Journal: bioRxiv
Article Title: Autophagy regulates tumor growth and metastasis
doi: 10.1101/2023.10.31.564991
Figure Lengend Snippet: (A) Immunoblotting in A431 cells with or without overexpression of p62 and/or Twist1. (B) Luciferase reporter assay of the E-cadherin promoter with an intact (E-cad (−108) WT-Luc) or mutated (E-cad (−108) Mut-Luc) Ebox site in cells as in A. (C) Wound healing assay of cell migration in cells as in A. (D) MTS assay of cells proliferation of cells as in A. (E) Tumor growth from cells as in A. (F) Number of lung tumor nodules per at 14 weeks following injection. (G) Immunoblotting in A375 cells with or without p62 overexpression. (H) Tumor growth from cells as in G. (I) Immunoblotting in A375 cells transfected with or without knockdown of Atg7 or p62. (J) Tumor growth from cells as in I. (K) Immunoblotting in HaCaT cells with or without p62 knockdown and treated with or without EGF/TGF-β)over a time course. (L) qRT-PCR analysis of Twist1 in HaCaT as in K treated with EGF/TGF-β for 48 h. (M) qRT-PCR analysis of p62. Data are shown from three independent experiments (mean±S.D.). n=3. *, P <0.05; compared with WT Con cells (B); #, P <0.05; compared with the WT group (B); *, P <0.05; compared with the Con, A431-p62, and A431-Twist1 groups (E); **, P <0.01; compared with the A431-Twist1 group (F).
Article Snippet: PGL2 E-cad (108) WT-Luc and PGL2 E-cad (108) Ebox Mut-Luc were kindly provided by Dr. Eric R. Fearon (Addgene plasmids 19291 and 19290)( ).
Techniques: Western Blot, Over Expression, Luciferase, Reporter Assay, Wound Healing Assay, Migration, MTS Assay, Injection, Transfection, Knockdown, Quantitative RT-PCR
