rabbit anti rab35  (Proteintech)

Journal: Marine Drugs

Article Title: Moisturizing Effects of Alcalase Hydrolysate Fractions from Haliotis discus Viscera, a Marine Organism, on Human Dermal Fibroblasts, HaCaT Keratinocytes, and Reconstructed Human Skin Tissues

doi: 10.3390/md22110503

Figure Lengend Snippet: Effect of Al-Fr.1 via the MAPK signaling pathway in HaCaT cells. Cells were treated with Al-Fr.1 at concentrations of 12.5, 25, and 50 µg/mL for 24 h. Protein levels of p-ERK, ERK, p-JNK, JNK, p-p38, p38, and β-actin were analyzed using Western blot analysis. Quantitative analysis was performed using ImageJ software. Values are expressed as the mean ± SD of triplicate experiments. Different lowercase letters indicate significant differences.

Article Snippet: The primary antibodies against TGFβ (3711), p-smad2 (8828), smad2 (8685), ERK (4695), p-JNK (9251), JNK (9252), p-p38 (4511), and p38 (9690) were obtained from Cell Signaling Technology (Beverly, MA, USA), and p-ERK (sc-7383) and β-actin (sc-47778) were purchased from Santacruz (Dallas, TX, USA), and were diluted at a 1:1000 ratio.

Techniques: Western Blot, Software