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Bio-Rad pbst
Demonstration of Type 3 antibody specificity. A microtiter plate was coated overnight with the <t>omalizumab</t> target human IgE, omalizumab, human IgG1/κ or human IgG1/λ at a concentration of 5 µg/mL. After washing and blocking with <t>PBST</t> + 5% BSA, the omalizumab/human IgE complex was formed by adding 2 µg/mL omalizumab to the wells coated with human IgE. Detection was performed with HRP-conjugated Type 3 anti-omalizumab/hIgE antibody AbD20760 in HISPEC assay diluent, followed by QuantaBlu fluorogenic peroxidase substrate.
Pbst, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 807 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pbst/product/Bio-Rad
Average 94 stars, based on 807 article reviews
Price from $9.99 to $1999.99
pbst - by Bioz Stars, 2020-07
94/100 stars

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1) Product Images from "Generation by phage display and characterization of drug-target complex-specific antibodies for pharmacokinetic analysis of biotherapeutics"

Article Title: Generation by phage display and characterization of drug-target complex-specific antibodies for pharmacokinetic analysis of biotherapeutics

Journal: mAbs

doi: 10.1080/19420862.2018.1538723

Demonstration of Type 3 antibody specificity. A microtiter plate was coated overnight with the omalizumab target human IgE, omalizumab, human IgG1/κ or human IgG1/λ at a concentration of 5 µg/mL. After washing and blocking with PBST + 5% BSA, the omalizumab/human IgE complex was formed by adding 2 µg/mL omalizumab to the wells coated with human IgE. Detection was performed with HRP-conjugated Type 3 anti-omalizumab/hIgE antibody AbD20760 in HISPEC assay diluent, followed by QuantaBlu fluorogenic peroxidase substrate.
Figure Legend Snippet: Demonstration of Type 3 antibody specificity. A microtiter plate was coated overnight with the omalizumab target human IgE, omalizumab, human IgG1/κ or human IgG1/λ at a concentration of 5 µg/mL. After washing and blocking with PBST + 5% BSA, the omalizumab/human IgE complex was formed by adding 2 µg/mL omalizumab to the wells coated with human IgE. Detection was performed with HRP-conjugated Type 3 anti-omalizumab/hIgE antibody AbD20760 in HISPEC assay diluent, followed by QuantaBlu fluorogenic peroxidase substrate.

Techniques Used: Concentration Assay, Blocking Assay

2) Product Images from "Generation by phage display and characterization of drug-target complex-specific antibodies for pharmacokinetic analysis of biotherapeutics"

Article Title: Generation by phage display and characterization of drug-target complex-specific antibodies for pharmacokinetic analysis of biotherapeutics

Journal: mAbs

doi: 10.1080/19420862.2018.1538723

Demonstration of Type 3 antibody specificity. A microtiter plate was coated overnight with the omalizumab target human IgE, omalizumab, human IgG1/κ or human IgG1/λ at a concentration of 5 µg/mL. After washing and blocking with PBST + 5% BSA, the omalizumab/human IgE complex was formed by adding 2 µg/mL omalizumab to the wells coated with human IgE. Detection was performed with HRP-conjugated Type 3 anti-omalizumab/hIgE antibody AbD20760 in HISPEC assay diluent, followed by QuantaBlu fluorogenic peroxidase substrate.
Figure Legend Snippet: Demonstration of Type 3 antibody specificity. A microtiter plate was coated overnight with the omalizumab target human IgE, omalizumab, human IgG1/κ or human IgG1/λ at a concentration of 5 µg/mL. After washing and blocking with PBST + 5% BSA, the omalizumab/human IgE complex was formed by adding 2 µg/mL omalizumab to the wells coated with human IgE. Detection was performed with HRP-conjugated Type 3 anti-omalizumab/hIgE antibody AbD20760 in HISPEC assay diluent, followed by QuantaBlu fluorogenic peroxidase substrate.

Techniques Used: Concentration Assay, Blocking Assay

Related Articles

Concentration Assay:

Article Title: Generation by phage display and characterization of drug-target complex-specific antibodies for pharmacokinetic analysis of biotherapeutics
Article Snippet: .. Omalizumab at a fixed concentration (100 ng/mL = 0.7 nM) in PBST was incubated for 1 h with an increasing amount of human IgE (Bio-Rad HCA171) or NHS (1:2 dilution series with a starting concentration of 45% NHS) and then added to the plate with the immobilized antibody for 30 min. .. Detection of bound human IgE was performed with 2 µg/mL of HRP-conjugated anti-human IgE antibody (Bio-Rad 0100-0413P) in HISPEC assay diluent.

