Sequencing:Article Title: Secreted proteins and polynucleotides encoding them
Article Snippet: .. In other embodiments, the present invention provides a composition comprising a protein, wherein said protein comprises an amino acid sequence selected from the group consisting of: (a) the amino acid sequence of SEQ ID NO:32; (b) a fragment of the amino acid sequence of SEQ ID NO:32, the fragment comprising eight contiguous amino acids of SEQ ID NO:32; and (c) the amino acid sequence encoded by the cDNA insert of clone pv35—1 deposited under accession number ATCC 98835; the protein being substantially free from other mammalian proteins. ..
Article Title: Secreted proteins and polynucleotides encoding them
Article Snippet: .. In one embodiment, the present invention provides a composition comprising an isolated polynucleotide selected from the group consisting of: (a) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:31; (b) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:31 from nucleotide 158 to nucleotide 1102; (c) a polynucleotide comprising the nucleotide sequence of the full-length protein coding sequence of clone pv35—1 deposited under accession number ATCC 98835; (d) a polynucleotide encoding the full-length protein encoded by the cDNA insert of clone pv35—1 deposited under accession number ATCC 98835; (e) a polynucleotide comprising the nucleotide sequence of a mature protein coding sequence of clone pv35—1 deposited under accession number ATCC 98835; (f) a polynucleotide encoding a mature protein encoded by the cDNA insert of clone pv35—1 deposited under accession number ATCC 98835; (g) a polynucleotide encoding a protein comprising the amino acid sequence of SEQ ID NO:32; (h) a polynucleotide encoding a protein comprising a fragment of the amino acid sequence of SEQ ID NO:32 having biological activity, the fragment comprising eight contiguous amino acids of SEQ ID NO:32; (i) a polynucleotide which is an allelic variant of a polynucleotide of (a)-(f) above; (j) a polynucleotide which encodes a species homologue of the protein of (g) or (h) above; (k) a polynucleotide that hybridizes under stringent conditions to any one of the polynucleotides specified in (a)-(h); and (l) a polynucleotide that hybridizes under stringent conditions to any one of the polynucleotides specified in (a)-(h) and that has a length that is at least 25% of the length of SEQ ID NO:31. .. Preferably, such polynucleotide comprises the nucleotide sequence of SEQ ID NO:31 from nucleotide 158 to nucleotide 1102; the nucleotide sequence of the fun-length protein coding sequence of clone pv35—1 deposited under accession number ATCC 98835; or the nucleotide sequence of a mature protein coding sequence of clone pv35—1 deposited under accession number ATCC 98835.
Article Title: Secreted proteins and polynucleotides encoding them
Article Snippet: .. Further embodiments of the invention provide isolated polynucleotides produced according to a process selected from the group consisting of: (a) a process comprising the steps of: (i) preparing one or more polynucleotide probes that hybridize in 6×SSC at 65 degrees C. to a nucleotide sequence selected from the group consisting of: (aa) SEQ ID NO:31, but excluding the poly(A) tail at the 3′ end of SEQ ID NO:31; and (ab) the nucleotide sequence of the cDNA insert of clone pv35—1 deposited under accession number ATCC 98835; (ii) hybridizing said probe(s) to human genomic DNA in conditions at least as stringent as 4×SSC at 50 degrees C.; and (iii) isolating the DNA polynucleotides detected with the probe(s); and (b) a process comprising the steps of: (i) preparing one or more polynucleotide primers that hybridize in 6×SSC at 65 degrees C. to a nucleotide sequence selected from the group consisting of: (ba) SEQ ID NO:31, but excluding the poly(A) tail at the 3′ end of SEQ ID NO:31; and (bb) the nucleotide sequence of the cDNA insert of clone pv35—1 deposited under accession number ATCC 98835; (ii) hybridizing said primer(s) to human genomic DNA in conditions at least as stringent as 4×SSC at 50 degrees C.; (iii) amplifying human DNA sequences; and (iv) isolating the polynucleotide products of step (b)(iii). ..
