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plcb1 overexpression plasmids  (Obio Technology Corp Ltd)
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Obio Technology Corp Ltd plcb1 overexpression plasmids
Schematic diagram. Preparation and functional mechanisms of sEVs miR−423−5p in promoting periodontal regeneration. miR-423–5p was incubated with Cell-Penetrating Peptide (CPP) for 15 min at room temperature to form a complex. The positively charged surface of CPP promoted their adsorption onto negatively charged sEVs, facilitating entry through electrostatic and hydrophobic interactions. Following CPP degradation, engineered sEVs enriched with miR-423–5p (known as sEVs miR−423−5p ) were successfully generated. Once internalized by PDLCs via endocytosis, membrane fusion, or ligand–receptor interactions, sEVs miR−423−5p promoted PDLC proliferation. The released miR-423–5p silenced <t>PLCB1,</t> thereby enhancing osteogenic differentiation and facilitating the formation of the periodontal complex—including cementum, periodontal ligament, and alveolar bone—by expanding Sfrp2 + fibroblasts in the defect region.
Plcb1 Overexpression Plasmids, supplied by Obio Technology Corp Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plcb1/overexpression+plasmids+plcb1/pmc12703869-214-10-13
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plcb1 overexpression plasmids - by Bioz Stars, 2026-10
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a1971 abclonal china pi3k3  (ABclonal Biotechnology)
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ABclonal Biotechnology a1971 abclonal china pi3k3
Schematic diagram. Preparation and functional mechanisms of sEVs miR−423−5p in promoting periodontal regeneration. miR-423–5p was incubated with Cell-Penetrating Peptide (CPP) for 15 min at room temperature to form a complex. The positively charged surface of CPP promoted their adsorption onto negatively charged sEVs, facilitating entry through electrostatic and hydrophobic interactions. Following CPP degradation, engineered sEVs enriched with miR-423–5p (known as sEVs miR−423−5p ) were successfully generated. Once internalized by PDLCs via endocytosis, membrane fusion, or ligand–receptor interactions, sEVs miR−423−5p promoted PDLC proliferation. The released miR-423–5p silenced <t>PLCB1,</t> thereby enhancing osteogenic differentiation and facilitating the formation of the periodontal complex—including cementum, periodontal ligament, and alveolar bone—by expanding Sfrp2 + fibroblasts in the defect region.
A1971 Abclonal China Pi3k3, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plcb1/PLCB1+Rabbit+pAb/pm41351824-645-14-15
Average 93 stars, based on 1 article reviews
a1971 abclonal china pi3k3 - by Bioz Stars, 2026-10
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gene exp plcb1 hs01001930 m1  (Thermo Fisher)
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Thermo Fisher gene exp plcb1 hs01001930 m1
Schematic diagram. Preparation and functional mechanisms of sEVs miR−423−5p in promoting periodontal regeneration. miR-423–5p was incubated with Cell-Penetrating Peptide (CPP) for 15 min at room temperature to form a complex. The positively charged surface of CPP promoted their adsorption onto negatively charged sEVs, facilitating entry through electrostatic and hydrophobic interactions. Following CPP degradation, engineered sEVs enriched with miR-423–5p (known as sEVs miR−423−5p ) were successfully generated. Once internalized by PDLCs via endocytosis, membrane fusion, or ligand–receptor interactions, sEVs miR−423−5p promoted PDLC proliferation. The released miR-423–5p silenced <t>PLCB1,</t> thereby enhancing osteogenic differentiation and facilitating the formation of the periodontal complex—including cementum, periodontal ligament, and alveolar bone—by expanding Sfrp2 + fibroblasts in the defect region.
Gene Exp Plcb1 Hs01001930 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plcb1/Gene+Exp%2E+PLCB1%2C+Hs01001930_m1/pm41083694-206-19-24
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gene exp plcb1 hs01001930 m1 - by Bioz Stars, 2026-10
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plcb1  (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology plcb1
Schematic diagram. Preparation and functional mechanisms of sEVs miR−423−5p in promoting periodontal regeneration. miR-423–5p was incubated with Cell-Penetrating Peptide (CPP) for 15 min at room temperature to form a complex. The positively charged surface of CPP promoted their adsorption onto negatively charged sEVs, facilitating entry through electrostatic and hydrophobic interactions. Following CPP degradation, engineered sEVs enriched with miR-423–5p (known as sEVs miR−423−5p ) were successfully generated. Once internalized by PDLCs via endocytosis, membrane fusion, or ligand–receptor interactions, sEVs miR−423−5p promoted PDLC proliferation. The released miR-423–5p silenced <t>PLCB1,</t> thereby enhancing osteogenic differentiation and facilitating the formation of the periodontal complex—including cementum, periodontal ligament, and alveolar bone—by expanding Sfrp2 + fibroblasts in the defect region.
Plcb1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plcb1/PLC+%CE%B21+Antibody/pm40865635-83-29-30
Average 93 stars, based on 1 article reviews
plcb1 - by Bioz Stars, 2026-10
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plcb1  (ABclonal Biotechnology)
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ABclonal Biotechnology plcb1
Schematic diagram. Preparation and functional mechanisms of sEVs miR−423−5p in promoting periodontal regeneration. miR-423–5p was incubated with Cell-Penetrating Peptide (CPP) for 15 min at room temperature to form a complex. The positively charged surface of CPP promoted their adsorption onto negatively charged sEVs, facilitating entry through electrostatic and hydrophobic interactions. Following CPP degradation, engineered sEVs enriched with miR-423–5p (known as sEVs miR−423−5p ) were successfully generated. Once internalized by PDLCs via endocytosis, membrane fusion, or ligand–receptor interactions, sEVs miR−423−5p promoted PDLC proliferation. The released miR-423–5p silenced <t>PLCB1,</t> thereby enhancing osteogenic differentiation and facilitating the formation of the periodontal complex—including cementum, periodontal ligament, and alveolar bone—by expanding Sfrp2 + fibroblasts in the defect region.
