ig with a FITC-labeled ATCC 10953 mutant lacking the major multifunctional adhesin RadD ( ATCC 10593 ΔRadD ).

Techniques: Labeling, Mutagenesis, Staining, Expressing, Bacteria, Binding Assay

Journal: bioRxiv

Article Title: RadD from Fusobacterium nucleatum Engages NKp46 to Promote Antitumor Cytotoxicity

doi: 10.1101/2025.07.26.666929

Figure Lengend Snippet: A. Schematic diagram showing the design of the NK cells cytotoxicity assay against breast cancer cell lines T47D and MCF7. 1. Tumor cells were stained with Calcein-AM dye and then incubated either with tumor cells (T47D or MCF7) only, tumor + NK, tumor + bacteria ( ATCC 10953 WT and ATCC 10953 ΔRadD ) + NK with/without preincubation with 02 antibody. 2. Killing assays were performed in a 37°C incubator for 4 hours. 3. The fluorescence intensity of Calcein was measured to determine cell viability using a spectrophotometer (Tecan Spark). Summary of NK cytotoxicity against T47D (B) and MCF7 (C) breast cancer cell lines. Combined results from five independent experiments. D. C57BL\6 or NCR1-KO mice were shaved and AT3 cells (1 x 10 ^6 cells in 100 μl PBS) were injected one day later into the mammary fat pad. When tumors reached a size of about 500 mm 3 , mice were inoculated intravenously with 5 × 10 7 ATCC 10953 WT and 7.5 × 10 7 ATCC 10953 Δ RadD bacteria. Eight days later, mice were sacrificed and tumor weight was determined. E. The tumor weight of C57BL or NCR1-KO (F) mice. The figure shows the combination of 4-5 experiments performed. The mean value ±SD of the experiments is presented. * P < 0.05, ** P ≤ 0.01, *** P ≤ 0.001 and **** P ≤ 0.0001.

Article Snippet: Since we showed previously that the absence of FadI results in overexpression of RadD ( and ( )), we incubated Ccm-1 Ig, NKp46 Ig and Ncr-1 Ig with a FITC-labeled ATCC 10953 mutant lacking the major multifunctional adhesin RadD ( ATCC 10593 ΔRadD ).

Techniques: Cytotoxicity Assay, Staining, Incubation, Bacteria, Fluorescence, Spectrophotometry, Injection

Journal: bioRxiv

Article Title: RadD from Fusobacterium nucleatum Engages NKp46 to Promote Antitumor Cytotoxicity

doi: 10.1101/2025.07.26.666929

Figure Lengend Snippet: NKp46 interaction with RadD expressed by Fusobacterium nucleatum triggers NK cell cytotoxicity. This activation enhances tumor cell killing in vitro and in vivo . Conversely, the absence of RadD or the blocking of NKp46 impairs NK cell activity, leading to tumor progression. Created by BioRender.

Article Snippet: Since we showed previously that the absence of FadI results in overexpression of RadD ( and ( )), we incubated Ccm-1 Ig, NKp46 Ig and Ncr-1 Ig with a FITC-labeled ATCC 10953 mutant lacking the major multifunctional adhesin RadD ( ATCC 10593 ΔRadD ).

Techniques: Activation Assay, In Vitro, In Vivo, Blocking Assay, Activity Assay

Journal: bioRxiv

Article Title: Development of a Conditional Plasmid for Gene Deletion in Non-Model Fusobacterium nucleatum strains

