Journal: APL Bioengineering

Article Title: Integrating Mendelian randomization and multi-omics analysis unravels gut microbiota-driven metabolic mechanisms in sepsis and identifies diagnostic biomarkers through experimental validation

doi: 10.1063/5.0296018

Figure Lengend Snippet: Diagnostic value of key diagnostic genes. (a) ROC curves illustrating the diagnostic performance of the three key diagnostic genes. (b)–(d) Expression levels of ABCC1 , CYP1B1 , and PPARG in dataset GSE134364 . (e)–(g) Expression levels of ABCC1 , CYP1B1 , and PPARG in dataset GSE65682 . ROC: receiver operating characteristic curve; AUC: area under the ROC curve.

Article Snippet: The membrane was blocked and incubated overnight at 4 °C with primary antibodies: ABCC1 (A2223, Abclonal, 1:1000), CYP1B1 (A1377, Abclonal, 1:1000), and PPARG (16643-1-AP, Proteintech, 1:1000).

Techniques: Diagnostic Assay, Expressing

Journal: APL Bioengineering

Article Title: Integrating Mendelian randomization and multi-omics analysis unravels gut microbiota-driven metabolic mechanisms in sepsis and identifies diagnostic biomarkers through experimental validation

doi: 10.1063/5.0296018

Figure Lengend Snippet: Expression of key diagnostic genes in human alveolar organoids derived from pulmonary sepsis patients. (a) Representative bright-field image of human alveolar organoids cultured for 7 days; scale bar = 200 μ m. (b) HE staining of human alveolar organoids. (c) Immunofluorescence quantification of AQP5, SOX9, and SPC in human alveolar organoids. (d) WB analysis showing the expression levels of ABCC1, CYP1B1, and PPARG in human alveolar organoids.

Article Snippet: The membrane was blocked and incubated overnight at 4 °C with primary antibodies: ABCC1 (A2223, Abclonal, 1:1000), CYP1B1 (A1377, Abclonal, 1:1000), and PPARG (16643-1-AP, Proteintech, 1:1000).

Techniques: Expressing, Diagnostic Assay, Derivative Assay, Cell Culture, Staining, Immunofluorescence

Journal: APL Bioengineering

Article Title: Integrating Mendelian randomization and multi-omics analysis unravels gut microbiota-driven metabolic mechanisms in sepsis and identifies diagnostic biomarkers through experimental validation

doi: 10.1063/5.0296018

Figure Lengend Snippet: Expression of key diagnostic genes in bronchial organoids derived from pulmonary sepsis rats. (a) Representative bright-field image of rat bronchial organoids cultured for 7 days; scale bar = 200 μ m. (b) HE staining and IHC results for Ki67, P63, FOXJ1, MUC5AC, and CC10 in rat bronchial organoids. (c) Morphological status of rat bronchial organoids infected with four bacterial concentrations (MOI = 0, 5, 10, and 20) of Escherichia coli and Staphylococcus aureus at 0, 6, 12, and 24 h. (d) Fluorescence detection of organoid viability in rat bronchial organoids infected with MOI = 20 bacteria ( E. coli and S. aureus ) at 12 and 24 h. (e) RT-qPCR analysis quantifying the expression levels of ABCC1 , CYP1B1 , and PPARG in rat bronchial organoids.

Article Snippet: The membrane was blocked and incubated overnight at 4 °C with primary antibodies: ABCC1 (A2223, Abclonal, 1:1000), CYP1B1 (A1377, Abclonal, 1:1000), and PPARG (16643-1-AP, Proteintech, 1:1000).

Techniques: Expressing, Diagnostic Assay, Derivative Assay, Cell Culture, Staining, Infection, Fluorescence, Bacteria, Quantitative RT-PCR

Journal: APL Bioengineering

Article Title: Integrating Mendelian randomization and multi-omics analysis unravels gut microbiota-driven metabolic mechanisms in sepsis and identifies diagnostic biomarkers through experimental validation

doi: 10.1063/5.0296018

Figure Lengend Snippet: GSEA, immune infiltration, and GSVA analyses of key diagnostic genes. (a)–(c) GSEA analysis of ABCC1 , CYP1B1 , and PPARG , identifying enriched pathways associated with these genes. (d) Differences in immune infiltrating cell populations between healthy donors and sepsis patients. (e) Correlation analysis between ABCC1 , CYP1B1 , PPARG , and immune cells. (f) and (g) GSVA analysis of ABCC1 , CYP1B1 , and PPARG.

