prrt1 (Proteintech)
Structured Review
Prrt1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 442 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/psd95/PSD95-Specific%2CDLG4+Antibody/pm41912502-131-82-83
Average 96 stars, based on 442 article reviews
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Electrophoresis:Article Title: D-serine ameliorates cognitive deficits by preserving neuronal and synaptic function in experimental anti-NMDAR encephalitis. Article Snippet: .. After electrophoresis and transfer, membranes were blocked in 5% skim milk prepared in PBST (0.05% Tween-20 in PBS) at room temperature (RT) and incubated overnight at 4 °C with primary antibodies against NMDAR1 (rabbit, 1:1000, AF6406, Affinity Biosciences, RRID: AB_2835236), Incubation:Article Title: D-serine ameliorates cognitive deficits by preserving neuronal and synaptic function in experimental anti-NMDAR encephalitis. Article Snippet: .. After electrophoresis and transfer, membranes were blocked in 5% skim milk prepared in PBST (0.05% Tween-20 in PBS) at room temperature (RT) and incubated overnight at 4 °C with primary antibodies against NMDAR1 (rabbit, 1:1000, AF6406, Affinity Biosciences, RRID: AB_2835236), Article Title: Knockdown of Ddx3x in mPFC induces autistic-like phenotype in mice via altered synaptic plasticity Article Snippet: .. Proteins were then transferred to a polyvinylidene fluoride (PVDF) membrane (Millipore, No. IPVH00010, USA) and blocked with 5% non-fat milk in tris-buffered saline (TBS: 100 mM Tris, and 1.5 M NaCl, pH 7.6) containing 0.4% (v/v) tween 20 (TBST) for 1 h. The membrane was incubated overnight at 4 °C with primary antibodies against DDX3X (Abcam, No. ab271002, 1:2000, UK), MAPK1 (Abclonal, No. A0229, 1:1000, China), CAMKII-delta (Proteintech, No. 20667-1-AP, 1:1000, China), NMDAR (Huabio, No. JM11-26, 1:600, China), Article Title: Knockdown of Ddx3x in mPFC induces autistic-like phenotype in mice via altered synaptic plasticity. Article Snippet: .. Proteins were then transferred to a polyvinylidene fluoride (PVDF) membrane (Millipore, No. IPVH00010, USA) and blocked with 5% non-fat milk in tris-buffered saline (TBS: 100mM Tris, and 1.5 M NaCl, pH 7.6) containing 0.4% (v/v) tween 20 (TBST) for 1 h. The membrane was incubated overnight at 4 °C with primary antibodies against DDX3X (Abcam, No. ab271002, 1:2000, UK), MAPK1 (Abclonal, No. A0229, 1:1000, China), CAMKII-delta (Proteintech, No. 20667-1-AP, 1:1000, China), NMDAR (Huabio, No. JM11-26, 1:600, China), Article Title: Penumbra-derived small extracellular vesicles carry pathogenic RNA driving remote damage after cortical infarction. Article Snippet: 7 Small extracellular vesicles (sEVs) efficiently transport pathological RNAs in ischemic stroke, 8 yet whether penumbral hypoxia-induced EVs deliver pathogenic cargo to remote regions, 9 impacting acute ischemic stroke outcomes, remains unclear.. 10 Using a focal ischemia mouse model, we isolated brain-derived EVs (BDEVs) from the 11 cortical penumbra and determined their pathological impact on synaptically connected 12 remote regions.. In vitro, penumbra BDEVs (PEVs) exacerbated recipient neuronal damage, 13 intensifying apoptosis and dendritic injury during oxygen-glucose deprivation/reoxygenation. other:Article Title: HMGCS2-dependent β-OHB/H3K9bhb ameliorates synaptic plasticity and cognition in Alzheimer’s disease Article Snippet: PSD95 , PSD95 , Poly- , 1:1000 , Western Blot:Article Title: Melatonin Ameliorates decaBDE-Induced Autism-Relevant Behaviors Through Promoting SIRT1/SIRT3/FOXO3a-Dependent Mitochondrial Quality Control. Article Snippet: Decabromodiphenyl ether (deca-BDE or BDE-209, 98%, CAS#1163-19-5) was