permeabilization buffer (Complete Genomics Inc)
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Complete Genomics Inc
permeabilization buffer
Permeabilization Buffer, supplied by Complete Genomics Inc, used in various techniques. Bioz Stars score: 99/100, based on 495 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/permeabilization+buffer+set/Stereo-seq+Transcriptomics+Set+for+FFPE/pmc12709731-202-13-17
Average 99 stars, based on 495 article reviews
Permeabilization Buffer, supplied by Complete Genomics Inc, used in various techniques. Bioz Stars score: 99/100, based on 495 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/permeabilization+buffer+set/Stereo-seq+Transcriptomics+Set+for+FFPE/pmc12709731-202-13-17
Average 99 stars, based on 495 article reviews
permeabilization buffer - by Bioz Stars,
2026-11
99/100 stars
Images
1) Product Images from "ST-FFPE-mIF: integrating spatial transcriptomics and multiplex immunofluorescence in formalin-fixed paraffin-embedded tissues using Stereo-seq"
Article Title: ST-FFPE-mIF: integrating spatial transcriptomics and multiplex immunofluorescence in formalin-fixed paraffin-embedded tissues using Stereo-seq
Journal: Genome Biology
doi: 10.1186/s13059-025-03900-3
Figure Legend Snippet: Schematic workflow and bioinformatics analysis of the ST-FFPE-mIF whole solution. (1) FFPE tissue section was mounted on the chip and performed de-paraffinization (2) The section on the chip was subjected to antigen repair, blocking, antibody incubation, and mIF imaging. Before the 2nd round of mIF staining, antibodies from the 1 st round should be removal, and the epitope would be re-exposed. Then, the above mIF protocol should be repeated to generate another mIF image. Nine-colored mIF images were obtained accordingly (3) Permeabilization, in situ cDNA synthesis and collection were conducted after De-crosslink (4) cDNA amplification, library construction, and sequencing were performed according to manufacturer’s protocol to obtain Stereo-seq sequencing result (5) mIF images and spatially resolved gene expression was subjected to downstream spatial visualization and bioinformatics analyses including image processing and spatial multi-omics analysis to address scientific questions
Techniques Used: Blocking Assay, Incubation, Imaging, Staining, In Situ, cDNA Synthesis, Amplification, Sequencing, Gene Expression, Biomarker Discovery
Related Articles
Incubation:Article Title: Stereo-seq of the prefrontal cortex in aging and Alzheimer’s disease Article Snippet: The nuclei images were captured using a Zeiss Axio Scan Z1 microscope (at EGFP wavelength). .. Subsequently, the tissue section was incubated in the Article Title: ST-FFPE-mIF: integrating spatial transcriptomics and multiplex immunofluorescence in formalin-fixed paraffin-embedded tissues using Stereo-seq Article Snippet: Fixation in pre-cooled methanol (Cat# 34860, Sigma) for 30 min at − 20 °C ensued. .. Post-fixation, the Stereo-seq chip was air-dried, and the tissue section was incubated in Article Title: Stereo-seq of the prefrontal cortex in aging and Alzheimer's disease. Article Snippet: .. Subsequently, the tissue section was incubated in the Article Title: ST-FFPE-mIF: Integrating Spatial Transcriptomics and Multiplex Immunofluorescence in Formalin-Fixed Paraffin-Embedded Tissues Using Stereo-seq Article Snippet: Fixation in pre-cooled methanol (Cat# 34860, Sigma) for 30 min at −20°C ensued. .. Post-fixation, the Stereo-seq chip was air-dried, and the tissue section was incubated in Article Title: ST-FFPE-mIF: integrating spatial transcriptomics and multiplex immunofluorescence in formalin-fixed paraffin-embedded tissues using Stereo-seq. Article Snippet: Fixation in pre-cooled methanol (Cat# 34860, Sigma) for 30 min at − 20 °C ensued. .. Post-fixation, the Stereo-seq chip was air-dried, and the tissue section was incubated in other:Article Title: Psychedelic control of neuroimmune interactions governing fear. Article Snippet: Elizabeth N. Chung, Jinsu Lee, Carolina M. Polonio, Joshua Choi, Camilo Faust Akl, Michael Kilian, Wiebke M. Weiß, Georgia Gunner, Mingyu Ye, Tae Hyun Heo, Sienna S. Drake, Liu Yang, Catarina R. G. L. d’Eca, Joon-Hyuk Lee, Liwen Deng, Daniel Farrenkopf, Anton M. Schüle, Hong-Gyun Lee, Oreoluwa Afolabi, Sharmin Ghaznavi, Stelios M. Smirnakis, Isaac M. Chiu, Vijay K. Kuchroo, Francisco J. Quintana & Michael A. Wheeler1,2 ✉ Article Title: Morphogenic, molecular and cellular adaptations for unidirectional airflow in the chicken lung. Article Snippet: Briefly, the tissue section on the Stereo-seq chip (1 cm×1 cm) was incubated at 37°C for 5 min and subsequently fixed in pre-cooled methanol (34860, Sigma-Aldrich; precooled for 30 mins at −20°C) at −20°C. Transcriptomics:Article Title: Quasi-spatial single-cell transcriptome based on physical tissue properties defines early aging associated niche in liver. Article Snippet: Aging is associated with the accumulation of senescent cells, which are triggered by tissue injury response and often escape clearance by the immune system.. The specific traits and diversity of these cells in aged tissues, along with their effects on the t is sue m ic ro en vi ro nment, remain largely unexplored.. Despite the advances in single-cell and spatial omics technologies to understand complex tissue architecture, senescent cell populations are often neglected in general analysis pipelines due to their scarcity and the technical bias in current omics toolkits. |