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vector pgbkt7 bd  (TaKaRa)


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    TaKaRa vector pgbkt7 bd
    Vector Pgbkt7 Bd, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 4592 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgbkt7/pGBKT7+DNA-BD+Vector/bio_rxiv__64898__2026__05__09__724007-261-17-25
    Average 99 stars, based on 4592 article reviews
    vector pgbkt7 bd - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Amplification:

    Article Title: SCEP3 initiates synapsis and implements crossover interference in Arabidopsis
    Article Snippet: .. The N terminus was obtained by digesting the full-length SCEP3 with BamHI, while the C terminus of SCEP3 was amplified with the primers C-Terminus-F and C-Terminus-R and cloned into pGADT7 and pGBKT7 ( www.takarabio.com/ ). ..

    Article Title: SCEP3 initiates synapsis and implements crossover interference in Arabidopsis.
    Article Snippet: .. The N terminus was obtained by digesting the full-length SCEP3 with BamHI, while the C terminus of SCEP3 was amplified with the primers C-Terminus-F and C-Terminus-R and cloned into pGADT7 and pGBKT7 (www.takarabio.com/). ..

    Article Title: Balancing rice blast resistance and growth through suppression of the E3 ubiquitin ligase OsRING80.
    Article Snippet: .. 20 Yeast-two-hybrid assays 21 The full-length coding sequences or specific domains of DEP2 and OsRING80 were 22 amplified and cloned into pGADT7 or pGBKT7 (Clontech) vectors. ..

    Article Title: Balancing rice blast resistance and growth through suppression of the E3 ubiquitin ligase OsRING80
    Article Snippet: .. The full-length coding sequences or specific domains of DEP2 and OsRING80 were amplified and cloned into pGADT7 or pGBKT7 (Clontech) vectors. ..

    Clone Assay:

    Article Title: SCEP3 initiates synapsis and implements crossover interference in Arabidopsis
    Article Snippet: .. The N terminus was obtained by digesting the full-length SCEP3 with BamHI, while the C terminus of SCEP3 was amplified with the primers C-Terminus-F and C-Terminus-R and cloned into pGADT7 and pGBKT7 ( www.takarabio.com/ ). ..

    Article Title: A SUMO interacting motif in the replication initiator protein of tomato yellow leaf curl virus is required for viral replication
    Article Snippet: .. For the GAL4 Y2H assay, all gene fragments were cloned into Gateway-compatible variants of pGADT7 and pGBKT7 (Clontech) ( ). .. Resulting plasmids were introduced into Saccharomyces cerevisiae strain PJ69-4α ( ) using a standard lithium acetate/single-stranded DNA/polyethylene glycol 3350 transformation protocol ( ).

    Article Title: SCEP3 initiates synapsis and implements crossover interference in Arabidopsis.
    Article Snippet: .. The N terminus was obtained by digesting the full-length SCEP3 with BamHI, while the C terminus of SCEP3 was amplified with the primers C-Terminus-F and C-Terminus-R and cloned into pGADT7 and pGBKT7 (www.takarabio.com/). ..

    Article Title: bHLH35 mediates specificity in plant responses to multiple stress conditions
    Article Snippet: .. The coding sequence (CDS) of bHLH35 was cloned into a modified pGBKT7 (Takara, CA, USA, 630443) vector harboring the GAL4 DNA-BD where the uracil (URA) marker was replaced by a histidine (HIS) selection marker using In-Fusion cloning kit (Takara, CA, USA, 638955). .. The resulting DNA-BD-bHLH35 plasmid was transformed into the yeast two-gold (Y2G) yeast strain (Takara, CA, USA, 630498) using the lithium acetate method ( ).

    Article Title: Balancing rice blast resistance and growth through suppression of the E3 ubiquitin ligase OsRING80.
    Article Snippet: .. 20 Yeast-two-hybrid assays 21 The full-length coding sequences or specific domains of DEP2 and OsRING80 were 22 amplified and cloned into pGADT7 or pGBKT7 (Clontech) vectors. ..

    Article Title: Balancing rice blast resistance and growth through suppression of the E3 ubiquitin ligase OsRING80
    Article Snippet: .. The full-length coding sequences or specific domains of DEP2 and OsRING80 were amplified and cloned into pGADT7 or pGBKT7 (Clontech) vectors. ..

    Y2H Assay:

    Article Title: A SUMO interacting motif in the replication initiator protein of tomato yellow leaf curl virus is required for viral replication
    Article Snippet: .. For the GAL4 Y2H assay, all gene fragments were cloned into Gateway-compatible variants of pGADT7 and pGBKT7 (Clontech) ( ). .. Resulting plasmids were introduced into Saccharomyces cerevisiae strain PJ69-4α ( ) using a standard lithium acetate/single-stranded DNA/polyethylene glycol 3350 transformation protocol ( ).

    Sequencing:

    Article Title: bHLH35 mediates specificity in plant responses to multiple stress conditions
    Article Snippet: .. The coding sequence (CDS) of bHLH35 was cloned into a modified pGBKT7 (Takara, CA, USA, 630443) vector harboring the GAL4 DNA-BD where the uracil (URA) marker was replaced by a histidine (HIS) selection marker using In-Fusion cloning kit (Takara, CA, USA, 638955). .. The resulting DNA-BD-bHLH35 plasmid was transformed into the yeast two-gold (Y2G) yeast strain (Takara, CA, USA, 630498) using the lithium acetate method ( ).

