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ra839  (Tocris)


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    Tocris ra839
    DENV replication suppresses ROS in differentiating cells by upregulation of NFE2L2 activity. (A) K562 cells either uninfected or infected with DENV at an MOI of 1.0 were differentiated with 50 nM PMA in the absence or presence of 10 μM of <t>RA839.</t> At 3 or 6 days post-infection the cytosolic ROS was quantified by H 2 DCFDA staining as described above. (B) K562 cells infected with DENV at an MOI of 1.0 were differentiated with PMA in the absence or presence of 10 μM of RA839. The supernatant was collected at 3 or 6 days post-infection and the infectious titer of secreted virus quantified by Focus-forming unit (FFU) assay. The Log 10 of the FFU/ml was calculated and plotted. (C) K562 cells either uninfected or infected with DENV at an MOI of 1.0 were differentiated with PMA in the absence or presence of 10 μM of ML385. At 3 or 6 days post-infection the cytosolic ROS was quantified by H 2 DCFDA staining as described above. (D) K562 cells infected with DENV at an MOI of 1.0 were differentiated with PMA in the absence or presence of 10 μM of ML385. The culture supernatant was collected at 3 or 6 days post-infection and the infectious titer of secreted virus quantified by Focus-forming unit (FFU) assay. The Log 10 of the FFU/ml was calculated and plotted. (A–D) The error bars represent standard deviation obtained from 3 independent experiments and the P -values of significance calculated by Student's t -test are indicated.
    Ra839, supplied by Tocris, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ra839/pmc08787197-137-14-15?v=Tocris
    Average 92 stars, based on 8 article reviews
    ra839 - by Bioz Stars, 2026-08
    92/100 stars

    Images

    1) Product Images from "Replication of Dengue Virus in K562-Megakaryocytes Induces Suppression in the Accumulation of Reactive Oxygen Species"

    Article Title: Replication of Dengue Virus in K562-Megakaryocytes Induces Suppression in the Accumulation of Reactive Oxygen Species

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2021.784070

    DENV replication suppresses ROS in differentiating cells by upregulation of NFE2L2 activity. (A) K562 cells either uninfected or infected with DENV at an MOI of 1.0 were differentiated with 50 nM PMA in the absence or presence of 10 μM of RA839. At 3 or 6 days post-infection the cytosolic ROS was quantified by H 2 DCFDA staining as described above. (B) K562 cells infected with DENV at an MOI of 1.0 were differentiated with PMA in the absence or presence of 10 μM of RA839. The supernatant was collected at 3 or 6 days post-infection and the infectious titer of secreted virus quantified by Focus-forming unit (FFU) assay. The Log 10 of the FFU/ml was calculated and plotted. (C) K562 cells either uninfected or infected with DENV at an MOI of 1.0 were differentiated with PMA in the absence or presence of 10 μM of ML385. At 3 or 6 days post-infection the cytosolic ROS was quantified by H 2 DCFDA staining as described above. (D) K562 cells infected with DENV at an MOI of 1.0 were differentiated with PMA in the absence or presence of 10 μM of ML385. The culture supernatant was collected at 3 or 6 days post-infection and the infectious titer of secreted virus quantified by Focus-forming unit (FFU) assay. The Log 10 of the FFU/ml was calculated and plotted. (A–D) The error bars represent standard deviation obtained from 3 independent experiments and the P -values of significance calculated by Student's t -test are indicated.
    Figure Legend Snippet: DENV replication suppresses ROS in differentiating cells by upregulation of NFE2L2 activity. (A) K562 cells either uninfected or infected with DENV at an MOI of 1.0 were differentiated with 50 nM PMA in the absence or presence of 10 μM of RA839. At 3 or 6 days post-infection the cytosolic ROS was quantified by H 2 DCFDA staining as described above. (B) K562 cells infected with DENV at an MOI of 1.0 were differentiated with PMA in the absence or presence of 10 μM of RA839. The supernatant was collected at 3 or 6 days post-infection and the infectious titer of secreted virus quantified by Focus-forming unit (FFU) assay. The Log 10 of the FFU/ml was calculated and plotted. (C) K562 cells either uninfected or infected with DENV at an MOI of 1.0 were differentiated with PMA in the absence or presence of 10 μM of ML385. At 3 or 6 days post-infection the cytosolic ROS was quantified by H 2 DCFDA staining as described above. (D) K562 cells infected with DENV at an MOI of 1.0 were differentiated with PMA in the absence or presence of 10 μM of ML385. The culture supernatant was collected at 3 or 6 days post-infection and the infectious titer of secreted virus quantified by Focus-forming unit (FFU) assay. The Log 10 of the FFU/ml was calculated and plotted. (A–D) The error bars represent standard deviation obtained from 3 independent experiments and the P -values of significance calculated by Student's t -test are indicated.

