Journal: PLoS ONE

Article Title: Charge Shielding of PIP 2 by Cations Regulates Enzyme Activity of Phospholipase C

doi: 10.1371/journal.pone.0144432

Figure Lengend Snippet: Activity of PLCγ1 (A, B) and PLCδ1 (C, D) was measured as PLCβ1 in . Symbols and lines are experimental data and fitting with the mathematical model, respectively. n = 4–8 for PLCγ1 and n = 2–8 for PLCδ1.

Article Snippet: Recombinant human PLCβ1 and PLCδ1 proteins were purchased from OriGene (Rockville, MD) and human PLCγ1 was kindly provided by from Dr. John Sondek (University of North Carolina).

Techniques: Activity Assay

Journal: NPJ Schizophrenia

Article Title: Isoform specific differences in phospholipase C beta 1 expression in the prefrontal cortex in schizophrenia and suicide

doi: 10.1038/s41537-017-0020-x

Figure Lengend Snippet: PLCB1 a and b mRNA ( a ), and PLCB1a ( b ) and PLCB1b ( c ) protein, levels measured in CNS from Chrm1, 2, 4 and 5 knockout ( −/− ) mice compared to WT mice. Error bars show median (Mdn) and IQR. Western blot image ( d ) of 5 ug human or mouse cortical homogenate, run in duplicate, probed with anti-PLCB1 antibody

Article Snippet: Madhara Udewela at the Florey Institute, Australia, and co-workers measured post-mortem levels of two PLCB1 variants (a and b) in two areas of the prefrontal cortex (BA9 and BA46) in schizophrenia, many of whom were suicide completers.

Techniques: Knock-Out, Western Blot