Article Snippet: The membrane was blocked and incubated overnight at 4 °C with primary antibodies: ABCC1 (A2223, Abclonal, 1:1000), CYP1B1 (A1377, Abclonal, 1:1000), and PPARG (16643-1-AP, Proteintech, 1:1000).

Techniques: Diagnostic Assay

Journal: APL Bioengineering

Article Title: Integrating Mendelian randomization and multi-omics analysis unravels gut microbiota-driven metabolic mechanisms in sepsis and identifies diagnostic biomarkers through experimental validation

doi: 10.1063/5.0296018

Figure Lengend Snippet: Single-cell analysis of ABCC1 , CYP1B1 , and PPARG . (a) and (b) Expression of ABCC1 , CYP1B1 , and PPARG in different immune cells.

Article Snippet: The membrane was blocked and incubated overnight at 4 °C with primary antibodies: ABCC1 (A2223, Abclonal, 1:1000), CYP1B1 (A1377, Abclonal, 1:1000), and PPARG (16643-1-AP, Proteintech, 1:1000).

Techniques: Single-cell Analysis, Expressing

Journal: Communications Biology

Article Title: Environmentally relevant lanthanum chloride exposure induces hepatic steatosis in zebrafish larvae via PPAR α -dependent ApoB suppression

doi: 10.1038/s42003-026-09697-6

Figure Lengend Snippet: a Volcano plot; b Heatmap of DEGs related to liver development and hepatic lipid metabolism; c KEGG analysis; d Circos diagram showing the relationship between key DEGs and KEGG pathways; e Correlation analysis between the key DEGs and TG-VLDL metabolism-related SDMs; f qPCR validation, n = 4–5/group; g Inhibitory effect of LaCl 3 on PPAR α -driven luciferase activity in HEK-293T cells. Different letters indicate statistically significant differences among groups ( p < 0.0001); n = 4–5/group; h Model of how PPAR α -mediated TG-VLDL biosynthesis inhibition. Mean ± S.E.M.

Article Snippet: For mechanism exploration, the same LaCl 3 exposure regimen (2–120 hpf) was co-treated with PPAR α agonist (GW7647, 1 μM; 99.45%; MCE, CAS: 265129-71-3, USA), OA (0.2 μM; 99%, Aladdin, CAS: 112-80-1, China), or MG132 (1 μM; 98%, Aladdin, CAS: 1211877-36-9, China).

Techniques: Biomarker Discovery, Luciferase, Activity Assay, Inhibition

Journal: Communications Biology

Article Title: Environmentally relevant lanthanum chloride exposure induces hepatic steatosis in zebrafish larvae via PPAR α -dependent ApoB suppression

doi: 10.1038/s42003-026-09697-6

Figure Lengend Snippet: a , b Representative images and quantitative analysis of liver fluorescence for each group (Control, LaCl 3 , and LaCl 3 + GW7647) at 120 hpf, n = 17–18/group; c Representative images of H&E staining in the liver sections. Red arrows indicate lipid droplets, black arrows indicate nuclear deformation, and orange arrows indicate vacuolization, n = 5/group. d , e Representative images and quantitative analysis of ORO staining in larvae, n = 15/group. The level of ( f ) TG, ( g ) VLDL, ( h ) FFA, ( i ) ALT, and ( j ) AST, n = 4–5/group. k Expression levels of PPAR α pathway-related genes for each group, n = 4–5/group; ** p < 0.01, *** p < 0.001, **** p < 0.0001, exact p values are provided in the Supplementary Data. l , m Western blot analysis of Ppara in zebrafish hepatic tissues, n = 4/group; n Schematic diagram of PPAR α in the regulation of LaCl 3 -induced TG-VLDL biosynthesis inhibition. Mean ± S.E.M.

Article Snippet: For mechanism exploration, the same LaCl 3 exposure regimen (2–120 hpf) was co-treated with PPAR α agonist (GW7647, 1 μM; 99.45%; MCE, CAS: 265129-71-3, USA), OA (0.2 μM; 99%, Aladdin, CAS: 112-80-1, China), or MG132 (1 μM; 98%, Aladdin, CAS: 1211877-36-9, China).

Techniques: Fluorescence, Control, Staining, Expressing, Western Blot, Inhibition