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Structured Review

Proteintech pex3
Pex3, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pex3/PEX3+Antibody/pm41595693-84-26-29
Average 93 stars, based on 13 article reviews
pex3 - by Bioz Stars, 2026-09
93/100 stars

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Related Articles

Incubation:

Article Title: Integrated Multi-Omics Analysis Reveals Dysregulated Lipid Metabolism as a Novel Mechanism in Androgenetic Alopecia.
Article Snippet: .. After deparaffinization and antigen retrieval, sections were blocked and incubated with primary antibodies ACAA1 (1:50, 12319-2-AP, Proteintech, Rosemont, IL, USA), IDH1 (1:500, ab256557, Abcam, Cambridge, UK), PEX3 (1:200, 30424-1-AP, Proteintech), and DBI (1:4000, ab317823, Abcam) at 4 ◦C overnight. ..

Article Title: Integrated Multi-Omics Analysis Reveals Dysregulated Lipid Metabolism as a Novel Mechanism in Androgenetic Alopecia
Article Snippet: .. After deparaffinization and antigen retrieval, sections were blocked and incubated with primary antibodies ACAA1 (1:50, 12319-2-AP, Proteintech, Rosemont, IL, USA), IDH1 (1:500, ab256557, Abcam, Cambridge, UK), PEX3 (1:200, 30424-1-AP, Proteintech), and DBI (1:4000, ab317823, Abcam) at 4 °C overnight. ..

Article Title: PEX3 promotes regenerative repair after myocardial injury in mice through facilitating plasma membrane localization of ITGB3
Article Snippet: The prepared protein was separated in SDS-PAGE gel and then transferred to a polyvinylidene fluoride membrane (Millipore). .. After blocking with 5% bovine serum albumin for 2 h at room temperature, the blots were incubated with primary antibodies at 4 °C overnight, then conjugated to second antibodies at room temperature for 2 h. The primary antibodies used in this study are as follows: GAPDH (Proteintech, 10494-1-AP, diluted 1:1000), PMP70 (Abcam, ab109448, diluted 1:1000), PEX12 (Proteintech, 27011-1-AP, diluted 1:1000), PEX3 (Proteintech, 10946-1-AP, diluted 1:1000), Histone H3 (Abcam, ab1791, diluted 1:1000), Caveolin-1 (Abcam, ab32577, diluted 1:1000), ATP1A3 (Proteintech, 25727-1-AP, diluted 1:1000), ITGB3 (Proteintech, 18309-1-AP, diluted 1:1000), AKT (CST, 4691S, diluted in 1:1000). p-AKT S473 (CST, 4060S, diluted 1:1000), p-AKT T308 (CST, 13038S, diluted 1:1000), p-PTEN S380(CST, 9551S, diluted 1:1000), p-PDK1 S241 (CST, 3438S, diluted 1:1000), β-catenin (Abcam, ab223075, diluted 1:1000) GSK-3β (Abcam, ab32391, diluted 1:1000), p-GSK-3β (CST, 5558S, diluted 1:1000). ..

Article Title: PEX3 promotes regenerative repair after myocardial injury in mice through facilitating plasma membrane localization of ITGB3.
Article Snippet: The prepared protein was separated in SDS-PAGE gel and then transferred to a polyvinylidene fluoride membrane (Millipore). .. After blocking with 5% bovine serum albumin for 2 h at room temperature, the blots were incubated with primary antibodies at 4 °C overnight, then conjugated to second antibodies at room temperature for 2 h. The primary antibodies used in this study are as follows: GAPDH (Proteintech, 10494-1-AP, diluted 1:1000), PMP70 (Abcam, ab109448, diluted 1:1000), PEX12 (Proteintech, 27011-1-AP, diluted 1:1000), PEX3 (Proteintech, 10946-1-AP, diluted 1:1000), Histone H3 (Abcam, ab1791, diluted 1:1000), Caveolin-1 (Abcam, ab32577, diluted 1:1000), ATP1A3 (Proteintech, 25727-1-AP, diluted 1:1000), ITGB3 (Proteintech, 18309-1-AP, diluted 1:1000), AKT (CST, 4691S, diluted in 1:1000). p-AKT S473 (CST, 4060S, diluted 1:1000), p-AKT T308 (CST, 13038S, diluted 1:1000), p-PTEN S380(CST, 9551S, diluted 1:1000), p-PDK1 S241 (CST, 3438S, diluted 1:1000),β-catenin (Abcam, ab223075, diluted 1:1000)GSK-3β (Abcam, ab32391, diluted 1:1000), p-GSK-3β (CST, 5558S, diluted 1:1000). ..

Western Blot:

Article Title: The p97 ATPase and its adaptor UBXD8 maintain peroxisome pools by preventing pexophagy.
Article Snippet: Peroxisomes perform key metabolic functions in eukaryotic cells.. Loss of peroxisome function causes peroxisome biogenesis disorders and severe childhood diseases with disrupted lipid metabolism.. One mechanism regulating peroxisome abundance is degradation via selective autophagy (pexophagy).