doi: 10.1101/2024.09.09.612158

Figure Lengend Snippet: (A) Schematic of the pZP06B plasmid, in which the galK gene was replaced with sacB under the control of the rpsJ promoter, enabling sacB -based counterselection. (B) Schematic of pZP06C, an enhanced version of pZP06B, containing a mCherry reporter cassette in the multiple cloning site (MCS) to provide a visual marker for identifying positive clones. (C) Construction of pZP06CΔradD for deleting the radD gene in Fusobacterium nucleatum strain 21_1A. The radD gene encodes a large surface adhesion protein that facilitates coaggregation with oral bacteria. (D) PCR analysis using primer pair P7/P8 (indicated in panel C) of ten sucrose-resistant colonies after sacB counterselection in four Fusobacterium strains (21_1A, CTI-2, ATCC 10953, and ATCC 23726). In strain 21_1A, four colonies produced a 2.0 kb amplicon, indicating a radD deletion, while the remaining six colonies produced the 8.3 kb wild-type amplicon. In the other strains, the PCR results showed varying numbers of mutants and wild-type colonies, with smaller amplicons corresponding to the radD deletions and larger amplicons corresponding to the wild-type genotype. (E) Coaggregation assays with A. oris MG-1. Deletion of the radD gene abolished coaggregation in strains 21_1A, ATCC 10953, and ATCC 23726 but not in strain CTI-2. The wild-type and ΔradD mutant cells for each strain were cultured in TSPC medium to the stationary phase, then collected, washed, resuspended in coaggregation buffer, and assessed for coaggregation with an equal amount of A. oris cells. Representative results are shown from repeated experiments with all screened mutant colonies. (F) Western blot analysis of RadD expression in wild-type and ΔradD mutant strains. Cells used in the coaggregation assays in panel E underwent SDS-PAGE, followed by immunoblotting. Antibodies specific to RadD (α-RadD) and HsIJ (α-HsIJ) were used for detection, with HsIJ serving as a loading control . Molecular weight markers (in kilodaltons) are indicated on the left side of the blot.

Article Snippet: To improve specificity, we developed a new RadD antibody using a recombinant protein containing amino acids 44-200 of RadD from ATCC 23726, a region highly conserved among RadD homologs (Fig. S1).

Techniques: Plasmid Preparation, Control, Cloning, Marker, Clone Assay, Bacteria, Produced, Amplification, Mutagenesis, Cell Culture, Western Blot, Expressing, SDS Page, Molecular Weight

Journal: Parasitology Research

Article Title: Sex interacts with age-dependent change in the abundance of lice-infesting Amur Falcons ( Falco amurensis )

doi: 10.1007/s00436-020-06753-w

Figure Lengend Snippet: Descriptive statistics of the louse infestation of the Amur Falcons ( Falco amurensis ) by age, louse species and sex (SD: standard deviation)

Article Snippet: Amur Falcons ( Falco amurensis Radde, 1863) breed in East Asia (Transbaikalia, Amurland, North-Eastern China) and winter in southern Africa making their migratory route the longest among raptors.

Techniques: Infection

Journal: Parasitology Research

Article Title: Sex interacts with age-dependent change in the abundance of lice-infesting Amur Falcons ( Falco amurensis )

doi: 10.1007/s00436-020-06753-w

Figure Lengend Snippet: Abundances (and their 95% C.I.) of the different louse species on Amur Falcons ( Falco amurensis ) predicted by the GLMs

Article Snippet: Amur Falcons ( Falco amurensis Radde, 1863) breed in East Asia (Transbaikalia, Amurland, North-Eastern China) and winter in southern Africa making their migratory route the longest among raptors.

Techniques:

Journal: Parasitology Research

Article Title: Sex interacts with age-dependent change in the abundance of lice-infesting Amur Falcons ( Falco amurensis )

doi: 10.1007/s00436-020-06753-w

Figure Lengend Snippet: Results of the GLMs modelling the mean abundance of the louse species on the Amur Falcons ( Falco amurensis ). The mean abundance of Colpocephalum subzerafae is higher on juveniles than on males. In the case of Degeeriella rufa , we found interaction between the sex and the age of the birds. The mean abundance of D. rufa is similarly high among juveniles for both sexes, while it is higher for adult females than adult males

Article Snippet: Amur Falcons ( Falco amurensis Radde, 1863) breed in East Asia (Transbaikalia, Amurland, North-Eastern China) and winter in southern Africa making their migratory route the longest among raptors.

Techniques:

Journal: Microorganisms

Article Title: Role of FAD-I in Fusobacterial Interspecies Interaction and Biofilm Formation

doi: 10.3390/microorganisms8010070

Figure Lengend Snippet: List of qPCR primers used in the study.

Article Snippet: , Fnp_Δ radD , ATCC 10953::pBS24 , [ ] .