    Techniques Used: Activity Assay, Infection, Staining, Virus, Standard Deviation



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    ra839  (Tocris)
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    Tocris ra839
    DENV replication suppresses ROS in differentiating cells by upregulation of NFE2L2 activity. (A) K562 cells either uninfected or infected with DENV at an MOI of 1.0 were differentiated with 50 nM PMA in the absence or presence of 10 μM of <t>RA839.</t> At 3 or 6 days post-infection the cytosolic ROS was quantified by H 2 DCFDA staining as described above. (B) K562 cells infected with DENV at an MOI of 1.0 were differentiated with PMA in the absence or presence of 10 μM of RA839. The supernatant was collected at 3 or 6 days post-infection and the infectious titer of secreted virus quantified by Focus-forming unit (FFU) assay. The Log 10 of the FFU/ml was calculated and plotted. (C) K562 cells either uninfected or infected with DENV at an MOI of 1.0 were differentiated with PMA in the absence or presence of 10 μM of ML385. At 3 or 6 days post-infection the cytosolic ROS was quantified by H 2 DCFDA staining as described above. (D) K562 cells infected with DENV at an MOI of 1.0 were differentiated with PMA in the absence or presence of 10 μM of ML385. The culture supernatant was collected at 3 or 6 days post-infection and the infectious titer of secreted virus quantified by Focus-forming unit (FFU) assay. The Log 10 of the FFU/ml was calculated and plotted. (A–D) The error bars represent standard deviation obtained from 3 independent experiments and the P -values of significance calculated by Student's t -test are indicated.
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    Image Search Results


    DENV replication suppresses ROS in differentiating cells by upregulation of NFE2L2 activity. (A) K562 cells either uninfected or infected with DENV at an MOI of 1.0 were differentiated with 50 nM PMA in the absence or presence of 10 μM of RA839. At 3 or 6 days post-infection the cytosolic ROS was quantified by H 2 DCFDA staining as described above. (B) K562 cells infected with DENV at an MOI of 1.0 were differentiated with PMA in the absence or presence of 10 μM of RA839. The supernatant was collected at 3 or 6 days post-infection and the infectious titer of secreted virus quantified by Focus-forming unit (FFU) assay. The Log 10 of the FFU/ml was calculated and plotted. (C) K562 cells either uninfected or infected with DENV at an MOI of 1.0 were differentiated with PMA in the absence or presence of 10 μM of ML385. At 3 or 6 days post-infection the cytosolic ROS was quantified by H 2 DCFDA staining as described above. (D) K562 cells infected with DENV at an MOI of 1.0 were differentiated with PMA in the absence or presence of 10 μM of ML385. The culture supernatant was collected at 3 or 6 days post-infection and the infectious titer of secreted virus quantified by Focus-forming unit (FFU) assay. The Log 10 of the FFU/ml was calculated and plotted. (A–D) The error bars represent standard deviation obtained from 3 independent experiments and the P -values of significance calculated by Student's t -test are indicated.

    Journal: Frontiers in Microbiology

    Article Title: Replication of Dengue Virus in K562-Megakaryocytes Induces Suppression in the Accumulation of Reactive Oxygen Species

    doi: 10.3389/fmicb.2021.784070

    Figure Lengend Snippet: DENV replication suppresses ROS in differentiating cells by upregulation of NFE2L2 activity. (A) K562 cells either uninfected or infected with DENV at an MOI of 1.0 were differentiated with 50 nM PMA in the absence or presence of 10 μM of RA839. At 3 or 6 days post-infection the cytosolic ROS was quantified by H 2 DCFDA staining as described above. (B) K562 cells infected with DENV at an MOI of 1.0 were differentiated with PMA in the absence or presence of 10 μM of RA839. The supernatant was collected at 3 or 6 days post-infection and the infectious titer of secreted virus quantified by Focus-forming unit (FFU) assay. The Log 10 of the FFU/ml was calculated and plotted. (C) K562 cells either uninfected or infected with DENV at an MOI of 1.0 were differentiated with PMA in the absence or presence of 10 μM of ML385. At 3 or 6 days post-infection the cytosolic ROS was quantified by H 2 DCFDA staining as described above. (D) K562 cells infected with DENV at an MOI of 1.0 were differentiated with PMA in the absence or presence of 10 μM of ML385. The culture supernatant was collected at 3 or 6 days post-infection and the infectious titer of secreted virus quantified by Focus-forming unit (FFU) assay. The Log 10 of the FFU/ml was calculated and plotted. (A–D) The error bars represent standard deviation obtained from 3 independent experiments and the P -values of significance calculated by Student's t -test are indicated.

    Article Snippet: Phorbol-12 Myristate-13 acetate (PMA, Sigma Aldrich), N-Acetyl Cysteine (NAC, Sigma Aldrich), NITD008 (Sigma Aldrich), RA839 (Tocris), ML385 and Sodium Butyrate (Sigma Aldrich) were diluted in recommended vehicles and stored as single use aliquots at −20°C.

    Techniques: Activity Assay, Infection, Staining, Virus, Standard Deviation