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pmd2g  (ATCC)


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    Structured Review

    ATCC pmd2g
    Pmd2g, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 38021 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pmd2g/293T/pmc13064391-54-8-5
    Average 99 stars, based on 38021 article reviews
    pmd2g - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Derivative Assay:

    Article Title: Generation of individualized immunocompatible endothelial cells from HLA-I-matched human pluripotent stem cells.
    Article Snippet: .. Materials and methods Cell lines, vectors and mice Human iPSC lines: LHPb-YaabC55 (C55), LHPbYaabC56 (C56) and LHPa-MaaaC10 (C10) were derived from human urine-derived cells (hUC) and provided by Guangzhou Future Homo Sapiens Institute of Biomedicine and Health (GFBH) [25]; UH1-F M1 derived from human cord blood CD34+ cells and human ES cell lines (H1, H9 and HN4) were obtained from the Chinese Academy of Sciences; HEK-293T, and human umbilical vein endothelial cell (HUVEC) cell lines were from ATCC. pMD2G (#12259) and psPAX2 (#12260) were from Addgene. .. The female NCG and nude mice (6–8 weeks) were purchased from Guangdong Medical Laboratory Animal Center and fed in a specific pathogen-free environment at the Experimental Animal Center of Jinan University. generate iPSC-ECs for the engineering of cellular heart valves.

    Article Title: Generation of individualized immunocompatible endothelial cells from HLA-I-matched human pluripotent stem cells.
    Article Snippet: .. Human iPSC lines: LHPb-YaabC55 (C55), LHPbYaabC56 (C56) and LHPa-MaaaC10 (C10) were derived from human urine-derived cells (hUC) and provided by Guangzhou Future Homo Sapiens Institute of Biomedicine and Health (GFBH) [25]; UH1-F M1 derived from human cord blood CD34+ cells and human ES cell lines (H1, H9 and HN4) were obtained from the Chinese Academy of Sciences; HEK-293T, and human umbilical vein endothelial cell (HUVEC) cell lines were from ATCC. pMD2G (#12259) and psPAX2 (#12260) were from Addgene. ..

    Article Title: Generation of individualized immunocompatible endothelial cells from HLA-I-matched human pluripotent stem cells
    Article Snippet: .. Human iPSC lines: LHPb-YaabC55 (C55), LHPb-YaabC56 (C56) and LHPa-MaaaC10 (C10) were derived from human urine-derived cells (hUC) and provided by Guangzhou Future Homo Sapiens Institute of Biomedicine and Health (GFBH) [ ]; UH1-F M1 derived from human cord blood CD34+ cells and human ES cell lines (H1, H9 and HN4) were obtained from the Chinese Academy of Sciences; HEK-293T, and human umbilical vein endothelial cell (HUVEC) cell lines were from ATCC. pMD2G (#12259) and psPAX2 (#12260) were from Addgene. .. The female NCG and nude mice (6–8 weeks) were purchased from Guangdong Medical Laboratory Animal Center and fed in a specific pathogen-free environment at the Experimental Animal Center of Jinan University.

    other:

    Article Title: A live single-cell reporter assay links intratumor heterogeneity to metastatic proclivity in Ewing sarcoma
    Article Snippet: HeLa cells [American Type Culture Collection (ATCC)] and A673 cells (ATCC) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco) whereas RD-ES cells (ATCC) were grown in RPMI (Gibco) supplemented with fetal bovine serum (FBS) (10%, PAN-Biotech) and penicillin-streptomycin (1%; Gibco).

    Marker:

    Article Title: The dynamics of the electrotactic reaction of mouse 3T3 fibroblasts.
    Article Snippet: .. Lentiviral vectors silencing the mouse Kcnj15 gene and coexpressing the GFP marker gene were produced in the HEK293T/17 (ATCC) packaging cell line by triple transfection with pLenti-Kcnj15-siRNA-GFP lentivector (ABM, Cat. No. i033064) and the packing plasmids psPAX2 and pMD2G (Addgene, #12260 and #12259, respectively) using S. Lasota et al. BBA - Molecular Cell Research 1871 (2024) 119647 Lipofectamine 2000 according to the protocol provided by the producer. .. Control vectors, coding nontargeting shRNA or GFP only, were prepared with pLKO1-shRNA-nontargeting plasmid (Sigma–Aldrich) and the pEGIP plasmid (Addgene #26777) in the same way.