obtained from J & K Chemical Technology (Beijing, China); sodium valproate (VPA, powder, 98%, CAS#1069-66-5) and melatonin (MT, powder, ≥98%, CAS#73-31-4) were from SigmaAldrich Trading Co., Ltd. (Shanghai, China); Sirt1/Sir2 inhibitor Selisistat (EX527, 99.85%, CAS#49843-98-3) was from MedChemExpress (Shanghai, China). .. Primary antibodies used for Western blotting were as follows: GAPDH (60004-1-Ig) and Article Title: Melatonin Ameliorates decaBDE-Induced Autism-Relevant Behaviors Through Promoting SIRT1/SIRT3/FOXO3a-Dependent Mitochondrial Quality Control Article Snippet: Decabromodiphenyl ether (deca-BDE or BDE-209, 98%, CAS#1163-19-5) was obtained from J & K Chemical Technology (Beijing, China); sodium valproate (VPA, powder, 98%, CAS#1069-66-5) and melatonin (MT, powder, ≥98%, CAS#73-31-4) were from Sigma-Aldrich Trading Co., Ltd. (Shanghai, China); Sirt1/Sir2 inhibitor Selisistat (EX527, 99.85%, CAS#49843-98-3) was from MedChemExpress (Shanghai, China). .. Primary antibodies used for Western blotting were as follows: GAPDH (60004-1-Ig) and Membrane:Article Title: Knockdown of Ddx3x in mPFC induces autistic-like phenotype in mice via altered synaptic plasticity Article Snippet: .. Proteins were then transferred to a polyvinylidene fluoride (PVDF) membrane (Millipore, No. IPVH00010, USA) and blocked with 5% non-fat milk in tris-buffered saline (TBS: 100 mM Tris, and 1.5 M NaCl, pH 7.6) containing 0.4% (v/v) tween 20 (TBST) for 1 h. The membrane was incubated overnight at 4 °C with primary antibodies against DDX3X (Abcam, No. ab271002, 1:2000, UK), MAPK1 (Abclonal, No. A0229, 1:1000, China), CAMKII-delta (Proteintech, No. 20667-1-AP, 1:1000, China), NMDAR (Huabio, No. JM11-26, 1:600, China), Article Title: Knockdown of Ddx3x in mPFC induces autistic-like phenotype in mice via altered synaptic plasticity. Article Snippet: .. Proteins were then transferred to a polyvinylidene fluoride (PVDF) membrane (Millipore, No. IPVH00010, USA) and blocked with 5% non-fat milk in tris-buffered saline (TBS: 100mM Tris, and 1.5 M NaCl, pH 7.6) containing 0.4% (v/v) tween 20 (TBST) for 1 h. The membrane was incubated overnight at 4 °C with primary antibodies against DDX3X (Abcam, No. ab271002, 1:2000, UK), MAPK1 (Abclonal, No. A0229, 1:1000, China), CAMKII-delta (Proteintech, No. 20667-1-AP, 1:1000, China), NMDAR (Huabio, No. JM11-26, 1:600, China), Saline:Article Title: Knockdown of Ddx3x in mPFC induces autistic-like phenotype in mice via altered synaptic plasticity Article Snippet: .. Proteins were then transferred to a polyvinylidene fluoride (PVDF) membrane (Millipore, No. IPVH00010, USA) and blocked with 5% non-fat milk in tris-buffered saline (TBS: 100 mM Tris, and 1.5 M NaCl, pH 7.6) containing 0.4% (v/v) tween 20 (TBST) for 1 h. The membrane was incubated overnight at 4 °C with primary antibodies against DDX3X (Abcam, No. ab271002, 1:2000, UK), MAPK1 (Abclonal, No. A0229, 1:1000, China), CAMKII-delta (Proteintech, No. 20667-1-AP, 1:1000, China), NMDAR (Huabio, No. JM11-26, 1:600, China), Article Title: Knockdown of Ddx3x in mPFC induces autistic-like phenotype in mice via altered synaptic plasticity. Article Snippet: .. Proteins were then transferred to a polyvinylidene fluoride (PVDF) membrane (Millipore, No. IPVH00010, USA) and blocked with 5% non-fat milk in tris-buffered saline (TBS: 100mM Tris, and 1.5 M NaCl, pH 7.6) containing 0.4% (v/v) tween 20 (TBST) for 1 h. The membrane was incubated overnight at 4 °C with primary antibodies against DDX3X (Abcam, No. ab271002, 1:2000, UK), MAPK1 (Abclonal, No. A0229, 1:1000, China), CAMKII-delta (Proteintech, No. 20667-1-AP, 1:1000, China), NMDAR (Huabio, No. JM11-26, 1:600, China), |
