psicheck2 plasmid (Promega)
90
Structured Review
Promega
psicheck2 plasmid
Psicheck2 Plasmid, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/psicheck2/psicheck+2/pmc12268578-308-1-3
Average 90 stars, based on 1 article reviews
Psicheck2 Plasmid, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/psicheck2/psicheck+2/pmc12268578-308-1-3
Average 90 stars, based on 1 article reviews
psicheck2 plasmid - by Bioz Stars,
2026-09
90/100 stars
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Sequencing:Article Title: Pseudogene OCT4-pg5 upregulates OCT4B expression to promote bladder cancer progression by competing with miR-145-5p. Article Snippet: .. The wild-type 3’UTR sequence of OCT4B containing the putative miR-145-5p binding site was cloned into Article Title: Small interfering RNAs generated from the terminal panhandle structure of negative-strand RNA virus promote viral infection. Article Snippet: For viral target validation, the putative target of vsiR-8401 (204 bp sequence from the coding region of RdRP), the putative target of vsiR-7607 (214 bp sequence from the coding region of RdRP) and the putative target of vsiR-5532 (350 bp sequence from viral RNA1 sequence complementary to the RdRP coding region) were cloned and inserted into the luciferase reporter vector psiCHECK2 (Promega; C8021). .. For cellular target validation, the putative target of vsiR-8401 (131 bp sequence from the open reading frame of DDC), the putative target of vsiR-7607 (149 bp sequence from the 3’ UTR of DDC) and the putative target of vsiR5532 (131 bp sequence from the open reading frame of DDC) were also cloned and inserted into Article Title: Small interfering RNAs generated from the terminal panhandle structure of negative-strand RNA virus promote viral infection Article Snippet: For viral target validation, the putative target of vsiR-8401 (204 bp sequence from the coding region of RdRP ), the putative target of vsiR-7607 (214 bp sequence from the coding region of RdRP ) and the putative target of vsiR-5532 (350 bp sequence from viral RNA1 sequence complementary to the RdRP coding region) were cloned and inserted into the luciferase reporter vector psiCHECK2 (Promega; C8021). .. For cellular target validation, the putative target of vsiR-8401 (131 bp sequence from the open reading frame of DDC ), the putative target of vsiR-7607 (149 bp sequence from the 3’ UTR of DDC ) and the putative target of vsiR-5532 (131 bp sequence from the open reading frame of DDC ) were also cloned and inserted into Binding Assay:Article Title: Pseudogene OCT4-pg5 upregulates OCT4B expression to promote bladder cancer progression by competing with miR-145-5p. Article Snippet: .. The wild-type 3’UTR sequence of OCT4B containing the putative miR-145-5p binding site was cloned into Article Title: Suppression of endometriosis by miRNA-34a via inhibition of matrix metalloproteinase-2: An alternative pathway to impede invasion Article Snippet: Densitometry was done for quantification using the software LabImage (Kapelan Bio-Imaging, Leipzig, Germany), and image processing was done on Adobe Photoshop 2021 v22.5.1.441 (San Jose, CA). .. MMP-2 promoter amplification from genomic DNA, isolated from the blood samples of the endometriosis patients, was carried out using the primers, 5′-TT GCGGCCGC GCCATGGGTATGCCGCTGGCCCTGGCTCCCACAGGCCC-3′ (forward), and 5′-CCC CTCGAG CACCTGGCCATGCCCTGTCCC-3′ (reverse), carrying restriction sites for Not I and Xho I respectively (NEB, Massachusetts, US) (the restriction sites are depicted in boldface) was done and the MMP-2 3′-UTR was incorporated in the Clone Assay:Article Title: Pseudogene OCT4-pg5 upregulates OCT4B expression to promote bladder cancer progression by competing with miR-145-5p. Article Snippet: .. The wild-type 3’UTR sequence of OCT4B containing the putative miR-145-5p binding site was cloned into Article Title: Small interfering RNAs generated from the terminal panhandle structure of negative-strand RNA virus promote viral infection. Article Snippet: For viral target validation, the putative target of vsiR-8401 (204 bp sequence from the coding region of RdRP), the putative target of vsiR-7607 (214 bp sequence from the coding region of RdRP) and the putative target of vsiR-5532 (350 bp sequence from viral RNA1 sequence complementary to the