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Promega pgemt-easy plasmid
Pgemt Easy Plasmid, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgemt/pm40531937-65-7-9?v=Promega
Average 90 stars, based on 1 article reviews
pgemt-easy plasmid - by Bioz Stars, 2026-07
90/100 stars

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Cloning and expression of BparChR gene from B. paramycoides S48 ( A ) PCR amplification of BparChR gene; Lane 1, DNA ladder (NEB, B7025); Lane 2, BparChR amplicon approximately 0.6 kb ( B ) <t>Recombinant</t> chromate reductase expression by SDS-PAGE; Lane 1. Protein marker (11–245 kDa, Lane 2,3 Induced protein of E. coli BL 21 (DE3) with p ET-28a , Lane 4: Induced protein of E. coli BL 21 (DE3) with p ET28 <t>Bpar</t> ChR . ( C ) Phylogenetic relationship of B. paramycoides S48 chromate reductase with other chromate reductase available in NCBI database, Neighbor-joining tree showed maximum identity with chromate reductase of B. paramycoides sp OV166.
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Cloning and expression of BparChR gene from B. paramycoides S48 ( A ) PCR amplification of BparChR gene; Lane 1, DNA ladder (NEB, B7025); Lane 2, BparChR amplicon approximately 0.6 kb ( B ) <t>Recombinant</t> chromate reductase expression by SDS-PAGE; Lane 1. Protein marker (11–245 kDa, Lane 2,3 Induced protein of E. coli BL 21 (DE3) with p ET-28a , Lane 4: Induced protein of E. coli BL 21 (DE3) with p ET28 <t>Bpar</t> ChR . ( C ) Phylogenetic relationship of B. paramycoides S48 chromate reductase with other chromate reductase available in NCBI database, Neighbor-joining tree showed maximum identity with chromate reductase of B. paramycoides sp OV166.
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Cloning and expression of BparChR gene from B. paramycoides S48 ( A ) PCR amplification of BparChR gene; Lane 1, DNA ladder (NEB, B7025); Lane 2, BparChR amplicon approximately 0.6 kb ( B ) <t>Recombinant</t> chromate reductase expression by SDS-PAGE; Lane 1. Protein marker (11–245 kDa, Lane 2,3 Induced protein of E. coli BL 21 (DE3) with p ET-28a , Lane 4: Induced protein of E. coli BL 21 (DE3) with p ET28 <t>Bpar</t> ChR . ( C ) Phylogenetic relationship of B. paramycoides S48 chromate reductase with other chromate reductase available in NCBI database, Neighbor-joining tree showed maximum identity with chromate reductase of B. paramycoides sp OV166.
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Cloning and expression of BparChR gene from B. paramycoides S48 ( A ) PCR amplification of BparChR gene; Lane 1, DNA ladder (NEB, B7025); Lane 2, BparChR amplicon approximately 0.6 kb ( B ) Recombinant chromate reductase expression by SDS-PAGE; Lane 1. Protein marker (11–245 kDa, Lane 2,3 Induced protein of E. coli BL 21 (DE3) with p ET-28a , Lane 4: Induced protein of E. coli BL 21 (DE3) with p ET28 Bpar ChR . ( C ) Phylogenetic relationship of B. paramycoides S48 chromate reductase with other chromate reductase available in NCBI database, Neighbor-joining tree showed maximum identity with chromate reductase of B. paramycoides sp OV166.

Journal: Scientific Reports

Article Title: Cloning and expression of chromate reductase from Bacillus paramycoides S48 for chromium remediation

doi: 10.1038/s41598-025-03412-x

Figure Lengend Snippet: Cloning and expression of BparChR gene from B. paramycoides S48 ( A ) PCR amplification of BparChR gene; Lane 1, DNA ladder (NEB, B7025); Lane 2, BparChR amplicon approximately 0.6 kb ( B ) Recombinant chromate reductase expression by SDS-PAGE; Lane 1. Protein marker (11–245 kDa, Lane 2,3 Induced protein of E. coli BL 21 (DE3) with p ET-28a , Lane 4: Induced protein of E. coli BL 21 (DE3) with p ET28 Bpar ChR . ( C ) Phylogenetic relationship of B. paramycoides S48 chromate reductase with other chromate reductase available in NCBI database, Neighbor-joining tree showed maximum identity with chromate reductase of B. paramycoides sp OV166.

Article Snippet: The recombinant pGEMT- Bpar ChR plasmid was sequenced by Macrogen, Netherland.

Techniques: Cloning, Expressing, Amplification, Recombinant, SDS Page, Marker