Journal: The American Journal of Pathology
Article Title: Injured Proximal Tubular Epithelial Cells Lose Hepatocyte Nuclear Factor 4α Expression Crucial for Brush Border Formation and Transport
doi: 10.1016/j.ajpath.2025.01.011
Figure Lengend Snippet: Primary proximal tubular epithelial cells (PTECs) lose expression of proximal tubular markers during culture. PTECs harvested directly following tissue dissociation, preseeding (P0), and at consecutive passages (P1 to P4), followed by immunohistochemical staining, show a rapid reduction of hepatocyte nuclear factor 4α (HNF4A) expression. Expression of the proximal tubular marker CD10 is also down-regulated, but this is more protracted in time. In contrast, the scattered tubular cell marker vimentin (VIM) is rapidly and highly induced in cultured PTECs. n = 3. Scale bars = 100 μm.
Article Snippet: Renal PTECs (RPTECs), purchased from Lonza, harvested at passage 2 to 5 (P2 to P5), and subjected to immunohistochemical staining, show an expression pattern consistent with that in the current cultured primary PTECs [ie, reduced expression of hepatocyte nuclear factor 4α (HNF4A) and CD10, and high vimentin (VIM) expression].
Techniques: Expressing, Immunohistochemical staining, Staining, Marker, Cell Culture