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Proteintech prrt1
Prrt1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 442 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/psd95/PSD95-Specific%2CDLG4+Antibody/pm41912502-131-82-83
Average 96 stars, based on 442 article reviews
prrt1 - by Bioz Stars, 2026-09
96/100 stars

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Related Articles

Electrophoresis:

Article Title: D-serine ameliorates cognitive deficits by preserving neuronal and synaptic function in experimental anti-NMDAR encephalitis.
Article Snippet: .. After electrophoresis and transfer, membranes were blocked in 5% skim milk prepared in PBST (0.05% Tween-20 in PBS) at room temperature (RT) and incubated overnight at 4 °C with primary antibodies against NMDAR1 (rabbit, 1:1000, AF6406, Affinity Biosciences, RRID: AB_2835236), PSD95 (rabbit, 1:10000, 20665-1- AP, Proteintech, RRID: AB_2687961), SNAP25 (rabbit, 1:1500, A2234, ABclonal, RRID: AB_2753186) and synaptophysin (rabbit, 1:20000, 17785-1-AP, Proteintech, RRID: AB_2271365). .. The membranes were then incubated with a secondary antibody (HRPconjugated goat anti-rabbit IgG, 1:5000, S0001, Affinity Biosciences, RRID: AB_2839429) for 2 hours at RT.

Incubation:

Article Title: D-serine ameliorates cognitive deficits by preserving neuronal and synaptic function in experimental anti-NMDAR encephalitis.
Article Snippet: .. After electrophoresis and transfer, membranes were blocked in 5% skim milk prepared in PBST (0.05% Tween-20 in PBS) at room temperature (RT) and incubated overnight at 4 °C with primary antibodies against NMDAR1 (rabbit, 1:1000, AF6406, Affinity Biosciences, RRID: AB_2835236), PSD95 (rabbit, 1:10000, 20665-1- AP, Proteintech, RRID: AB_2687961), SNAP25 (rabbit, 1:1500, A2234, ABclonal, RRID: AB_2753186) and synaptophysin (rabbit, 1:20000, 17785-1-AP, Proteintech, RRID: AB_2271365). .. The membranes were then incubated with a secondary antibody (HRPconjugated goat anti-rabbit IgG, 1:5000, S0001, Affinity Biosciences, RRID: AB_2839429) for 2 hours at RT.

Article Title: Knockdown of Ddx3x in mPFC induces autistic-like phenotype in mice via altered synaptic plasticity
Article Snippet: .. Proteins were then transferred to a polyvinylidene fluoride (PVDF) membrane (Millipore, No. IPVH00010, USA) and blocked with 5% non-fat milk in tris-buffered saline (TBS: 100 mM Tris, and 1.5 M NaCl, pH 7.6) containing 0.4% (v/v) tween 20 (TBST) for 1 h. The membrane was incubated overnight at 4 °C with primary antibodies against DDX3X (Abcam, No. ab271002, 1:2000, UK), MAPK1 (Abclonal, No. A0229, 1:1000, China), CAMKII-delta (Proteintech, No. 20667-1-AP, 1:1000, China), NMDAR (Huabio, No. JM11-26, 1:600, China), PSD95 (Proteintech, No. 20665-1-AP, 1:1000), PRRT1 (Proteintech, No. 66031-1-lg, 1:600), GAPDH (Abmart, No. M20050 , 1:5000, China), TUBA (Proteintech, No. 66031-1-lg, 1:10000), and VCL (Proteintech, No. 66305-1-lg, 1:15000). .. Secondary antibodies included anti-rabbit (Beyotime, No. A0208, 1:1000, China) and anti-mouse (Abmart, No. M21001 , 1:5000, China), incubated for 1 h at room temperature.