Article Title: The p97-UBXD8 complex maintains peroxisome abundance by suppressing pexophagy
Article Snippet: The supernatant was collected, and protein concentration was estimated using the DC protein assay kit (Biorad). .. The p97 (10736-1-AP; WB: 1:2000), UBXD8 (16251-1-AP; WB: 1:2000), UBXD2 (21052-1-AP; WB: 1:2000), HRD1 (13473-1-AP; WB: 1:2000), AMFR/GP78 (16675-1-AP; WB: 1:2000), VAPB (14477-1-AP; WB: 1:2000), PEX5 ( ) (12545-1-AP; WB 1:500), PEX19 (14713-1-AP; WB 1:1000), MLYCD (15265-1-AP; WB 1:2000), PECR (14901-1-AP; WB 1:1000), DECR (25855-1-AP; WB 1:1000), PMP70/ABCD3 (66697-1-Ig; WB 1:1000; IF 1:400), PEX3 (10946-1-AP; WB 1:1000), ACBD5 (21080-1-AP; WB 1:1000), GFP(66002-1-AP; WB: 1:2000) and ATG5 (10181-2-AP; WB 1:1000) antibodies were from Proteintech Inc. .. The pan-ubiquitin (P4D1; sc8017; WB: 1:2000), c-Myc (9E10; sc40; WB: 1:2000), β-Actin (AC-15; sc69879; WB: 1:2000), and GAPDH (O411; sc47724; WB: 1:2000) antibodies were obtained from Santa Cruz Biotechnologies.

Blocking Assay:

Article Title: PEX3 promotes regenerative repair after myocardial injury in mice through facilitating plasma membrane localization of ITGB3
Article Snippet: The prepared protein was separated in SDS-PAGE gel and then transferred to a polyvinylidene fluoride membrane (Millipore). .. After blocking with 5% bovine serum albumin for 2 h at room temperature, the blots were incubated with primary antibodies at 4 °C overnight, then conjugated to second antibodies at room temperature for 2 h. The primary antibodies used in this study are as follows: GAPDH (Proteintech, 10494-1-AP, diluted 1:1000), PMP70 (Abcam, ab109448, diluted 1:1000), PEX12 (Proteintech, 27011-1-AP, diluted 1:1000), PEX3 (Proteintech, 10946-1-AP, diluted 1:1000), Histone H3 (Abcam, ab1791, diluted 1:1000), Caveolin-1 (Abcam, ab32577, diluted 1:1000), ATP1A3 (Proteintech, 25727-1-AP, diluted 1:1000), ITGB3 (Proteintech, 18309-1-AP, diluted 1:1000), AKT (CST, 4691S, diluted in 1:1000). p-AKT S473 (CST, 4060S, diluted 1:1000), p-AKT T308 (CST, 13038S, diluted 1:1000), p-PTEN S380(CST, 9551S, diluted 1:1000), p-PDK1 S241 (CST, 3438S, diluted 1:1000), β-catenin (Abcam, ab223075, diluted 1:1000) GSK-3β (Abcam, ab32391, diluted 1:1000), p-GSK-3β (CST, 5558S, diluted 1:1000). ..

Article Title: PEX3 promotes regenerative repair after myocardial injury in mice through facilitating plasma membrane localization of ITGB3.
Article Snippet: The prepared protein was separated in SDS-PAGE gel and then transferred to a polyvinylidene fluoride membrane (Millipore). .. After blocking with 5% bovine serum albumin for 2 h at room temperature, the blots were incubated with primary antibodies at 4 °C overnight, then conjugated to second antibodies at room temperature for 2 h. The primary antibodies used in this study are as follows: GAPDH (Proteintech, 10494-1-AP, diluted 1:1000), PMP70 (Abcam, ab109448, diluted 1:1000), PEX12 (Proteintech, 27011-1-AP, diluted 1:1000), PEX3 (Proteintech, 10946-1-AP, diluted 1:1000), Histone H3 (Abcam, ab1791, diluted 1:1000), Caveolin-1 (Abcam, ab32577, diluted 1:1000), ATP1A3 (Proteintech, 25727-1-AP, diluted 1:1000), ITGB3 (Proteintech, 18309-1-AP, diluted 1:1000), AKT (CST, 4691S, diluted in 1:1000). p-AKT S473 (CST, 4060S, diluted 1:1000), p-AKT T308 (CST, 13038S, diluted 1:1000), p-PTEN S380(CST, 9551S, diluted 1:1000), p-PDK1 S241 (CST, 3438S, diluted 1:1000),β-catenin (Abcam, ab223075, diluted 1:1000)GSK-3β (Abcam, ab32391, diluted 1:1000), p-GSK-3β (CST, 5558S, diluted 1:1000). ..



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