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primepcrtm primers  (Bio-Rad)


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    Structured Review

    Bio-Rad primepcrtm primers
    Primepcrtm Primers, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 217 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primepcr/PrimePCR+Analysis+Software/pmc12832818-147-4-8
    Average 99 stars, based on 217 article reviews
    primepcrtm primers - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    other:

    Article Title: Hormonal regulation of Semaphorin 7a in ER+ breast cancer drives therapeutic resistance
    Article Snippet: cDNA was quantified via qPCR using Bio-Rad iTaq Universal SYBR Green Supermix and primers for SEMA7A (Bio-Rad PrimePCR, Hercules, CA) using the following conditions: 95°C for 3 min, then 40 cycles of 95°C for 15 sec and 60°C for 1 min, 95°C for 30 sec, and 55°C for 1 min. Primer fidelity was assessed for each qPCR experiment using a 95°C melt curve.

    Expressing:

    Article Title: Methylation of estrogen receptor 2 ( ESR2 ) in deep paravertebral muscles and its association with idiopathic scoliosis
    Article Snippet: .. Quantitative analysis of ESR2 mRNA expression was evaluated using a hydrolysis probe PrimePCR (qHsaCEP0052206, BioRad). .. The hypoxanthine–guanine phosphoribosyltransferase ( HPRT ) gene was used as a reference gene (RealTime ready HPRT , 102079, Roche).

    Gene Expression:

    Article Title: miRNA Regulation of NK Cells Antiviral Response in Children With Severe and/or Recurrent Herpes Simplex Virus Infections
    Article Snippet: Total RNA was extracted from cell suspension using GenEluteTM Total RNA Purification Kit (Sigma Aldrich), then cDNA was generated with the iScript Advanced cDNA synthesis kit (Biorad, Carlsbad CA). .. For antiviral response gene expression analysis, the PrimePCR predesigned plates (384-well format, Bio-Rad) were used, altogether with SsoAdvanced SYBR Green supermix (Bio-Rad), according to manufacturer’s protocol. ..

    SYBR Green Assay:

    Article Title: miRNA Regulation of NK Cells Antiviral Response in Children With Severe and/or Recurrent Herpes Simplex Virus Infections
    Article Snippet: Total RNA was extracted from cell suspension using GenEluteTM Total RNA Purification Kit (Sigma Aldrich), then cDNA was generated with the iScript Advanced cDNA synthesis kit (Biorad, Carlsbad CA). .. For antiviral response gene expression analysis, the PrimePCR predesigned plates (384-well format, Bio-Rad) were used, altogether with SsoAdvanced SYBR Green supermix (Bio-Rad), according to manufacturer’s protocol. ..

    Mutagenesis:

    Article Title: A Phase Ib Study of Onvansertib, a Novel Oral PLK1 Inhibitor, in Combination Therapy for Patients with Relapsed or Refractory Acute Myeloid Leukemia
    Article Snippet: Data analysis was performed using Archer Analysis version 6.0.3.2 (ArcherDX) with custom targets, VariantPlex_Myeloid_GSP5031-v1.0, and targeted mutations, Archer Comprehensive Targets v1.1. .. For 20 patients, a driver mutation was identified by targeted NGS, and probe-based assays enabling detection of variant and wild-type alleles were commercially obtained (PrimePCR, Bio-Rad Laboratories, or TaqMan SNP Genotyping Assays, Thermo Fisher Scientific). .. Digital droplet PCR (ddPCR) was performed using the Bio-Rad QX200 Droplet Digital PCR System following the manufacturer’s protocols.

    Next-Generation Sequencing:

    Article Title: A Phase Ib Study of Onvansertib, a Novel Oral PLK1 Inhibitor, in Combination Therapy for Patients with Relapsed or Refractory Acute Myeloid Leukemia
    Article Snippet: Data analysis was performed using Archer Analysis version 6.0.3.2 (ArcherDX) with custom targets, VariantPlex_Myeloid_GSP5031-v1.0, and targeted mutations, Archer Comprehensive Targets v1.1. .. For 20 patients, a driver mutation was identified by targeted NGS, and probe-based assays enabling detection of variant and wild-type alleles were commercially obtained (PrimePCR, Bio-Rad Laboratories, or TaqMan SNP Genotyping Assays, Thermo Fisher Scientific). .. Digital droplet PCR (ddPCR) was performed using the Bio-Rad QX200 Droplet Digital PCR System following the manufacturer’s protocols.

    Variant Assay:

    Article Title: A Phase Ib Study of Onvansertib, a Novel Oral PLK1 Inhibitor, in Combination Therapy for Patients with Relapsed or Refractory Acute Myeloid Leukemia
    Article Snippet: Data analysis was performed using Archer Analysis version 6.0.3.2 (ArcherDX) with custom targets, VariantPlex_Myeloid_GSP5031-v1.0, and targeted mutations, Archer Comprehensive Targets v1.1. .. For 20 patients, a driver mutation was identified by targeted NGS, and probe-based assays enabling detection of variant and wild-type alleles were commercially obtained (PrimePCR, Bio-Rad Laboratories, or TaqMan SNP Genotyping Assays, Thermo Fisher Scientific). .. Digital droplet PCR (ddPCR) was performed using the Bio-Rad QX200 Droplet Digital PCR System following the manufacturer’s protocols.



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