Article Title: Knockdown of Ddx3x in mPFC induces autistic-like phenotype in mice via altered synaptic plasticity.
Article Snippet: .. Proteins were then transferred to a polyvinylidene fluoride (PVDF) membrane (Millipore, No. IPVH00010, USA) and blocked with 5% non-fat milk in tris-buffered saline (TBS: 100mM Tris, and 1.5 M NaCl, pH 7.6) containing 0.4% (v/v) tween 20 (TBST) for 1 h. The membrane was incubated overnight at 4 °C with primary antibodies against DDX3X (Abcam, No. ab271002, 1:2000, UK), MAPK1 (Abclonal, No. A0229, 1:1000, China), CAMKII-delta (Proteintech, No. 20667-1-AP, 1:1000, China), NMDAR (Huabio, No. JM11-26, 1:600, China), PSD95 (Proteintech, No. 20665-1-AP, 1:1000), PRRT1 (Proteintech, No. 66031-1-lg, 1:600), GAPDH (Abmart, No. M20050, 1:5000, China), TUBA (Proteintech, No. 66031-1-lg, 1:10000), and Translational Psychiatry (2026) 16:216 VCL (Proteintech, No. 66305-1-lg, 1:15000). .. Secondary antibodies included anti-rabbit (Beyotime, No. A0208, 1:1000, China) and anti-mouse (Abmart, No. M21001, 1:5000, China), incubated for 1 h at room temperature.

Article Title: Penumbra-derived small extracellular vesicles carry pathogenic RNA driving remote damage after cortical infarction.
Article Snippet: 7 Small extracellular vesicles (sEVs) efficiently transport pathological RNAs in ischemic stroke, 8 yet whether penumbral hypoxia-induced EVs deliver pathogenic cargo to remote regions, 9 impacting acute ischemic stroke outcomes, remains unclear.. 10 Using a focal ischemia mouse model, we isolated brain-derived EVs (BDEVs) from the 11 cortical penumbra and determined their pathological impact on synaptically connected 12 remote regions.. In vitro, penumbra BDEVs (PEVs) exacerbated recipient neuronal damage, 13 intensifying apoptosis and dendritic injury during oxygen-glucose deprivation/reoxygenation.

other:

Article Title: HMGCS2-dependent β-OHB/H3K9bhb ameliorates synaptic plasticity and cognition in Alzheimer’s disease
Article Snippet: PSD95 , PSD95 , Poly- , 1:1000 , Proteintech , 20665-1-AP.

Western Blot:

Article Title: Melatonin Ameliorates decaBDE-Induced Autism-Relevant Behaviors Through Promoting SIRT1/SIRT3/FOXO3a-Dependent Mitochondrial Quality Control.
Article Snippet: Decabromodiphenyl ether (deca-BDE or BDE-209, 98%, CAS#1163-19-5) was obtained from J & K Chemical Technology (Beijing, China); sodium valproate (VPA, powder, 98%, CAS#1069-66-5) and melatonin (MT, powder, ≥98%, CAS#73-31-4) were from SigmaAldrich Trading Co., Ltd. (Shanghai, China); Sirt1/Sir2 inhibitor Selisistat (EX527, 99.85%, CAS#49843-98-3) was from MedChemExpress (Shanghai, China). .. Primary antibodies used for Western blotting were as follows: GAPDH (60004-1-Ig) and PSD95 (20665-1-AP) were from Proteintech (Wuhan, China); SNAP25 (sc-73044), cytochrome c (sc-13156), caspase-3 (sc-56053), COX4 (sc-517553), DRP1 (sc-271583), FIS1 (sc-376447), MFN2 (sc-100560), OPA1 (sc-393296), NRF1 (sc-28379), TFAM (sc-166965), SOD2 (sc-133134), PINK1 (sc-517353), and PRKN (sc-32282) were from Santa Cruz (Dallas, TX, USA); Acetylated-Lysine Antibody (#9441, CST, Danvers, MA, USA); SIRT1 (13161-1-AP), SIRT3 (10099-1-AP), PGC1α (66369-1-Ig), and FOXO3a (66428-1-Ig) were from Proteintech (Wuhan, China). .. Ten-week-old Sprague-Dawley rats (weighing 280 g ± 30 g) were obtained from Changsheng Biotechnology (Liaoning, China, license number: SYXK 2019-0007).