Techniques: Sequencing

Journal: Microorganisms

Article Title: Role of FAD-I in Fusobacterial Interspecies Interaction and Biofilm Formation

doi: 10.3390/microorganisms8010070

Figure Lengend Snippet: Schematic representation of gene inactivation mutants of the radD -encoding four-gene operon and controls ( A ) wild-type (WT), ( B ) Δ rapA , ( C ) Δ rapB , ( D ) Δ fad-I , ( E ) Δ radD ( F ), and wild-type- catP insertion control (WT_CIC) in F. nucleatum subspecies nucleatum and polymorphum . ( G ) Δ fad-I radD *: F. nucleatum ssp. nucleatum ATCC 23726 radD frameshift mutant in the Δ fad - I background.

Article Snippet: , Fnp_Δ radD , ATCC 10953::pBS24 , [ ] .

Techniques: Control, Mutagenesis

Journal: Microorganisms

Article Title: Role of FAD-I in Fusobacterial Interspecies Interaction and Biofilm Formation

doi: 10.3390/microorganisms8010070

Figure Lengend Snippet: Quantitative Coaggregation of wild-type F. nucleatum ssp. nucleatum (ATCC 23726) and F. nucleatum ssp. polymorphum (ATCC 10953) and their various mutant derivatives: Δ rapA , Δ rapB , Δ fad - I , Δ radD , and control (WT_CIC) with S. gordonii. Data are represented as mean of percentage coaggregation and standard error of mean of three independent experiments. (** p ≤ 0.01 and *** p ≤ 0.001 compared to the wild-type control).

Article Snippet: , Fnp_Δ radD , ATCC 10953::pBS24 , [ ] .

Techniques: Mutagenesis, Control

Journal: Microorganisms

Article Title: Role of FAD-I in Fusobacterial Interspecies Interaction and Biofilm Formation

doi: 10.3390/microorganisms8010070

Figure Lengend Snippet: Transcriptional analysis of radD -operon genes in wild-type F. nucleatum ssp. nucleatum (ATCC 23726) and F. nucleatum ssp. polymorphum (ATCC 10953) and their mutant derivatives. Expression fold changes compared to wild-type are shown for ( A ) rapA , ( B ) rapB , ( C ) fad-I , and ( D ) radD for mutant derivatives and the WT_CIC control strains. Data are presented as the mean and standard error of mean of three independent experiments. (*** represents p ≤ 0.001 compared to the wild-type control).

Article Snippet: , Fnp_Δ radD , ATCC 10953::pBS24 , [ ] .

Techniques: Mutagenesis, Expressing, Control

Journal: Microorganisms

Article Title: Role of FAD-I in Fusobacterial Interspecies Interaction and Biofilm Formation

doi: 10.3390/microorganisms8010070

Figure Lengend Snippet: Characterization of the fad - I complement of F. nucleatum ssp . nucleatum. ( A ) Quantitative coaggregation of the mutant derivatives of wild-type ATCC 23726 (WT), Fnn_Δ fad -I, and Fnn_Δ fad -I/pBS5 with S. gordonii is shown as percentage coaggregation. ( B ) Transcriptional levels of radD in the Fnn_Δ fad -I mutant and the Fnn_Δ fad -I/pBS5 complement along with a vector only control WT/pHS58 are shown as fold change in comparison to the wild-type parent ATCC 23726. All data are presented as the mean and standard error of mean of three independent experiments (** represents p ≤ 0.01).

Article Snippet: , Fnp_Δ radD , ATCC 10953::pBS24 , [ ] .

Techniques: Mutagenesis, Plasmid Preparation, Control, Comparison

Journal: Microorganisms

Article Title: Role of FAD-I in Fusobacterial Interspecies Interaction and Biofilm Formation

doi: 10.3390/microorganisms8010070

Figure Lengend Snippet: Quantitative coaggregation and transcriptional analysis of F. nucleatum ssp . nucleatum , Fnn_Δ fad-I , and Fnn_Δ fad-IradD*. ( A ) Coaggregation of wild-type ATCC 23726 (WT), Fnn_Δ fad-I , and Fnn_Δ fad-IradD* with S. gordonii is represented as mean of percentage coaggregation and standard error of mean of three independent experiments. ( B ) fad-I and radD transcript levels are represented as expression fold change compared to the wild-type parent ATCC 23726. The data represent the mean and standard error of mean of three independent experiments. (** represents p ≤ 0.01 compared to the WT control, *** represents p ≤ 0.001 than the WT).