Incubation:

Article Title: Clinical trial in healthy malaria-naïve adults to evaluate the safety, tolerability, immunogenicity and efficacy of MuStDO5, a five-gene, sporozoite/hepatic stage Plasmodium falciparum DNA vaccine combined with escalating dose human GM-CSF DNA
Article Snippet: .. Again the plates were washed with PBST, incubated at room temperature for 1 h with 50 μL of secondary antibody [goat anti-human IgG (heavy and light chains) alkaline phosphatase-conjugate (AP), Bio-Rad 170–6521] diluted 1:1000 in 1% milk block. ..

Article Title: MSMEG_2731, an Uncharacterized Nucleic Acid Binding Protein from Mycobacterium smegmatis, Physically Interacts with RPS1
Article Snippet: .. After washing the membrane three times with 1× PBST, it was incubated with goat anti-Rabbit immunoglobulin G horseradish peroxidase-conjugated secondary antibody at room temperature for 1 h. The membrane was then washed three times with 1× PBST and incubated with chemiluminescent substrates (SuperSignal® West Pico, Bio-Rad). .. After exposing the blot to Fuji film for 30 seconds, the film was scanned and then analyzed by Quantity One® software.

Article Title: VPAC1 receptors play a dominant role in PACAP-induced vasorelaxation in female mice
Article Snippet: .. After washing three times for 10 min with PBST, membranes were incubated with the secondary anti-rabbit IgG antibody (Bio-Rad Laboratories, CA, USA) in 1:1500 dilution in PBST containing 1% non-fat dry milk for 2 h at room temperature. .. Signals were detected by enhanced chemiluminescence (Advansta Inc., Menlo Park, CA, USA) according to the instructions of the manufacturer.

Article Title: Generation by phage display and characterization of drug-target complex-specific antibodies for pharmacokinetic analysis of biotherapeutics
Article Snippet: .. Omalizumab at a fixed concentration (100 ng/mL = 0.7 nM) in PBST was incubated for 1 h with an increasing amount of human IgE (Bio-Rad HCA171) or NHS (1:2 dilution series with a starting concentration of 45% NHS) and then added to the plate with the immobilized antibody for 30 min. .. Detection of bound human IgE was performed with 2 µg/mL of HRP-conjugated anti-human IgE antibody (Bio-Rad 0100-0413P) in HISPEC assay diluent.

Article Title: Simple Sequence Repeats and Genome Plasticity in Streptococcus agalactiae ▿ ▿ †
Article Snippet: .. The membranes were next incubated for 1 h with PBST plus 1% bovine serum albumin (PBSTA) containing primary antibodies diluted 1:2,000, washed five times in PBST and incubated for 1 h with PBSTA containing secondary antibodies (goat anti-mouse horseradish peroxidase [HRP] conjugate; Bio-Rad) diluted 1:30,000; this was followed by another five washes in PBST. .. Protein bands reacting with the primary antibodies were visualized on film by using SuperSignal West Pico chemiluminescent substrate according to the manufacturer's instructions (Pierce).

Article Title: X-chromosome silencing in the germline of C. elegans
Article Snippet: .. They were blocked in PBST with 0.5 mg/ml bovine serum albumin (BSA) and incubated in a 1:200 dilution of H4Ac12 antibody (Serotec; Raleigh, NC) in block overnight at 4°C. .. After three washes in PBST, the samples were incubated in a 1:200 dilution of FITC-labeled anti-rabbit IgG (Jackson ImmunoResearch) overnight at 4°C.

Blocking Assay:

Article Title: Clinical trial in healthy malaria-naïve adults to evaluate the safety, tolerability, immunogenicity and efficacy of MuStDO5, a five-gene, sporozoite/hepatic stage Plasmodium falciparum DNA vaccine combined with escalating dose human GM-CSF DNA
Article Snippet: .. Again the plates were washed with PBST, incubated at room temperature for 1 h with 50 μL of secondary antibody [goat anti-human IgG (heavy and light chains) alkaline phosphatase-conjugate (AP), Bio-Rad 170–6521] diluted 1:1000 in 1% milk block. ..

Article Title: X-chromosome silencing in the germline of C. elegans
Article Snippet: .. They were blocked in PBST with 0.5 mg/ml bovine serum albumin (BSA) and incubated in a 1:200 dilution of H4Ac12 antibody (Serotec; Raleigh, NC) in block overnight at 4°C. .. After three washes in PBST, the samples were incubated in a 1:200 dilution of FITC-labeled anti-rabbit IgG (Jackson ImmunoResearch) overnight at 4°C.

Western Blot:

Article Title: The molecular logic of Nanog-induced self-renewal in mouse embryonic stem cells
Article Snippet: .. Membranes were washed three times in PBST and revealed with Clarity Max Western ECL Substrate (Biorad 1705062). .. Chemoluminescence was imaged on a ChemiDoc MP Imaging System (Biorad 1705062).

