Journal: Scientific Reports
Article Title: Cloning and expression of chromate reductase from Bacillus paramycoides S48 for chromium remediation
doi: 10.1038/s41598-025-03412-x
Figure Lengend Snippet: Cloning and expression of BparChR gene from B. paramycoides S48 ( A ) PCR amplification of BparChR gene; Lane 1, DNA ladder (NEB, B7025); Lane 2, BparChR amplicon approximately 0.6 kb ( B ) Recombinant chromate reductase expression by SDS-PAGE; Lane 1. Protein marker (11–245 kDa, Lane 2,3 Induced protein of E. coli BL 21 (DE3) with p ET-28a , Lane 4: Induced protein of E. coli BL 21 (DE3) with p ET28 Bpar ChR . ( C ) Phylogenetic relationship of B. paramycoides S48 chromate reductase with other chromate reductase available in NCBI database, Neighbor-joining tree showed maximum identity with chromate reductase of B. paramycoides sp OV166.
Article Snippet: The recombinant pGEMT- Bpar ChR plasmid was sequenced by Macrogen, Netherland.
Techniques: Cloning, Expressing, Amplification, Recombinant, SDS Page, Marker