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pgm3  (Proteintech)


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    Structured Review

    Proteintech pgm3
    Pgm3, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgam2/PGAM2+Antibody/pm41734653-96-10-12
    Average 93 stars, based on 10 article reviews
    pgm3 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Simple Western:

    Article Title: A ratiometric catalog of protein isoform shifts in the cardiac fetal gene program
    Article Snippet: Digital immunoblot analysis of the protein lysates was performed in the Jess Simple Western System (ProteinSimple) using the 12–230 kDa Separation module (Cat# SM-W004, ProteinSimple), according to the manufacturer’s instructions. .. The following primary antibodies and the dilution ratios were used in the Jess Simple Western: ENO1 (Cat# 11204-1-AP, Proteintech; 1:100), ENO3 (Cat# 55234-1- AP, Proteintech; 1:200), PGAM1 (Cat# NBP1-49532, Novus Biologicals; 1:40), PGAM2 (Cat# 15550-1- AP, Proteintech; 1:10), PPA-1 (Cat# 14985-1-AP, Proteintech; 1:10), PPA-2 (Cat# 16662-1-AP, Proteintech; 1:50), GAC-specific (Cat# 19958-1-AP, Proteintech; 1:50), and KGA-Specific antibody (Cat# 20170-1-AP, Proteintech; 1:50). .. Normalization was performed using a protein normalization reagent (Cat# DM-PN02, ProteinSimple) according to the manufacturer’s guidelines.

    Electrophoresis:

    Article Title: Circular RNA circMYLK4 shifts energy metabolism from glycolysis to OXPHOS by binding to the calcium channel auxiliary subunit CACNA2D2.
    Article Snippet: Received for publication, January 17, 2024, and in revised form, May 9, 2024 Published, Papers in Press, May 30, 2024, https://doi.org/10.1016/j.jbc.2024.107426 Haigang Cao, Chenchen Li, Xiaohui Sun, Jinjin Yang, Xiao Li, Gongshe Yang, Jianjun Jin*, and Xine Shi* From the Laboratory of Animal Fat Deposition and Muscle Development, Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China

    Incubation:

    Article Title: Circular RNA circMYLK4 shifts energy metabolism from glycolysis to OXPHOS by binding to the calcium channel auxiliary subunit CACNA2D2.
    Article Snippet: Received for publication, January 17, 2024, and in revised form, May 9, 2024 Published, Papers in Press, May 30, 2024, https://doi.org/10.1016/j.jbc.2024.107426 Haigang Cao, Chenchen Li, Xiaohui Sun, Jinjin Yang, Xiao Li, Gongshe Yang, Jianjun Jin*, and Xine Shi* From the Laboratory of Animal Fat Deposition and Muscle Development, Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China