Article Title: Secreted proteins and polynucleotides encoding them
Article Snippet: In one embodiment, the present invention provides a composition comprising an isolated polynucleotide selected from the group consisting of: (a) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:31; (b) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:31 from nucleotide 158 to nucleotide 1102; (c) a polynucleotide comprising the nucleotide sequence of the full-length protein coding sequence of clone pv35—1 deposited under accession number ATCC 98835; (d) a polynucleotide encoding the full-length protein encoded by the cDNA insert of clone pv35—1 deposited under accession number ATCC 98835; (e) a polynucleotide comprising the nucleotide sequence of a mature protein coding sequence of clone pv35—1 deposited under accession number ATCC 98835; (f) a polynucleotide encoding a mature protein encoded by the cDNA insert of clone pv35—1 deposited under accession number ATCC 98835; (g) a polynucleotide encoding a protein comprising the amino acid sequence of SEQ ID NO:32; (h) a polynucleotide encoding a protein comprising a fragment of the amino acid sequence of SEQ ID NO:32 having biological activity, the fragment comprising eight contiguous amino acids of SEQ ID NO:32; (i) a polynucleotide which is an allelic variant of a polynucleotide of (a)-(f) above; (j) a polynucleotide which encodes a species homologue of the protein of (g) or (h) above; (k) a polynucleotide that hybridizes under stringent conditions to any one of the polynucleotides specified in (a)-(h); and (l) a polynucleotide that hybridizes under stringent conditions to any one of the polynucleotides specified in (a)-(h) and that has a length that is at least 25% of the length of SEQ ID NO:31. .. Preferably, such polynucleotide comprises the nucleotide sequence of SEQ ID NO:31 from nucleotide 158 to nucleotide 1102; the nucleotide sequence of the fun-length protein coding sequence of clone pv35—1 deposited under accession number ATCC 98835; or the nucleotide sequence of a mature protein coding sequence of clone pv35—1 deposited under accession number ATCC 98835. .. In other preferred embodiments, the polynucleotide encodes the full-length or a mature protein encoded by the cDNA insert of clone pv35—1 deposited under accession number ATCC 98835.
Clone Assay:Article Title: Secreted proteins and polynucleotides encoding them
Article Snippet: 29, 1998 with the American Type Culture Collection. (10801 University Boulevard, Manassas, Va. 20110-2209 U.S.A.) as an original deposit under the Budapest Treaty and were given the accession number ATCC 98825, from which each clone comprising a particular polynucleotide is obtainable. .. Clones ca106—19xx, ci52—2, md124—16, pk366—7, pl741—5, pp314—19, pv35—1, pw337—6, rd610—1, and rd810—6 were deposited on Aug. 11, 1998 with the American Type Culture Collection (10801 University Boulevard, Manassas, Va. 20110-2209 U.S.A.) as an original deposit under the Budapest Treaty and were given the accession number ATCC 98835, from which each clone comprising a particular polynucleotide is obtainable. .. Clones cf85—1, dd504—18, np26—3, pm412—12, pm421—3, pv6—1, qs14—3, qy338—9, rc58—1, and rd232—5 were deposited on Aug. 27, 1998 with the American Type Culture Collection (10801 University Boulevard, Manassas, Va. 20110-2209 U.S.A.) as an original deposit under the Budapest Treaty and were given the accession number ATCC 98850, from which each clone comprising a particular polynucleotide is obtainable.