    Produced:

    Article Title: The dynamics of the electrotactic reaction of mouse 3T3 fibroblasts.
    Article Snippet: .. Lentiviral vectors silencing the mouse Kcnj15 gene and coexpressing the GFP marker gene were produced in the HEK293T/17 (ATCC) packaging cell line by triple transfection with pLenti-Kcnj15-siRNA-GFP lentivector (ABM, Cat. No. i033064) and the packing plasmids psPAX2 and pMD2G (Addgene, #12260 and #12259, respectively) using S. Lasota et al. BBA - Molecular Cell Research 1871 (2024) 119647 Lipofectamine 2000 according to the protocol provided by the producer. .. Control vectors, coding nontargeting shRNA or GFP only, were prepared with pLKO1-shRNA-nontargeting plasmid (Sigma–Aldrich) and the pEGIP plasmid (Addgene #26777) in the same way.

    Transfection:

    Article Title: The dynamics of the electrotactic reaction of mouse 3T3 fibroblasts.
    Article Snippet: .. Lentiviral vectors silencing the mouse Kcnj15 gene and coexpressing the GFP marker gene were produced in the HEK293T/17 (ATCC) packaging cell line by triple transfection with pLenti-Kcnj15-siRNA-GFP lentivector (ABM, Cat. No. i033064) and the packing plasmids psPAX2 and pMD2G (Addgene, #12260 and #12259, respectively) using S. Lasota et al. BBA - Molecular Cell Research 1871 (2024) 119647 Lipofectamine 2000 according to the protocol provided by the producer. .. Control vectors, coding nontargeting shRNA or GFP only, were prepared with pLKO1-shRNA-nontargeting plasmid (Sigma–Aldrich) and the pEGIP plasmid (Addgene #26777) in the same way.

    Article Title: Tumor-targeted IL2 promotes specific CD8 + T cells private clonal expansion enhancing lymphoma control
    Article Snippet: The lentiviral plasmids pSicoR- Ef1a-mCh (Addgene, 31,847) was purified using a Plasmid Maxi Kit (QIAGEN, 12162). .. We then transfected HEK293T cells (ATCC, CRL-3216) using pMD2G and psPAX (Addgene, 12260 and 12259) as packaging vectors to generate viral particles. ..

    Article Title: A live single-cell reporter assay links intratumor heterogeneity to metastatic proclivity in Ewing sarcoma.
    Article Snippet: Spheroids of A673 and RD-ES cells were grown in ultralow attachment plates (Corning) and used in clonogenic and Matrigel-invasion assays. .. Lentiviral infection and in vitro induction For lentiviral production, HEK 293T cells (ATCC) were transfected using FuGENE 6 Transfection Reagent (Promega). pMD2G (Addgene, #12259) and pCMV∆R8.74 (Addgene, #12263) vectors were used as envelope and packaging plasmids, respectively. .. Lentiviral harvest was performed using Lenti-X Concentrator (TAKARA).

    Infection:

    Article Title: A live single-cell reporter assay links intratumor heterogeneity to metastatic proclivity in Ewing sarcoma.
    Article Snippet: Spheroids of A673 and RD-ES cells were grown in ultralow attachment plates (Corning) and used in clonogenic and Matrigel-invasion assays. .. Lentiviral infection and in vitro induction For lentiviral production, HEK 293T cells (ATCC) were transfected using FuGENE 6 Transfection Reagent (Promega). pMD2G (Addgene, #12259) and pCMV∆R8.74 (Addgene, #12263) vectors were used as envelope and packaging plasmids, respectively. .. Lentiviral harvest was performed using Lenti-X Concentrator (TAKARA).

    In Vitro:

    Article Title: A live single-cell reporter assay links intratumor heterogeneity to metastatic proclivity in Ewing sarcoma.
    Article Snippet: Spheroids of A673 and RD-ES cells were grown in ultralow attachment plates (Corning) and used in clonogenic and Matrigel-invasion assays. .. Lentiviral infection and in vitro induction For lentiviral production, HEK 293T cells (ATCC) were transfected using FuGENE 6 Transfection Reagent (Promega). pMD2G (Addgene, #12259) and pCMV∆R8.74 (Addgene, #12263) vectors were used as envelope and packaging plasmids, respectively. .. Lentiviral harvest was performed using Lenti-X Concentrator (TAKARA).



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