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    A OCR, an index of oxygen consumption in mitochondria indicating oxidative phosphorylation coupled with glycolytic activity, was measured in 53KOLS cells expressing scramble or Rb shRNA. Cells were sequentially treated with 10.5 mM glucose and 100 mM 2-deoxy glucose. B ECAR, an index of the rate of lactate production indicating glycolytic activity, was measured in 53KOLS cells expressing scramble or Rb shRNA. Cells were sequentially treated with 10.5 mM glucose and 100 mM 2-deoxy glucose (2-DG). C Relative 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG) fluorescence intensity showing glucose uptake in 53KOLS cells expressing scramble or Rb shRNA. Paired student’s t-test was performed. * P < 0.05 and n.s., not significant. D ECAR was measured in 53KOLS cells expressing scramble or Rb shRNA following the treatment with 1 mM pyruvate. E ECAR was measured in 53KOLS. cells expressing scramble or Rb shRNA following the treatment with 4 mM glutamine. F OCR in 53KOLS cells expressing scramble or Rb shRNA was measured in the presence or absence of a glutaminase inhibitor, BPTES (5 μM). Tukey HSD test was performed. ** P < 0.01 and n.s., not significant. G Relative incorporation of [U- 14 C]-glucose into lipid. 53KOLS cells were cultured in the presence of [U- 14 C]-glucose (6.25 μCi/ml) for 24 h. 14 C activity was measured using liquid scintillation counter. Paired student’s t-test was performed. *** P < 0.001. H Relative incorporation of [U- 14 C]-glutamine into lipid. 53KOLS cells were cultured in the presence of [U- 14 C]-glutamine (62.5 μCi/ml) for 24 h. Paired student’s t-test was performed. n.s., not significant. I Relative lipid incorporation of 3 H 2 O. 53KOLS cells were cultured in the presence of 3 H 2 O (20 v/v%) for 24 h. 3 H activity was measured using liquid scintillation counter. Paired student’s t-test was performed. * P < 0.05. J IB of the indicated proteins in 53KOLS cells overexpressing <t>Pgam2.</t> K Relative lipid incorporation of [U- 14 C]-glucose in 53KOLS cells overexpressing Pgam2. Tukey’s HSD test was performed. * P < 0.05 and n.s., not significant. L ECAR was measured in AGS cells expressing scramble or RB1 shRNA. Cells were sequentially treated with 10.5 mM glucose and 100 mM 2-DG. M Relative 2-NBDG fluorescence intensity in AGS cells expressing scramble or RB1 shRNA. Tukey HSD test was performed. *** P < 0.001 and ** P < 0.01. N Relative 2-NBDG fluorescence intensity in SNU-638 cells expressing scramble or RB1 shRNA. Tukey HSD test was performed. ** P < 0.01 and ** P < 0.05. O Absolute glucose consumption rate within 24 h in SNU-638 cells expressing scramble or RB1 shRNA. Paired student’s t-test was performed. * P < 0.05. P Absolute lactate secretion rate within 24 h in SNU-638 cells expressing scramble or RB1 shRNA. Paired student’s t-test was performed. * P < 0.05. Q Relative incorporation of [U- 14 C]-glucose into lipid in AGS cells expressing scramble or RB1 shRNA those were cultured in the presence of [U- 14 C]-glucose (6.25 μCi/ml) for 24 h. 14 C activity was measured using liquid scintillation counter. Tukey HSD test was performed. ** P < 0.01 and * P < 0.05. R Effect of PGAM1 overexpression on glycolytic flow in RB1-depleted AGS cells. S Effect of PGAM1 overexpression on glucose consumption for 24 h in RB1-depleted AGS cells. Glucose concentration in medium was determined using colorimetric assay. Tukey HSD test was performed. * P < 0.05. All data are shown as the mean ± SEM.
    Gene Exp Pgam2 Mm01187768 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgam2/Gene+Exp%2E+Pgam2%2C+Mm01187768_m1/pmc12290115-392-20--1
    Average 94 stars, based on 1 article reviews
    gene exp pgam2 mm01187768 m1 - by Bioz Stars, 2026-09
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    A OCR, an index of oxygen consumption in mitochondria indicating oxidative phosphorylation coupled with glycolytic activity, was measured in 53KOLS cells expressing scramble or Rb shRNA. Cells were sequentially treated with 10.5 mM glucose and 100 mM 2-deoxy glucose. B ECAR, an index of the rate of lactate production indicating glycolytic activity, was measured in 53KOLS cells expressing scramble or Rb shRNA. Cells were sequentially treated with 10.5 mM glucose and 100 mM 2-deoxy glucose (2-DG). C Relative 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG) fluorescence intensity showing glucose uptake in 53KOLS cells expressing scramble or Rb shRNA. Paired student’s t-test was performed. * P < 0.05 and n.s., not significant. D ECAR was measured in 53KOLS cells expressing scramble or Rb shRNA following the treatment with 1 mM pyruvate. E ECAR was measured in 53KOLS. cells expressing scramble or Rb shRNA following the treatment with 4 mM glutamine. F OCR in 53KOLS cells expressing scramble or Rb shRNA was measured in the presence or absence of a glutaminase inhibitor, BPTES (5 μM). Tukey HSD test was performed. ** P < 0.01 and n.s., not significant. G Relative incorporation of [U- 14 C]-glucose into lipid. 53KOLS cells were cultured in the presence of [U- 14 C]-glucose (6.25 μCi/ml) for 24 h. 14 C activity was measured using liquid scintillation counter. Paired student’s t-test was performed. *** P < 0.001. H Relative incorporation of [U- 14 C]-glutamine into lipid. 53KOLS cells were cultured in the presence of [U- 14 C]-glutamine (62.5 μCi/ml) for 24 h. Paired student’s t-test was performed. n.s., not significant. I Relative lipid incorporation of 3 H 2 O. 53KOLS cells were cultured in the presence of 3 H 2 O (20 v/v%) for 24 h. 3 H activity was measured using liquid scintillation counter. Paired student’s t-test was performed. * P < 0.05. J IB of the indicated proteins in 53KOLS cells overexpressing <t>Pgam2.