Article Snippet: , Fnp_Δ radD , ATCC 10953::pBS24 , [ ] .

Techniques: Expressing, Control

Journal: Microorganisms

Article Title: Role of FAD-I in Fusobacterial Interspecies Interaction and Biofilm Formation

doi: 10.3390/microorganisms8010070

Figure Lengend Snippet: Transcriptional analysis of radD levels in the presence of S. gordonii . Presented are radD levels in ( A ) wild-type ATCC 23726 (WT), ( B ) Fnn_Δ fad-I , and ( C ) Fnn_Δ radD alone or in the presence of the partner species. Data are presented as the mean and standard error of mean of three independent experiments (* represents p ≤ 0.05, ** represents p ≤ 0.01, *** represents p ≤ 0.001).

Article Snippet: , Fnp_Δ radD , ATCC 10953::pBS24 , [ ] .

Techniques:

Journal: Microorganisms

Article Title: Role of FAD-I in Fusobacterial Interspecies Interaction and Biofilm Formation

doi: 10.3390/microorganisms8010070

Figure Lengend Snippet: Biomass of dual-species biofilms formed by F. nucleatum ssp nucleatum ATCC 23726 and its radD -operon mutant derivatives with S. gordonii . The biomass was assessed via the crystal violet assay. Data are presented as the mean and standard error of mean of three independent experiments (*** represents p ≤ 0.001).

Article Snippet: , Fnp_Δ radD , ATCC 10953::pBS24 , [ ] .

Techniques: Mutagenesis, Crystal Violet Assay

Journal: Microorganisms

Article Title: Role of FAD-I in Fusobacterial Interspecies Interaction and Biofilm Formation

doi: 10.3390/microorganisms8010070

Figure Lengend Snippet: ( A ). Visualization of dual-species biofilm formed between wild-type and mutant strains of F. nucleatum ssp nucleatum ATCC 23726 and S. gordonii (mCherry) by CLSM. The biofilm was fluorescently labeled with SYTO9. The S. gordonii (Sg) cells constitutively express mCherry and appear red on the images. Wild-type (WT) F. nucleatum (Fn) and its mutants ( fad-I, fad-I/pBS 5, radD , WT-CIC) are stained by syto9-only which are pseudo-colored as blue in the Zen software. Association of F. nucleatum and S. gordonii in the biofilm is observed as purple color in the confocal images. Each image panel is represented by x-z axis view on top and y-z axis view on the right side of the x-y view. The various panels show the biofilm formed by: ( 1 ) S. gordonii (Sg) alone; ( 2 ) wild-type ATCC 23726 with S. gordonii; ( 3 ) Fnn_Δ fad-I with S. gordonii; ( 4 ) Fnn_Δ fad-I /pBS5 with S. gordonii ; ( 5 ) Fnn_Δ radD with S. gordonii; ( 6 ) Fnn_WT_CIC with S. gordonii . ( B ) Comparison of the height of the dual species biofilm of the wild-type and mutant strains of F. nucleatum ssp nucleatum ATCC 23726 with S. gordonii , as observed from the confocal images. The data represents mean of the height and standard error of mean of biofilm as observed in three independent experiments with height measurements captured in five randomly chosen locations in each experiment (n = 15). The single species biofilm of S. gordonii is also included as control. (* represents p ≤ 0.05, ** represents p ≤ 0.01).

Article Snippet: , Fnp_Δ radD , ATCC 10953::pBS24 , [ ] .

Techniques: Mutagenesis, Labeling, Staining, Software, Comparison, Control

Journal: Microorganisms

Article Title: Role of FAD-I in Fusobacterial Interspecies Interaction and Biofilm Formation

doi: 10.3390/microorganisms8010070

Figure Lengend Snippet: Bacterial strains and plasmids used in the study.

Article Snippet: , Fnn_Δ radD , ATCC 23726::pIP1526 , [ ] .