Article Title: Melatonin Ameliorates decaBDE-Induced Autism-Relevant Behaviors Through Promoting SIRT1/SIRT3/FOXO3a-Dependent Mitochondrial Quality Control
Article Snippet: Decabromodiphenyl ether (deca-BDE or BDE-209, 98%, CAS#1163-19-5) was obtained from J & K Chemical Technology (Beijing, China); sodium valproate (VPA, powder, 98%, CAS#1069-66-5) and melatonin (MT, powder, ≥98%, CAS#73-31-4) were from Sigma-Aldrich Trading Co., Ltd. (Shanghai, China); Sirt1/Sir2 inhibitor Selisistat (EX527, 99.85%, CAS#49843-98-3) was from MedChemExpress (Shanghai, China). .. Primary antibodies used for Western blotting were as follows: GAPDH (60004-1-Ig) and PSD95 (20665-1-AP) were from Proteintech (Wuhan, China); SNAP25 (sc-73044), cytochrome c (sc-13156), caspase-3 (sc-56053), COX4 (sc-517553), DRP1 (sc-271583), FIS1 (sc-376447), MFN2 (sc-100560), OPA1 (sc-393296), NRF1 (sc-28379), TFAM (sc-166965), SOD2 (sc-133134), PINK1 (sc-517353), and PRKN (sc-32282) were from Santa Cruz (Dallas, TX, USA); Acetylated-Lysine Antibody (#9441, CST, Danvers, MA, USA); SIRT1 (13161-1-AP), SIRT3 (10099-1-AP), PGC1α (66369-1-Ig), and FOXO3a (66428-1-Ig) were from Proteintech (Wuhan, China). .. Ten-week-old Sprague-Dawley rats (weighing 280 g ± 30 g) were obtained from Changsheng Biotechnology (Liaoning, China, license number: SYXK 2019-0007).

Membrane:

Article Title: Knockdown of Ddx3x in mPFC induces autistic-like phenotype in mice via altered synaptic plasticity
Article Snippet: .. Proteins were then transferred to a polyvinylidene fluoride (PVDF) membrane (Millipore, No. IPVH00010, USA) and blocked with 5% non-fat milk in tris-buffered saline (TBS: 100 mM Tris, and 1.5 M NaCl, pH 7.6) containing 0.4% (v/v) tween 20 (TBST) for 1 h. The membrane was incubated overnight at 4 °C with primary antibodies against DDX3X (Abcam, No. ab271002, 1:2000, UK), MAPK1 (Abclonal, No. A0229, 1:1000, China), CAMKII-delta (Proteintech, No. 20667-1-AP, 1:1000, China), NMDAR (Huabio, No. JM11-26, 1:600, China), PSD95 (Proteintech, No. 20665-1-AP, 1:1000), PRRT1 (Proteintech, No. 66031-1-lg, 1:600), GAPDH (Abmart, No. M20050 , 1:5000, China), TUBA (Proteintech, No. 66031-1-lg, 1:10000), and VCL (Proteintech, No. 66305-1-lg, 1:15000). .. Secondary antibodies included anti-rabbit (Beyotime, No. A0208, 1:1000, China) and anti-mouse (Abmart, No. M21001 , 1:5000, China), incubated for 1 h at room temperature.

Article Title: Knockdown of Ddx3x in mPFC induces autistic-like phenotype in mice via altered synaptic plasticity.
Article Snippet: .. Proteins were then transferred to a polyvinylidene fluoride (PVDF) membrane (Millipore, No. IPVH00010, USA) and blocked with 5% non-fat milk in tris-buffered saline (TBS: 100mM Tris, and 1.5 M NaCl, pH 7.6) containing 0.4% (v/v) tween 20 (TBST) for 1 h. The membrane was incubated overnight at 4 °C with primary antibodies against DDX3X (Abcam, No. ab271002, 1:2000, UK), MAPK1 (Abclonal, No. A0229, 1:1000, China), CAMKII-delta (Proteintech, No. 20667-1-AP, 1:1000, China), NMDAR (Huabio, No. JM11-26, 1:600, China), PSD95 (Proteintech, No. 20665-1-AP, 1:1000), PRRT1 (Proteintech, No. 66031-1-lg, 1:600), GAPDH (Abmart, No. M20050, 1:5000, China), TUBA (Proteintech, No. 66031-1-lg, 1:10000), and Translational Psychiatry (2026) 16:216 VCL (Proteintech, No. 66305-1-lg, 1:15000). .. Secondary antibodies included anti-rabbit (Beyotime, No. A0208, 1:1000, China) and anti-mouse (Abmart, No. M21001, 1:5000, China), incubated for 1 h at room temperature.