</t> K Relative lipid incorporation of [U- 14 C]-glucose in 53KOLS cells overexpressing Pgam2. Tukey’s HSD test was performed. * P < 0.05 and n.s., not significant. L ECAR was measured in AGS cells expressing scramble or RB1 shRNA. Cells were sequentially treated with 10.5 mM glucose and 100 mM 2-DG. M Relative 2-NBDG fluorescence intensity in AGS cells expressing scramble or RB1 shRNA. Tukey HSD test was performed. *** P < 0.001 and ** P < 0.01. N Relative 2-NBDG fluorescence intensity in SNU-638 cells expressing scramble or RB1 shRNA. Tukey HSD test was performed. ** P < 0.01 and ** P < 0.05. O Absolute glucose consumption rate within 24 h in SNU-638 cells expressing scramble or RB1 shRNA. Paired student’s t-test was performed. * P < 0.05. P Absolute lactate secretion rate within 24 h in SNU-638 cells expressing scramble or RB1 shRNA. Paired student’s t-test was performed. * P < 0.05. Q Relative incorporation of [U- 14 C]-glucose into lipid in AGS cells expressing scramble or RB1 shRNA those were cultured in the presence of [U- 14 C]-glucose (6.25 μCi/ml) for 24 h. 14 C activity was measured using liquid scintillation counter. Tukey HSD test was performed. ** P < 0.01 and * P < 0.05. R Effect of PGAM1 overexpression on glycolytic flow in RB1-depleted AGS cells. S Effect of PGAM1 overexpression on glucose consumption for 24 h in RB1-depleted AGS cells. Glucose concentration in medium was determined using colorimetric assay. Tukey HSD test was performed. * P < 0.05. All data are shown as the mean ± SEM.
    Pgam2 Enz 578, supplied by ProSpec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    pgam2 enz-578 - by Bioz Stars, 2026-09
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    pgm3  (Proteintech)
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    A OCR, an index of oxygen consumption in mitochondria indicating oxidative phosphorylation coupled with glycolytic activity, was measured in 53KOLS cells expressing scramble or Rb shRNA. Cells were sequentially treated with 10.5 mM glucose and 100 mM 2-deoxy glucose. B ECAR, an index of the rate of lactate production indicating glycolytic activity, was measured in 53KOLS cells expressing scramble or Rb shRNA. Cells were sequentially treated with 10.5 mM glucose and 100 mM 2-deoxy glucose (2-DG). C Relative 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG) fluorescence intensity showing glucose uptake in 53KOLS cells expressing scramble or Rb shRNA. Paired student’s t-test was performed. * P < 0.05 and n.s., not significant. D ECAR was measured in 53KOLS cells expressing scramble or Rb shRNA following the treatment with 1 mM pyruvate. E ECAR was measured in 53KOLS. cells expressing scramble or Rb shRNA following the treatment with 4 mM glutamine. F OCR in 53KOLS cells expressing scramble or Rb shRNA was measured in the presence or absence of a glutaminase inhibitor, BPTES (5 μM). Tukey HSD test was performed. ** P < 0.01 and n.s., not significant. G Relative incorporation of [U- 14 C]-glucose into lipid. 53KOLS cells were cultured in the presence of [U- 14 C]-glucose (6.25 μCi/ml) for 24 h. 14 C activity was measured using liquid scintillation counter. Paired student’s t-test was performed. *** P < 0.001. H Relative incorporation of [U- 14 C]-glutamine into lipid. 53KOLS cells were cultured in the presence of [U- 14 C]-glutamine (62.5 μCi/ml) for 24 h. Paired student’s t-test was performed. n.s., not significant. I Relative lipid incorporation of 3 H 2 O. 53KOLS cells were cultured in the presence of 3 H 2 O (20 v/v%) for 24 h. 3 H activity was measured using liquid scintillation counter. Paired student’s t-test was performed. * P < 0.05. J IB of the indicated proteins in 53KOLS cells overexpressing <t>Pgam2.</t> K Relative lipid incorporation of [U- 14 C]-glucose in 53KOLS cells overexpressing Pgam2. Tukey’s HSD test was performed. * P < 0.05 and n.s., not significant. L ECAR was measured in AGS cells expressing scramble or RB1 shRNA. Cells were sequentially treated with 10.5 mM glucose and 100 mM 2-DG. M Relative 2-NBDG fluorescence intensity in AGS cells expressing scramble or RB1 shRNA. Tukey HSD test was performed. *** P < 0.001 and ** P < 0.01. N Relative 2-NBDG fluorescence intensity in SNU-638 cells expressing scramble or RB1 shRNA. Tukey HSD test was performed. ** P < 0.01 and ** P < 0.05. O Absolute glucose consumption rate within 24 h in SNU-638 cells expressing scramble or RB1 shRNA. Paired student’s t-test was performed. * P < 0.05. P Absolute lactate secretion rate within 24 h in SNU-638 cells expressing scramble or RB1 shRNA. Paired student’s t-test was performed. * P < 0.05. Q Relative incorporation of [U- 14 C]-glucose into lipid in AGS cells expressing scramble or RB1 shRNA those were cultured in the presence of [U- 14 C]-glucose (6.25 μCi/ml) for 24 h. 14 C activity was measured using liquid scintillation counter. Tukey HSD test was performed. ** P < 0.01 and * P < 0.05. R Effect of PGAM1 overexpression on glycolytic flow in RB1-depleted AGS cells. S Effect of PGAM1 overexpression on glucose consumption for 24 h in RB1-depleted AGS cells. Glucose concentration in medium was determined using colorimetric assay. Tukey HSD test was performed. * P < 0.05. All data are shown as the mean ± SEM.
    Pgm3, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgam2/PGAM2+Antibody/pm41734653-96-10-12
    Average 93 stars, based on 1 article reviews
    pgm3 - by Bioz Stars, 2026-09
    93/100 stars
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    pgam2  (Proteintech)