Techniques: Plasmid Preparation

Journal: Microorganisms

Article Title: Role of FAD-I in Fusobacterial Interspecies Interaction and Biofilm Formation

doi: 10.3390/microorganisms8010070

Figure Lengend Snippet: List of qPCR primers used in the study.

Article Snippet: , Fnn_Δ radD , ATCC 23726::pIP1526 , [ ] .

Techniques: Sequencing

Journal: Microorganisms

Article Title: Role of FAD-I in Fusobacterial Interspecies Interaction and Biofilm Formation

doi: 10.3390/microorganisms8010070

Figure Lengend Snippet: Schematic representation of gene inactivation mutants of the radD -encoding four-gene operon and controls ( A ) wild-type (WT), ( B ) Δ rapA , ( C ) Δ rapB , ( D ) Δ fad-I , ( E ) Δ radD ( F ), and wild-type- catP insertion control (WT_CIC) in F. nucleatum subspecies nucleatum and polymorphum . ( G ) Δ fad-I radD *: F. nucleatum ssp. nucleatum ATCC 23726 radD frameshift mutant in the Δ fad - I background.

Article Snippet: , Fnn_Δ radD , ATCC 23726::pIP1526 , [ ] .

Techniques: Control, Mutagenesis

Journal: Microorganisms

Article Title: Role of FAD-I in Fusobacterial Interspecies Interaction and Biofilm Formation

doi: 10.3390/microorganisms8010070

Figure Lengend Snippet: Quantitative Coaggregation of wild-type F. nucleatum ssp. nucleatum (ATCC 23726) and F. nucleatum ssp. polymorphum (ATCC 10953) and their various mutant derivatives: Δ rapA , Δ rapB , Δ fad - I , Δ radD , and control (WT_CIC) with S. gordonii. Data are represented as mean of percentage coaggregation and standard error of mean of three independent experiments. (** p ≤ 0.01 and *** p ≤ 0.001 compared to the wild-type control).

Article Snippet: , Fnn_Δ radD , ATCC 23726::pIP1526 , [ ] .

Techniques: Mutagenesis, Control

Journal: Microorganisms

Article Title: Role of FAD-I in Fusobacterial Interspecies Interaction and Biofilm Formation

doi: 10.3390/microorganisms8010070

Figure Lengend Snippet: Transcriptional analysis of radD -operon genes in wild-type F. nucleatum ssp. nucleatum (ATCC 23726) and F. nucleatum ssp. polymorphum (ATCC 10953) and their mutant derivatives. Expression fold changes compared to wild-type are shown for ( A ) rapA , ( B ) rapB , ( C ) fad-I , and ( D ) radD for mutant derivatives and the WT_CIC control strains. Data are presented as the mean and standard error of mean of three independent experiments. (*** represents p ≤ 0.001 compared to the wild-type control).

Article Snippet: , Fnn_Δ radD , ATCC 23726::pIP1526 , [ ] .

Techniques: Mutagenesis, Expressing, Control

Journal: Microorganisms

Article Title: Role of FAD-I in Fusobacterial Interspecies Interaction and Biofilm Formation

doi: 10.3390/microorganisms8010070

Figure Lengend Snippet: Characterization of the fad - I complement of F. nucleatum ssp . nucleatum. ( A ) Quantitative coaggregation of the mutant derivatives of wild-type ATCC 23726 (WT), Fnn_Δ fad -I, and Fnn_Δ fad -I/pBS5 with S. gordonii is shown as percentage coaggregation. ( B ) Transcriptional levels of radD in the Fnn_Δ fad -I mutant and the Fnn_Δ fad -I/pBS5 complement along with a vector only control WT/pHS58 are shown as fold change in comparison to the wild-type parent ATCC 23726. All data are presented as the mean and standard error of mean of three independent experiments (** represents p ≤ 0.01).

Article Snippet: , Fnn_Δ radD , ATCC 23726::pIP1526 , [ ] .