    Modification:

    Article Title: bHLH35 mediates specificity in plant responses to multiple stress conditions
    Article Snippet: .. The coding sequence (CDS) of bHLH35 was cloned into a modified pGBKT7 (Takara, CA, USA, 630443) vector harboring the GAL4 DNA-BD where the uracil (URA) marker was replaced by a histidine (HIS) selection marker using In-Fusion cloning kit (Takara, CA, USA, 638955). .. The resulting DNA-BD-bHLH35 plasmid was transformed into the yeast two-gold (Y2G) yeast strain (Takara, CA, USA, 630498) using the lithium acetate method ( ).

    Marker:

    Article Title: bHLH35 mediates specificity in plant responses to multiple stress conditions
    Article Snippet: .. The coding sequence (CDS) of bHLH35 was cloned into a modified pGBKT7 (Takara, CA, USA, 630443) vector harboring the GAL4 DNA-BD where the uracil (URA) marker was replaced by a histidine (HIS) selection marker using In-Fusion cloning kit (Takara, CA, USA, 638955). .. The resulting DNA-BD-bHLH35 plasmid was transformed into the yeast two-gold (Y2G) yeast strain (Takara, CA, USA, 630498) using the lithium acetate method ( ).

    Selection:

    Article Title: bHLH35 mediates specificity in plant responses to multiple stress conditions
    Article Snippet: .. The coding sequence (CDS) of bHLH35 was cloned into a modified pGBKT7 (Takara, CA, USA, 630443) vector harboring the GAL4 DNA-BD where the uracil (URA) marker was replaced by a histidine (HIS) selection marker using In-Fusion cloning kit (Takara, CA, USA, 638955). .. The resulting DNA-BD-bHLH35 plasmid was transformed into the yeast two-gold (Y2G) yeast strain (Takara, CA, USA, 630498) using the lithium acetate method ( ).

    Cloning:

    Article Title: bHLH35 mediates specificity in plant responses to multiple stress conditions
    Article Snippet: .. The coding sequence (CDS) of bHLH35 was cloned into a modified pGBKT7 (Takara, CA, USA, 630443) vector harboring the GAL4 DNA-BD where the uracil (URA) marker was replaced by a histidine (HIS) selection marker using In-Fusion cloning kit (Takara, CA, USA, 638955). .. The resulting DNA-BD-bHLH35 plasmid was transformed into the yeast two-gold (Y2G) yeast strain (Takara, CA, USA, 630498) using the lithium acetate method ( ).



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    Image Search Results


    Yeast 2‐hybrid interaction assay of the interaction between (a) TrAP from ToLCNDV‐C ([C]), ToLCNDV‐T&C ([T&C]), ToLCKV‐T ([T]) and ToLCNDV‐C[M9] ([C‐M9]) and SlAGO1, CsAGO1, Slrgs‐CaM and SlATG7. (b) REn from ToLCNDV‐C, ToLCNDV‐T&C, ToLCKV‐T and ToLCNDV‐C[M9] and SlPolA, SlRBR, CsPCNA and SlPCNA. TrAP was expressed as a GAL4‐activating domain fusion protein in HGold yeast and mated with Y187 yeast expressing the plant protein as a fusion protein to the GAL4‐DNA binding domain. The figure shows the mated yeast plated on triple dropout (−Trp/−Leu/−His) plates as selection for interaction. Empty vector controls are HGold expressing the empty vector pGADT7‐AD or Y187 expressing the empty pGBKT7‐BD vector.

    Journal: Molecular Plant Pathology

    Article Title: Viral and Host Factors Involved in Host Gain and Host Loss by Tomato Leaf Curl Begomoviruses in Tomato and Cucumbers

    doi: 10.1111/mpp.70202

    Figure Lengend Snippet: Yeast 2‐hybrid interaction assay of the interaction between (a) TrAP from ToLCNDV‐C ([C]), ToLCNDV‐T&C ([T&C]), ToLCKV‐T ([T]) and ToLCNDV‐C[M9] ([C‐M9]) and SlAGO1, CsAGO1, Slrgs‐CaM and SlATG7. (b) REn from ToLCNDV‐C, ToLCNDV‐T&C, ToLCKV‐T and ToLCNDV‐C[M9] and SlPolA, SlRBR, CsPCNA and SlPCNA. TrAP was expressed as a GAL4‐activating domain fusion protein in HGold yeast and mated with Y187 yeast expressing the plant protein as a fusion protein to the GAL4‐DNA binding domain. The figure shows the mated yeast plated on triple dropout (−Trp/−Leu/−His) plates as selection for interaction. Empty vector controls are HGold expressing the empty vector pGADT7‐AD or Y187 expressing the empty pGBKT7‐BD vector.

    Article Snippet: Full‐length ORFs of REn were obtained from ToLCNDV‐C, ToLCNDV‐C[M9], ToLCNDV‐T&C and ToLCKV‐T plasmids and cloned into pGBKT7‐BD, fusing with the Gal4 DNA‐binding domain (Takara: 630443).

    Techniques: Expressing, Binding Assay, Selection, Plasmid Preparation