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    A OCR, an index of oxygen consumption in mitochondria indicating oxidative phosphorylation coupled with glycolytic activity, was measured in 53KOLS cells expressing scramble or Rb shRNA. Cells were sequentially treated with 10.5 mM glucose and 100 mM 2-deoxy glucose. B ECAR, an index of the rate of lactate production indicating glycolytic activity, was measured in 53KOLS cells expressing scramble or Rb shRNA. Cells were sequentially treated with 10.5 mM glucose and 100 mM 2-deoxy glucose (2-DG). C Relative 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG) fluorescence intensity showing glucose uptake in 53KOLS cells expressing scramble or Rb shRNA. Paired student’s t-test was performed. * P < 0.05 and n.s., not significant. D ECAR was measured in 53KOLS cells expressing scramble or Rb shRNA following the treatment with 1 mM pyruvate. E ECAR was measured in 53KOLS. cells expressing scramble or Rb shRNA following the treatment with 4 mM glutamine. F OCR in 53KOLS cells expressing scramble or Rb shRNA was measured in the presence or absence of a glutaminase inhibitor, BPTES (5 μM). Tukey HSD test was performed. ** P < 0.01 and n.s., not significant. G Relative incorporation of [U- 14 C]-glucose into lipid. 53KOLS cells were cultured in the presence of [U- 14 C]-glucose (6.25 μCi/ml) for 24 h. 14 C activity was measured using liquid scintillation counter. Paired student’s t-test was performed. *** P < 0.001. H Relative incorporation of [U- 14 C]-glutamine into lipid. 53KOLS cells were cultured in the presence of [U- 14 C]-glutamine (62.5 μCi/ml) for 24 h. Paired student’s t-test was performed. n.s., not significant. I Relative lipid incorporation of 3 H 2 O. 53KOLS cells were cultured in the presence of 3 H 2 O (20 v/v%) for 24 h. 3 H activity was measured using liquid scintillation counter. Paired student’s t-test was performed. * P < 0.05. J IB of the indicated proteins in 53KOLS cells overexpressing <t>Pgam2.</t> K Relative lipid incorporation of [U- 14 C]-glucose in 53KOLS cells overexpressing Pgam2. Tukey’s HSD test was performed. * P < 0.05 and n.s., not significant. L ECAR was measured in AGS cells expressing scramble or RB1 shRNA. Cells were sequentially treated with 10.5 mM glucose and 100 mM 2-DG. M Relative 2-NBDG fluorescence intensity in AGS cells expressing scramble or RB1 shRNA. Tukey HSD test was performed. *** P < 0.001 and ** P < 0.01. N Relative 2-NBDG fluorescence intensity in SNU-638 cells expressing scramble or RB1 shRNA. Tukey HSD test was performed. ** P < 0.01 and ** P < 0.05. O Absolute glucose consumption rate within 24 h in SNU-638 cells expressing scramble or RB1 shRNA. Paired student’s t-test was performed. * P < 0.05. P Absolute lactate secretion rate within 24 h in SNU-638 cells expressing scramble or RB1 shRNA. Paired student’s t-test was performed. * P < 0.05. Q Relative incorporation of [U- 14 C]-glucose into lipid in AGS cells expressing scramble or RB1 shRNA those were cultured in the presence of [U- 14 C]-glucose (6.25 μCi/ml) for 24 h. 14 C activity was measured using liquid scintillation counter. Tukey HSD test was performed. ** P < 0.01 and * P < 0.05. R Effect of PGAM1 overexpression on glycolytic flow in RB1-depleted AGS cells. S Effect of PGAM1 overexpression on glucose consumption for 24 h in RB1-depleted AGS cells. Glucose concentration in medium was determined using colorimetric assay. Tukey HSD test was performed. * P < 0.05. All data are shown as the mean ± SEM.
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    Average 93 stars, based on 1 article reviews
    pgam2 - by Bioz Stars, 2026-09
    93/100 stars
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    ma5 25671  (Novus Biologicals)
    93
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    A OCR, an index of oxygen consumption in mitochondria indicating oxidative phosphorylation coupled with glycolytic activity, was measured in 53KOLS cells expressing scramble or Rb shRNA. Cells were sequentially treated with 10.5 mM glucose and 100 mM 2-deoxy glucose. B ECAR, an index of the rate of lactate production indicating glycolytic activity, was measured in 53KOLS cells expressing scramble or Rb shRNA. Cells were sequentially treated with 10.5 mM glucose and 100 mM 2-deoxy glucose (2-DG). C Relative 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG) fluorescence intensity showing glucose uptake in 53KOLS cells expressing scramble or Rb shRNA. Paired student’s t-test was performed. * P < 0.05 and n.s., not significant. D ECAR was measured in 53KOLS cells expressing scramble or Rb shRNA following the treatment with 1 mM pyruvate. E ECAR was measured in 53KOLS. cells expressing scramble or Rb shRNA following the treatment with 4 mM glutamine. F OCR in 53KOLS cells expressing scramble or Rb shRNA was measured in the presence or absence of a glutaminase inhibitor, BPTES (5 μM). Tukey HSD test was performed. ** P < 0.01 and n.s., not significant. G Relative incorporation of [U- 14 C]-glucose into lipid. 53KOLS cells were cultured in the presence of [U- 14 C]-glucose (6.25 μCi/ml) for 24 h. 14 C activity was measured using liquid scintillation counter. Paired student’s t-test was performed. *** P < 0.001. H Relative incorporation of [U- 14 C]-glutamine into lipid. 53KOLS cells were cultured in the presence of [U- 14 C]-glutamine (62.5 μCi/ml) for 24 h. Paired student’s t-test was performed. n.s., not significant. I Relative lipid incorporation of 3 H 2 O. 53KOLS cells were cultured in the presence of 3 H 2 O (20 v/v%) for 24 h. 3 H activity was measured using liquid scintillation counter. Paired student’s t-test was performed. * P < 0.05. J IB of the indicated proteins in 53KOLS cells overexpressing <t>Pgam2.