Isolation:Article Title: Secreted proteins and polynucleotides encoding them
Article Snippet: .. In one embodiment, the present invention provides a composition comprising an isolated polynucleotide selected from the group consisting of: (a) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:31; (b) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:31 from nucleotide 158 to nucleotide 1102; (c) a polynucleotide comprising the nucleotide sequence of the full-length protein coding sequence of clone pv35—1 deposited under accession number ATCC 98835; (d) a polynucleotide encoding the full-length protein encoded by the cDNA insert of clone pv35—1 deposited under accession number ATCC 98835; (e) a polynucleotide comprising the nucleotide sequence of a mature protein coding sequence of clone pv35—1 deposited under accession number ATCC 98835; (f) a polynucleotide encoding a mature protein encoded by the cDNA insert of clone pv35—1 deposited under accession number ATCC 98835; (g) a polynucleotide encoding a protein comprising the amino acid sequence of SEQ ID NO:32; (h) a polynucleotide encoding a protein comprising a fragment of the amino acid sequence of SEQ ID NO:32 having biological activity, the fragment comprising eight contiguous amino acids of SEQ ID NO:32; (i) a polynucleotide which is an allelic variant of a polynucleotide of (a)-(f) above; (j) a polynucleotide which encodes a species homologue of the protein of (g) or (h) above; (k) a polynucleotide that hybridizes under stringent conditions to any one of the polynucleotides specified in (a)-(h); and (l) a polynucleotide that hybridizes under stringent conditions to any one of the polynucleotides specified in (a)-(h) and that has a length that is at least 25% of the length of SEQ ID NO:31. .. Preferably, such polynucleotide comprises the nucleotide sequence of SEQ ID NO:31 from nucleotide 158 to nucleotide 1102; the nucleotide sequence of the fun-length protein coding sequence of clone pv35—1 deposited under accession number ATCC 98835; or the nucleotide sequence of a mature protein coding sequence of clone pv35—1 deposited under accession number ATCC 98835.
Article Title: Secreted proteins and polynucleotides encoding them
Article Snippet: .. Further embodiments of the invention provide isolated polynucleotides produced according to a process selected from the group consisting of: (a) a process comprising the steps of: (i) preparing one or more polynucleotide probes that hybridize in 6×SSC at 65 degrees C. to a nucleotide sequence selected from the group consisting of: (aa) SEQ ID NO:31, but excluding the poly(A) tail at the 3′ end of SEQ ID NO:31; and (ab) the nucleotide sequence of the cDNA insert of clone pv35—1 deposited under accession number ATCC 98835; (ii) hybridizing said probe(s) to human genomic DNA in conditions at least as stringent as 4×SSC at 50 degrees C.; and (iii) isolating the DNA polynucleotides detected with the probe(s); and (b) a process comprising the steps of: (i) preparing one or more polynucleotide primers that hybridize in 6×SSC at 65 degrees C. to a nucleotide sequence selected from the group consisting of: (ba) SEQ ID NO:31, but excluding the poly(A) tail at the 3′ end of SEQ ID NO:31; and (bb) the nucleotide sequence of the cDNA insert of clone pv35—1 deposited under accession number ATCC 98835; (ii) hybridizing said primer(s) to human genomic DNA in conditions at least as stringent as 4×SSC at 50 degrees C.; (iii) amplifying human DNA sequences; and (iv) isolating the polynucleotide products of step (b)(iii). ..
Activity Assay:Article Title: Secreted proteins and polynucleotides encoding them
Article Snippet: .. In one embodiment, the present invention provides a composition comprising an isolated polynucleotide selected from the group consisting of: (a) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:31; (b) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:31 from nucleotide 158 to nucleotide 1102; (c) a polynucleotide comprising the nucleotide sequence of the full-length protein coding sequence of clone pv35—1 deposited under accession number ATCC 98835; (d) a polynucleotide encoding the full-length protein encoded by the cDNA insert of clone pv35—1 deposited under accession number ATCC 98835; (e) a polynucleotide comprising the nucleotide sequence of a mature protein coding sequence of clone pv35—1 deposited under accession number ATCC 98835; (f) a polynucleotide encoding a mature protein encoded by the cDNA insert of clone pv35—1 deposited under accession number ATCC 98835; (g) a polynucleotide encoding a protein comprising the amino acid sequence of SEQ ID NO:32; (h) a polynucleotide encoding a protein comprising a fragment of the amino acid sequence of SEQ ID NO:32 having biological activity, the fragment comprising eight contiguous amino acids of SEQ ID NO:32; (i) a polynucleotide which is an allelic variant of a polynucleotide of (a)-(f) above; (j) a polynucleotide which encodes a species homologue of the protein of (g) or (h) above; (k) a polynucleotide that hybridizes under stringent conditions to any one of the polynucleotides specified in (a)-(h); and (l) a polynucleotide that hybridizes under stringent conditions to any one of the polynucleotides specified in (a)-(h) and that has a length that is at least 25% of the length of SEQ ID NO:31. .. Preferably, such polynucleotide comprises the nucleotide sequence of SEQ ID NO:31 from nucleotide 158 to nucleotide 1102; the nucleotide sequence of the fun-length protein coding sequence of clone pv35—1 deposited under accession number ATCC 98835; or the nucleotide sequence of a mature protein coding sequence of clone pv35—1 deposited under accession number ATCC 98835.