Plcb1, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plcb1/PLCB1+Rabbit+pAb/pm39810414__es4c09276_si_001-45-16-21
Average 93 stars, based on 1 article reviews
plcb1 - by Bioz Stars, 2026-10
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rabbit anti smad6  (ABclonal Biotechnology)
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ABclonal Biotechnology rabbit anti smad6
Schematic diagram. Preparation and functional mechanisms of sEVs miR−423−5p in promoting periodontal regeneration. miR-423–5p was incubated with Cell-Penetrating Peptide (CPP) for 15 min at room temperature to form a complex. The positively charged surface of CPP promoted their adsorption onto negatively charged sEVs, facilitating entry through electrostatic and hydrophobic interactions. Following CPP degradation, engineered sEVs enriched with miR-423–5p (known as sEVs miR−423−5p ) were successfully generated. Once internalized by PDLCs via endocytosis, membrane fusion, or ligand–receptor interactions, sEVs miR−423−5p promoted PDLC proliferation. The released miR-423–5p silenced <t>PLCB1,</t> thereby enhancing osteogenic differentiation and facilitating the formation of the periodontal complex—including cementum, periodontal ligament, and alveolar bone—by expanding Sfrp2 + fibroblasts in the defect region.
Rabbit Anti Smad6, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plcb1/PLCB1+Rabbit+pAb/pm39433087-100-30-32
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rabbit anti smad6 - by Bioz Stars, 2026-10
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plcb1  (Sangon Biotech)
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Sangon Biotech plcb1
Schematic diagram. Preparation and functional mechanisms of sEVs miR−423−5p in promoting periodontal regeneration. miR-423–5p was incubated with Cell-Penetrating Peptide (CPP) for 15 min at room temperature to form a complex. The positively charged surface of CPP promoted their adsorption onto negatively charged sEVs, facilitating entry through electrostatic and hydrophobic interactions. Following CPP degradation, engineered sEVs enriched with miR-423–5p (known as sEVs miR−423−5p ) were successfully generated. Once internalized by PDLCs via endocytosis, membrane fusion, or ligand–receptor interactions, sEVs miR−423−5p promoted PDLC proliferation. The released miR-423–5p silenced <t>PLCB1,</t> thereby enhancing osteogenic differentiation and facilitating the formation of the periodontal complex—including cementum, periodontal ligament, and alveolar bone—by expanding Sfrp2 + fibroblasts in the defect region.
Plcb1, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plcb1/plcb1/pm38778543-31-8-12
Average 86 stars, based on 1 article reviews
plcb1 - by Bioz Stars, 2026-10
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anti plcb1  (Proteintech)
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Proteintech anti plcb1
Schematic diagram. Preparation and functional mechanisms of sEVs miR−423−5p in promoting periodontal regeneration. miR-423–5p was incubated with Cell-Penetrating Peptide (CPP) for 15 min at room temperature to form a complex. The positively charged surface of CPP promoted their adsorption onto negatively charged sEVs, facilitating entry through electrostatic and hydrophobic interactions. Following CPP degradation, engineered sEVs enriched with miR-423–5p (known as sEVs miR−423−5p ) were successfully generated. Once internalized by PDLCs via endocytosis, membrane fusion, or ligand–receptor interactions, sEVs miR−423−5p promoted PDLC proliferation. The released miR-423–5p silenced <t>PLCB1,</t> thereby enhancing osteogenic differentiation and facilitating the formation of the periodontal complex—including cementum, periodontal ligament, and alveolar bone—by expanding Sfrp2 + fibroblasts in the defect region.
Anti Plcb1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plcb1/Phospholipase+C+beta+1+Antibody/pm38778543-39-16-18
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anti plcb1 - by Bioz Stars, 2026-10
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plasmids pcmv6 xl6 plcb1a  (OriGene)
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OriGene plasmids pcmv6 xl6 plcb1a
Schematic diagram. Preparation and functional mechanisms of sEVs miR−423−5p in promoting periodontal regeneration. miR-423–5p was incubated with Cell-Penetrating Peptide (CPP) for 15 min at room temperature to form a complex. The positively charged surface of CPP promoted their adsorption onto negatively charged sEVs, facilitating entry through electrostatic and hydrophobic interactions. Following CPP degradation, engineered sEVs enriched with miR-423–5p (known as sEVs miR−423−5p ) were successfully generated. Once internalized by PDLCs via endocytosis, membrane fusion, or ligand–receptor interactions, sEVs miR−423−5p promoted PDLC proliferation. The released miR-423–5p silenced <t>PLCB1,</t> thereby enhancing osteogenic differentiation and facilitating the formation of the periodontal complex—including cementum, periodontal ligament, and alveolar bone—by expanding Sfrp2 + fibroblasts in the defect region.
Plasmids Pcmv6 Xl6 Plcb1a, supplied by OriGene, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Schematic diagram. Preparation and functional mechanisms of sEVs miR−423−5p in promoting periodontal regeneration. miR-423–5p was incubated with Cell-Penetrating Peptide (CPP) for 15 min at room temperature to form a complex. The positively charged surface of CPP promoted their adsorption onto negatively charged sEVs, facilitating entry through electrostatic and hydrophobic interactions. Following CPP degradation, engineered sEVs enriched with miR-423–5p (known as sEVs miR−423−5p ) were successfully generated. Once internalized by PDLCs via endocytosis, membrane fusion, or ligand–receptor interactions, sEVs miR−423−5p promoted PDLC proliferation. The released miR-423–5p silenced PLCB1, thereby enhancing osteogenic differentiation and facilitating the formation of the periodontal complex—including cementum, periodontal ligament, and alveolar bone—by expanding Sfrp2 + fibroblasts in the defect region.