</t> K Relative lipid incorporation of [U- 14 C]-glucose in 53KOLS cells overexpressing Pgam2. Tukey’s HSD test was performed. * P < 0.05 and n.s., not significant. L ECAR was measured in AGS cells expressing scramble or RB1 shRNA. Cells were sequentially treated with 10.5 mM glucose and 100 mM 2-DG. M Relative 2-NBDG fluorescence intensity in AGS cells expressing scramble or RB1 shRNA. Tukey HSD test was performed. *** P < 0.001 and ** P < 0.01. N Relative 2-NBDG fluorescence intensity in SNU-638 cells expressing scramble or RB1 shRNA. Tukey HSD test was performed. ** P < 0.01 and ** P < 0.05. O Absolute glucose consumption rate within 24 h in SNU-638 cells expressing scramble or RB1 shRNA. Paired student’s t-test was performed. * P < 0.05. P Absolute lactate secretion rate within 24 h in SNU-638 cells expressing scramble or RB1 shRNA. Paired student’s t-test was performed. * P < 0.05. Q Relative incorporation of [U- 14 C]-glucose into lipid in AGS cells expressing scramble or RB1 shRNA those were cultured in the presence of [U- 14 C]-glucose (6.25 μCi/ml) for 24 h. 14 C activity was measured using liquid scintillation counter. Tukey HSD test was performed. ** P < 0.01 and * P < 0.05. R Effect of PGAM1 overexpression on glycolytic flow in RB1-depleted AGS cells. S Effect of PGAM1 overexpression on glucose consumption for 24 h in RB1-depleted AGS cells. Glucose concentration in medium was determined using colorimetric assay. Tukey HSD test was performed. * P < 0.05. All data are shown as the mean ± SEM.
    Ma5 25671, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 1 article reviews
    ma5 25671 - by Bioz Stars, 2026-09
    93/100 stars
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    pgam2 antibody  (Beijing Solarbio Science)
    90
    Beijing Solarbio Science pgam2 antibody
    Primary antibodies and secondary antibody utilized in Western blotting.
    Pgam2 Antibody, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgam2/pgam2+antibody/pmc11503688-2-1-7
    Average 90 stars, based on 1 article reviews
    pgam2 antibody - by Bioz Stars, 2026-09
    90/100 stars
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    A OCR, an index of oxygen consumption in mitochondria indicating oxidative phosphorylation coupled with glycolytic activity, was measured in 53KOLS cells expressing scramble or Rb shRNA. Cells were sequentially treated with 10.5 mM glucose and 100 mM 2-deoxy glucose. B ECAR, an index of the rate of lactate production indicating glycolytic activity, was measured in 53KOLS cells expressing scramble or Rb shRNA. Cells were sequentially treated with 10.5 mM glucose and 100 mM 2-deoxy glucose (2-DG). C Relative 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG) fluorescence intensity showing glucose uptake in 53KOLS cells expressing scramble or Rb shRNA. Paired student’s t-test was performed. * P < 0.05 and n.s., not significant. D ECAR was measured in 53KOLS cells expressing scramble or Rb shRNA following the treatment with 1 mM pyruvate. E ECAR was measured in 53KOLS. cells expressing scramble or Rb shRNA following the treatment with 4 mM glutamine. F OCR in 53KOLS cells expressing scramble or Rb shRNA was measured in the presence or absence of a glutaminase inhibitor, BPTES (5 μM). Tukey HSD test was performed. ** P < 0.01 and n.s., not significant. G Relative incorporation of [U- 14 C]-glucose into lipid. 53KOLS cells were cultured in the presence of [U- 14 C]-glucose (6.25 μCi/ml) for 24 h. 14 C activity was measured using liquid scintillation counter. Paired student’s t-test was performed. *** P < 0.001. H Relative incorporation of [U- 14 C]-glutamine into lipid. 53KOLS cells were cultured in the presence of [U- 14 C]-glutamine (62.5 μCi/ml) for 24 h. Paired student’s t-test was performed. n.s., not significant. I Relative lipid incorporation of 3 H 2 O. 53KOLS cells were cultured in the presence of 3 H 2 O (20 v/v%) for 24 h. 3 H activity was measured using liquid scintillation counter. Paired student’s t-test was performed. * P < 0.05. J IB of the indicated proteins in 53KOLS cells overexpressing Pgam2. K Relative lipid incorporation of [U- 14 C]-glucose in 53KOLS cells overexpressing Pgam2. Tukey’s HSD test was performed. * P < 0.05 and n.s., not significant. L ECAR was measured in AGS cells expressing scramble or RB1 shRNA. Cells were sequentially treated with 10.5 mM glucose and 100 mM 2-DG. M Relative 2-NBDG fluorescence intensity in AGS cells expressing scramble or RB1 shRNA. Tukey HSD test was performed. *** P < 0.001 and ** P < 0.01. N Relative 2-NBDG fluorescence intensity in SNU-638 cells expressing scramble or RB1 shRNA. Tukey HSD test was performed. ** P < 0.01 and ** P < 0.05. O Absolute glucose consumption rate within 24 h in SNU-638 cells expressing scramble or RB1 shRNA. Paired student’s t-test was performed. * P < 0.05. P Absolute lactate secretion rate within 24 h in SNU-638 cells expressing scramble or RB1 shRNA. Paired student’s t-test was performed. * P < 0.05. Q Relative incorporation of [U- 14 C]-glucose into lipid in AGS cells expressing scramble or RB1 shRNA those were cultured in the presence of [U- 14 C]-glucose (6.25 μCi/ml) for 24 h. 14 C activity was measured using liquid scintillation counter. Tukey HSD test was performed. ** P < 0.01 and * P < 0.05. R Effect of PGAM1 overexpression on glycolytic flow in RB1-depleted AGS cells. S Effect of PGAM1 overexpression on glucose consumption for 24 h in RB1-depleted AGS cells. Glucose concentration in medium was determined using colorimetric assay. Tukey HSD test was performed. * P < 0.05. All data are shown as the mean ± SEM.

