psc833 (Novartis)
86
Structured Review
Novartis
psc833
Psc833, supplied by Novartis, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/psc833/psc833/pm40362589-408-0-6
Average 86 stars, based on 1 article reviews
Psc833, supplied by Novartis, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/psc833/psc833/pm40362589-408-0-6
Average 86 stars, based on 1 article reviews
psc833 - by Bioz Stars,
2026-09
86/100 stars
Images
1) Product Images from "N-Acetylcysteine Attenuates Aβ-Mediated Oxidative Stress, Blood-Brain Barrier Leakage, and Renal Dysfunction in 5xFAD Mice."
Article Title: N-Acetylcysteine Attenuates Aβ-Mediated Oxidative Stress, Blood-Brain Barrier Leakage, and Renal Dysfunction in 5xFAD Mice.
Journal: International journal of molecular sciences
doi: 10.3390/ijms26094352
Figure Legend Snippet: Figure 3. Effect of NAC on blood–brain barrier integrity in 5xFAD and WT mice. (A) Brain capillary 4-HNE levels (µg/mg protein) in untreated 5xFAD mice compared to those in untreated WT mice (p = 0.0018) or NAC-treated 5xFAD mice (p = 0.0333). Circles represent technical replicates. Data are presented as mean (filled columns) ± standard error of the mean (SEM). (B) Isolated capillaries were incubated with 2 µM NBD-CSA, a fluorescent P-glycoprotein-specific substrate, for 1 h alone or with PSC833. Specific luminal NBD-CSA fluorescence was taken as the difference between total luminal fluorescence and fluorescence in the presence of the P-gp inhibitor PSC833. Data are mean ± SEM for 10 capillaries from one preparation (pooled tissue from 15 mice per group), in arbitrary fluorescence units (scale 0–255). **, significantly lower than control, p < 0.01. (C) Plasma S100β levels (pg/mL) were 2.5-fold higher (p < 0.0001) for untreated 5xFAD mice (red column; n = 15 mice) than for untreated WT mice (blue column; n = 15 mice). NAC treatment (green column; n = 15 mice) significantly lowered S100β levels in 5xFAD mice (p < 0.0001). Circles represent biological replicates. (D) Texas Red leakage from capillary lumens was imaged over time using a confocal microscope for untreated WT mice (blue line), untreated 5xFAD mice (red line), and NAC-treated 5xFAD mice (green line). 100 mM mannitol served as a positive control for barrier opening (black line). Data are mean ± SEM for 7 brain capillaries per time point from one capillary isolation per group from 15 mice. Shown are arbitrary units (0–255). First-order efflux rates were calculated using non-linear regression.
Techniques Used: Isolation, Incubation, Fluorescence, Control, Clinical Proteomics, Microscopy, Positive Control
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These efflux systems affect not only rates of oral absorption but also rates of excretion through the liver, blood–brain barrier, and accumulation in potential target cells that upregulate efflux systems.. Current methods to determine drugs’ P-gp transport potential include in vitro bidirectional transport studies, and the two most common cell lines used are Caco2 and MDR1-transfected MDCK models. Article Title: A New ABCB1 Inhibitor Enhances the Anticancer Effect of Doxorubicin in Both In Vitro and In Vivo Models of NSCLC. Article Snippet: The ABCA1 and ABCG2 inhibitors, probucol and fumetrimorgin C, were purchased from Sigma Aldrich (St. Louis, MO, USA); the Article Title: N-Acetylcysteine Attenuates Aβ-Mediated Oxidative Stress, Blood-Brain Barrier Leakage, and Renal Dysfunction in 5xFAD Mice. Article Snippet: |
![Fig. 1. Generation of stable human kidney cell lines with proven origin. A: Schematic of the human kidney and the cortex illustrates the source of isolated cells. B: Immunofluorescence staining of PDGFRβ+, CD10+ and CD31+ specific antibodies for cells in cortex of human kidney. C: Enumeration of the workflow from isolating the cells to the generation of genetically tagged cells. D: Representative pictures of genetically labeled immortalized PDGFRβ+, proximal tubular epithelial and endothelial cell lines. 4′,6-diamidino-2-phenylindole (DAPI) was used for nuclear staining. E: Principal component analysis (PCA) plot of the generated three cell lines. The three cell lines show higher variability from each other, while technical replicates are similar to each other. F: Shared DE genes between isolated cell lines and human bulk RNA seq [13]. G: CD10+ (red) cells stained for cell-specific proximal tubule epithelial markers cubilin (green, left) and CD13 (green, right) including DAPI. H: Heatmap of bulk RNA-sequencing data demonstrating the genes expressed in CD10+. I: P-gp Calcein-AM transporter assay of CD10+ and negative control PDGFRβ+ cells, with or without transporter inhibitor <t>PSC833.</t> ***p < 0.001 (one-way ANOVA analysis followed by Tukey post-test), n = 3 with each 12 replicates. J: Presence of intracellular vWF (red) in CD31+ (green) cells including DAPI. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)](https://pub-med-unpaywalled-images-cdn.bioz.com/pub_med_ids_ending_with_5928/pm39705928/pm39705928__page3_image1.jpg)