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Image Search Results
Journal: Frontiers in Molecular Neuroscience
Article Title: Expression of genes encoding the calcium signalosome in cellular and transgenic models of Huntington's disease
doi: 10.3389/fnmol.2013.00042
Figure Lengend Snippet: List of genes and their annotations included in the RT-qPCR array .
Article Snippet: Plcb1 , phospholipase C, beta 1 ,
Techniques: Binding Assay
Journal: International Journal of Molecular Sciences
Article Title: Elucidating the Role of circTIAM1 in Guangling Large-Tailed Sheep Adipocyte Proliferation and Differentiation via the miR-485-3p/PLCB1 Pathway
doi: 10.3390/ijms25094588
Figure Lengend Snippet: PLCB1; a target of miR-485-3p. ( A ) Predicted binding sites between miR-485-3p and PLCB1. ( B ) Binding and mutation sites of PLCB1 3′UTR with miR-485-3p. ( C ) Luciferase activity of wildtype and mutant PLCB1 3′UTR vectors cotransfected with miR-485-3p mimic or miR-485-3p NC into 293 T cells after 48 h. ( D – F ) Inhibition of PLCB1 expression by miR-485-3p at mRNA and protein levels in adipocytes. * p < 0.05, ** p < 0.01.
Article Snippet: Primary antibodies used for Western blotting were cyclin D3 (1:2000, 26755-1-AP, proteintech, Wuhan, China), CDK4 (1:2000, bs-0633R, Bioss, Beijing, China), PPARG (1:2000, 16643-1-AP, proteintech, Wuhan, China), C/EBPα (1:2000, 18311-1-AP, proteintech, Wuhan, China), FABP4 (1:1000, 12802-1-AP, proteintech, Wuhan, China), Adiponectin (1:1000, bs-0471R, Bioss, Beijing, China),
Techniques: Binding Assay, Mutagenesis, Luciferase, Activity Assay, Inhibition, Expressing
Journal: International Journal of Molecular Sciences
Article Title: Elucidating the Role of circTIAM1 in Guangling Large-Tailed Sheep Adipocyte Proliferation and Differentiation via the miR-485-3p/PLCB1 Pathway
doi: 10.3390/ijms25094588
Figure Lengend Snippet: circTIAM1 as a ceRNA for miR-485-3p. ( A ) Predicted binding sites between circTIAM1 and miR-485-3p. ( B ) Binding and mutation sites of circTIAM1 with miR-485-3p. ( C ) Luciferase activity of wildtype and mutant circTIAM1 vectors cotransfected with miR-485-3p mimic or miR-485-3p NC into 293 T cells after 48 h. ( D ) circTIAM1, miR-485-3p, and PLCB1 expression patterns during adipocyte differentiation. ( E ) qRTPCR analysis of mRNA of cyclin D3, CDK4, cyclin B, and PCNA expression levels. ( F ) qRTPCR analysis of mRNA of Adiponectin, C/EBPα, FABP4, and PPARγ expression levels. * p < 0.05, ** p < 0.01.
Article Snippet: Primary antibodies used for Western blotting were cyclin D3 (1:2000, 26755-1-AP, proteintech, Wuhan, China), CDK4 (1:2000, bs-0633R, Bioss, Beijing, China), PPARG (1:2000, 16643-1-AP, proteintech, Wuhan, China), C/EBPα (1:2000, 18311-1-AP, proteintech, Wuhan, China), FABP4 (1:1000, 12802-1-AP, proteintech, Wuhan, China), Adiponectin (1:1000, bs-0471R, Bioss, Beijing, China),
Techniques: Binding Assay, Mutagenesis, Luciferase, Activity Assay, Expressing
Journal: International Journal of Molecular Sciences
Article Title: Elucidating the Role of circTIAM1 in Guangling Large-Tailed Sheep Adipocyte Proliferation and Differentiation via the miR-485-3p/PLCB1 Pathway
doi: 10.3390/ijms25094588
Figure Lengend Snippet: Mechanism of circTIAM1 regulation in Guangling Large-Tailed sheep adipocyte proliferation and differentiation through the miR-485-3p/PLCB1 Pathway.
Article Snippet: Primary antibodies used for Western blotting were cyclin D3 (1:2000, 26755-1-AP, proteintech, Wuhan, China), CDK4 (1:2000, bs-0633R, Bioss, Beijing, China), PPARG (1:2000, 16643-1-AP, proteintech, Wuhan, China), C/EBPα (1:2000, 18311-1-AP, proteintech, Wuhan, China), FABP4 (1:1000, 12802-1-AP, proteintech, Wuhan, China), Adiponectin (1:1000, bs-0471R, Bioss, Beijing, China),
Techniques:
Journal: International Journal of Molecular Sciences
Article Title: Elucidating the Role of circTIAM1 in Guangling Large-Tailed Sheep Adipocyte Proliferation and Differentiation via the miR-485-3p/PLCB1 Pathway
doi: 10.3390/ijms25094588
Figure Lengend Snippet: shRNA sequences targeting PLCB1 † .
Article Snippet: Primary antibodies used for Western blotting were cyclin D3 (1:2000, 26755-1-AP, proteintech, Wuhan, China), CDK4 (1:2000, bs-0633R, Bioss, Beijing, China), PPARG (1:2000, 16643-1-AP, proteintech, Wuhan, China), C/EBPα (1:2000, 18311-1-AP, proteintech, Wuhan, China), FABP4 (1:1000, 12802-1-AP, proteintech, Wuhan, China), Adiponectin (1:1000, bs-0471R, Bioss, Beijing, China),
Techniques: shRNA
Journal: International Journal of Molecular Sciences
Article Title: Elucidating the Role of circTIAM1 in Guangling Large-Tailed Sheep Adipocyte Proliferation and Differentiation via the miR-485-3p/PLCB1 Pathway
doi: 10.3390/ijms25094588
Figure Lengend Snippet: qPCR primers for mRNA application.
Article Snippet: Primary antibodies used for Western blotting were cyclin D3 (1:2000, 26755-1-AP, proteintech, Wuhan, China), CDK4 (1:2000, bs-0633R, Bioss, Beijing, China), PPARG (1:2000, 16643-1-AP, proteintech, Wuhan, China), C/EBPα (1:2000, 18311-1-AP, proteintech, Wuhan, China), FABP4 (1:1000, 12802-1-AP, proteintech, Wuhan, China), Adiponectin (1:1000, bs-0471R, Bioss, Beijing, China),
Techniques:
Journal: PLoS ONE
Article Title: Charge Shielding of PIP 2 by Cations Regulates Enzyme Activity of Phospholipase C
doi: 10.1371/journal.pone.0144432
Figure Lengend Snippet: Activity of PLCγ1 (A, B) and PLCδ1 (C, D) was measured as PLCβ1 in . Symbols and lines are experimental data and fitting with the mathematical model, respectively. n = 4–8 for PLCγ1 and n = 2–8 for PLCδ1.
Article Snippet: Recombinant human PLCβ1 and
Techniques: Activity Assay