Journal: Bioactive Materials

Article Title: miR-423-5p-enriched small extracellular vesicles drive periodontal regeneration via Sfrp2+ cell expansion

doi: 10.1016/j.bioactmat.2025.11.026

Figure Lengend Snippet: Schematic diagram. Preparation and functional mechanisms of sEVs miR−423−5p in promoting periodontal regeneration. miR-423–5p was incubated with Cell-Penetrating Peptide (CPP) for 15 min at room temperature to form a complex. The positively charged surface of CPP promoted their adsorption onto negatively charged sEVs, facilitating entry through electrostatic and hydrophobic interactions. Following CPP degradation, engineered sEVs enriched with miR-423–5p (known as sEVs miR−423−5p ) were successfully generated. Once internalized by PDLCs via endocytosis, membrane fusion, or ligand–receptor interactions, sEVs miR−423−5p promoted PDLC proliferation. The released miR-423–5p silenced PLCB1, thereby enhancing osteogenic differentiation and facilitating the formation of the periodontal complex—including cementum, periodontal ligament, and alveolar bone—by expanding Sfrp2 + fibroblasts in the defect region.

Article Snippet: PDLCs were transfected with siPLCB1 (TSINGKE BIOTECH, Beijing, China) and PLCB1 overexpression plasmids (OBIO Technology, Shanghai, China) according to the LipofectamineTM 3000 protocol (Invitrogen, Carlsbad, CA, USA).