    Journal: Cell Death & Disease

    Article Title: RB1 controls differentiation through positive regulation of phosphoglycerate mutases

    doi: 10.1038/s41419-025-07850-3

    Figure Lengend Snippet: A OCR, an index of oxygen consumption in mitochondria indicating oxidative phosphorylation coupled with glycolytic activity, was measured in 53KOLS cells expressing scramble or Rb shRNA. Cells were sequentially treated with 10.5 mM glucose and 100 mM 2-deoxy glucose. B ECAR, an index of the rate of lactate production indicating glycolytic activity, was measured in 53KOLS cells expressing scramble or Rb shRNA. Cells were sequentially treated with 10.5 mM glucose and 100 mM 2-deoxy glucose (2-DG). C Relative 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG) fluorescence intensity showing glucose uptake in 53KOLS cells expressing scramble or Rb shRNA. Paired student’s t-test was performed. * P < 0.05 and n.s., not significant. D ECAR was measured in 53KOLS cells expressing scramble or Rb shRNA following the treatment with 1 mM pyruvate. E ECAR was measured in 53KOLS. cells expressing scramble or Rb shRNA following the treatment with 4 mM glutamine. F OCR in 53KOLS cells expressing scramble or Rb shRNA was measured in the presence or absence of a glutaminase inhibitor, BPTES (5 μM). Tukey HSD test was performed. ** P < 0.01 and n.s., not significant. G Relative incorporation of [U- 14 C]-glucose into lipid. 53KOLS cells were cultured in the presence of [U- 14 C]-glucose (6.25 μCi/ml) for 24 h. 14 C activity was measured using liquid scintillation counter. Paired student’s t-test was performed. *** P < 0.001. H Relative incorporation of [U- 14 C]-glutamine into lipid. 53KOLS cells were cultured in the presence of [U- 14 C]-glutamine (62.5 μCi/ml) for 24 h. Paired student’s t-test was performed. n.s., not significant. I Relative lipid incorporation of 3 H 2 O. 53KOLS cells were cultured in the presence of 3 H 2 O (20 v/v%) for 24 h. 3 H activity was measured using liquid scintillation counter. Paired student’s t-test was performed. * P < 0.05. J IB of the indicated proteins in 53KOLS cells overexpressing Pgam2. K Relative lipid incorporation of [U- 14 C]-glucose in 53KOLS cells overexpressing Pgam2. Tukey’s HSD test was performed. * P < 0.05 and n.s., not significant. L ECAR was measured in AGS cells expressing scramble or RB1 shRNA. Cells were sequentially treated with 10.5 mM glucose and 100 mM 2-DG. M Relative 2-NBDG fluorescence intensity in AGS cells expressing scramble or RB1 shRNA. Tukey HSD test was performed. *** P < 0.001 and ** P < 0.01. N Relative 2-NBDG fluorescence intensity in SNU-638 cells expressing scramble or RB1 shRNA. Tukey HSD test was performed. ** P < 0.01 and ** P < 0.05. O Absolute glucose consumption rate within 24 h in SNU-638 cells expressing scramble or RB1 shRNA. Paired student’s t-test was performed. * P < 0.05. P Absolute lactate secretion rate within 24 h in SNU-638 cells expressing scramble or RB1 shRNA. Paired student’s t-test was performed. * P < 0.05. Q Relative incorporation of [U- 14 C]-glucose into lipid in AGS cells expressing scramble or RB1 shRNA those were cultured in the presence of [U- 14 C]-glucose (6.25 μCi/ml) for 24 h. 14 C activity was measured using liquid scintillation counter. Tukey HSD test was performed. ** P < 0.01 and * P < 0.05. R Effect of PGAM1 overexpression on glycolytic flow in RB1-depleted AGS cells. S Effect of PGAM1 overexpression on glucose consumption for 24 h in RB1-depleted AGS cells. Glucose concentration in medium was determined using colorimetric assay. Tukey HSD test was performed. * P < 0.05. All data are shown as the mean ± SEM.

    Article Snippet: Taqman probes used are as follow: mouse Actb (Mm00607939_s1), mouse Rb (Mm00485586_m1), mouse Hprt (Mm00446968_m1), mouse Nanog (Mm02019550_s1), mouse Pgam2 (Mm01187768_m1), mouse Myh7 (Mm00600555_m1), mouse Myog (Mm00446194_m1), mouse Myod1 (Mm00440387_m1) human ACTB (Hs99999903_m1), human RB (Hs01078066_m1), human PGAM1 (Hs01652468_g1) and human HPRT (Hs02800695_m1).

    Techniques: Phospho-proteomics, Activity Assay, Expressing, shRNA, Fluorescence, Cell Culture, Over Expression, Concentration Assay, Colorimetric Assay

    A Phase-contrast image of spheres developed from Rb-depleted 53KOLS cells expressing LacZ or PGAM2. B Number of spheres developed from Rb-depleted 53KOLS cells expressing LacZ or PGAM2. C mRNA expression of the indicated gene in monolayer or spheroid. Tukey’s HSD test was performed. **** P < 0.0001. D Phase-contrast image of spheres developed from AGS cells expressing scramble or RB1 shRNA. Scale bar; 200 μm. E Number of spheres developed from RB1-depleted AGS cells under 3D culture conditions. Tukey’s HSD test was performed. ** P < 0.01 and * P < 0.05. F Phase-contrast image of spheres developed from AGS cells expressing LacZ or PGAM1, those with or without RB1-depletion. G Number of spheres developed from the indicated cells cultured under the indicated 3D conditions. H Phase-contrast image of spheres developed from AGS cells expressing scramble or PGAM1 shRNA. I Number of spheres developed from PGAM1-depleted AGS cells under 3D culture conditions. Tukey’s HSD test was performed. ** P < 0.01. All data are shown as the mean ± SEM.

