permeabilization buffer (Complete Genomics Inc)
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Complete Genomics Inc
permeabilization buffer
Permeabilization Buffer, supplied by Complete Genomics Inc, used in various techniques. Bioz Stars score: 99/100, based on 495 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/permeabilization+buffer+set/Stereo-seq+Transcriptomics+Set+for+FFPE/pmc12709731-202-13-17
Average 99 stars, based on 495 article reviews
Permeabilization Buffer, supplied by Complete Genomics Inc, used in various techniques. Bioz Stars score: 99/100, based on 495 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/permeabilization+buffer+set/Stereo-seq+Transcriptomics+Set+for+FFPE/pmc12709731-202-13-17
Average 99 stars, based on 495 article reviews
permeabilization buffer - by Bioz Stars,
2026-11
99/100 stars
Images
1) Product Images from "ST-FFPE-mIF: integrating spatial transcriptomics and multiplex immunofluorescence in formalin-fixed paraffin-embedded tissues using Stereo-seq"
Article Title: ST-FFPE-mIF: integrating spatial transcriptomics and multiplex immunofluorescence in formalin-fixed paraffin-embedded tissues using Stereo-seq
Journal: Genome Biology
doi: 10.1186/s13059-025-03900-3
Figure Legend Snippet: Schematic workflow and bioinformatics analysis of the ST-FFPE-mIF whole solution. (1) FFPE tissue section was mounted on the chip and performed de-paraffinization (2) The section on the chip was subjected to antigen repair, blocking, antibody incubation, and mIF imaging. Before the 2nd round of mIF staining, antibodies from the 1 st round should be removal, and the epitope would be re-exposed. Then, the above mIF protocol should be repeated to generate another mIF image. Nine-colored mIF images were obtained accordingly (3) Permeabilization, in situ cDNA synthesis and collection were conducted after De-crosslink (4) cDNA amplification, library construction, and sequencing were performed according to manufacturer’s protocol to obtain Stereo-seq sequencing result (5) mIF images and spatially resolved gene expression was subjected to downstream spatial visualization and bioinformatics analyses including image processing and spatial multi-omics analysis to address scientific questions
Techniques Used: Blocking Assay, Incubation, Imaging, Staining, In Situ, cDNA Synthesis, Amplification, Sequencing, Gene Expression, Biomarker Discovery
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