RdRP coding region) were cloned and inserted into the luciferase reporter vector psiCHECK2 (Promega; C8021). .. For cellular target validation, the putative target of vsiR-8401 (131 bp sequence from the open reading frame of DDC), the putative target of vsiR-7607 (149 bp sequence from the 3’ UTR of DDC) and the putative target of vsiR5532 (131 bp sequence from the open reading frame of DDC) were also cloned and inserted into Article Title: Small interfering RNAs generated from the terminal panhandle structure of negative-strand RNA virus promote viral infection Article Snippet: For viral target validation, the putative target of vsiR-8401 (204 bp sequence from the coding region of RdRP ), the putative target of vsiR-7607 (214 bp sequence from the coding region of RdRP ) and the putative target of vsiR-5532 (350 bp sequence from viral RNA1 sequence complementary to the RdRP coding region) were cloned and inserted into the luciferase reporter vector psiCHECK2 (Promega; C8021). .. For cellular target validation, the putative target of vsiR-8401 (131 bp sequence from the open reading frame of DDC ), the putative target of vsiR-7607 (149 bp sequence from the 3’ UTR of DDC ) and the putative target of vsiR-5532 (131 bp sequence from the open reading frame of DDC ) were also cloned and inserted into Construct:Article Title: Pseudogene OCT4-pg5 upregulates OCT4B expression to promote bladder cancer progression by competing with miR-145-5p. Article Snippet: .. The wild-type 3’UTR sequence of OCT4B containing the putative miR-145-5p binding site was cloned into Luciferase:Article Title: Pseudogene OCT4-pg5 upregulates OCT4B expression to promote bladder cancer progression by competing with miR-145-5p. Article Snippet: .. The wild-type 3’UTR sequence of OCT4B containing the putative miR-145-5p binding site was cloned into Article Title: Androgen Receptor Promotes Lung Cancer Metastasis by Modifying the miR23a-3p/EPHB2 Pathway. Article Snippet: [Abstract] Objective: This study aimed to investigate the reasons behind the lower survival rates in male lung cancer patients than in female lung cancer patients.. Methods: Through various techniques, such as Argonaute immunoprecipitation, luciferase assays, and ChIP, this study confirmed the positive effects of androgen receptor (AR) on lung cancer cell invasion across different in vitro cell lines and in vivo mouse models.. Results: The findings suggest that AR enhanced the invasion of lung cancer cells by modifying EPHB2 signals at the protein expression level, which in turn required changes in miRNA-23a-3p. Article Title: Suppression of endometriosis by miRNA-34a via inhibition of matrix metalloproteinase-2: An alternative pathway to impede invasion Article Snippet: Densitometry was done for quantification using the software LabImage (Kapelan Bio-Imaging, Leipzig, Germany), and image processing was done on Adobe Photoshop 2021 v22.5.1.441 (San Jose, CA). .. MMP-2 promoter amplification from genomic DNA, isolated from the blood samples of the endometriosis patients, was carried out using the primers, 5′-TT GCGGCCGC GCCATGGGTATGCCGCTGGCCCTGGCTCCCACAGGCCC-3′ (forward), and 5′-CCC CTCGAG CACCTGGCCATGCCCTGTCCC-3′ (reverse), carrying restriction sites for Not I and Xho I respectively (NEB, Massachusetts, US) (the restriction sites are depicted in boldface) was done and the MMP-2 3′-UTR was incorporated in the Control:Article Title: High-Throughput Quantification of miRNA-3′-Untranslated-Region Regulatory Effects Article Snippet: .. For the 3′ UTR control, the unaltered Biomarker Discovery:Article Title: Small interfering RNAs generated from the terminal panhandle structure of negative-strand RNA virus promote viral infection. Article Snippet: For viral target validation, the putative target of vsiR-8401 (204 bp sequence from the coding region of RdRP), the putative target of vsiR-7607 (214 bp sequence from the coding region of RdRP) and the putative target of vsiR-5532 (350 bp sequence from viral RNA1 sequence complementary to the RdRP coding region) were cloned and inserted into the luciferase reporter vector psiCHECK2 (Promega; C8021). .. For cellular target validation, the putative target of vsiR-8401 (131 bp sequence from the open reading frame of DDC), the putative target of vsiR-7607 (149 bp sequence from the 3’ UTR of DDC) and the putative target of vsiR5532 (131 bp sequence from the open reading frame of DDC) were also cloned and inserted into Article Title: Small interfering RNAs generated from the terminal panhandle structure of negative-strand RNA virus promote viral infection Article Snippet: For viral target validation, the putative target of vsiR-8401 (204 bp sequence from the coding region of RdRP ), the putative target of vsiR-7607 (214 bp sequence from the coding region of RdRP ) and the putative target of vsiR-5532 (350 bp sequence from viral RNA1 sequence complementary to the RdRP coding region) were cloned and inserted into the luciferase reporter vector psiCHECK2 (Promega; C8021). .. For cellular target validation, the putative target of vsiR-8401 (131 bp sequence from the open reading frame of DDC ), the putative target of vsiR-7607 (149 bp sequence from the 3’ UTR of DDC ) and the putative target of vsiR-5532 (131 bp sequence from the open reading frame of DDC ) were also cloned and inserted into Amplification:Article Title: Suppression of endometriosis by miRNA-34a via inhibition of matrix metalloproteinase-2: An alternative pathway to impede invasion Article Snippet: Densitometry was done for quantification using the software LabImage (Kapelan Bio-Imaging, Leipzig, Germany), and image processing was done on Adobe Photoshop 2021 v22.5.1.441 (San Jose, CA). .. MMP-2 promoter amplification from genomic DNA, isolated from the blood samples of the endometriosis patients, was carried out using the primers, 5′-TT GCGGCCGC GCCATGGGTATGCCGCTGGCCCTGGCTCCCACAGGCCC-3′ (forward), and 5′-CCC CTCGAG CACCTGGCCATGCCCTGTCCC-3′ (reverse), carrying restriction sites for Not I and Xho I respectively (NEB, Massachusetts, US) (the restriction sites are depicted in boldface) was done and the MMP-2 3′-UTR was incorporated in the Isolation:Article Title: Suppression of endometriosis by miRNA-34a via inhibition of matrix metalloproteinase-2: An alternative pathway to impede invasion Article Snippet: Densitometry was done for quantification using the software LabImage (Kapelan Bio-Imaging, Leipzig, Germany), and image processing was done on Adobe Photoshop 2021 v22.5.1.441 (San Jose, CA). .. MMP-2 promoter amplification from genomic DNA, isolated from the blood samples of the endometriosis patients, was carried out using the primers, 5′-TT GCGGCCGC GCCATGGGTATGCCGCTGGCCCTGGCTCCCACAGGCCC-3′ (forward), and 5′-CCC CTCGAG CACCTGGCCATGCCCTGTCCC-3′ (reverse), carrying restriction sites for Not I and Xho I respectively (NEB, Massachusetts, US) (the restriction sites are depicted in boldface) was done and the MMP-2 3′-UTR was incorporated in the Plasmid Preparation:Article Title: Suppression of endometriosis by miRNA-34a via inhibition of matrix metalloproteinase-2: An alternative pathway to impede invasion Article Snippet: Densitometry was done for quantification using the software LabImage (Kapelan Bio-Imaging, Leipzig, Germany), and image processing was done on Adobe Photoshop 2021 v22.5.1.441 (San Jose, CA). .. MMP-2 promoter amplification from genomic DNA, isolated from the blood samples of the endometriosis patients, was carried out using the primers, 5′-TT GCGGCCGC GCCATGGGTATGCCGCTGGCCCTGGCTCCCACAGGCCC-3′ (forward), and 5′-CCC CTCGAG CACCTGGCCATGCCCTGTCCC-3′ (reverse), carrying restriction sites for Not I and Xho I respectively (NEB, Massachusetts, US) (the restriction sites are depicted in boldface) was done and the MMP-2 3′-UTR was incorporated in the DNA Sequencing:Article Title: Suppression of endometriosis by miRNA-34a via inhibition of matrix metalloproteinase-2: An alternative pathway to impede invasion Article Snippet: Densitometry was done for quantification using the software LabImage (Kapelan Bio-Imaging, Leipzig, Germany), and image processing was done on Adobe Photoshop 2021 v22.5.1.441 (San Jose, CA). .. MMP-2 promoter amplification from genomic DNA, isolated from the blood samples of the endometriosis patients, was carried out using the primers, 5′-TT GCGGCCGC GCCATGGGTATGCCGCTGGCCCTGGCTCCCACAGGCCC-3′ (forward), and 5′-CCC CTCGAG CACCTGGCCATGCCCTGTCCC-3′ (reverse), carrying restriction sites for Not I and Xho I respectively (NEB, Massachusetts, US) (the restriction sites are depicted in boldface) was done and the MMP-2 3′-UTR was incorporated in the |