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    Bio-Rad pbst
    Demonstration of Type 3 antibody specificity. A microtiter plate was coated overnight with the <t>omalizumab</t> target human IgE, omalizumab, human IgG1/κ or human IgG1/λ at a concentration of 5 µg/mL. After washing and blocking with <t>PBST</t> + 5% BSA, the omalizumab/human IgE complex was formed by adding 2 µg/mL omalizumab to the wells coated with human IgE. Detection was performed with HRP-conjugated Type 3 anti-omalizumab/hIgE antibody AbD20760 in HISPEC assay diluent, followed by QuantaBlu fluorogenic peroxidase substrate.
    Pbst, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 807 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbst/product/Bio-Rad
    Average 94 stars, based on 807 article reviews
    Price from $9.99 to $1999.99
    pbst - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

    93
    Bio-Rad pbst buffer
    Demonstration of Type 3 antibody specificity. A microtiter plate was coated overnight with the <t>omalizumab</t> target human IgE, omalizumab, human IgG1/κ or human IgG1/λ at a concentration of 5 µg/mL. After washing and blocking with <t>PBST</t> + 5% BSA, the omalizumab/human IgE complex was formed by adding 2 µg/mL omalizumab to the wells coated with human IgE. Detection was performed with HRP-conjugated Type 3 anti-omalizumab/hIgE antibody AbD20760 in HISPEC assay diluent, followed by QuantaBlu fluorogenic peroxidase substrate.
    Pbst Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbst buffer/product/Bio-Rad
    Average 93 stars, based on 33 article reviews
    Price from $9.99 to $1999.99
    pbst buffer - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    Image Search Results


    Demonstration of Type 3 antibody specificity. A microtiter plate was coated overnight with the omalizumab target human IgE, omalizumab, human IgG1/κ or human IgG1/λ at a concentration of 5 µg/mL. After washing and blocking with PBST + 5% BSA, the omalizumab/human IgE complex was formed by adding 2 µg/mL omalizumab to the wells coated with human IgE. Detection was performed with HRP-conjugated Type 3 anti-omalizumab/hIgE antibody AbD20760 in HISPEC assay diluent, followed by QuantaBlu fluorogenic peroxidase substrate.

    Journal: mAbs

    Article Title: Generation by phage display and characterization of drug-target complex-specific antibodies for pharmacokinetic analysis of biotherapeutics

    doi: 10.1080/19420862.2018.1538723

    Figure Lengend Snippet: Demonstration of Type 3 antibody specificity. A microtiter plate was coated overnight with the omalizumab target human IgE, omalizumab, human IgG1/κ or human IgG1/λ at a concentration of 5 µg/mL. After washing and blocking with PBST + 5% BSA, the omalizumab/human IgE complex was formed by adding 2 µg/mL omalizumab to the wells coated with human IgE. Detection was performed with HRP-conjugated Type 3 anti-omalizumab/hIgE antibody AbD20760 in HISPEC assay diluent, followed by QuantaBlu fluorogenic peroxidase substrate.

    Article Snippet: Omalizumab at a fixed concentration (100 ng/mL = 0.7 nM) in PBST was incubated for 1 h with an increasing amount of human IgE (Bio-Rad HCA171) or NHS (1:2 dilution series with a starting concentration of 45% NHS) and then added to the plate with the immobilized antibody for 30 min.

    Techniques: Concentration Assay, Blocking Assay

    Demonstration of Type 3 antibody specificity. A microtiter plate was coated overnight with the omalizumab target human IgE, omalizumab, human IgG1/κ or human IgG1/λ at a concentration of 5 µg/mL. After washing and blocking with PBST + 5% BSA, the omalizumab/human IgE complex was formed by adding 2 µg/mL omalizumab to the wells coated with human IgE. Detection was performed with HRP-conjugated Type 3 anti-omalizumab/hIgE antibody AbD20760 in HISPEC assay diluent, followed by QuantaBlu fluorogenic peroxidase substrate.

    Journal: mAbs

    Article Title: Generation by phage display and characterization of drug-target complex-specific antibodies for pharmacokinetic analysis of biotherapeutics

    doi: 10.1080/19420862.2018.1538723

    Figure Lengend Snippet: Demonstration of Type 3 antibody specificity. A microtiter plate was coated overnight with the omalizumab target human IgE, omalizumab, human IgG1/κ or human IgG1/λ at a concentration of 5 µg/mL. After washing and blocking with PBST + 5% BSA, the omalizumab/human IgE complex was formed by adding 2 µg/mL omalizumab to the wells coated with human IgE. Detection was performed with HRP-conjugated Type 3 anti-omalizumab/hIgE antibody AbD20760 in HISPEC assay diluent, followed by QuantaBlu fluorogenic peroxidase substrate.

    Article Snippet: Omalizumab at a fixed concentration (100 ng/mL = 0.7 nM) in PBST was incubated for 1 h with an increasing amount of human IgE (Bio-Rad HCA171) or NHS (1:2 dilution series with a starting concentration of 45% NHS) and then added to the plate with the immobilized antibody for 30 min.

    Techniques: Concentration Assay, Blocking Assay