    Journal: Cell Death & Disease

    Article Title: RB1 controls differentiation through positive regulation of phosphoglycerate mutases

    doi: 10.1038/s41419-025-07850-3

    Figure Lengend Snippet: A Phase-contrast image of spheres developed from Rb-depleted 53KOLS cells expressing LacZ or PGAM2. B Number of spheres developed from Rb-depleted 53KOLS cells expressing LacZ or PGAM2. C mRNA expression of the indicated gene in monolayer or spheroid. Tukey’s HSD test was performed. **** P < 0.0001. D Phase-contrast image of spheres developed from AGS cells expressing scramble or RB1 shRNA. Scale bar; 200 μm. E Number of spheres developed from RB1-depleted AGS cells under 3D culture conditions. Tukey’s HSD test was performed. ** P < 0.01 and * P < 0.05. F Phase-contrast image of spheres developed from AGS cells expressing LacZ or PGAM1, those with or without RB1-depletion. G Number of spheres developed from the indicated cells cultured under the indicated 3D conditions. H Phase-contrast image of spheres developed from AGS cells expressing scramble or PGAM1 shRNA. I Number of spheres developed from PGAM1-depleted AGS cells under 3D culture conditions. Tukey’s HSD test was performed. ** P < 0.01. All data are shown as the mean ± SEM.

    Article Snippet: Taqman probes used are as follow: mouse Actb (Mm00607939_s1), mouse Rb (Mm00485586_m1), mouse Hprt (Mm00446968_m1), mouse Nanog (Mm02019550_s1), mouse Pgam2 (Mm01187768_m1), mouse Myh7 (Mm00600555_m1), mouse Myog (Mm00446194_m1), mouse Myod1 (Mm00440387_m1) human ACTB (Hs99999903_m1), human RB (Hs01078066_m1), human PGAM1 (Hs01652468_g1) and human HPRT (Hs02800695_m1).

    Techniques: Expressing, shRNA, Cell Culture

    A Heatmap of genes related to glycolysis in a time course microarray sampled during differentiation in C2C12 cells. B IB of the indicated proteins in differentiated and undifferentiated C2C12 cells. C mRNA expression levels of the indicated genes in C2C12 cells expressing scramble or Rb shRNA. C2C12 cells were differentiated under low serum conditions for 7 days. Tukey’s HSD test was performed. **** P < 0.0001 and * P < 0.05. D IB of the indicated proteins in C2C12 cells expressing scramble or Pgam2 shRNA those with or without exposure to stimuli to induce myogenic differentiation. E Immunocytochemistry of myosin heavy chain (MHC) in C2C12 cells expressing scramble or Pgam2 shRNA and exposed to stimuli to induce myogenic differentiation for 7 days. F mRNA expression levels of the indicated genes in C2C12 cells expressing scramble or Rb shRNA. Cells were differentiated in the presence or absence of pyruvate for 7 days. Tukey’s HSD test was performed. ** P < 0.01, * P < 0.05 and n.s.; not significant. G RNA-seq heatmap of genes related to glycolysis during adipogenic differentiation in 3T3L1 cells. H IB of the indicated proteins in differentiated and undifferentiated 3T3L1 cells. I IB of the indicated proteins in PGAM1-depleted 3T3L1 after exposure to stimuli to induce adipogenic differentiation. J Oil red staining of 3T3L1 cells transduced with the indicated gRNA and treated with vehicle or 4 mM pyruvate under the culture conditions to induce adipogenic differentiation. K IB of the indicated proteins in PGAM1-depleted 3T3L1 cells after exposure to stimuli to induce adipogenic differentiation in the presence or absence of 4 mM pyruvate. All data are shown as the mean ± SEM.

    Journal: Cell Death & Disease

    Article Title: RB1 controls differentiation through positive regulation of phosphoglycerate mutases

    doi: 10.1038/s41419-025-07850-3

    Figure Lengend Snippet: A Heatmap of genes related to glycolysis in a time course microarray sampled during differentiation in C2C12 cells. B IB of the indicated proteins in differentiated and undifferentiated C2C12 cells. C mRNA expression levels of the indicated genes in C2C12 cells expressing scramble or Rb shRNA. C2C12 cells were differentiated under low serum conditions for 7 days. Tukey’s HSD test was performed. **** P < 0.0001 and * P < 0.05. D IB of the indicated proteins in C2C12 cells expressing scramble or Pgam2 shRNA those with or without exposure to stimuli to induce myogenic differentiation. E Immunocytochemistry of myosin heavy chain (MHC) in C2C12 cells expressing scramble or Pgam2 shRNA and exposed to stimuli to induce myogenic differentiation for 7 days. F mRNA expression levels of the indicated genes in C2C12 cells expressing scramble or Rb shRNA. Cells were differentiated in the presence or absence of pyruvate for 7 days. Tukey’s HSD test was performed. ** P < 0.01, * P < 0.05 and n.s.; not significant. G RNA-seq heatmap of genes related to glycolysis during adipogenic differentiation in 3T3L1 cells. H IB of the indicated proteins in differentiated and undifferentiated 3T3L1 cells. I IB of the indicated proteins in PGAM1-depleted 3T3L1 after exposure to stimuli to induce adipogenic differentiation. J Oil red staining of 3T3L1 cells transduced with the indicated gRNA and treated with vehicle or 4 mM pyruvate under the culture conditions to induce adipogenic differentiation. K IB of the indicated proteins in PGAM1-depleted 3T3L1 cells after exposure to stimuli to induce adipogenic differentiation in the presence or absence of 4 mM pyruvate. All data are shown as the mean ± SEM.

    Article Snippet: Taqman probes used are as follow: mouse Actb (Mm00607939_s1), mouse Rb (Mm00485586_m1), mouse Hprt (Mm00446968_m1), mouse Nanog (Mm02019550_s1), mouse Pgam2 (Mm01187768_m1), mouse Myh7 (Mm00600555_m1), mouse Myog (Mm00446194_m1), mouse Myod1 (Mm00440387_m1) human ACTB (Hs99999903_m1), human RB (Hs01078066_m1), human PGAM1 (Hs01652468_g1) and human HPRT (Hs02800695_m1).