Variant Assay:Article Title: Secreted proteins and polynucleotides encoding them
Article Snippet: .. In one embodiment, the present invention provides a composition comprising an isolated polynucleotide selected from the group consisting of: (a) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:31; (b) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:31 from nucleotide 158 to nucleotide 1102; (c) a polynucleotide comprising the nucleotide sequence of the full-length protein coding sequence of clone pv35—1 deposited under accession number ATCC 98835; (d) a polynucleotide encoding the full-length protein encoded by the cDNA insert of clone pv35—1 deposited under accession number ATCC 98835; (e) a polynucleotide comprising the nucleotide sequence of a mature protein coding sequence of clone pv35—1 deposited under accession number ATCC 98835; (f) a polynucleotide encoding a mature protein encoded by the cDNA insert of clone pv35—1 deposited under accession number ATCC 98835; (g) a polynucleotide encoding a protein comprising the amino acid sequence of SEQ ID NO:32; (h) a polynucleotide encoding a protein comprising a fragment of the amino acid sequence of SEQ ID NO:32 having biological activity, the fragment comprising eight contiguous amino acids of SEQ ID NO:32; (i) a polynucleotide which is an allelic variant of a polynucleotide of (a)-(f) above; (j) a polynucleotide which encodes a species homologue of the protein of (g) or (h) above; (k) a polynucleotide that hybridizes under stringent conditions to any one of the polynucleotides specified in (a)-(h); and (l) a polynucleotide that hybridizes under stringent conditions to any one of the polynucleotides specified in (a)-(h) and that has a length that is at least 25% of the length of SEQ ID NO:31. .. Preferably, such polynucleotide comprises the nucleotide sequence of SEQ ID NO:31 from nucleotide 158 to nucleotide 1102; the nucleotide sequence of the fun-length protein coding sequence of clone pv35—1 deposited under accession number ATCC 98835; or the nucleotide sequence of a mature protein coding sequence of clone pv35—1 deposited under accession number ATCC 98835.
Produced:Article Title: Secreted proteins and polynucleotides encoding them
Article Snippet: .. Further embodiments of the invention provide isolated polynucleotides produced according to a process selected from the group consisting of: (a) a process comprising the steps of: (i) preparing one or more polynucleotide probes that hybridize in 6×SSC at 65 degrees C. to a nucleotide sequence selected from the group consisting of: (aa) SEQ ID NO:31, but excluding the poly(A) tail at the 3′ end of SEQ ID NO:31; and (ab) the nucleotide sequence of the cDNA insert of clone pv35—1 deposited under accession number ATCC 98835; (ii) hybridizing said probe(s) to human genomic DNA in conditions at least as stringent as 4×SSC at 50 degrees C.; and (iii) isolating the DNA polynucleotides detected with the probe(s); and (b) a process comprising the steps of: (i) preparing one or more polynucleotide primers that hybridize in 6×SSC at 65 degrees C. to a nucleotide sequence selected from the group consisting of: (ba) SEQ ID NO:31, but excluding the poly(A) tail at the 3′ end of SEQ ID NO:31; and (bb) the nucleotide sequence of the cDNA insert of clone pv35—1 deposited under accession number ATCC 98835; (ii) hybridizing said primer(s) to human genomic DNA in conditions at least as stringent as 4×SSC at 50 degrees C.; (iii) amplifying human DNA sequences; and (iv) isolating the polynucleotide products of step (b)(iii). ..
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