Techniques: Functional Assay, Incubation, Adsorption, Generated, Membrane

miR-423–5p was identified as a key osteogenic factor in sEVs. (A) Schematic illustration of the experimental design used to assess the impact of miR-423–5p on PDLC proliferation and osteogenic differentiation. n = 3. ( B ) EdU assay evaluating the influence of miR-423–5p on PDLC proliferation. Green fluorescence indicates EdU-labeled cells, and blue fluorescence indicates DAPI-stained nuclei. Scale bar = 100 μm. ( C ) Quantitative assessment of EdU-positive cell proportions. n = 5. ( D ) CCK-8 experiment evaluating the impact of miR-423–5p on PDLC proliferation. n = 6. ( E ) ALP and ARS assays assessing the impact of miR-423–5p on PDLC osteogenic differentiation. Scale bar = 200 μm. ( F ) Quantitative analysis of ALP activity. n = 6. ( G ) Western blot analysis of the influence of miR-423–5p on the osteogenic proteins expression (COL1, Runx2) in PDLCs. ( H ) Quantitative analysis of protein expression. n = 3. ( I ) The influence of miR-423–5p on the expression of osteogenic genes COL1 and Runx2 in PDLCs, evaluated using a PCR assay. n = 3. ( J ) Venn diagram showing the target genes predicted by miRTarBase, miRWalk, and TargetScan. ( K ) Pathway interaction map of the intersecting target genes. ( L ) ALP and ARS assays assessing the impact of PLCB1 on osteogenic differentiation in PDLCs. Scale bar = 200 μm. ( M ) Quantitative analysis of ALP activity. n = 6. ( N ) ALP staining and quantitative analysis of ALP activity in PDLCs co-transfected with miR-423–5p mimic and PLCB1 overexpression plasmid (n = 6). ( O ) Effects of miR-423–5p mimic combined with PLCB1 overexpression on the expression levels of PLCB1, Wnt3, and β-catenin in PDLCs.

Journal: Bioactive Materials

Article Title: miR-423-5p-enriched small extracellular vesicles drive periodontal regeneration via Sfrp2+ cell expansion

doi: 10.1016/j.bioactmat.2025.11.026

Figure Lengend Snippet: miR-423–5p was identified as a key osteogenic factor in sEVs. (A) Schematic illustration of the experimental design used to assess the impact of miR-423–5p on PDLC proliferation and osteogenic differentiation. n = 3. ( B ) EdU assay evaluating the influence of miR-423–5p on PDLC proliferation. Green fluorescence indicates EdU-labeled cells, and blue fluorescence indicates DAPI-stained nuclei. Scale bar = 100 μm. ( C ) Quantitative assessment of EdU-positive cell proportions. n = 5. ( D ) CCK-8 experiment evaluating the impact of miR-423–5p on PDLC proliferation. n = 6. ( E ) ALP and ARS assays assessing the impact of miR-423–5p on PDLC osteogenic differentiation. Scale bar = 200 μm. ( F ) Quantitative analysis of ALP activity. n = 6. ( G ) Western blot analysis of the influence of miR-423–5p on the osteogenic proteins expression (COL1, Runx2) in PDLCs. ( H ) Quantitative analysis of protein expression. n = 3. ( I ) The influence of miR-423–5p on the expression of osteogenic genes COL1 and Runx2 in PDLCs, evaluated using a PCR assay. n = 3. ( J ) Venn diagram showing the target genes predicted by miRTarBase, miRWalk, and TargetScan. ( K ) Pathway interaction map of the intersecting target genes. ( L ) ALP and ARS assays assessing the impact of PLCB1 on osteogenic differentiation in PDLCs. Scale bar = 200 μm. ( M ) Quantitative analysis of ALP activity. n = 6. ( N ) ALP staining and quantitative analysis of ALP activity in PDLCs co-transfected with miR-423–5p mimic and PLCB1 overexpression plasmid (n = 6). ( O ) Effects of miR-423–5p mimic combined with PLCB1 overexpression on the expression levels of PLCB1, Wnt3, and β-catenin in PDLCs.

Article Snippet: PDLCs were transfected with siPLCB1 (TSINGKE BIOTECH, Beijing, China) and PLCB1 overexpression plasmids (OBIO Technology, Shanghai, China) according to the LipofectamineTM 3000 protocol (Invitrogen, Carlsbad, CA, USA).

Techniques: EdU Assay, Fluorescence, Labeling, Staining, CCK-8 Assay, Activity Assay, Western Blot, Expressing, Transfection, Over Expression, Plasmid Preparation