pcl12  (DSMZ)

Journal: Cell Death & Disease

Article Title: Disrupting pro-survival and inflammatory pathways with dimethyl fumarate sensitizes chronic lymphocytic leukemia to cell death

doi: 10.1038/s41419-024-06602-z

Figure Lengend Snippet: A–D Growth curves of the indicated cell lines treated with increasing concentrations of DMF for 24 h, 48 h and 72 h, expressed as cell number per milliliter. E – H Cell viability was assessed with trypan blue exclusion method and automatic counting before and after 24 h, 48 h and 72 h of treatment with DMF; data are expressed as percentage of viable cells compared to that of untreated cells. n = 8 biological replicates for MEC1; n = 5 for the other cell lines for panels A-H. I Western blot analysis of proapoptotic and antiapoptotic proteins expression after DMF treatment (25, 50 and 100 µM); images representative of two independent experiments performed with MEC1 and MEC2 total cellular extracts. Beta-actin was used as internal control. J – M Metabolic activation in CLL cell lines exposed to increasing doses of DMF (25–100 µM) after 24 h, 48 h and 72 h; ATP content is expressed as percentage of relative luminescence units, RLU, versus untreated (MEC1, n = 8; MEC2, n = 5; HG3, n = 5; PCL12, n = 5). All the data are shown as the mean ± SD. Two-way ANOVA with Dunnett’s post hoc test was performed for multiple comparisons. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.

Article Snippet: The MEC1, MEC2, HG3 and PCL12 cell lines were purchased from DSMZ, cell bank (Cell Bank of the Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Braunschweig, Germany) and cultured in RPMI 1640 medium (Euroclone, Pero, Milano) supplemented with 10% heat-inactivated FBS and Pen-Strep (1000 U; Sigma‒Aldrich St Louis, MO, USA) at a concentration of 1 × 10 6 cells/ml for early treatments and at 8 × 10 5 for longer treatments.

Techniques: Western Blot, Expressing, Control, Activation Assay