Techniques: Mutagenesis, Plasmid Preparation, Control, Comparison

Journal: Microorganisms

Article Title: Role of FAD-I in Fusobacterial Interspecies Interaction and Biofilm Formation

doi: 10.3390/microorganisms8010070

Figure Lengend Snippet: Quantitative coaggregation and transcriptional analysis of F. nucleatum ssp . nucleatum , Fnn_Δ fad-I , and Fnn_Δ fad-IradD*. ( A ) Coaggregation of wild-type ATCC 23726 (WT), Fnn_Δ fad-I , and Fnn_Δ fad-IradD* with S. gordonii is represented as mean of percentage coaggregation and standard error of mean of three independent experiments. ( B ) fad-I and radD transcript levels are represented as expression fold change compared to the wild-type parent ATCC 23726. The data represent the mean and standard error of mean of three independent experiments. (** represents p ≤ 0.01 compared to the WT control, *** represents p ≤ 0.001 than the WT).

Article Snippet: , Fnn_Δ radD , ATCC 23726::pIP1526 , [ ] .

Techniques: Expressing, Control

Journal: Microorganisms

Article Title: Role of FAD-I in Fusobacterial Interspecies Interaction and Biofilm Formation

doi: 10.3390/microorganisms8010070

Figure Lengend Snippet: Transcriptional analysis of radD levels in the presence of S. gordonii . Presented are radD levels in ( A ) wild-type ATCC 23726 (WT), ( B ) Fnn_Δ fad-I , and ( C ) Fnn_Δ radD alone or in the presence of the partner species. Data are presented as the mean and standard error of mean of three independent experiments (* represents p ≤ 0.05, ** represents p ≤ 0.01, *** represents p ≤ 0.001).

Article Snippet: , Fnn_Δ radD , ATCC 23726::pIP1526 , [ ] .

Techniques:

Journal: Microorganisms

Article Title: Role of FAD-I in Fusobacterial Interspecies Interaction and Biofilm Formation

doi: 10.3390/microorganisms8010070

Figure Lengend Snippet: Biomass of dual-species biofilms formed by F. nucleatum ssp nucleatum ATCC 23726 and its radD -operon mutant derivatives with S. gordonii . The biomass was assessed via the crystal violet assay. Data are presented as the mean and standard error of mean of three independent experiments (*** represents p ≤ 0.001).

Article Snippet: , Fnn_Δ radD , ATCC 23726::pIP1526 , [ ] .

Techniques: Mutagenesis, Crystal Violet Assay

Journal: Microorganisms

Article Title: Role of FAD-I in Fusobacterial Interspecies Interaction and Biofilm Formation

doi: 10.3390/microorganisms8010070

Figure Lengend Snippet: ( A ). Visualization of dual-species biofilm formed between wild-type and mutant strains of F. nucleatum ssp nucleatum ATCC 23726 and S. gordonii (mCherry) by CLSM. The biofilm was fluorescently labeled with SYTO9. The S. gordonii (Sg) cells constitutively express mCherry and appear red on the images. Wild-type (WT) F. nucleatum (Fn) and its mutants ( fad-I, fad-I/pBS 5, radD , WT-CIC) are stained by syto9-only which are pseudo-colored as blue in the Zen software. Association of F. nucleatum and S. gordonii in the biofilm is observed as purple color in the confocal images. Each image panel is represented by x-z axis view on top and y-z axis view on the right side of the x-y view. The various panels show the biofilm formed by: ( 1 ) S. gordonii (Sg) alone; ( 2 ) wild-type ATCC 23726 with S. gordonii; ( 3 ) Fnn_Δ fad-I with S. gordonii; ( 4 ) Fnn_Δ fad-I /pBS5 with S. gordonii ; ( 5 ) Fnn_Δ radD with S. gordonii; ( 6 ) Fnn_WT_CIC with S. gordonii . ( B ) Comparison of the height of the dual species biofilm of the wild-type and mutant strains of F. nucleatum ssp nucleatum ATCC 23726 with S. gordonii , as observed from the confocal images. The data represents mean of the height and standard error of mean of biofilm as observed in three independent experiments with height measurements captured in five randomly chosen locations in each experiment (n = 15). The single species biofilm of S. gordonii is also included as control. (* represents p ≤ 0.05, ** represents p ≤ 0.01).

Article Snippet: , Fnn_Δ radD , ATCC 23726::pIP1526 , [ ] .

Techniques: Mutagenesis, Labeling, Staining, Software, Comparison, Control