    Techniques: Microarray, Expressing, shRNA, Immunocytochemistry, RNA Sequencing, Staining, Transduction

    A Expression levels of Pgam2 in Myod-depleted C2C12 cells exposed to stimuli to induce myogenic differentiation. Expression levels of Pgam2 from microarray were shown in log 2 Fc. B Expression levels of myogenic transcription factors in 53KOLS cells. Data from RNA-seq was shown in Log 2 RPKM. C IB of the indicated protein in Mef2a or Mef2d-depleted 53KOLS cells. D Relative mRNA levels of the indicated genes in 53KOLS cells expressing scramble, Mef2a, or Mef2d shRNA. Data are shown as the mean ± standard error (SEM). Tukey HSD test was performed. *** P < 0.001, ** P < 0.01, * P < 0.05 and n.s., not significant. E Correlation of mRNA expression level between MEF2A/D and PGAM1 genes in TCGA dataset. Spearman’s correlation test was performed. F Sequence of the promoter region of mouse Pgam2 gene. G ChIP-qPCR assessment of H3K4me2 at the Mef2 binding site in the Pgam2 promoter of 53KOLS cells, those with or without Rb depletion. Paired student’s t-test was performed. ** P < 0.01. All data are shown as the mean ± SEM.

    Journal: Cell Death & Disease

    Article Title: RB1 controls differentiation through positive regulation of phosphoglycerate mutases

    doi: 10.1038/s41419-025-07850-3

    Figure Lengend Snippet: A Expression levels of Pgam2 in Myod-depleted C2C12 cells exposed to stimuli to induce myogenic differentiation. Expression levels of Pgam2 from microarray were shown in log 2 Fc. B Expression levels of myogenic transcription factors in 53KOLS cells. Data from RNA-seq was shown in Log 2 RPKM. C IB of the indicated protein in Mef2a or Mef2d-depleted 53KOLS cells. D Relative mRNA levels of the indicated genes in 53KOLS cells expressing scramble, Mef2a, or Mef2d shRNA. Data are shown as the mean ± standard error (SEM). Tukey HSD test was performed. *** P < 0.001, ** P < 0.01, * P < 0.05 and n.s., not significant. E Correlation of mRNA expression level between MEF2A/D and PGAM1 genes in TCGA dataset. Spearman’s correlation test was performed. F Sequence of the promoter region of mouse Pgam2 gene. G ChIP-qPCR assessment of H3K4me2 at the Mef2 binding site in the Pgam2 promoter of 53KOLS cells, those with or without Rb depletion. Paired student’s t-test was performed. ** P < 0.01. All data are shown as the mean ± SEM.

    Article Snippet: Taqman probes used are as follow: mouse Actb (Mm00607939_s1), mouse Rb (Mm00485586_m1), mouse Hprt (Mm00446968_m1), mouse Nanog (Mm02019550_s1), mouse Pgam2 (Mm01187768_m1), mouse Myh7 (Mm00600555_m1), mouse Myog (Mm00446194_m1), mouse Myod1 (Mm00440387_m1) human ACTB (Hs99999903_m1), human RB (Hs01078066_m1), human PGAM1 (Hs01652468_g1) and human HPRT (Hs02800695_m1).

    Techniques: Expressing, Microarray, RNA Sequencing, shRNA, Sequencing, ChIP-qPCR, Binding Assay

    Primary antibodies and secondary antibody utilized in Western blotting.

    Journal: Animals : an Open Access Journal from MDPI

    Article Title: Pyrroroquinoline Quinone (PQQ) Improves the Quality of Holstein Bull Semen during Cryopreservation

    doi: 10.3390/ani14202940

    Figure Lengend Snippet: Primary antibodies and secondary antibody utilized in Western blotting.

    Article Snippet: , PGAM2 , Rabbit , 1:2000 , Solarbio , K005552P.

    Techniques: Western Blot

    Impact of PQQ treatment on sperm protein consumption during freezing and thawing processes. ( A ) Western blot analysis was conducted to assess the protein levels of key proteins, including PGAM2, CAPZB, CAT, SOD1, and GPX1. ( B ) Grayscale scanning was utilized to quantify the abundance of these proteins (PGAM2, CAPZB, CAT, SOD1, and GPX1). Control: no PQQ treatment; PQQ: 50, 100, 150 µmol/L PQQ/1.0 × 10 8 sperm. Data are presented as mean ± SD ( n = 3); columns with different lowercase letters indicate significant differences ( p < 0.05).

    Journal: Animals : an Open Access Journal from MDPI

    Article Title: Pyrroroquinoline Quinone (PQQ) Improves the Quality of Holstein Bull Semen during Cryopreservation

    doi: 10.3390/ani14202940

    Figure Lengend Snippet: Impact of PQQ treatment on sperm protein consumption during freezing and thawing processes. ( A ) Western blot analysis was conducted to assess the protein levels of key proteins, including PGAM2, CAPZB, CAT, SOD1, and GPX1. ( B ) Grayscale scanning was utilized to quantify the abundance of these proteins (PGAM2, CAPZB, CAT, SOD1, and GPX1). Control: no PQQ treatment; PQQ: 50, 100, 150 µmol/L PQQ/1.0 × 10 8 sperm. Data are presented as mean ± SD ( n = 3); columns with different lowercase letters indicate significant differences ( p < 0.05).

    Article Snippet: , PGAM2 , Rabbit , 1:2000 , Solarbio , K005552P.

    Techniques: Western Blot, Control