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protac3  (Tocris)


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Bioz Manufacturer Symbol Tocris manufactures this product  
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    Tocris protac3
    Fig. 4. Degradation profile of wildtype EGFR in A549 cells. Cells were pre-treated as described in (Fig. 1) with EGF as RTK ligand and either <t>PROTAC3</t> (A,C,E,G,I) or Gefitinib (B,D,F,H,J) was added for 24 h. Cells were then analysed by immunoblotting (A,B) with quantification (C,D), immunofluorescence for the whole cell (E,F) or plasma membrane only (G,H) or flow cytometry (I,J). Graphs show the mean SEM of at least 3 biological replicates. The dotted line indicates a value of 0.8. All details regarding statistical analyses and p-values are provided in the (Supplementary Table 3).
    Protac3, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protac3/product/Tocris
    Average 93 stars, based on 1 article reviews
    protac3 - by Bioz Stars, 2026-05
    93/100 stars

    Images

    1) Product Images from "Orthogonal validation of PROTAC mediated degradation of the integral membrane proteins EGFR and c-MET."

    Article Title: Orthogonal validation of PROTAC mediated degradation of the integral membrane proteins EGFR and c-MET.

    Journal: Scientific reports

    doi: 10.1038/s41598-024-84217-2

    Fig. 4. Degradation profile of wildtype EGFR in A549 cells. Cells were pre-treated as described in (Fig. 1) with EGF as RTK ligand and either PROTAC3 (A,C,E,G,I) or Gefitinib (B,D,F,H,J) was added for 24 h. Cells were then analysed by immunoblotting (A,B) with quantification (C,D), immunofluorescence for the whole cell (E,F) or plasma membrane only (G,H) or flow cytometry (I,J). Graphs show the mean SEM of at least 3 biological replicates. The dotted line indicates a value of 0.8. All details regarding statistical analyses and p-values are provided in the (Supplementary Table 3).
    Figure Legend Snippet: Fig. 4. Degradation profile of wildtype EGFR in A549 cells. Cells were pre-treated as described in (Fig. 1) with EGF as RTK ligand and either PROTAC3 (A,C,E,G,I) or Gefitinib (B,D,F,H,J) was added for 24 h. Cells were then analysed by immunoblotting (A,B) with quantification (C,D), immunofluorescence for the whole cell (E,F) or plasma membrane only (G,H) or flow cytometry (I,J). Graphs show the mean SEM of at least 3 biological replicates. The dotted line indicates a value of 0.8. All details regarding statistical analyses and p-values are provided in the (Supplementary Table 3).

    Techniques Used: Western Blot, Immunofluorescence, Clinical Proteomics, Membrane, Flow Cytometry

    Fig. 5. Degradation profile of ex19del EGFR in HCC827 cells. Cells were pre-treated as described in (Fig. 1) with EGF as RTK ligand and either PROTAC3 (A,C,E,G,I) or Gefitinib (B,D,F,H,J) was added for 24 h. Cells were then analysed by immunoblotting (A,B) with quantification (C,D), immunofluorescence for the whole cell (E,F) or plasma membrane only (G,H) or flow cytometry (I,J). Graphs show the mean SEM of at least 3 biological replicates. The dotted line indicates a value of 0.8. All details regarding statistical analyses and p-values are provided in the (Supplementary Table 4).
    Figure Legend Snippet: Fig. 5. Degradation profile of ex19del EGFR in HCC827 cells. Cells were pre-treated as described in (Fig. 1) with EGF as RTK ligand and either PROTAC3 (A,C,E,G,I) or Gefitinib (B,D,F,H,J) was added for 24 h. Cells were then analysed by immunoblotting (A,B) with quantification (C,D), immunofluorescence for the whole cell (E,F) or plasma membrane only (G,H) or flow cytometry (I,J). Graphs show the mean SEM of at least 3 biological replicates. The dotted line indicates a value of 0.8. All details regarding statistical analyses and p-values are provided in the (Supplementary Table 4).