Saline:

Article Title: Knockdown of Ddx3x in mPFC induces autistic-like phenotype in mice via altered synaptic plasticity
Article Snippet: .. Proteins were then transferred to a polyvinylidene fluoride (PVDF) membrane (Millipore, No. IPVH00010, USA) and blocked with 5% non-fat milk in tris-buffered saline (TBS: 100 mM Tris, and 1.5 M NaCl, pH 7.6) containing 0.4% (v/v) tween 20 (TBST) for 1 h. The membrane was incubated overnight at 4 °C with primary antibodies against DDX3X (Abcam, No. ab271002, 1:2000, UK), MAPK1 (Abclonal, No. A0229, 1:1000, China), CAMKII-delta (Proteintech, No. 20667-1-AP, 1:1000, China), NMDAR (Huabio, No. JM11-26, 1:600, China), PSD95 (Proteintech, No. 20665-1-AP, 1:1000), PRRT1 (Proteintech, No. 66031-1-lg, 1:600), GAPDH (Abmart, No. M20050 , 1:5000, China), TUBA (Proteintech, No. 66031-1-lg, 1:10000), and VCL (Proteintech, No. 66305-1-lg, 1:15000). .. Secondary antibodies included anti-rabbit (Beyotime, No. A0208, 1:1000, China) and anti-mouse (Abmart, No. M21001 , 1:5000, China), incubated for 1 h at room temperature.

Article Title: Knockdown of Ddx3x in mPFC induces autistic-like phenotype in mice via altered synaptic plasticity.
Article Snippet: .. Proteins were then transferred to a polyvinylidene fluoride (PVDF) membrane (Millipore, No. IPVH00010, USA) and blocked with 5% non-fat milk in tris-buffered saline (TBS: 100mM Tris, and 1.5 M NaCl, pH 7.6) containing 0.4% (v/v) tween 20 (TBST) for 1 h. The membrane was incubated overnight at 4 °C with primary antibodies against DDX3X (Abcam, No. ab271002, 1:2000, UK), MAPK1 (Abclonal, No. A0229, 1:1000, China), CAMKII-delta (Proteintech, No. 20667-1-AP, 1:1000, China), NMDAR (Huabio, No. JM11-26, 1:600, China), PSD95 (Proteintech, No. 20665-1-AP, 1:1000), PRRT1 (Proteintech, No. 66031-1-lg, 1:600), GAPDH (Abmart, No. M20050, 1:5000, China), TUBA (Proteintech, No. 66031-1-lg, 1:10000), and Translational Psychiatry (2026) 16:216 VCL (Proteintech, No. 66305-1-lg, 1:15000). .. Secondary antibodies included anti-rabbit (Beyotime, No. A0208, 1:1000, China) and anti-mouse (Abmart, No. M21001, 1:5000, China), incubated for 1 h at room temperature.



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RB@LCP-AR diminishes neuroinflammation and restores neuronal synapses in AD mice. AD mice were intravenously injected with the indicated formulations 5 times, then the brain tissues were collected for further analysis. (A–B) The levels of inflammatory TNF-α (A) and IL-6 (B). (C–D) The levels of MDA (C) and SOD (D). (E–G) The distribution and quantitative analysis of Iba-1 (astrocytes) and GFAP (microglia) in the brain tissues. Scale bar = 400 μm. (H–J) The distribution and quantitative analysis of PSD95 (postsynaptic) and synaptophysin (presynaptic) in the brain tissues. Scale bar = 400 μm. Data are presented by mean ± SEM (n = 6). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Journal: Materials Today Bio

Article Title: An anti-inflammatory neuroenhancer mitigates amyloid-β pathology to improve Alzheimer's disease therapy

doi: 10.1016/j.mtbio.2026.102874

Figure Lengend Snippet: RB@LCP-AR diminishes neuroinflammation and restores neuronal synapses in AD mice. AD mice were intravenously injected with the indicated formulations 5 times, then the brain tissues were collected for further analysis. (A–B) The levels of inflammatory TNF-α (A) and IL-6 (B). (C–D) The levels of MDA (C) and SOD (D). (E–G) The distribution and quantitative analysis of Iba-1 (astrocytes) and GFAP (microglia) in the brain tissues. Scale bar = 400 μm. (H–J) The distribution and quantitative analysis of PSD95 (postsynaptic) and synaptophysin (presynaptic) in the brain tissues. Scale bar = 400 μm. Data are presented by mean ± SEM (n = 6). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: Next, the sections were incubated with HRP-conjugated goat anti-mouse/rabbit IgG polymer for 20 min. To detect the synaptic protein, the sections were incubated with the primary antibody against synaptophysin and PSD95, and then incubated with corresponding secondary antibodies conjugated to Alexa Fluor 488 (Santa Cruz Biotechnology, USA) or Alexa Fluor 594 (Santa Cruz Biotechnology, USA), respectively.

Techniques: Injection