Journal: PLoS ONE

Article Title: Transcriptional Regulation of Flotillins by the Extracellularly Regulated Kinases and Retinoid X Receptor Complexes

doi: 10.1371/journal.pone.0045514

Figure Lengend Snippet: Oligonucleotides and plasmids.

Article Snippet: pSV Sport PPARγ , PPARγ expression plasmid , Addgene 8886, .

Techniques: Luciferase, Plasmid Preparation, Expressing, Control, Dominant Negative Mutation

Journal: PLoS ONE

Article Title: Transcriptional Regulation of Flotillins by the Extracellularly Regulated Kinases and Retinoid X Receptor Complexes

doi: 10.1371/journal.pone.0045514

Figure Lengend Snippet: Flotillin promoter constructs F1-1330 (A, C) or F2-2130 (B, D) were cotransfected into Hela cells together with expression plasmids for RAR, RXR, PPARγ or with empty PSV control plasmid. One day post-transfection, the cells were stimulated with trans-RA (1 µM) for 24 h in serum-free medium. Relative luciferase activity of the unstimulated control sample was set as 1. F1-1330 (E) and F2-2130 (F) transfected Hela cells were stimulated with troglitazone for 24 h in serum-free medium. Values are mean ± standard deviation of at least 3 experiments measured in duplicates. ***p<0.001; **p<0.01; *p<0.05 vs. respective control.

Article Snippet: pSV Sport PPARγ , PPARγ expression plasmid , Addgene 8886, .

Techniques: Construct, Expressing, Control, Plasmid Preparation, Transfection, Luciferase, Activity Assay, Standard Deviation

Journal: PLoS ONE

Article Title: Transcriptional Regulation of Flotillins by the Extracellularly Regulated Kinases and Retinoid X Receptor Complexes

doi: 10.1371/journal.pone.0045514

Figure Lengend Snippet: Hela cells were transiently transfected with expression constructs for RAR, RXR, or a combination of both. Empty PSV vector served as a control. One day post-transfection, the cells were stimulated with trans-RA (1 µM) in serum-free medium for 24 h. Cell lysates were analyzed for flotillin-1 (A), flotillin-2 (B), RAR and RXR (C) by Western blotting. D and E show a densitometric quantification of flotillin expression. F: Cells were transfected with RAR or PPARγ expression construct or empty PSV. RNA was isolated, transcribed into cDNA and flotillin mRNA was measured by qPCR. Values are mean ± standard deviation of at least 3 experiments. ###, p<0.001; #, p<0.05; vs control *, p<0.05 vs. unstimulated sample.

Article Snippet: pSV Sport PPARγ , PPARγ expression plasmid , Addgene 8886, .

Techniques: Transfection, Expressing, Construct, Plasmid Preparation, Control, Western Blot, Isolation, Standard Deviation

Journal: Journal of Animal and Feed Sciences

Article Title: β-carotene as a dietary factor affecting expression of genes connected with carotenoid, vitamin A and lipid metabolism in the subcutaneous and omental adipose tissue of beef cattle

doi: 10.22358/jafs/117866/2020

Figure Lengend Snippet: Figure 3. Expression of BCMO1 (A), PPARγ (B) and RXRα (C) protein in omental fat of beef cattle fed different doses of β-carotene (βC) (0, 600, 1200 and 1800 mg/d); Figure A, B and C: bars present means ± standard error (SE), for 3 steers per group; a–c – bars with different superscripts vary significantly (P < 0.05); Figure D: bands present one representative sample from each group

Article Snippet: The primary antibodies were chosen as follows: anti-BCMO1 polyclonal antibodies (1:200, sc-163736; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), anti-PPARγ polyclonal antibodies (1:400, NB120-19481; Novus Biologicals, Littleton, CO, USA), anti-RXRα monoclonal antibodies (1:1000, LS-C80051; LifeSpan BioSciences, Inc., Seattle, WA, USA), and anti-β-actin monoclonal antibodies (1:1000, 4970S; Cell Signaling Technology, Inc., Beverly, MA, USA).

Techniques: Expressing

Journal: Journal of Animal and Feed Sciences

Article Title: β-carotene as a dietary factor affecting expression of genes connected with carotenoid, vitamin A and lipid metabolism in the subcutaneous and omental adipose tissue of beef cattle

doi: 10.22358/jafs/117866/2020

Figure Lengend Snippet: Figure 2. Expression of BCMO1 (A), PPARγ (B) and RXRα (C) protein in subcutaneous fat of beef cattle fed different doses of β-carotene (βC) (0, 600, 1200 and 1800 mg/d); Figure A, B and C: bars present means ± standard error (SE), for 3 steers per group; a–b – bars with different superscripts vary significantly (P < 0.05); Figure D: bands present one representative sample from each group

Article Snippet: The primary antibodies were chosen as follows: anti-BCMO1 polyclonal antibodies (1:200, sc-163736; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), anti-PPARγ polyclonal antibodies (1:400, NB120-19481; Novus Biologicals, Littleton, CO, USA), anti-RXRα monoclonal antibodies (1:1000, LS-C80051; LifeSpan BioSciences, Inc., Seattle, WA, USA), and anti-β-actin monoclonal antibodies (1:1000, 4970S; Cell Signaling Technology, Inc., Beverly, MA, USA).

Techniques: Expressing