    Techniques Used: Western Blot, Immunofluorescence, Clinical Proteomics, Membrane, Flow Cytometry



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    Fig. 4. Degradation profile of wildtype EGFR in A549 cells. Cells were pre-treated as described in (Fig. 1) with EGF as RTK ligand and either <t>PROTAC3</t> (A,C,E,G,I) or Gefitinib (B,D,F,H,J) was added for 24 h. Cells were then analysed by immunoblotting (A,B) with quantification (C,D), immunofluorescence for the whole cell (E,F) or plasma membrane only (G,H) or flow cytometry (I,J). Graphs show the mean SEM of at least 3 biological replicates. The dotted line indicates a value of 0.8. All details regarding statistical analyses and p-values are provided in the (Supplementary Table 3).
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    Image Search Results


    Fig. 4. Degradation profile of wildtype EGFR in A549 cells. Cells were pre-treated as described in (Fig. 1) with EGF as RTK ligand and either PROTAC3 (A,C,E,G,I) or Gefitinib (B,D,F,H,J) was added for 24 h. Cells were then analysed by immunoblotting (A,B) with quantification (C,D), immunofluorescence for the whole cell (E,F) or plasma membrane only (G,H) or flow cytometry (I,J). Graphs show the mean SEM of at least 3 biological replicates. The dotted line indicates a value of 0.8. All details regarding statistical analyses and p-values are provided in the (Supplementary Table 3).

    Journal: Scientific reports

    Article Title: Orthogonal validation of PROTAC mediated degradation of the integral membrane proteins EGFR and c-MET.

    doi: 10.1038/s41598-024-84217-2

    Figure Lengend Snippet: Fig. 4. Degradation profile of wildtype EGFR in A549 cells. Cells were pre-treated as described in (Fig. 1) with EGF as RTK ligand and either PROTAC3 (A,C,E,G,I) or Gefitinib (B,D,F,H,J) was added for 24 h. Cells were then analysed by immunoblotting (A,B) with quantification (C,D), immunofluorescence for the whole cell (E,F) or plasma membrane only (G,H) or flow cytometry (I,J). Graphs show the mean SEM of at least 3 biological replicates. The dotted line indicates a value of 0.8. All details regarding statistical analyses and p-values are provided in the (Supplementary Table 3).

    Article Snippet: For starvation experiments, cells were washed twice in PBS and grown in respective growth media without FBS for 6–8 h. Subsequently media were exchanged for full growth media and cells were treated with SJF8240 (Tocris, 7266), Foretinib (Stratech, ORB322222), PROTAC3 (Tocris, 7258) or Gefitinib (AstraZeneca).

    Techniques: Western Blot, Immunofluorescence, Clinical Proteomics, Membrane, Flow Cytometry

    Fig. 5. Degradation profile of ex19del EGFR in HCC827 cells. Cells were pre-treated as described in (Fig. 1) with EGF as RTK ligand and either PROTAC3 (A,C,E,G,I) or Gefitinib (B,D,F,H,J) was added for 24 h. Cells were then analysed by immunoblotting (A,B) with quantification (C,D), immunofluorescence for the whole cell (E,F) or plasma membrane only (G,H) or flow cytometry (I,J). Graphs show the mean SEM of at least 3 biological replicates. The dotted line indicates a value of 0.8. All details regarding statistical analyses and p-values are provided in the (Supplementary Table 4).

    Journal: Scientific reports

    Article Title: Orthogonal validation of PROTAC mediated degradation of the integral membrane proteins EGFR and c-MET.

    doi: 10.1038/s41598-024-84217-2

    Figure Lengend Snippet: Fig. 5. Degradation profile of ex19del EGFR in HCC827 cells. Cells were pre-treated as described in (Fig. 1) with EGF as RTK ligand and either PROTAC3 (A,C,E,G,I) or Gefitinib (B,D,F,H,J) was added for 24 h. Cells were then analysed by immunoblotting (A,B) with quantification (C,D), immunofluorescence for the whole cell (E,F) or plasma membrane only (G,H) or flow cytometry (I,J). Graphs show the mean SEM of at least 3 biological replicates. The dotted line indicates a value of 0.8. All details regarding statistical analyses and p-values are provided in the (Supplementary Table 4).

    Article Snippet: For starvation experiments, cells were washed twice in PBS and grown in respective growth media without FBS for 6–8 h. Subsequently media were exchanged for full growth media and cells were treated with SJF8240 (Tocris, 7266), Foretinib (Stratech, ORB322222), PROTAC3 (Tocris, 7258) or Gefitinib (AstraZeneca).

    Techniques: Western Blot, Immunofluorescence, Clinical Proteomics, Membrane, Flow Cytometry